http://lod.bco-dmo.org/id/dataset/3719
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2012-09-12
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Experimental results on the growth of Aplanochytrium (a sea fan parasite) cells over a temperature gradient conducted at the Harvell lab at Cornell University
2012-09-12
publication
2012-09-12
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-03-01
publication
https://doi.org/10.1575/1912/bco-dmo.3719.1
Drew Harvell
Cornell University
principalInvestigator
Laura Mydlarz
University of Texas at Arlington
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Harvell, D., Mydlarz, L. (2012) Experimental results on the growth of Aplanochytrium (a sea fan parasite) cells over a temperature gradient conducted at the Harvell lab at Cornell University. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2012-09-12 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.3719.1 [access date]
Growth of Aplanochytrium (a sea fan parasite) cells over a temperature gradient Dataset Description: <p>Two types of assays were used in two trials to quantify <em>Labyrinthulomycota</em> cultures: cell counts using a hemocytometer and total protein concentration.</p> Methods and Sampling: <p><strong>Sampling and Analytical Methodology:</strong><br />
In the first trial, a <em>Labyrinthulomycota</em> culture held at 22 degrees C was divided into 18 sub-cultures that were incubated at 15 degrees C, 20 degrees C, 25 degrees C, 30 degrees C, and 32 degrees C in triplicate for three days. Culture temperatures and incubation period were based on previous visual observations of <em>Labyrinthulomycota</em> growth, where over-growth of culture flasks occurs after three days at temperatures of 25 degrees C and higher. In the second trial, nine sub-cultures were incubated at 20 degrees C, 25 degrees C, and 30 degrees C in triplicate for three days.</p>
<p>In the second trial, total protein was also assessed. After three days, the media was poured off, rinsed once with 3 mLs of 0.22 um-filtered artificial sea water, and replaced with 3 mLs of 0.22 um-filtered artificial sea water. With a sterile wooden dowel, the bottom of each culture was scraped until no <em>Labyrinthulomycota</em> growth was visible on the bottom of the culture. 700 uL of each culture was placed in a bead beater and mixed at 300 rpm for 30 sec; 400 uL was set aside for protein assays and 300 uL for cell counts and held on ice until use.</p>
<p>Prior to counting using a hemocytometer, cells were vortexed for about 20 sec. In each culture, cells were counted in triplicate.</p>
<p>Total protein was extracted from each sample by adding 400 uL of extraction buffer (0.15 ug mL-1 DTT in Tris-HCl) to each tube. The contents of the tube were mixed and lysed for 2 minutes with the Fisherbrand disposable pestle grinder system, and incubated for 45 minutes on ice for extractions. Protein was measured using the DC protein kit (Bio-Rad), and read in triplicate using the Synergy HT multi-Detection microplate reader with KC4 software (Biotek Instruments, Vermont) at 750 nm.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0849776 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0849776
completed
Drew Harvell
Cornell University
607-254-4274
373 Pine Tree Road E321 Corson Hall
Ithaca
NY
14853
United States
cdh5@cornell.edu
pointOfContact
Laura Mydlarz
University of Texas at Arlington
817-272-0397
501 S. Nedderman Dr. 337 Life Sciences Building
Arlington
TX
76013
United States
mydlarz@uta.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
trial
temp
count_avg
count_avg_se
count_log10_avg
count_log10_avg_se
protein_avg
protein_avg_se
protein_log10_avg
protein_log10_avg_se
rep
count
protein
count_log10
protein_log10
theme
None, User defined
experiment id
No BCO-DMO term
count
featureType
BCO-DMO Standard Parameters
lab_Harvell
service
Deployment Activity
Cornell University Lab
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Influence of Temperature and Acidification on the Dynamics of Coral Co-Infection and Resistance
https://osprey.bco-dmo.org/project/2232
Influence of Temperature and Acidification on the Dynamics of Coral Co-Infection and Resistance
