http://lod.bco-dmo.org/id/dataset/3770
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2012-11-07
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Results from experiments on Attheya sp. growth, productivity, and trace metal use under varying pCO2 and B12 levels (PhytoTM_in_HighCO2 project)
2012-11-02
publication
2012-11-02
revision
BCO-DMO Linked Data URI
2012-11-02
creation
http://lod.bco-dmo.org/id/dataset/3770
Feixue Fu
University of Southern California
principalInvestigator
David A. Hutchins
University of Southern California
principalInvestigator
Sergio A. Sanudo-Wilhelmy
University of Southern California
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Fu, F., Hutchins, D. A., Sanudo-Wilhelmy, S. A. (2012) Results from experiments on Attheya sp. growth, productivity, and trace metal use under varying pCO2 and B12 levels (PhytoTM_in_HighCO2 project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 02 Nov 2012) Version Date 2012-11-02 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/3770 [access date]
Attheya sp. growth, productivity, and trace metal use experiments under varying pCO2 and B12 levels. Dataset Description: <p>Experimental data testing the effects of B12 and CO2 availability on specific growth rates, primary productivity, cellular trace metal quotas, and net use efficiencies (NUEs) of the subarctic diatom <i>Attheya sp.</i></p>
<p>Data and methods are described in:<br />
King, A, Sanudo-Wilhelmy, S.A., Leblanc, K., Hutchins, D.A., Fu, F-X. CO2 and vitamin B12 interactions determine bioactive trace metal requirements of a subarctic Pacific diatom. ISME J. 2011 August; 5(8): 1388&ndash;1396. <span class="doi">doi:&nbsp; <a target="pmc_ext" href="http://dx.crossref.org/10.1038%2Fismej.2010.211">10.1038/ismej.2010.211</a></span></p> Methods and Sampling: <p>All methods below are described in King et al. 2011.</p>
<p><b>Organism source and culture conditions</b><br />
Unialgal axenic <i>Attheya sp.</i> (CCMP207) stock cultures, isolated from the Bering Sea, were obtained from the Provasoli-Guillard Culture Collection of Marine Phytoplankton (Boothbay Harbor, ME) and were grown in microwave-sterilized media prepared from 0.2 mm-filtered natural seawater (collected using trace metal clean techniques). Media had Aquil concentrations of nitrate, phosphate and silicic acid with additions of 5 uM EDTA, 451 nM Fe, 80 nM Zn, 50 nM Co and no added Cd (Price et al., 1988/89). Stock cultures were incubated at 3 degrees C with a 12 h:12 h light&ndash;dark cycle and an incident photon flux density of 80 &micro;mol photons per square meter per second.</p>
<p><b>Experimental treatments/conditions</b><br />
Experimental treatments included trace metal clean vitamin B12 additions of 500 and 10 ng per liter (370 and 7 pM) for the B12-replete and B12-limited cultures, respectively. Vitamin and nutrient stocks were cleaned of trace metals before use by passing them through a column packed with Chelex-100 (Bio-Rad, Hercules, CA, USA; Price et al., 1988/89). For both B12 conditions, triplicate acid-washed polycarbonate bottles were equilibrated with commercially prepared air:CO2 mixtures at three different CO2 concentrations: 200, 370 and 670 ppm pCO2. In-line HEPA filters were used to avoid trace metal and bacterial contamination from the gas tanks or lines.</p>
<p>Trace metal clean semi-continuous culturing methods were used during acclimation periods and steady-state growth. Final sampling was carried out following 4 to 8 weeks of semi-continuous incubation after statistically invariant growth rates were recorded for at least three consecutive transfers.</p>
