http://lod.bco-dmo.org/id/dataset/3708
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2012-09-04
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Isotope ratios of carbon and nitrogen from harbor seals and prey species analyzed at the Stable Isotope Core Laboratory in Pullman, WA in 2009 (Seal_response_to_prey project)
2012-12-04
publication
2012-12-04
revision
BCO-DMO Linked Data URI
2012-12-04
creation
http://lod.bco-dmo.org/id/dataset/3708
Alejandro Acevedo-Gutierrez
Western Washington University
principalInvestigator
Jeffrey F Bromaghin
United States Geological Survey
principalInvestigator
Steven J Jeffries
Washington Department of Fish and Wildlife
principalInvestigator
John M Kennish
University of Alaska, Anchorage
principalInvestigator
Monique M Lance
Washington Department of Fish and Wildlife
principalInvestigator
Philip S. Levin
National Marine Fisheries Service
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Acevedo-Gutierrez, A., Levin, P. S., Lance, M. M., Jeffries, S. J., Bromaghin, J. F., Kennish, J. M. (2012) Isotope ratios of carbon and nitrogen from harbor seals and prey species analyzed at the Stable Isotope Core Laboratory in Pullman, WA in 2009 (Seal_response_to_prey project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 04 December 2012) Version Date 2012-12-04 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/3708 [access date]
Isotope ratios of carbon and nitrogen from harbor seals and prey species. Dataset Description: <p>In April and June 2009, tissues from harbor seals and their prey (fish species) were analyzed at the <a href="http://www.isotopes.wsu.edu/index.html" target="_blank">Stable Isotope Core Laboratory</a> at Washington State University. Reported results include: %C, %N, delta 13C relative to VPDB, and delta 15N relative to air.</p> Methods and Sampling: <p>Further methodology is available in thein the <a href="http://dmoserv3.whoi.edu/data_docs/Seal_Response_to_Prey/Stable_Isotopes_Report.pdf" target="_blank">lab report</a> (PDF).</p>
<p>Samples for isotopic analysis were converted to N2 and CO2 with an elemental analyzer (Costech Analytical ECS 4010). The two gases were separated with a 3m GC column and analyzed with a continuous flow isotope ratio mass spectrometer (Thermo Finnigan Delta PlusXP).</p>
<p>The 2-sigma uncertainty of both carbon and nitrogen isotopic results is 0.5 per mill, unless otherwise indicated.</p>
<p>Instrument stability checks and necessary calibrations were performed prior to all analyses. A linearity check prior to analysis of these samples indicated 13C linearity of 0.045 per mil/V and 15N linearity of 0.04 per mil per volt. These are within the 0.06 per mil/V spec defined by the manufacturer. Blind reference materials (in this case, two replicates of protein standard B-2155) were also analyzed with the samples as a check of normalization.<br />
<br />
Delta 13C and 15N values were calculated using a two point normalization by fitting a regression line through two running standards. %C and %N were calculated with a multi-point normalization using acetanilide. See the following:<br />
<a target="_blank" href="http://dmoserv3.whoi.edu/data_docs/Seal_Response_to_Prey/Stable_Isotopes_Normalization_0409.pdf">Normalization information for April 2009 samples</a> (PDF)<br />
<a target="_blank" href="http://dmoserv3.whoi.edu/data_docs/Seal_Response_to_Prey/Stable_Isotopes_Normalization_0609.pdf">Normalization information for June 2009 samples</a> (PDF)</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0550443 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0550443
completed
Alejandro Acevedo-Gutierrez
Western Washington University
1-360-650-3653
Department of Biology 516 High St. MS#9160
Bellingham
WA
98225-9160
USA
acevedo@biol.wwu.edu
pointOfContact
Jeffrey F Bromaghin
United States Geological Survey
1-907-786-7086
Alaska Science Center 4210 University Drive
Anchorage
AK
99508
USA
jbromaghin@usgs.gov
pointOfContact
Steven J Jeffries
Washington Department of Fish and Wildlife
1-253-589-7235
Marine Mammal Investigations 7801 Phillips Road SW
