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            <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/dataset/3749.rdf" xlink:actuate="onRequest">CTD cast sheets from R/V Thomas G. Thompson and R/V Kilo Moana cruises TN277, KM1301, and KM1312 in the Eastern North Pacific Ocean from 2012-2013 (POWOW project)</gmx:Anchor>
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                <gmx:Anchor xlink:href="https://ror.org/00py81415" xlink:title="ROR ID" xlink:actuate="onRequest">Duke University</gmx:Anchor>
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            <gco:CharacterString>Cite this dataset as: Johnson, Z. I. (2014) CTD cast sheets from R/V Thomas G. Thompson and R/V Kilo Moana cruises TN277, KM1301, and KM1312 in the Eastern North Pacific Ocean from 2012-2013 (POWOW project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2014-05-23 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/3749 [access date]</gco:CharacterString>
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        <gco:CharacterString>CTD cast sheets from POWOW cruises. Dataset Description: &amp;lt;p&amp;gt;CTD cast sheets (PDF) with basic information regarding when/where the CTD rosette was deployed and the niskin bottles that were sampled for various measurements. Cast sheets are provided from the POWOW1 (TN277), POWOW2 (KM1301) and POWOW3 (KM1312) cruises.&amp;lt;/p&amp;gt; Methods and Sampling: &amp;lt;p&amp;gt;Each station number is a new location. Cast numbers start at 1 for each station. CTD numbers are unique and sequential across stations/casts. Niskin bottles were sampled in the order listed on the cast sheet, from left to right (e.g. pH/DIC were sampled first, etc.).&amp;lt;/p&amp;gt;</gco:CharacterString>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/55018.rdf" xlink:title="OCE-1031064" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1031064 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1031064</gmx:Anchor>
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          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/deployment/58867.rdf" xlink:title="Cruise" xlink:actuate="onRequest">TN277</gmx:Anchor>
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                  <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/project/2237.rdf" xlink:title="Project" xlink:actuate="onRequest">Seasonal and decadal changes in temperature drive Prochlorococcus ecotype distribution patterns</gmx:Anchor>
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                            <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/project/2237.rdf" xlink:title="Project Name" xlink:actuate="onRequest">Seasonal and decadal changes in temperature drive Prochlorococcus ecotype distribution patterns</gmx:Anchor>
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                            <gco:CharacterString>&lt;p&gt;Project also known as '&lt;em&gt;Prochlorococcus&lt;/em&gt; Of Warming Ocean Waters' (POWOW).&lt;/p&gt;
&lt;p&gt;The two numerically-dominant ecotypes of the marine cyanobacterium &lt;em&gt;Prochlorococcus&lt;/em&gt; partition the surface ocean niche latitudinally, with ecotype eMIT9312 dominant in the 30 degree N to 30 degree S region and eMED4 dominant at higher latitudes. These ecotypes may account for 25-50% of primary production in open ocean ecosystems, but this percentage is dependent on which ecotype dominates. The relative abundance of the two ecotypes follows a log-linear relationship with temperature, with the transition from eMIT9312 to eMED4 occurring at approx. 18 degrees C. From these descriptive data, it has been hypothesized that temperature is the primary driver of relative abundance. Their contribution to net primary production, however, appears to be independent of temperature, suggesting temperature regulates ecotype dominance through photosynthesis-independent mechanisms.&lt;/p&gt;
