http://lod.bco-dmo.org/id/dataset/3778
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2012-11-14
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Experimental results from a study of growth rates of two strains of Trichodesmium erythraeum under varying pCO2 and light conditions (PhytoTM_in_HighCO2 project)
2012-11-14
publication
2012-11-14
revision
BCO-DMO Linked Data URI
2012-11-14
creation
http://lod.bco-dmo.org/id/dataset/3778
Feixue Fu
University of Southern California
principalInvestigator
David A. Hutchins
University of Southern California
principalInvestigator
Sergio A. Sanudo-Wilhelmy
University of Southern California
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Fu, F., Hutchins, D. A., Sanudo-Wilhelmy, S. A. (2012) Experimental results from a study of growth rates of two strains of Trichodesmium erythraeum under varying pCO2 and light conditions (PhytoTM_in_HighCO2 project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 14 Nov 2012) Version Date 2012-11-14 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/3778 [access date]
Growth rates of two strains of Trichodesmium erythraeum under varying pCO2 and light conditions. Dataset Description: <p>Experimental data on the growth rates of two strians of Trichodesmium erythraeum (IMS1010 and GBRTRLI10) in response to irradiance and pCO2. This experiment served as a reference for determining the range of irradiance levels used in the other CO2 and light experiments on T. erythraeum during this project.</p>
<p>Data and methods are described in:<br />
<b>Garcia</b>, N. S., Fu, F.-X., Breene, C. L., Bernhardt, P. W., Mulholland, M. R., Sohm, J. A. and Hutchins, D. A. (2011), INTERACTIVE EFFECTS OF IRRADIANCE AND CO2 ON CO2 FIXATION AND N2 FIXATION IN THE DIAZOTROPH TRICHODESMIUM ERYTHRAEUM (CYANOBACTERIA). Journal of Phycology, 47: 1292&ndash;1303. doi: <a href="http://dx.doi.org/10.1111/j.1529-8817.2011.01078.x" target="_blank">10.1111/j.1529-8817.2011.01078.x</a></p> Methods and Sampling: <p>The methods below are described in Garcia et al. (2011).</p>
<p><b>Culture conditions</b><br />
Stock and experimental cultures of <i>Trichodesmium erythraeum</i> GBRTRL101 (GBR; from the Great Barrier Reef, Pacific Ocean) and IMS101 (IMS; from coastal North Carolina, Atlantic Ocean) were cultured at 24 degrees C (unless otherwise stated) in an artificial seawater medium without fixed N using a modified version of the YBCII recipe of Chen et al. (1996). Phosphate and trace metal solutions were filtered (0.2 um) and added in concentrations equivalent to the AQUIL recipe (Morel et al. 1979) to microwave- (experiment with GBR) or autoclave-sterilized (experiments with IMS) seawater. Irradiance was supplied with cool white fluorescent bulbs on a 12:12 light:dark cycle. For all experiments, cultures were grown in triplicate using a semi-continuous batch culturing method to achieve steady state exponential growth for approximately 7-10 generations prior to sampling, in order to fully acclimatize cells to treatment pCO2 and irradiance conditions. We monitored cell density every 2-3 days using microscopic cell counts. When the biomass reached approximately 100-200 trichomes per mL (~100-200 nmol C per mL), we diluted cultures with fresh medium to 50-100 trichomes per mL (~50-100 nmol C per mL). In this semi-continuous culturing method, the growth rate determines the dilution rate; this culturing technique does not attempt to control the growth rate with the dilution rate, as continuous culturing methods do.</p>
<p><b>Experimental Design: </b><b>IMS growth vs. light experiment<br />
</b>This experiment served as a reference for determining the range of irradiance levels used in our CO2 and light experiments. Cultures of IMS were cultured at 27 degrees C in fifteen 800-mL culturing flasks on a 12:12 light:dark cycle at 25, 50, 100, 180 and 300 umol quanta per square meter per second irradiance. Once cells had achieved steady state, we determined cellular growth rates using microscopic cell counts between dilutions.</p>
<p><b>Analytical Methods:&nbsp; Growth rates</b><br />