<p>Seawater medium pCO2 in the experimental bottles was calculated throughout the experiment using measurements of pH and total dissolved inorganic carbon according to Dickson and Goyet (1994). To ensure that CO2 levels remained constant during growth, the pH in each bottle was monitored daily using a microprocessor pH meter, calibrated with pH 4, 7 and 10 buffer solutions. Total dissolved inorganic carbon was measured via coulometry (model CM 140, UIC, Joliet, IL, USA). The calculated pCO2 values (using CO2SYS; http://www.cdiac.ornl.gov/ftp/co2sys/CO2SYS_calc_XLS) from the final day of the experiment were 201&plusmn;13, 367&plusmn;21 and 671&plusmn;31 ppm (see table below); these treatments are referred to in the dataset and the literature using rounded-off values of 200, 370 and 670 ppm pCO2.</p>
<p><b>Seawater carbonate buffer system conditions (n=3):</b><br />
<table cellspacing="0" cellpadding="1" border="1">
<tbody>
<tr>
<td width="40%">Treatment</td>
<td width="20%">Measured pH (sd)</td>
<td width="20%">Measured DIC (sd); uM</td>
<td width="20%">Calculated pCO2 (sd); ppm</td>
</tr>
<tr>
<td>B12-limited, 208 p.p.m</td>
<td>8.36 (0.03)</td>
<td>1975 (27)</td>
<td>208 (15)</td>
</tr>
<tr>
<td>B12-limited, 380 p.p.m</td>
<td>8.13 (0.02)</td>
<td>2070 (20)</td>
<td>380 (18)</td>
</tr>
<tr>
<td>B12-limited, 680 p.p.m</td>
<td>7.91 (0.02)</td>
<td>2208 (13)</td>
<td>680 (32)</td>
</tr>
<tr>
<td>B12-replete, 195 p.p.m</td>
<td>8.38 (0.02)</td>
<td>1946 (16)</td>
<td>195 (10)</td>
</tr>
<tr>
<td>B12-replete, 353 p.p.m</td>
<td>8.15 (0.02)</td>
<td>2020 (8)</td>
<td>353 (16)</td>
</tr>
<tr>
<td>B12-replete, 661 p.p.m</td>
<td>7.92 (0.01)</td>
<td>2197 (12)</td>
<td>661 (31)</td>
</tr>
</tbody>
</table>
</p>
<p><b>Primary production and trace metal measurements</b><br />
Primary production was measured in triplicate using 24 h incubations with 3.7 kBq per mL H14CO3 under the appropriate experimental growth conditions of light and temperature for each treatment, followed by filtration and scintillation counting. Aliquots for analysis of particulate organic C and N were filtered onto pre-combusted 25mm GF/F filters (Whatman, Maidstone, UK) and analyzed using a 4010 CHNS Elemental Combustion System (Costech, Valencia, CA, USA). Samples for particulate organic phosphorous were determined spectrophotometrically ( Fu et al. (2005, 2007).</p>
<p>Particulate samples for trace metal analysis were filtered onto acid-washed 3-um-pore-size polycarbonate filters (Millipore, Billerica, MA, USA), rinsed with oxalate reagent to remove extracellular trace metals (Tovar-Sanchez et al., 2003), and Fe, Zn, Co and Cd were determined with a magnetic sectorfield high-resolution inductively coupled plasma mass spectrometer (ICPMS) (Element 2, Thermo, Waltham, MA, USA). Procedural filter blanks were also subjected to the same storage, digestion, dilution and analysis processes, and these blank values were subtracted from sample measurements (Sa&ntilde;udo-Wilhelmy et al., 2001). The digestion protocol consisted of sequential additions of ultrapure HCl, HNO3 and HF (Omnitrace Ultra; VWR, Westchester, PA, USA) and heating to 100 degrees C (Eggimann and Betzer, 1976).</p>
<p>The measured concentrations of particulate elements (C, N, Fe, Co, Zn and Cd) were normalized to P. Cellular elemental 'quota' or 'content' are used interchangeably with 'requirement'. Trace metal:C ratios were used in conjunction with specific growth rates to calculate net use efficiencies (NUEs; specific growth rate divided by trace metal:C ratio), representing how efficiently trace metals are used by cells for growth and C fixation (Raven, 1991).</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0850730 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0850730
completed
Feixue Fu
University of Southern California
2137403188
Department of Biology, MC 0371, University of Southern California 3616 Trousdale Pkwy
Los Angeles
CA
90089
USA
ffu@usc.edu
pointOfContact
David A. Hutchins
University of Southern California
213-740-5616
Department of Biological Sciences 3616 Trousdale Parkway, AHF 20
Los Angeles
CA
90089
USA
dahutch@usc.edu
pointOfContact
