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            <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/dataset/3681.rdf" xlink:actuate="onRequest">Standard Stations - SINC from R/V Blue Heron cruises BH09-SINC1, BH10-01, BH10-06, BH10-13, and BH10-22 in the Lake Superior from 2009 to 2010 (SINC project)</gmx:Anchor>
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        <gco:CharacterString>Standard Stations - SINC Dataset Description: &amp;lt;p&amp;gt;The project research team identified named sampling locations to  facilitate repeat occupation of those locations and develop time-series  data sets.&amp;lt;/p&amp;gt;
&amp;lt;p&amp;gt;This is the list of all the standard stations occupied during the SINC Project.&amp;lt;/p&amp;gt;
&amp;lt;p&amp;gt;No attempt is made to make them cruise specific so any of the SINC deployments will return all of the SINC stations occupied.&amp;lt;/p&amp;gt;
&amp;lt;p&amp;gt;Cruise specific station locations can be retrieved from datasets such as  CTD Profiles or BioGeoChem using the CruiseId or CRUISE_CODE.&amp;lt;/p&amp;gt; Methods and Sampling: &amp;lt;p&amp;gt;Complied by Sterner, et al&amp;lt;/p&amp;gt;</gco:CharacterString>
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&lt;p&gt;Over large scales encompassing heterogeneous conditions, biogeochemical mechanisms act to achieve a stoichiometric balance between nitrogen and phosphorus. Locally, however, imbalances can develop. The Laurentian Great Lakes are a vast freshwater system where nitrate has been steadily accumulating for decades. Previous work has shown that in Lake Superior, the headwaters of the system, nitrate enters the lake water primarily due to in-lake biogeochemical processes, not due to passive accumulation of nitrate as a conservative substance as previously believed. An extreme stoichiometric imbalance of nitrate/phosphate ratios (~ 10,000 by moles) is present and is apparently growing. This set of prior findings opens up two major questions. First, what are the principal biogeochemical control points that tip the N cycle toward buildup of excess nitrate? And second, how does the extreme stoichiometric imbalance affect the ecology and evolution of Lake Superior's biota?&lt;/p&gt;
&lt;p&gt;In this project, researchers at the University of Minnesota - Twin Cities and the Bowling Green State University, who previously documented the nitrate buildup in Lake Superior, will continue their research program and address these two questions. The project is organized around making comparative measurements of N assimilation, nitrification, denitrification, anammox, and microbial community structure in Lake Superior and in the central basin of Lake Erie. These two environments differ greatly in many ways including redox state and organic carbon production rates. From the standpoint of N balancing mechanisms, they can be considered end members within the Laurentian Great Lakes. Additional data will be collected across a larger region of the Upper Great Lakes including Lake Huron. Up-to-date mass balance budgets of nitrogen of the most of the Great Lakes (Lake Superior is already done) will be constructed and linked with hydrologic fluxes to gain insights into the dynamics of N across the entire Laurentian Great Lakes System. Observations of water chemistry will be made with ship-board sampling together with field-deployed nitrate sensors in shallow and deep waters. Process studies will be performed in the water column and at the sediment-water interface and will involve sensitive stable isotope techniques. These will include measurements of NO3 and NH4+ uptake into different size fractions, exchanges of different forms of N and C between the water column and sediments, nitrification, denitrification, and anammox. The diversity and abundance of ammonia oxidizing Archea (AOA) and bacteria (AOB) will be studied using quantitative real time PCR and DGGE. Similarly, the genetic composition of denitrifyers and anammox bacteria will be studied to see if they too are represented by novel clades in Lake Superior. Cultured nitrifyers will be characterized in terms of growth under different conditions typically encountered across the Great Lakes. The project will yield valuable information and insight into the operation of the nitrogen cycle under conditions that promote stoichiometric imbalances.&lt;/p&gt;
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&lt;p&gt;The Laurentian Great Lakes are a valuable regional resource and an immense reservoir of planetary fresh water. Lake Superior is often considered to be relatively pristine but the ultimate source of the N converted to nitrate in the lake is as yet unknown and may involve past changes to the watershed or other anthropogenic factors.&lt;/p&gt;</gco:CharacterString>
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