Growth rates were estimated by measuring relative increases in cell number per unit volume between dilutions (2-3 day periods) in steady-state cultures.</p>
<p><b>Seawater carbonate system estimates</b><br />
Total dissolved inorganic carbon (DIC) was preserved in whole water samples (5-70 mL; stored at 4 degrees C) with a 5% HgCl2 solution (final concentration diluted to 0.5% HgCl2) as described in Fu et al. (2007), and estimated by acidifying 5 mL and quantifying the CO2 trapped in an acid sparging column (model: CM 5230) with a carbon coulometer (model: CM 140, UIC inc., Joliet, IL, USA). Reference material for the DIC analysis was prepared by Andrew Dickson at Scripps Institute of Oceanography. pH was measured with a pH meter (model: Orion 5 star Thermo Scientific, Beverly, MA, USA) and was monitored to ensure that perturbations of the seawater with the either air or certified pre-mixed air (Gilmore Liquid Air Company 750 ppm) resulted in the desired target pH of either ~8.2 or ~7.95. For the CO2/light manipulation experiment with GBR, samples for total DIC were taken from cultures at the same time CO2 and N2 fixation rates were estimated. For the CO2/light manipulation experiment with IMS, measurements of pH were paired with total DIC samples 5-6 days prior to measuring rates of CO2- and N2 fixation and were used to calculate pCO2 at 24 degrees C using the CO2sys program provided by Lewis and Wallace (1998) with K1 and K2 constants from Mehrbach et al. (1973) refit by Dickson and Millero (1987).</p>
<p><b>Carbonate system parameters in the experiment:</b><br />
<table cellspacing="0" cellpadding="2" border="1">
<tbody>
<tr>
<td>Strain</td>
<td>pCO2 treatment</td>
<td>DIC (uM)</td>
<td>pH</td>
<td>Calculated pCO2 (ppm)</td>
</tr>
<tr>
<td>IMS</td>
<td>Present day</td>
<td>2018 &plusmn;27</td>
<td>8.23 &plusmn;0.01</td>
<td>435 &plusmn; 9</td>
</tr>
<tr>
<td>IMS</td>
<td>100-year projected</td>
<td>2116 &plusmn;15</td>
<td>8.00 &plusmn; 0</td>
<td>771 &plusmn; 8</td>
</tr>
<tr>
<td>GBR</td>
<td>Present-day</td>
<td>2037 &plusmn;9</td>
<td>n.d.</td>
<td>n.d.</td>
</tr>
<tr>
<td>GBR</td>
<td>100-year projected</td>
<td>2165 &plusmn;9</td>
<td>n.d.</td>
<td>n.d.</td>
</tr>
</tbody>
</table>
</p>
<p><b>References:</b><b><br />
Chen</b>, Y. B., Zehr, J. P. &amp; Mellon, M. 1996. Growth and nitrogen fixation of the diazotrophic filamentous nonheterocystous cyanobacterium Trichodesmium sp IMS 101 in defined media: Evidence for a circadian rhythm. J. Phycol. 32:916-923. DOI:&nbsp;<a href="http://onlinelibrary.wiley.com/doi/10.1111/j.0022-3646.1996.00916.x/abstract" target="_blank">10.1111/j.0022-3646.1996.00916.x</a><br />
<b>Dickson</b>, A. G., &amp; Millero, F. J. 1987. A comparison of the equilibrium constants for the dissociation of carbonic acid in seawater media. Deep-Sea Res. 34:1733-1743. DOI: <a href="http://dx.doi.org/10.1016/0198-0149(87)90021-5" target="_blank">10.1016/0198-0149(87)90021-5</a><br />
<b>Lewis</b>, E. and D. W. R. Wallace (1998). Program Developed for CO2 System Calculations. ORNL/CDIAC-105. Carbon Dioxide Information Analysis Center, Oak Ridge National Laboratory, U.S. Department of Energy, Oak Ridge, Tenessee. Available at: <a target="_blank" href="http://cdiac.ornl.gov/oceans/co2rprt.html">http://cdiac.ornl.gov/oceans/co2rprt.html</a>.<br />
<b>Mehrbach</b>, Y., Culberson, C., Hawley, J. &amp; Pytkovicz, R. 1973. Measurement of the apparent dissociation constants of carbonic acid in seawater at atmospheric pressure. Limnol. Oceanogr. 18:897-907.<br />
<b>Morel</b>, F. M. M., Rueter, J. G., Anderson, D. M. and Guillard, R. R. L. 1979. Aquil - chemically defined phytoplankton culture-medium for trace-metal studies. J. Phycol. 15:135-141. DOI:&nbsp;<a target="_blank" href="http://dx.doi.org/10.1111/j.1529-8817.1979.tb02976.x">10.1111/j.1529-8817.1979.tb02976.x</a></p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0850730 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0850730
completed
Feixue Fu
University of Southern California
2137403188
Department of Biology, MC 0371, University of Southern California 3616 Trousdale Pkwy
