<div><p><b>Sampling and Analytical Methodology:</b><br />
Healthy and diseased sea fans were collected from Florida and Puerto Rico. <i> Labyrinthulomycetes</i> cells were isolated from a diseased sea fan from Florida; DNA extracted, amplified, cloned, sequenced, and deposited with accession # JQ248602. Fragments of healthy and diseased sea fans were extracted, amplified, cloned and sequenced under accession numbers JQ248603 (Florida), JQ248604 (Florida), JQ248605 (Puerto Rico), and JQ248606 (Puerto Rico).</p>
<p>Labyrinthulomycota small-subunit ribosomal DNA (SSU rRNA) specific primers, Laby-A and Laby-Y, were used (Stokes et al., 2002). Those containing bands of the expected (~ 430 bp) size were purified using the QIAquick PCR kit (Qiagen,) and sent to the Cornell University Life Sciences Core Laboratories for direct sequencing on an Applied Biosystems Automated 3730 DNA Analyzer. Although direct sequencing of all of the PCR products showed unambiguous DNA chromatographs throughout, the homogeneity of samples was additionally confirmed by cloning the PCR products into the TOPO-TA vector, using the TOPO TA cloning kit (Invitrogen) for sequencing of 2 to 5 clones of each isolate.</p></div>
Accession numbers for Labyrinthulomycetes detected in sea fans
<div><p>Accession numbers to NCBI's GenBank are provided for <i>Labyrinthulomycetes</i> detected in sea fans collected in Florida and Puerto Rico. <b></b></p>
<p>References:<br />
Burge CA, Douglas N, Conti-Jerpe I, Weil E, Roberts S, Friedman CS & CD Harvell. In press (May 2012) Friend or foe: the association of Labyrinthulomycetes with the Caribbean sea fan, <i>Gorgonia ventalina</i>. Dis Aquat Org.</p></div>
sea_fan_parasite_sequences
<div><p>The BLAST algorithm (Altschul et al.1990) was used to compare resulting sequences (overlapping 398 nt from Florida samples and 292 nt from Puerto Rico samples) with those deposited in the National Center for Biotechnology Information (NCBI) GenBank database, and alignments were made using the EMBL-EBI Clustal W tool (Larkin et al. 2007, Goujan et al. 2010). Representative sequences from the cultured microorganism and sea fan isolates were submitted to GenBank.</p></div>
3718
sea_fan_parasite_sequences
2012-09-12T13:26:49-04:00
2012-09-12T13:26:49-04:00
2023-07-07T16:10:26-04:00
urn:bcodmo:dataset:3718
Accession numbers for Labyrinthulomycetes detected in sea fans collected in the Florida Keys and Puerto Rico from 2006-2010 (Climate_CoralDisease project)
false
Harvell, D., Mydlarz, L. (2012) Accession numbers for Labyrinthulomycetes detected in sea fans collected in the Florida Keys and Puerto Rico from 2006-2010 (Climate_CoralDisease project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 12 Sept 2012) Version Date 2012-09-12 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/3718 [access date]
true
12 Sept 2012
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2012-09-12
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