<p>This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).</p>
<p>Coral reef ecosystems are highly endangered by recent increases in temperature and by projected increases in ocean acidification. Although temperature has been identified as a driver of some coral disease outbreaks, nothing is known about direct effects of acidification on host immunity and pathogen virulence, or the potential for synergism with temperature. Natural coral populations often suffer from simultaneous infection by multiple pathogens that can also influence host immune responses, but co-infection dynamics have not been investigated in invertebrate systems lacking classical adaptive immunity. Changing climate will very likely influence the outcome of single and co-infection.</p>
<p>This project will investigate the influence of environmental stress on co-infection dynamics of the sea fan coral, <i>Gorgonia ventalina</i>, with a fungal pathogen, <i>Aspergillus sydowii</i> and a protist parasite, SPX. The goal is to identify the mechanisms through which multiple infections, temperature and acidification modify host resistance, leading to changes in within- and among-colony rates of disease spread.</p>
<p>The objectives of this project are to:<br />
(1) Identify incidence and co-infection frequency of <i>Aspergillus sydowii</i> and SPX. Detailed field surveys of the two diseases will test the hypothesis that co-infection is significant, provide valuable information about drivers of aspergillosis, and will help to characterize an emerging new sea fan disease.<br />
(2) Investigate how co-infection influences sea fan susceptibility, resistance, and within host disease dynamics. Through manipulative lab inoculation experiments we will test the hypothesis that single infections increase susceptibility to a second pathogen.<br />
(3) Examine the effects of temperature increase and ocean acidification on pathogen virulence, on underlying host resistance, and on the dynamics of single and co-infections.</p>
<p>The hypotheses that acidification will increase pathogen virulence and host susceptibility will be tested in a temperature and pH controlled experimental system. This system will also allow the potential synergistic effects of temperature and acidification on host immunity and co-infection dynamics to be explored. The primary intellectual merit of the proposed work will be a greater understanding of how changing climate mediates co-infection and immunity in a non-model invertebrate. While fungal pathogens are primarily opportunistic, labyrinthulid protozoans are recognized as primary pathogens in shellfish. Even in shellfish, little is known about co-infections involving labyrinthulids, and these protists are entirely unstudied in corals.</p>
<p><b>Publications associated with this project:</b><br />
Burge CA, Douglas N, Conti-Jerpe I, Weil E, Roberts S, Friedman CS & CD Harvell. (May 2012) Friend or foe: the association of<i> Labyrinthulomycetes</i> with the Caribbean sea fan,<i> Gorgonia ventalina</i>. Dis Aquat Org. 101:1-12. doi: <a href="http://dx.doi.org/10.3354/dao02487" target="_blank">10.3354/dao02487</a></p>
<p>Burge CA, Mouchka, ME, Harvell, CD & S Roberts. (In review) Immune response of the Caribbean sea fan, <i>Gorgonia ventalina</i> exposed to an <i>Aplanochytrium</i> parasite as revealed by transcriptome sequencing.</p>
Climate_CoralDisease
largerWorkCitation
project
eng; USA
biota
oceans
Cornell University Lab
2012-09-12
Florida Keys & Puerto Rico
0
BCO-DMO catalogue of parameters from Experimental results on the growth of Aplanochytrium (a sea fan parasite) cells over a temperature gradient conducted at the Harvell lab at Cornell University
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/29562.rdf
Name: trial
Units: unitless
Description: <p>Experimental trial number (1 or 2; see Acquisition Description).</p>
http://lod.bco-dmo.org/id/dataset-parameter/29563.rdf
Name: temp
Units: degrees C
Description: <p>Incubation temperature.</p>
http://lod.bco-dmo.org/id/dataset-parameter/29564.rdf
Name: count_avg
Units: # cells (unitless)
Description: <p>Average of total cells per temperature.</p>
http://lod.bco-dmo.org/id/dataset-parameter/29565.rdf