Los Angeles
CA
90089
USA
ffu@usc.edu
pointOfContact
David A. Hutchins
University of Southern California
213-740-5616
Department of Biological Sciences 3616 Trousdale Parkway, AHF 20
Los Angeles
CA
90089
USA
dahutch@usc.edu
pointOfContact
Sergio A. Sanudo-Wilhelmy
University of Southern California
213-821-1302
3616 Trousdale Pkwy Dept. of Biological Sciences, AHF 206, Mail Code: 0371
Los Angeles
CA
90089
USA
sanudo@usc.edu
pointOfContact
asNeeded
Dataset Version: 14 Nov 2012
Unknown
strain
pCO2
irradiance
growth_rate
growth_rate_SD
Benchtop pH Meter
theme
None, User defined
taxon
No BCO-DMO term
irradiance
featureType
BCO-DMO Standard Parameters
Benchtop pH Meter
instrument
BCO-DMO Standard Instruments
lab_Fu
service
Deployment Activity
Los Angeles, CA
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Changing Phytoplankton Trace Metal Requirements in a High CO2 Ocean
https://osprey.bco-dmo.org/project/2241
Changing Phytoplankton Trace Metal Requirements in a High CO2 Ocean
<p>This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5). The award is also associated with the NSF Integrative Computing Education and Research (ICER) initiative.</p>
<p>Over the past two decades, the fundamental importance of iron and other bioactive trace metals in structuring marine food webs and biogeochemical cycles has been realized. Even more recently, over the past several years, the international ocean science community has begun to mobilize in an urgent effort to understand the ecosystem-level consequences of rising anthropogenic CO2 and acidification of the global ocean. This project examines the intersection of these two major research themes, by asking the question:<b> How will the trace element requirements of marine phytoplankton change in response to future increases in atmospheric pCO2?</b></p>
<p>Preliminary data generated by the investigators suggests that changing pCO2 can indeed profoundly affect the cellular quotas of Fe, Mo, Zn, Cd, Co and Mn in both prokaryotic and eukaryotic phytoplankton. Trace metals play critical roles as enzymatic co-factors for processes that are closely linked to the availability of CO2 such as carbon and nitrogen fixation, photosynthetic electron transport, and nutrient acquisition. Therefore, it is important to develop methods to quantitatively predict how algal metal requirements will change in tomorrow's rapidly changing ocean.</p>
<p>The investigators will take a three-pronged approach to addressing this overarching question:<br />
(1) Laboratory experiments will measure the trace metal quotas of steady-state cultures of key phytoplankton functional groups like diatoms, coccolithophores, Phaeocystis, and diazotrophic and pico-cyanobacteria while varying pCO2 both alone, and together with other limiting factors such as iron, temperature, and light.<br />
(2) Field work in the Southern California bight will provide measurements in trace metal stoichiometry of natural phytoplankton communities over a seasonal cycle in relation to pCO2 and other environmental variables -- this region is already experiencing some of the largest increases in acidic upwelled water along the entire West Coast.<br />
(3) This observational and correlative study will be coupled with manipulative experiments at the USC Catalina Island facility in which trace metal quotas of the same natural phytoplankton communities can be measured in relation to pCO2 shifts under controlled incubation conditions.</p>
<p>Together, these three complementary approaches will enable the investigators to determine over a variety of temporal and spatial scales how phytoplankton-driven trace element biogeochemistry is likely to change in a future high-CO2 ocean.</p>
PhytoTM_in_HighCO2
largerWorkCitation
project
eng; USA
oceans
Los Angeles, CA
2012-11-14
Laboratory
0
BCO-DMO catalogue of parameters from Experimental results from a study of growth rates of two strains of Trichodesmium erythraeum under varying pCO2 and light conditions (PhytoTM_in_HighCO2 project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/30739.rdf
Name: strain