Lakewood
WA
98498
USA
Steven.Jeffries@dfw.wa.gov
pointOfContact
John M Kennish
University of Alaska, Anchorage
1-907-786-1236
Department of Chemistry and ASET Laboratory 3211 Providence Drive
Anchorage
AK
99508
USA
afjmk@uaa.alaska.edu
pointOfContact
Monique M Lance
Washington Department of Fish and Wildlife
1-253-691-3409
Wildlife Research Program 7801 Phillips Road SW
Lakewood
WA
98498
USA
Monique.Lance@dfw.wa.gov
pointOfContact
Philip S. Levin
National Marine Fisheries Service
206-860-3473
Northwest Fisheries Science Center, Northwest Regional Office 2725 Montlake Blvd E
Seattle
WA
98112
USA
Phil.Levin@noaa.gov
pointOfContact
asNeeded
Dataset Version: 04 December 2012
Unknown
common_name
lab_id
sample_id
d13C_VPDB
pcnt_C
d15N_air
pcnt_N
date_lab
CHN Elemental Analyzer
Isotope-ratio Mass Spectrometer
theme
None, User defined
common_name
sample identification
No BCO-DMO term
date
featureType
BCO-DMO Standard Parameters
CHN Elemental Analyzer
Isotope-ratio Mass Spectrometer
instrument
BCO-DMO Standard Instruments
Stable_Isotope_Core_seals
service
Deployment Activity
Pullman, WA
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Responses of Seals and Sea Lions to Increased Rockfish Density
http://biol.wwu.edu/mbel/?page=research
Responses of Seals and Sea Lions to Increased Rockfish Density
<p><strong>From NSF proposal:</strong><br />
This project is a collaborative study of the responses of harbor seals and other mammalian predators to changes in prey density in Puget Sound. The general study approach will involve multi-year field estimates to observe the responses of predators to rockfish density in protected areas, candidate marine reserves, and unprotected sites.</p>
<p>The collaborating investigators will estimate 1) rockfish density using visual and mark and recapture techniques; 2) predator abundance using aerials surveys and dedicated land observations; and 3) predator food consumption using scat to describe diet, tagging of harbor seals to describe individual foraging sites, and population-based and individual bioenergetics models to describe consumption of rockfish. The investigators will also take into account confounding factors that might explain predator behavior, such as environmental variables and alternative prey, by creating a GIS database from available information from the area. The different field observations and database estimates are explicitly linked through a common hypothesis and coordinated methodologies, and their results will be integrated into a model describing the impact of predation on rockfish populations. The responses of top predators to changes in prey density and their impact on fish populations of interest are unknown. This study will evaluate the effectiveness of MPAs as fish refugia, offer a framework for the management and conservation of marine resources, and provide an exciting opportunity for students to participate in ecological and conservation research.</p>
<p><strong>Hypotheses:</strong><br />
1) Harbor seals and other pinniped species show aggregative responses to changes in prey density. Hence, their abundance will increase with fish density.<br />
2) Harbor seals and other pinniped species show Type 2 or 3 functional responses to changes in prey density. Thus, their consumption rate of a particular prey type follows an asymptotic or sigmoidal curve relative to the prey’s density, respectively.<br />
3) Predation by harbor seals and other pinniped species is sufficiently intense that it impedes recovery of depleted fish populations.</p>
<p><strong>Objectives:</strong><br />
1) Quantify the number of harbor seals and other pinniped species in relation to rockfish density and other environmental (confounding) factors.<br />
2) Estimate the consumption rate of harbor seals and other pinniped species in relation to rockfish density and other prey species.<br />
3) Correlatively estimate the influence of predation by harbor seals and other pinniped species on survivorship and population size of rockfish.</p>
<p><strong>Publications resulting from this NSF award:</strong><br />