&lt;p&gt;To test these hypotheses, the PIs are undertaking a series of field and lab studies to investigate the effect of temperature change on the distribution of these ecotypes. Two cruises in the North Pacific will trace the transitions from eMIT9312- to eMED4-dominated regions, with one cruise during the winter and the other during summer. They have hypothesized that the ratio of ecotype abundance will move latitudinally with the seasonal shift in temperature gradient: migration of the 18 degrees C isotherm northward in the summer will be matched by a similar migration of the 1:1 ecotype transition point. Multiple crossings of the 18 degrees C isotherm are proposed, and the summer cruise will also follow the isotherm to the Western US coast to gain insight on physical and geochemical influences. Environmental variables such as nutrient concentrations, light/mixing depths, and virus /grazing based mortality, which may impinge on the relationship between temperature and ecotype ratio, will be assessed through a series of multivariate analyses of the collected suite of physical, chemical and biological data. Seasonal comparisons will be complemented with on-deck incubations and lab competition assays (using existing and new isolates) that will establish, for the first time, how fitness coefficients of these ecotypes relate to temperature. As latitudinal shifts in temperature gradient and migration of ecotypes during seasonal warming likely share common features with high latitude warming as a consequence of climate change, the investigator's analyses will contribute important biological parameters (e.g., abundances, production rates, temperature change coefficients) for modeling biological and biogeochemical responses to climate change. This research will be integrated with that of committed collaborators, generating data sufficient for ecosystem-scale characterizations of the contributions of temperature (relative to other forcing factors) in constraining the range and seasonal migration of these numerically dominant marine phototrophs.&lt;/p&gt;
&lt;p&gt;&lt;strong&gt;Publications produced as result of this research:&lt;/strong&gt;&lt;br /&gt;
Rowe, J.M., DeBruyn, J.M., Poorvin, L., LeCleir, G.R., Johnson, Z.I., Zinser, E.R., and Wilhelm, S.W. 2012. Viral and bacterial abundance and production in the Western Pacific Ocean and the relation to other oceanic realms. FEMS Microbiology Ecology, 72, p. 359. DOI: &lt;a href=&quot;http://dx.doi.org/10.1111/j.1574-6941.2011.01223.x&quot; target=&quot;_blank&quot;&gt;10.1111/j.1574-6941.2011.01223.x&lt;/a&gt;&lt;/p&gt;
&lt;p&gt;Morris, J.J., Lenski, R.E. and E.R. Zinser. 2012. The Black Queen Hypothesis: Evolution of Dependencies through Adaptive Gene Loss. mBio, 3, p. e00036-12. DOI: &lt;a href=&quot;http://dx.doi.org/10.1128/mBio.00036-12&quot; target=&quot;_blank&quot;&gt;10.1128/mBio.00036-12&lt;/a&gt;&lt;/p&gt;
&lt;p&gt;Morris, J.J., Johnson, Z.I., Szul, M.J., Keller, M., and Zinser, E.R. 2011. Dependence of the cyanobacterium &lt;em&gt;Prochlorococcus&lt;/em&gt; on hydrogen peroxide scavenging microbes for growth at the ocean's surface. PLoS One, 6(2), p. 16805. DOI:&lt;a href=&quot;http://dx.doi.org/10.1371/journal.pone.0016805&quot; target=&quot;_blank&quot;&gt;10.1371/journal.pone.0016805&lt;/a&gt;&lt;/p&gt;
&lt;p&gt;Ringuet, S., Sassano, L., and Johnson, Z.I. 2011. A suite of microplate reader-based colorimetric methods to quantify ammonium, nitrate, orthophosphate and silicate concentrations for aquatic nutrient monitoring. Journal of Environmental Monitoring. DOI:&lt;a href=&quot;http://dx.doi.org/10.1039/C0EM00290A&quot; target=&quot;_blank&quot;&gt;10.1039/C0EM00290A&lt;/a&gt;&lt;/p&gt;
&lt;p&gt;Ritchie, A.E. and Johnson, Z.I. 2012. Abundance and genetic diversity of aerobic anoxygenic phototrophic bacteria of coastal regions of the Pacific Ocean. Applied and Environmental Microbiology, 78, p. 2858. DOI: &lt;a href=&quot;http://dx.doi.org/10.1128/AEM.06268-11&quot; target=&quot;_blank&quot;&gt;10.1128/AEM.06268-11&lt;/a&gt;&lt;/p&gt;</gco:CharacterString>
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