Sergio A. Sanudo-Wilhelmy
University of Southern California
213-821-1302
3616 Trousdale Pkwy Dept. of Biological Sciences, AHF 206, Mail Code: 0371
Los Angeles
CA
90089
USA
sanudo@usc.edu
pointOfContact
asNeeded
Dataset Version: 02 Nov 2012
Unknown
condition
pCO2
sp_growth_rate
sp_growth_rate_sd
pr_productivity
pr_productivity_sd
C_to_P
C_to_P_sd
N_to_P
N_to_P_sd
Fe_to_P
Fe_to_P_sd
Co_to_P
Co_to_P_sd
Zn_to_P
Zn_to_P_sd
Cd_to_P
Cd_to_P_sd
Fe_NUE
Fe_NUE_sd
Co_NUE
Co_NUE_sd
Zn_NUE
Zn_NUE_sd
Cd_NUE
Cd_NUE_sd
CHN Elemental Analyzer
Inductively Coupled Plasma Mass Spectrometer
theme
None, User defined
No BCO-DMO term
featureType
BCO-DMO Standard Parameters
CHN Elemental Analyzer
Inductively Coupled Plasma Mass Spectrometer
instrument
BCO-DMO Standard Instruments
lab_Fu
service
Deployment Activity
Los Angeles, CA
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Changing Phytoplankton Trace Metal Requirements in a High CO2 Ocean
https://osprey.bco-dmo.org/project/2241
Changing Phytoplankton Trace Metal Requirements in a High CO2 Ocean
<p>This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5). The award is also associated with the NSF Integrative Computing Education and Research (ICER) initiative.</p>
<p>Over the past two decades, the fundamental importance of iron and other bioactive trace metals in structuring marine food webs and biogeochemical cycles has been realized. Even more recently, over the past several years, the international ocean science community has begun to mobilize in an urgent effort to understand the ecosystem-level consequences of rising anthropogenic CO2 and acidification of the global ocean. This project examines the intersection of these two major research themes, by asking the question:<b> How will the trace element requirements of marine phytoplankton change in response to future increases in atmospheric pCO2?</b></p>
<p>Preliminary data generated by the investigators suggests that changing pCO2 can indeed profoundly affect the cellular quotas of Fe, Mo, Zn, Cd, Co and Mn in both prokaryotic and eukaryotic phytoplankton. Trace metals play critical roles as enzymatic co-factors for processes that are closely linked to the availability of CO2 such as carbon and nitrogen fixation, photosynthetic electron transport, and nutrient acquisition. Therefore, it is important to develop methods to quantitatively predict how algal metal requirements will change in tomorrow's rapidly changing ocean.</p>
<p>The investigators will take a three-pronged approach to addressing this overarching question:<br />
(1) Laboratory experiments will measure the trace metal quotas of steady-state cultures of key phytoplankton functional groups like diatoms, coccolithophores, Phaeocystis, and diazotrophic and pico-cyanobacteria while varying pCO2 both alone, and together with other limiting factors such as iron, temperature, and light.<br />
(2) Field work in the Southern California bight will provide measurements in trace metal stoichiometry of natural phytoplankton communities over a seasonal cycle in relation to pCO2 and other environmental variables -- this region is already experiencing some of the largest increases in acidic upwelled water along the entire West Coast.<br />
(3) This observational and correlative study will be coupled with manipulative experiments at the USC Catalina Island facility in which trace metal quotas of the same natural phytoplankton communities can be measured in relation to pCO2 shifts under controlled incubation conditions.</p>
<p>Together, these three complementary approaches will enable the investigators to determine over a variety of temporal and spatial scales how phytoplankton-driven trace element biogeochemistry is likely to change in a future high-CO2 ocean.</p>
PhytoTM_in_HighCO2
largerWorkCitation
project
eng; USA
oceans
Los Angeles, CA
2012-11-02
Laboratory
0
