http://lod.bco-dmo.org/id/dataset/3751
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2012-10-22
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Extracted chlorophyll data from CTD rosette bottles from R/V Thomas G. Thompson and R/V Kilo Moana cruises TN277, KM1301, KM1312 in the Eastern North Pacific Ocean from 2012-2013 (POWOW project)
2014-07-16
publication
2014-07-16
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-03-05
publication
https://doi.org/10.1575/1912/bco-dmo.3751.3
Zackary I. Johnson
Duke University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Johnson, Z. (2014) Extracted chlorophyll data from CTD rosette bottles from R/V Thomas G. Thompson and R/V Kilo Moana cruises TN277, KM1301, KM1312 in the Eastern North Pacific Ocean from 2012-2013 (POWOW project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 3) Version Date 2014-07-16 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.3751.3 [access date]
Extracted chlorophyll data from CTD rosette bottles from 3 POWOW cruises. Dataset Description: <p>Extracted chlorophyll concentrations from POWOW1 (TN277), POWOW2 (KM1301), and POWOW3 (KM1312) cruises. POWOW1 data include total chlorophyll (&gt;0.22 um), &gt;2 um, and &gt;20 um. POWOW2 and POWOW3 data include total chlorophyll (&gt;0.22 um) and &gt;0.8 um.</p> Methods and Sampling: <p><strong>Methods described in Johnson et al. 2010:</strong><br />
Chlorophyll concentrations were measured by filtering 100 mL of seawater sample onto a polycarbonate filter (of pore size 0.22 um, 2 um or 20 um on POWOW1; and of pore size 0.22 um or 0.8&nbsp;um on POWOW2) using gentle vacuum (&lt;100 mm Hg) and extracting in 100% MeOH at -20 degrees C in the dark for &gt;24 h following (Holm-Hansen and Riemann, 1978). Fluorescence was measured using a Turner Designs 10-AU fluorometer following Welschmeyer (1994) that was calibrated against a standard chlorophyll solution (Ritchie, 2006).</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1031064 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1031064
completed
Zackary I. Johnson
Duke University
1-252-504-7543
Marine Sciences and Conservation 135 Duke Marine Lab Rd.
Beaufort
NC
28516
USA
zij@duke.edu
pointOfContact
asNeeded
Dataset Version: 3
Unknown
cruise_name
cast
lat
lon
depth_w
month_utc
day_utc
year
time_utc
ISO_DateTime_UTC
depth
bot
chl_tot
chl_2um
chl_20um
chl_0d8um
Turner Designs Fluorometer -10-AU
theme
None, User defined
cruise name
cast
latitude
longitude
depth_w
month_utc
day_utc
year
time_utc
ISO_DateTime_UTC
depth
bottle number
chlorophyll a
No BCO-DMO term
chl_a_20u
featureType
BCO-DMO Standard Parameters
Turner Designs Fluorometer 10-AU
instrument
BCO-DMO Standard Instruments
TN277
KM1301
KM1312
service
Deployment Activity
Eastern North Pacific Ocean
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Seasonal and decadal changes in temperature drive Prochlorococcus ecotype distribution patterns
http://oceanography.ml.duke.edu/johnson/research/powow/
Seasonal and decadal changes in temperature drive Prochlorococcus ecotype distribution patterns
<p>Project also known as '<em>Prochlorococcus</em> Of Warming Ocean Waters' (POWOW).</p>
<p>The two numerically-dominant ecotypes of the marine cyanobacterium <em>Prochlorococcus</em> partition the surface ocean niche latitudinally, with ecotype eMIT9312 dominant in the 30 degree N to 30 degree S region and eMED4 dominant at higher latitudes. These ecotypes may account for 25-50% of primary production in open ocean ecosystems, but this percentage is dependent on which ecotype dominates. The relative abundance of the two ecotypes follows a log-linear relationship with temperature, with the transition from eMIT9312 to eMED4 occurring at approx. 18 degrees C. From these descriptive data, it has been hypothesized that temperature is the primary driver of relative abundance. Their contribution to net primary production, however, appears to be independent of temperature, suggesting temperature regulates ecotype dominance through photosynthesis-independent mechanisms.</p>