Units: text
Description: <p>Name of the T. erythraeum strain. IMS101 = from Coastal North Carolina, Atlantic Ocean; GBRTRLI101 = from Great Barrier Reef, Pacific Ocean.</p>
http://lod.bco-dmo.org/id/dataset-parameter/30740.rdf
Name: pCO2
Units: ppm
Description: <p>Partial pressure of pCO2. Present-day levels = 380 ppm; 100-year predicted levels = 750 ppm.</p>
http://lod.bco-dmo.org/id/dataset-parameter/30741.rdf
Name: irradiance
Units: umol quanta m-2 s-1
Description: <p>Light/irradiance level measured in umol quanta per square meter per second.</p>
http://lod.bco-dmo.org/id/dataset-parameter/30742.rdf
Name: growth_rate
Units: cellular growth per day
Description: <p>Cellular growth rate. Estimated from changes in cell number per unit volume over time.</p>
http://lod.bco-dmo.org/id/dataset-parameter/30743.rdf
Name: growth_rate_SD
Units: cellular growth per day
Description: <p>Standard deviation of growth_rate based on the means of the triplicate samples.</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
355
https://datadocs.bco-dmo.org/file/jAABwDluYBRRW7/tricho_growth.csv
tricho_growth.csv
Primary data file for dataset ID 3778
download
https://osprey.bco-dmo.org/dataset/3778/data/download
download
onLine
dataset
<p>The methods below are described in Garcia et al. (2011).</p>
<p><b>Culture conditions</b><br />
Stock and experimental cultures of <i>Trichodesmium erythraeum</i> GBRTRL101 (GBR; from the Great Barrier Reef, Pacific Ocean) and IMS101 (IMS; from coastal North Carolina, Atlantic Ocean) were cultured at 24 degrees C (unless otherwise stated) in an artificial seawater medium without fixed N using a modified version of the YBCII recipe of Chen et al. (1996). Phosphate and trace metal solutions were filtered (0.2 um) and added in concentrations equivalent to the AQUIL recipe (Morel et al. 1979) to microwave- (experiment with GBR) or autoclave-sterilized (experiments with IMS) seawater. Irradiance was supplied with cool white fluorescent bulbs on a 12:12 light:dark cycle. For all experiments, cultures were grown in triplicate using a semi-continuous batch culturing method to achieve steady state exponential growth for approximately 7-10 generations prior to sampling, in order to fully acclimatize cells to treatment pCO2 and irradiance conditions. We monitored cell density every 2-3 days using microscopic cell counts. When the biomass reached approximately 100-200 trichomes per mL (~100-200 nmol C per mL), we diluted cultures with fresh medium to 50-100 trichomes per mL (~50-100 nmol C per mL). In this semi-continuous culturing method, the growth rate determines the dilution rate; this culturing technique does not attempt to control the growth rate with the dilution rate, as continuous culturing methods do.</p>
<p><b>Experimental Design: </b><b>IMS growth vs. light experiment<br />
</b>This experiment served as a reference for determining the range of irradiance levels used in our CO2 and light experiments. Cultures of IMS were cultured at 27 degrees C in fifteen 800-mL culturing flasks on a 12:12 light:dark cycle at 25, 50, 100, 180 and 300 umol quanta per square meter per second irradiance. Once cells had achieved steady state, we determined cellular growth rates using microscopic cell counts between dilutions.</p>
<p><b>Analytical Methods:&nbsp; Growth rates</b><br />
Growth rates were estimated by measuring relative increases in cell number per unit volume between dilutions (2-3 day periods) in steady-state cultures.</p>
<p><b>Seawater carbonate system estimates</b><br />