BCO-DMO catalogue of parameters from Results from experiments on Attheya sp. growth, productivity, and trace metal use under varying pCO2 and B12 levels (PhytoTM_in_HighCO2 project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/30500.rdf
Name: condition
Units: text
Description: <p>B12 treatment/condition. Replete = 370 pM B12; Limited = 7 pM B12.</p>
http://lod.bco-dmo.org/id/dataset-parameter/30501.rdf
Name: pCO2
Units: ppm CO2
Description: <p>Seawater pCO2 in the experimental bottles. Calculated using pH and total dissolved inorganic carbon (see Acquisition Description).</p>
http://lod.bco-dmo.org/id/dataset-parameter/30502.rdf
Name: sp_growth_rate
Units: unitless
Description: <p>Specific growth rate per day.</p>
http://lod.bco-dmo.org/id/dataset-parameter/30503.rdf
Name: sp_growth_rate_sd
Units: unitless
Description: <p>Standard deviation of sp_growth_rate.</p>
http://lod.bco-dmo.org/id/dataset-parameter/30504.rdf
Name: pr_productivity
Units: ug C / ug chl / h
Description: <p>14C based primary productivity in micrograms C per micorgrams chl per hour.</p>
http://lod.bco-dmo.org/id/dataset-parameter/30505.rdf
Name: pr_productivity_sd
Units: ug C / ug chl / h
Description: <p>Standard deviation of pr_productivity.</p>
http://lod.bco-dmo.org/id/dataset-parameter/30506.rdf
Name: C_to_P
Units: mol:mol
Description: <p>Carbon concentration normalized to Phosophorus.</p>
http://lod.bco-dmo.org/id/dataset-parameter/30507.rdf
Name: C_to_P_sd
Units: mol:mol
Description: <p>Standard deviation of C_to_P.</p>
http://lod.bco-dmo.org/id/dataset-parameter/30508.rdf
Name: N_to_P
Units: mol:mol
Description: <p>Nitrogen concentration normalized to Phosphorus.</p>
http://lod.bco-dmo.org/id/dataset-parameter/30509.rdf
Name: N_to_P_sd
Units: mol:mol
Description: <p>Standard deviation of N_to_P.</p>
http://lod.bco-dmo.org/id/dataset-parameter/30510.rdf
Name: Fe_to_P
Units: mmol:mol
Description: <p>Iron concentration normalized to Phosphorus.</p>
http://lod.bco-dmo.org/id/dataset-parameter/30511.rdf
Name: Fe_to_P_sd
Units: mmol:mol
Description: <p>Standard deviation of Fe_to_P.</p>
http://lod.bco-dmo.org/id/dataset-parameter/30512.rdf
Name: Co_to_P
Units: mmol:mol
Description: <p>Cobalt concentration normalized to Phosphorus.</p>
http://lod.bco-dmo.org/id/dataset-parameter/30513.rdf
Name: Co_to_P_sd
Units: mmol:mol
Description: <p>Standard deviation of Co_to_P.</p>
http://lod.bco-dmo.org/id/dataset-parameter/30514.rdf
Name: Zn_to_P
Units: mmol:mol
Description: <p>Zinc concentration normalized to Phosphorus.</p>
http://lod.bco-dmo.org/id/dataset-parameter/30515.rdf
Name: Zn_to_P_sd
Units: mmol:mol
Description: <p>Standard deviation of Zn_to_P.</p>
http://lod.bco-dmo.org/id/dataset-parameter/30516.rdf
Name: Cd_to_P
Units: mmol:mol
Description: <p>Cadmium concentration normalized to Phosphorus.</p>
http://lod.bco-dmo.org/id/dataset-parameter/30517.rdf
Name: Cd_to_P_sd
Units: mmol:mol
Description: <p>Standard deviation of Cd_to_P.</p>
http://lod.bco-dmo.org/id/dataset-parameter/30518.rdf
Name: Fe_NUE
Units: mol C fixed per mol Fe per day
Description: <p>Net use efficiency (NUE) of Iron. Specific growth rate divided by Fe:C ratio.</p>
http://lod.bco-dmo.org/id/dataset-parameter/30519.rdf
Name: Fe_NUE_sd
Units: mol C fixed per mol Fe per day
Description: <p>Standard deviation of Fe_NUE.</p>
http://lod.bco-dmo.org/id/dataset-parameter/30520.rdf
Name: Co_NUE
Units: mol C fixed per mol Co per day
Description: <p>Net use efficiency (NUE) of Cobalt. Specific growth rate divided by Co:C ratio.</p>
http://lod.bco-dmo.org/id/dataset-parameter/30521.rdf
Name: Co_NUE_sd
Units: mol C fixed per mol Co per day
Description: <p>Standard deviation of Co_NUE.</p>
http://lod.bco-dmo.org/id/dataset-parameter/30522.rdf
Name: Zn_NUE
Units: mol C fixed per mol Zn per day
Description: <p>Net use efficiency (NUE) of Zinc. Specific growth rate divided by Zn:C ratio.</p>
http://lod.bco-dmo.org/id/dataset-parameter/30523.rdf
Name: Zn_NUE_sd