<p>To test these hypotheses, the PIs are undertaking a series of field and lab studies to investigate the effect of temperature change on the distribution of these ecotypes. Two cruises in the North Pacific will trace the transitions from eMIT9312- to eMED4-dominated regions, with one cruise during the winter and the other during summer. They have hypothesized that the ratio of ecotype abundance will move latitudinally with the seasonal shift in temperature gradient: migration of the 18 degrees C isotherm northward in the summer will be matched by a similar migration of the 1:1 ecotype transition point. Multiple crossings of the 18 degrees C isotherm are proposed, and the summer cruise will also follow the isotherm to the Western US coast to gain insight on physical and geochemical influences. Environmental variables such as nutrient concentrations, light/mixing depths, and virus /grazing based mortality, which may impinge on the relationship between temperature and ecotype ratio, will be assessed through a series of multivariate analyses of the collected suite of physical, chemical and biological data. Seasonal comparisons will be complemented with on-deck incubations and lab competition assays (using existing and new isolates) that will establish, for the first time, how fitness coefficients of these ecotypes relate to temperature. As latitudinal shifts in temperature gradient and migration of ecotypes during seasonal warming likely share common features with high latitude warming as a consequence of climate change, the investigator's analyses will contribute important biological parameters (e.g., abundances, production rates, temperature change coefficients) for modeling biological and biogeochemical responses to climate change. This research will be integrated with that of committed collaborators, generating data sufficient for ecosystem-scale characterizations of the contributions of temperature (relative to other forcing factors) in constraining the range and seasonal migration of these numerically dominant marine phototrophs.</p>
<p><strong>Publications produced as result of this research:</strong><br />
Rowe, J.M., DeBruyn, J.M., Poorvin, L., LeCleir, G.R., Johnson, Z.I., Zinser, E.R., and Wilhelm, S.W. 2012. Viral and bacterial abundance and production in the Western Pacific Ocean and the relation to other oceanic realms. FEMS Microbiology Ecology, 72, p. 359. DOI: <a href="http://dx.doi.org/10.1111/j.1574-6941.2011.01223.x" target="_blank">10.1111/j.1574-6941.2011.01223.x</a></p>
<p>Morris, J.J., Lenski, R.E. and E.R. Zinser. 2012. The Black Queen Hypothesis: Evolution of Dependencies through Adaptive Gene Loss. mBio, 3, p. e00036-12. DOI: <a href="http://dx.doi.org/10.1128/mBio.00036-12" target="_blank">10.1128/mBio.00036-12</a></p>
<p>Morris, J.J., Johnson, Z.I., Szul, M.J., Keller, M., and Zinser, E.R. 2011. Dependence of the cyanobacterium <em>Prochlorococcus</em> on hydrogen peroxide scavenging microbes for growth at the ocean's surface. PLoS One, 6(2), p. 16805. DOI:<a href="http://dx.doi.org/10.1371/journal.pone.0016805" target="_blank">10.1371/journal.pone.0016805</a></p>