<strong>Bjorland</strong>, R. H., Pearson, S. F, Jeffries, S. J, Lance, M. M., Acevedo- Gutiérrez, A. & Ward, E. J. 2015. Stable isotope mixing models elucidate sex and size effects on the diet of a generalist marine predator. Marine Ecology Progress Series 526: 213-225. DOI: <a href="http://dx.doi.org/10.3354/meps11230" target="_blank">10.3354/meps11230</a><br />
<strong>Bromaghin</strong>, J. F., Lance, M. M., Elliott, E. W., Jeffries, S. J., Acevedo-Gutierrez, A. & Kennish, J. M. 2013. New insights into the diets of harbor seals in the Salish Sea of western North America revealed by quantitative fatty acid signature analysis. Fishery Bulletin 111: 13-26. DOI: <a href="http://dx.doi.org/10.7755/FB.111.1.2" target="_blank">10.7755/FB.111.1.2</a><br />
<strong>Buzzell</strong>, B.1, Lance, M. & Acevedo-Gutiérrez, A. 2014. Spatial and temporal variation in river otter (Lontra canadensis) diet and predation on rockfish (Genus Sebastes) in the San Juan Islands, Washington. Aquatic Mammals 40: 150- 161. DOI: <a href="http://dx.doi.org/10.1578/AM.40.2.2014.150" target="_blank">10.1578/AM.40.2.2014.150</a><br />
<strong>Howard</strong>, S., Lance, M., Jeffries, S. & Acevedo-Gutierrez, A. 2013. Fish consumption by harbor seals (Phoca vitulina) in the San Juan Islands, WA. Fishery Bulletin 111: 27-41. DOI: <a href="http://dx.doi.org/10.7755/FB.111.1.3" target="_blank">10.7755/FB.111.1.3</a><br />
<strong>Lance</strong>, M. M., Chang, W.-Y., Jeffries, S. J., Pearson, S. F. & Acevedo-Gutierrez, A. 2012. Harbor seal diet in northern Puget Sound: implications for the recovery of depressed fish stocks. Marine Ecology Progress Series 464:257-271. DOI:<a href="http://dx.doi.org/10.3354/meps09880" target="_blank">10.3354/meps09880</a><br />
<strong>Luxa</strong>, K. & Acevedo-Gutierrez, A. 2013. Food habits of harbor seals (<em>Phoca vitulina</em>) in two estuaries in the central Salish Sea. Aquatic Mammals 39: 10- 22. DOI: <a href="http://dx.doi.org/10.1578/AM.39.1.2013.10" target="_blank">10.1578/AM.39.1.2013.10</a><br />
<strong>Peterson</strong>, S., Lance, M. M., Jeffries, S. J. & Acevedo-Gutierrez, A. 2012. Long distance movements and disjunct spatial use of harbor seals (<em>Phoca vitulina</em>) in the inland waters of the Pacific Northwest. PLoS ONE 7: e39046. DOI: <a href="http://dx.doi.org/10.1371/journal.pone.0039046" target="_blank">10.1371/journal.pone.0039046</a><br />
<strong>Thomas</strong>, AC; Lance, MM; Jeffries, SJ; Miner, BG; Acevedo-Gutierrez, A. 2011. Harbor seal foraging response to a seasonal resource pulse, spawning Pacific herring. Marine Ecology-Progress Series, v.441. p. 225. DOI: <a href="http://dx.doi.org/10.3354/meps09370" target="_blank">10.3354/meps09370</a><br />
<strong>Ward</strong>, EJ; Levin, PS; Lance, MM; Jeffries, SJ; Acevedo-Gutierrez, A. 2012. Integrating diet and movement data to identify hot spots of predation risk and areas of conservation concern for endangered species. Conservation Letters, v.5, p. 37. DOI: <a href="http://dx.doi.org/10.1111/j.1755-263X.2011.00210.x" target="_blank">10.1111/j.1755-263X.2011.00210.x</a><br />
<strong>Wilson</strong>, K.2, Lance, M., Jeffries, S. & Acevedo-Gutiérrez, A. 2014. Fine-scale variability in harbor seal foraging behavior. PLoS ONE 9: e92838. DOI: <a href="http://dx.doi.org/10.1371/journal.pone.0092838" target="_blank">10.1371/journal.pone.0092838</a>.</p>
Seal_response_to_prey
largerWorkCitation
project
eng; USA
oceans
Pullman, WA
2012-12-04
Salish Sea, USA and Canada
0
BCO-DMO catalogue of parameters from Isotope ratios of carbon and nitrogen from harbor seals and prey species analyzed at the Stable Isotope Core Laboratory in Pullman, WA in 2009 (Seal_response_to_prey project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/29346.rdf
Name: common_name
Units: text
Description: <p>Common name of the species from which the sample was taken.</p>
http://lod.bco-dmo.org/id/dataset-parameter/29347.rdf
Name: lab_id
Units: unitless
Description: <p>Sample ID number assigned by the lab.</p>
http://lod.bco-dmo.org/id/dataset-parameter/29348.rdf
Name: sample_id