Units: mol C fixed per mol Zn per day
Description: <p>Standard deviation of Zn_NUE.</p>
http://lod.bco-dmo.org/id/dataset-parameter/30524.rdf
Name: Cd_NUE
Units: mol C fixed per mol Cd per day
Description: <p>Net use efficiency (NUE) of Cadmium. Specific growth rate divided by Cd:C ratio.</p>
http://lod.bco-dmo.org/id/dataset-parameter/30525.rdf
Name: Cd_NUE_sd
Units: mol C fixed per mol Cd per day
Description: <p>Standard deviation of Cd_NUE.</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
1390
https://datadocs.bco-dmo.org/file/m77kzJ5i148ZRQ/Attheya_sp_exp.csv
Attheya_sp_exp.csv
Primary data file for dataset ID 3770
download
https://osprey.bco-dmo.org/dataset/3770/data/download
download
onLine
dataset
<p>All methods below are described in King et al. 2011.</p>
<p><b>Organism source and culture conditions</b><br />
Unialgal axenic <i>Attheya sp.</i> (CCMP207) stock cultures, isolated from the Bering Sea, were obtained from the Provasoli-Guillard Culture Collection of Marine Phytoplankton (Boothbay Harbor, ME) and were grown in microwave-sterilized media prepared from 0.2 mm-filtered natural seawater (collected using trace metal clean techniques). Media had Aquil concentrations of nitrate, phosphate and silicic acid with additions of 5 uM EDTA, 451 nM Fe, 80 nM Zn, 50 nM Co and no added Cd (Price et al., 1988/89). Stock cultures were incubated at 3 degrees C with a 12 h:12 h light&ndash;dark cycle and an incident photon flux density of 80 &micro;mol photons per square meter per second.</p>
<p><b>Experimental treatments/conditions</b><br />
Experimental treatments included trace metal clean vitamin B12 additions of 500 and 10 ng per liter (370 and 7 pM) for the B12-replete and B12-limited cultures, respectively. Vitamin and nutrient stocks were cleaned of trace metals before use by passing them through a column packed with Chelex-100 (Bio-Rad, Hercules, CA, USA; Price et al., 1988/89). For both B12 conditions, triplicate acid-washed polycarbonate bottles were equilibrated with commercially prepared air:CO2 mixtures at three different CO2 concentrations: 200, 370 and 670 ppm pCO2. In-line HEPA filters were used to avoid trace metal and bacterial contamination from the gas tanks or lines.</p>
<p>Trace metal clean semi-continuous culturing methods were used during acclimation periods and steady-state growth. Final sampling was carried out following 4 to 8 weeks of semi-continuous incubation after statistically invariant growth rates were recorded for at least three consecutive transfers.</p>
<p>Seawater medium pCO2 in the experimental bottles was calculated throughout the experiment using measurements of pH and total dissolved inorganic carbon according to Dickson and Goyet (1994). To ensure that CO2 levels remained constant during growth, the pH in each bottle was monitored daily using a microprocessor pH meter, calibrated with pH 4, 7 and 10 buffer solutions. Total dissolved inorganic carbon was measured via coulometry (model CM 140, UIC, Joliet, IL, USA). The calculated pCO2 values (using CO2SYS; http://www.cdiac.ornl.gov/ftp/co2sys/CO2SYS_calc_XLS) from the final day of the experiment were 201&plusmn;13, 367&plusmn;21 and 671&plusmn;31 ppm (see table below); these treatments are referred to in the dataset and the literature using rounded-off values of 200, 370 and 670 ppm pCO2.</p>
<p><b>Seawater carbonate buffer system conditions (n=3):</b><br />
<table cellspacing="0" cellpadding="1" border="1">
<tbody>
<tr>
<td width="40%">Treatment</td>
<td width="20%">Measured pH (sd)</td>
<td width="20%">Measured DIC (sd); uM</td>
<td width="20%">Calculated pCO2 (sd); ppm</td>
</tr>
<tr>
<td>B12-limited, 208 p.p.m</td>
<td>8.36 (0.03)</td>
<td>1975 (27)</td>
<td>208 (15)</td>
</tr>
<tr>
<td>B12-limited, 380 p.p.m</td>
<td>8.13 (0.02)</td>
<td>2070 (20)</td>
<td>380 (18)</td>
</tr>
<tr>
<td>B12-limited, 680 p.p.m</td>
<td>7.91 (0.02)</td>