<p>Ringuet, S., Sassano, L., and Johnson, Z.I. 2011. A suite of microplate reader-based colorimetric methods to quantify ammonium, nitrate, orthophosphate and silicate concentrations for aquatic nutrient monitoring. Journal of Environmental Monitoring. DOI:<a href="http://dx.doi.org/10.1039/C0EM00290A" target="_blank">10.1039/C0EM00290A</a></p>
<p>Ritchie, A.E. and Johnson, Z.I. 2012. Abundance and genetic diversity of aerobic anoxygenic phototrophic bacteria of coastal regions of the Pacific Ocean. Applied and Environmental Microbiology, 78, p. 2858. DOI: <a href="http://dx.doi.org/10.1128/AEM.06268-11" target="_blank">10.1128/AEM.06268-11</a></p>
POWOW
largerWorkCitation
project
eng; USA
oceans
Eastern North Pacific Ocean
-160.6166
-118.316
21.3911
46.6888
2012-03-01
2013-07-27
Eastern North Pacific Ocean
0
BCO-DMO catalogue of parameters from Extracted chlorophyll data from CTD rosette bottles from R/V Thomas G. Thompson and R/V Kilo Moana cruises TN277, KM1301, KM1312 in the Eastern North Pacific Ocean from 2012-2013 (POWOW project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/30146.rdf
Name: cruise_name
Units: text
Description: <p>Cruise identifier (POWOW1 = TN277 = R/V Thomas G. Thompson cruise 277; POWOW2 = KM1301 = R/V Kilo Moana cruise 1301).</p>
http://lod.bco-dmo.org/id/dataset-parameter/30147.rdf
Name: cast
Units: unitless
Description: <p>Consecutive CTD cast number. CTD numbers are unique and sequential across stations.</p>
http://lod.bco-dmo.org/id/dataset-parameter/30148.rdf
Name: lat
Units: decimal degrees
Description: <p>Latitude at start of CTD cast. Positive = North.</p>
http://lod.bco-dmo.org/id/dataset-parameter/30149.rdf
Name: lon
Units: decimal degrees
Description: <p>Longitude at start of CTD cast. Positive = East.</p>
http://lod.bco-dmo.org/id/dataset-parameter/30150.rdf
Name: depth_w
Units: meters
Description: <p>Depth of the water (bottom depth) at sampling station.</p>
http://lod.bco-dmo.org/id/dataset-parameter/30151.rdf
Name: month_utc
Units: mm (01 to 12)
Description: <p>2-digit month of year, UTC.</p>
http://lod.bco-dmo.org/id/dataset-parameter/30152.rdf
Name: day_utc
Units: dd (01 to 31)
Description: <p>2-digit day of month, UTC.</p>
http://lod.bco-dmo.org/id/dataset-parameter/30153.rdf
Name: year
Units: unitless
Description: <p>4-digit year. in YYYY format</p>
http://lod.bco-dmo.org/id/dataset-parameter/30154.rdf
Name: time_utc
Units: HHMM.mm
Description: <p>Time (UTC) at start of sample collection, 24-hour clock.</p>
http://lod.bco-dmo.org/id/dataset-parameter/30155.rdf
Name: ISO_DateTime_UTC
Units: YYYY-mm-ddTHH:MM:SS.ssZ
Description: <p>Date/Time (UTC) ISO8601 formatted. T indicates start of time string; Z indicates UTC.</p>
http://lod.bco-dmo.org/id/dataset-parameter/30156.rdf
Name: depth
Units: meters
Description: <p>Sample depth.</p>
http://lod.bco-dmo.org/id/dataset-parameter/30157.rdf
Name: bot
Units: unitless
Description: <p>Rosette position of the bottle.</p>
http://lod.bco-dmo.org/id/dataset-parameter/30158.rdf
Name: chl_tot
Units: mg/m^3
Description: <p>Total chlorophyll concentration, in milligrams Chl-a per cubic meter.<br />
Measured using polycarbonate filter with 0.22um pore size.</p>
http://lod.bco-dmo.org/id/dataset-parameter/30159.rdf
Name: chl_2um
Units: mg/m^3
Description: <p>> 2um chlorophyll concentration, measured in milligrams Chl-a per cubic meter.<br />
Measured on POWOW1 cruise only using polycarbonate filter with 2um pore size.</p>
http://lod.bco-dmo.org/id/dataset-parameter/30160.rdf
Name: chl_20um
Units: mg/m^3
Description: <p>> 20um chlorophyll concentration, measured in milligrams Chl-a per cubic meter.<br />
Measured on POWOW1 cruise only using polycarbonate filter with 20um pore size.</p>