Total dissolved inorganic carbon (DIC) was preserved in whole water samples (5-70 mL; stored at 4 degrees C) with a 5% HgCl2 solution (final concentration diluted to 0.5% HgCl2) as described in Fu et al. (2007), and estimated by acidifying 5 mL and quantifying the CO2 trapped in an acid sparging column (model: CM 5230) with a carbon coulometer (model: CM 140, UIC inc., Joliet, IL, USA). Reference material for the DIC analysis was prepared by Andrew Dickson at Scripps Institute of Oceanography. pH was measured with a pH meter (model: Orion 5 star Thermo Scientific, Beverly, MA, USA) and was monitored to ensure that perturbations of the seawater with the either air or certified pre-mixed air (Gilmore Liquid Air Company 750 ppm) resulted in the desired target pH of either ~8.2 or ~7.95. For the CO2/light manipulation experiment with GBR, samples for total DIC were taken from cultures at the same time CO2 and N2 fixation rates were estimated. For the CO2/light manipulation experiment with IMS, measurements of pH were paired with total DIC samples 5-6 days prior to measuring rates of CO2- and N2 fixation and were used to calculate pCO2 at 24 degrees C using the CO2sys program provided by Lewis and Wallace (1998) with K1 and K2 constants from Mehrbach et al. (1973) refit by Dickson and Millero (1987).</p>
<p><b>Carbonate system parameters in the experiment:</b><br />
<table cellspacing="0" cellpadding="2" border="1">
<tbody>
<tr>
<td>Strain</td>
<td>pCO2 treatment</td>
<td>DIC (uM)</td>
<td>pH</td>
<td>Calculated pCO2 (ppm)</td>
</tr>
<tr>
<td>IMS</td>
<td>Present day</td>
<td>2018 &plusmn;27</td>
<td>8.23 &plusmn;0.01</td>
<td>435 &plusmn; 9</td>
</tr>
<tr>
<td>IMS</td>
<td>100-year projected</td>
<td>2116 &plusmn;15</td>
<td>8.00 &plusmn; 0</td>
<td>771 &plusmn; 8</td>
</tr>
<tr>
<td>GBR</td>
<td>Present-day</td>
<td>2037 &plusmn;9</td>
<td>n.d.</td>
<td>n.d.</td>
</tr>
<tr>
<td>GBR</td>
<td>100-year projected</td>
<td>2165 &plusmn;9</td>
<td>n.d.</td>
<td>n.d.</td>
</tr>
</tbody>
</table>
</p>
<p><b>References:</b><b><br />
Chen</b>, Y. B., Zehr, J. P. &amp; Mellon, M. 1996. Growth and nitrogen fixation of the diazotrophic filamentous nonheterocystous cyanobacterium Trichodesmium sp IMS 101 in defined media: Evidence for a circadian rhythm. J. Phycol. 32:916-923. DOI:&nbsp;<a href="http://onlinelibrary.wiley.com/doi/10.1111/j.0022-3646.1996.00916.x/abstract" target="_blank">10.1111/j.0022-3646.1996.00916.x</a><br />
<b>Dickson</b>, A. G., &amp; Millero, F. J. 1987. A comparison of the equilibrium constants for the dissociation of carbonic acid in seawater media. Deep-Sea Res. 34:1733-1743. DOI: <a href="http://dx.doi.org/10.1016/0198-0149(87)90021-5" target="_blank">10.1016/0198-0149(87)90021-5</a><br />
<b>Lewis</b>, E. and D. W. R. Wallace (1998). Program Developed for CO2 System Calculations. ORNL/CDIAC-105. Carbon Dioxide Information Analysis Center, Oak Ridge National Laboratory, U.S. Department of Energy, Oak Ridge, Tenessee. Available at: <a target="_blank" href="http://cdiac.ornl.gov/oceans/co2rprt.html">http://cdiac.ornl.gov/oceans/co2rprt.html</a>.<br />
<b>Mehrbach</b>, Y., Culberson, C., Hawley, J. &amp; Pytkovicz, R. 1973. Measurement of the apparent dissociation constants of carbonic acid in seawater at atmospheric pressure. Limnol. Oceanogr. 18:897-907.<br />
<b>Morel</b>, F. M. M., Rueter, J. G., Anderson, D. M. and Guillard, R. R. L. 1979. Aquil - chemically defined phytoplankton culture-medium for trace-metal studies. J. Phycol. 15:135-141. DOI:&nbsp;<a target="_blank" href="http://dx.doi.org/10.1111/j.1529-8817.1979.tb02976.x">10.1111/j.1529-8817.1979.tb02976.x</a></p>
Specified by the Principal Investigator(s)
<p>Parameter names have been changed to conform with BCO-DMO conventions.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Benchtop pH Meter
Benchtop pH Meter
PI Supplied Instrument Name: Benchtop pH Meter PI Supplied Instrument Description:pH was measured with an Orion 5 star Thermo Scientific (Beverly, MA, USA) pH meter. Instrument Name: Benchtop pH Meter Instrument Short Name:Benchtop pH Meter Instrument Description: An instrument consisting of an electronic voltmeter and pH-responsive electrode that gives a direct conversion of voltage differences to differences of pH at the measurement temperature. (McGraw-Hill Dictionary of Scientific and Technical Terms)
This instrument does not map to the NERC instrument vocabulary term for 'pH Sensor' which measures values in the water column. Benchtop models are typically employed for stationary lab applications.
Deployment: lab_Fu
lab_Fu
USC
laboratory
lab_Fu
Feixue Fu
University of Southern California
USC
laboratory