<td>2208 (13)</td>
<td>680 (32)</td>
</tr>
<tr>
<td>B12-replete, 195 p.p.m</td>
<td>8.38 (0.02)</td>
<td>1946 (16)</td>
<td>195 (10)</td>
</tr>
<tr>
<td>B12-replete, 353 p.p.m</td>
<td>8.15 (0.02)</td>
<td>2020 (8)</td>
<td>353 (16)</td>
</tr>
<tr>
<td>B12-replete, 661 p.p.m</td>
<td>7.92 (0.01)</td>
<td>2197 (12)</td>
<td>661 (31)</td>
</tr>
</tbody>
</table>
</p>
<p><b>Primary production and trace metal measurements</b><br />
Primary production was measured in triplicate using 24 h incubations with 3.7 kBq per mL H14CO3 under the appropriate experimental growth conditions of light and temperature for each treatment, followed by filtration and scintillation counting. Aliquots for analysis of particulate organic C and N were filtered onto pre-combusted 25mm GF/F filters (Whatman, Maidstone, UK) and analyzed using a 4010 CHNS Elemental Combustion System (Costech, Valencia, CA, USA). Samples for particulate organic phosphorous were determined spectrophotometrically ( Fu et al. (2005, 2007).</p>
<p>Particulate samples for trace metal analysis were filtered onto acid-washed 3-um-pore-size polycarbonate filters (Millipore, Billerica, MA, USA), rinsed with oxalate reagent to remove extracellular trace metals (Tovar-Sanchez et al., 2003), and Fe, Zn, Co and Cd were determined with a magnetic sectorfield high-resolution inductively coupled plasma mass spectrometer (ICPMS) (Element 2, Thermo, Waltham, MA, USA). Procedural filter blanks were also subjected to the same storage, digestion, dilution and analysis processes, and these blank values were subtracted from sample measurements (Sa&ntilde;udo-Wilhelmy et al., 2001). The digestion protocol consisted of sequential additions of ultrapure HCl, HNO3 and HF (Omnitrace Ultra; VWR, Westchester, PA, USA) and heating to 100 degrees C (Eggimann and Betzer, 1976).</p>
<p>The measured concentrations of particulate elements (C, N, Fe, Co, Zn and Cd) were normalized to P. Cellular elemental 'quota' or 'content' are used interchangeably with 'requirement'. Trace metal:C ratios were used in conjunction with specific growth rates to calculate net use efficiencies (NUEs; specific growth rate divided by trace metal:C ratio), representing how efficiently trace metals are used by cells for growth and C fixation (Raven, 1991).</p>
Specified by the Principal Investigator(s)
<p>BCO-DMO merged data submitted as 4 separate tables into one dataset. Parameter names were modified to conform with BCO-DMO conventions.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
CHN Elemental Analyzer
CHN Elemental Analyzer
PI Supplied Instrument Name: CHN Elemental Analyzer PI Supplied Instrument Description:Particulate C and N were analyzed using a 4010 CHNS Elemental Combusion System. Instrument Name: CHN Elemental Analyzer Instrument Short Name:CHN_EA Instrument Description: A CHN Elemental Analyzer is used for the determination of carbon, hydrogen, and nitrogen content in organic and other types of materials, including solids, liquids, volatile, and viscous samples.
Inductively Coupled Plasma Mass Spectrometer
Inductively Coupled Plasma Mass Spectrometer
PI Supplied Instrument Name: Inductively Coupled Plasma Mass Spectrometer PI Supplied Instrument Description:Fe, Zn, Co, and Cd were determined with a magnetic sectorfield high-resolution inductively coupled plasma mass spectrometer (ICPMS) (Element 2, Thermo). Instrument Name: Inductively Coupled Plasma Mass Spectrometer Instrument Short Name:ICP Mass Spec Instrument Description: An ICP Mass Spec is an instrument that passes nebulized samples into an inductively-coupled gas plasma (8-10000 K) where they are atomized and ionized. Ions of specific mass-to-charge ratios are quantified in a quadrupole mass spectrometer. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB15/
Deployment: lab_Fu
lab_Fu
USC
laboratory
lab_Fu
Feixue Fu
University of Southern California
USC
laboratory