http://lod.bco-dmo.org/id/dataset-parameter/514787.rdf
Name: chl_0d8um
Units: mg/m^3
Description: <p>> 0.8 um chlorophyll concentration, measured in milligrams Chl-a per cubic meter.<br />
Measured on POWOW2 cruise only using polycarbonate filter with 0.8um pore size.</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
132849
https://darchive.mblwhoilibrary.org/bitstream/1912/23766/1/dataset-3751_chlorophyll__v3.tsv
download
https://doi.org/10.1575/1912/bco-dmo.3751.3
download
onLine
dataset
<p><strong>Methods described in Johnson et al. 2010:</strong><br />
Chlorophyll concentrations were measured by filtering 100 mL of seawater sample onto a polycarbonate filter (of pore size 0.22 um, 2 um or 20 um on POWOW1; and of pore size 0.22 um or 0.8&nbsp;um on POWOW2) using gentle vacuum (&lt;100 mm Hg) and extracting in 100% MeOH at -20 degrees C in the dark for &gt;24 h following (Holm-Hansen and Riemann, 1978). Fluorescence was measured using a Turner Designs 10-AU fluorometer following Welschmeyer (1994) that was calibrated against a standard chlorophyll solution (Ritchie, 2006).</p>
Specified by the Principal Investigator(s)
<p>&gt; 0.22 um referred to as "total" or simply "chlorophyll".</p>
<p><strong>BCO-DMO edits made:</strong><br />
- Parameter names have been changed to conform to BCO-DMO conventions.<br />
- month_utc, day_utc, year, and time_utc were added, based on the original ISO_DateTime_UTC field.<br />
- Rosette bottle numbers were added from the CTD cast sheets.<br />
- Replaced 'NaN' and '-999' with 'nd' to indicate 'no data'.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Turner Designs Fluorometer -10-AU
Turner Designs Fluorometer -10-AU
PI Supplied Instrument Name: Turner Designs Fluorometer -10-AU PI Supplied Instrument Description:Fluorescence was measured using a Turner Designs 10-AU fluorometer following Welschmeyer (1994) that was calibrated against a standard chlorophyll solution (Ritchie, 2006). Instrument Name: Turner Designs Fluorometer 10-AU Instrument Short Name:Turner Fluorometer 10-AU Instrument Description: The Turner Designs 10-AU Field Fluorometer is used to measure Chlorophyll fluorescence. The 10AU Fluorometer can be set up for continuous-flow monitoring or discrete sample analyses. A variety of compounds can be measured using application-specific optical filters available from the manufacturer (read more from Turner Designs, turnerdesigns.com, Sunnyvale, CA, USA). Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0393/
Cruise: TN277
TN277
R/V Thomas G. Thompson
Community Standard Description
International Council for the Exploration of the Sea
R/V Thomas G. Thompson
vessel
TN277
Zackary I. Johnson
Duke University
http://dmoserv3.whoi.edu/data_docs/POWOW/POWOW1-cruise_report.pdf
Report describing TN277
Cruise: KM1301
KM1301
R/V Kilo Moana
Community Standard Description
International Council for the Exploration of the Sea
R/V Kilo Moana
vessel
KM1301
Zackary I. Johnson
Duke University
http://dmoserv3.whoi.edu/data_docs/POWOW/POWOW2-cruise_report.pdf
Report describing KM1301
Cruise: KM1312
KM1312
R/V Kilo Moana
Community Standard Description
International Council for the Exploration of the Sea
R/V Kilo Moana
vessel
KM1312
Zackary I. Johnson
Duke University
http://dmoserv3.whoi.edu/data_docs/POWOW/POWOW3-cruise_report.pdf
Report describing KM1312
R/V Thomas G. Thompson
Community Standard Description
International Council for the Exploration of the Sea
R/V Thomas G. Thompson
vessel
R/V Kilo Moana
Community Standard Description
International Council for the Exploration of the Sea
R/V Kilo Moana
vessel