Units: unitless
Description: <p>Sample ID number.</p>
http://lod.bco-dmo.org/id/dataset-parameter/29349.rdf
Name: d13C_VPDB
Units: per mil
Description: <p>delta 13C relative to VPDB x 1000; the stable isotope ratio of 13C.</p>
http://lod.bco-dmo.org/id/dataset-parameter/29350.rdf
Name: pcnt_C
Units: %
Description: <p>Percent Carbon.</p>
http://lod.bco-dmo.org/id/dataset-parameter/29351.rdf
Name: d15N_air
Units: per mil
Description: <p>delta 15N relative to air x 1000; the stable isotope ratio of 15N.</p>
http://lod.bco-dmo.org/id/dataset-parameter/29352.rdf
Name: pcnt_N
Units: %
Description: <p>Percent Nitrogen.</p>
http://lod.bco-dmo.org/id/dataset-parameter/31213.rdf
Name: date_lab
Units: mm/dd/YYYY
Description: <p>Date of results reported by the lab.</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
23897
https://datadocs.bco-dmo.org/file/6YYMwZVtyw0gB3/seal_and_prey_stable_isotopes.csv
seal_and_prey_stable_isotopes.csv
Primary data file for dataset ID 3708
download
https://osprey.bco-dmo.org/dataset/3708/data/download
download
onLine
dataset
<p>Further methodology is available in thein the <a href="http://dmoserv3.whoi.edu/data_docs/Seal_Response_to_Prey/Stable_Isotopes_Report.pdf" target="_blank">lab report</a> (PDF).</p>
<p>Samples for isotopic analysis were converted to N2 and CO2 with an elemental analyzer (Costech Analytical ECS 4010). The two gases were separated with a 3m GC column and analyzed with a continuous flow isotope ratio mass spectrometer (Thermo Finnigan Delta PlusXP).</p>
<p>The 2-sigma uncertainty of both carbon and nitrogen isotopic results is 0.5 per mill, unless otherwise indicated.</p>
<p>Instrument stability checks and necessary calibrations were performed prior to all analyses. A linearity check prior to analysis of these samples indicated 13C linearity of 0.045 per mil/V and 15N linearity of 0.04 per mil per volt. These are within the 0.06 per mil/V spec defined by the manufacturer. Blind reference materials (in this case, two replicates of protein standard B-2155) were also analyzed with the samples as a check of normalization.<br />
<br />
Delta 13C and 15N values were calculated using a two point normalization by fitting a regression line through two running standards. %C and %N were calculated with a multi-point normalization using acetanilide. See the following:<br />
<a target="_blank" href="http://dmoserv3.whoi.edu/data_docs/Seal_Response_to_Prey/Stable_Isotopes_Normalization_0409.pdf">Normalization information for April 2009 samples</a> (PDF)<br />
<a target="_blank" href="http://dmoserv3.whoi.edu/data_docs/Seal_Response_to_Prey/Stable_Isotopes_Normalization_0609.pdf">Normalization information for June 2009 samples</a> (PDF)</p>
Specified by the Principal Investigator(s)
<p>Parameter names were modified to conform with BCO-DMO conventions. Data from 2 Excel files (1 for each processing date) were merged into 1 dataset.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
CHN Elemental Analyzer
CHN Elemental Analyzer
PI Supplied Instrument Name: CHN Elemental Analyzer PI Supplied Instrument Description:Samples were converted to N2 and CO2 with a Costech Analytical ECS 4010 elemental analyzer. Instrument Name: CHN Elemental Analyzer Instrument Short Name:CHN_EA Instrument Description: A CHN Elemental Analyzer is used for the determination of carbon, hydrogen, and nitrogen content in organic and other types of materials, including solids, liquids, volatile, and viscous samples.
Isotope-ratio Mass Spectrometer
Isotope-ratio Mass Spectrometer
PI Supplied Instrument Name: Isotope-ratio Mass Spectrometer PI Supplied Instrument Description:Samples were analyzed with a Thermo Finnigan Delta PlusXP continuous flow isotope ratio mass spectrometer. Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
Deployment: Stable_Isotope_Core_seals
Stable_Isotope_Core_seals
Stable Isotope Core Laboratory
Stable Isotope Core Laboratory
laboratory
Stable_Isotope_Core_seals
Alejandro Acevedo-Gutierrez
Western Washington University
Stable Isotope Core Laboratory
Stable Isotope Core Laboratory
laboratory