Name: count_avg_se
Units: # cells (unitless)
Description: <p>Standard error of count_avg.</p>
http://lod.bco-dmo.org/id/dataset-parameter/29566.rdf
Name: count_log10_avg
Units: # cells (unitless)
Description: <p>Average of log 10 transformed cell counts per temperature.</p>
http://lod.bco-dmo.org/id/dataset-parameter/29567.rdf
Name: count_log10_avg_se
Units: # cells (unitless)
Description: <p>Standard error of count_log10_avg.</p>
http://lod.bco-dmo.org/id/dataset-parameter/29568.rdf
Name: protein_avg
Units: mg protein per culture
Description: <p>Average protein concentration per temperature.</p>
http://lod.bco-dmo.org/id/dataset-parameter/29569.rdf
Name: protein_avg_se
Units: mg protein per culture
Description: <p>Standard error of protein_avg.</p>
http://lod.bco-dmo.org/id/dataset-parameter/29570.rdf
Name: protein_log10_avg
Units: mg protein per culture
Description: <p>Average of log 10 transformed protein concentration per temperature.</p>
http://lod.bco-dmo.org/id/dataset-parameter/29571.rdf
Name: protein_log10_avg_se
Units: mg protein per culture
Description: <p>Standard error of protein_log10_avg.</p>
http://lod.bco-dmo.org/id/dataset-parameter/29572.rdf
Name: rep
Units: unitless
Description: <p>Replicate.</p>
http://lod.bco-dmo.org/id/dataset-parameter/29573.rdf
Name: count
Units: # cells (unitless)
Description: <p>Total number of cells per culture.</p>
http://lod.bco-dmo.org/id/dataset-parameter/29574.rdf
Name: protein
Units: mg protein per culture
Description: <p>Protein concentration measured in milligrams of protein per culture.</p>
http://lod.bco-dmo.org/id/dataset-parameter/29575.rdf
Name: count_log10
Units: # cells (unitless)
Description: <p>Total cells per culture, log 10 transformed.</p>
http://lod.bco-dmo.org/id/dataset-parameter/29576.rdf
Name: protein_log10
Units: mg protein per culture
Description: <p>Protein concentration, log 10 transformed.</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
7249
https://darchive.mblwhoilibrary.org/bitstream/1912/23747/1/dataset-3719_seafanparasitegrowth__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.3719.1
download
onLine
dataset
<p><strong>Sampling and Analytical Methodology:</strong><br />
In the first trial, a <em>Labyrinthulomycota</em> culture held at 22 degrees C was divided into 18 sub-cultures that were incubated at 15 degrees C, 20 degrees C, 25 degrees C, 30 degrees C, and 32 degrees C in triplicate for three days. Culture temperatures and incubation period were based on previous visual observations of <em>Labyrinthulomycota</em> growth, where over-growth of culture flasks occurs after three days at temperatures of 25 degrees C and higher. In the second trial, nine sub-cultures were incubated at 20 degrees C, 25 degrees C, and 30 degrees C in triplicate for three days.</p>
<p>In the second trial, total protein was also assessed. After three days, the media was poured off, rinsed once with 3 mLs of 0.22 um-filtered artificial sea water, and replaced with 3 mLs of 0.22 um-filtered artificial sea water. With a sterile wooden dowel, the bottom of each culture was scraped until no <em>Labyrinthulomycota</em> growth was visible on the bottom of the culture. 700 uL of each culture was placed in a bead beater and mixed at 300 rpm for 30 sec; 400 uL was set aside for protein assays and 300 uL for cell counts and held on ice until use.</p>
<p>Prior to counting using a hemocytometer, cells were vortexed for about 20 sec. In each culture, cells were counted in triplicate.</p>
<p>Total protein was extracted from each sample by adding 400 uL of extraction buffer (0.15 ug mL-1 DTT in Tris-HCl) to each tube. The contents of the tube were mixed and lysed for 2 minutes with the Fisherbrand disposable pestle grinder system, and incubated for 45 minutes on ice for extractions. Protein was measured using the DC protein kit (Bio-Rad), and read in triplicate using the Synergy HT multi-Detection microplate reader with KC4 software (Biotek Instruments, Vermont) at 750 nm.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Deployment: lab_Harvell
lab_Harvell
Cornell
Cornell
laboratory
lab_Harvell
Drew Harvell
Cornell University
Cornell
Cornell
laboratory