http://lod.bco-dmo.org/id/dataset/3813
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2012-12-04
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Competition experiments of pCO2-conditioned clones in artificial communities; conducted in the Hutchins Laboratory, University of Southern California from 2009-2010
2012-11-20
publication
2012-11-20
revision
BCO-DMO Linked Data URI
2012-11-20
creation
http://lod.bco-dmo.org/id/dataset/3813
David Caron
University of Southern California
principalInvestigator
Feixue Fu
University of Southern California
principalInvestigator
David A. Hutchins
University of Southern California
principalInvestigator
Astrid Schnetzer
University of Southern California
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Caron, D., Schnetzer, A., Fu, F., Hutchins, D. A. (2012) Competition experiments of pCO2-conditioned clones in artificial communities; conducted in the Hutchins Laboratory, University of Southern California from 2009-2010. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2012-11-20) Version Date 2012-11-20 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/3813 [access date]
Compete pCO2 conditioned clones in artificial communities Dataset Description: <p>The&nbsp;&ldquo;Switch&rdquo; competition experiments conditioned clones at each pCO2 were competed in artificial communities at two other pCO2 levels. This experiment was performed after 12-months.</p>
<p>A flow chart of the experimental design is shown in <a target="_blank" href="http://dmoserv3.whoi.edu/data_docs/Plankton_acclimation/Figure_1.gif"><font color="#006699">Figure 1</font></a>, including collection and incubation of the natural bloom in a short-term (2 week) pCO2 experiment, isolation of cells of all four species from each pCO2 treatment, conditioning of the isolates at the pCO2 from which they were isolated for one year, and recombining isolates into artificial communities to compete for 2-week periods following 4, 8 and 12 months of conditioning. Growth rates of each species were assessed during conditioning in unialgal cultures at the 8-month timepoint, as well as in mixed communities during the initial bloom experiment and the 12-month artificial community experiment. These data are available as a separate data set called Dinoflagellate Evo.</p> Methods and Sampling: <p><b>Clonal culture isolations</b></p>
<p>Three individual cells representing the four dominant genera were isolated from each incubation bottle at the end of the two-week incubation of the natural community, and maintained in long-term culture (52 weeks) at the pCO2 from which they were obtained under conditions of temperature, light, nutrients, CO2 bubbling, etc., identical to the 2-week natural community experiment. Cultures were maintained in exponential phase using autoclave-sterilized enriched seawater growth medium with semi-continuous weekly dilutions based on specific growth rates within each bottle, calculated as in Tatters et al. (2012). The approximate number of generations during this time period was: <i>L. polyedrum</i> (48-62), <i>P. micans</i> (58-71), <i>Alexandrium</i> sp. (34-38) and <i>Gonyaulax</i> sp. (75-126).</p>
<p><b>Artificial community competition experiments</b></p>
<p>The conditioned clonal cultures were recombined into artificial communities after 4, 8 and 12 months in the same relative proportions and cell densities as the original natural bloom assemblage. The 8- and 12-month experiments used triplicate communities of all four species, but due to logistical limitations, the 4-month experiment used only <i>L. polyedrum</i>, <i>P. micans</i>, and <i>Alexandrium</i> sp. in duplicate communities. Because <i>Gonyaulax</i> sp. was not included in this preliminary 4-month experiment and replication was different, it is not fully comparable to the other experiments. The dinoflagellates in the artificial community trials were allowed to compete under identical experimental conditions of light, temperature, nutrient availability, and pCO2 for the same time period and diluted exactly as in the original natural bloom incubation. Samples were collected for cell counts and carbonate system parameters (<a target="_blank" href="http://dmoserv3.whoi.edu/data_docs/Plankton_acclimation/Table_1.gif">Table 1</a>) in all experiments.</p>
<p><b>Switch experiment</b></p>
<p>A triplicated CO2 switch experiment at the 12-month time point consisted of &lsquo;switching&rsquo; low CO2-conditioned cell lines to medium and high CO2 (low --&gt; medium and low --&gt; high), medium CO2-conditioned cell lines to low and high CO2 (medium --&gt; low and medium --&gt; high), and high CO2-conditioned clones to low and medium CO2 (high --&gt; low and high --&gt; medium). The same experimental bottles employed in the 12-month artificial community experiments described above were used to provide appropriate controls of low --&gt; low, medium --&gt; medium, and high --&gt; high. Other than the switched pCO2 treatments, protocols for these trials were exactly the same as in the other artificial community experiments.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0962309 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0962309
completed
David Caron
University of Southern California
213-740-0203
Dornsife College of Letters, Arts, and Sciences 3616 Trousdale Parkway
Los Angeles
CA
90089
USA
dcaron@usc.edu
pointOfContact
Feixue Fu
University of Southern California
2137403188
Department of Biology, MC 0371, University of Southern California 3616 Trousdale Pkwy
Los Angeles
CA
90089
USA
ffu@usc.edu
pointOfContact
David A. Hutchins
University of Southern California
213-740-5616
Department of Biological Sciences 3616 Trousdale Parkway, AHF 20
Los Angeles
CA
90089
USA
dahutch@usc.edu
pointOfContact
Astrid Schnetzer
University of Southern California
919-515-7837
2800 Faucette Drive 4148 Jordan Hall
Raleigh
NC
27695
USA
aschnet@ncsu.edu
pointOfContact
asNeeded
Dataset Version: 2012-11-20
Unknown
CO2_partial_pressure
species
sample
growth_rate
count
theme
None, User defined
No BCO-DMO term
species scientific name (binomial)
count_species
featureType
BCO-DMO Standard Parameters
lab_Hutchins_09-10_plankton
service
Deployment Activity
Hutchins Laboratory, University of Southern California
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Experimental studies to understand and evaluate acclimation of marine plankton assemblages to increased CO2 and temperature
https://osprey.bco-dmo.org/project/2247
Experimental studies to understand and evaluate acclimation of marine plankton assemblages to increased CO2 and temperature
<p>Progressing ocean acidification and increasing sea surface temperature may significantly impact marine plankton community structure and community-level processes. Yet, our ability to predict specific responses is still limited because of the tremendous taxonomic complexity of microbial assemblages and the limitations of the methodological and experimental tools presently available to test specific hypotheses. Research to study community level effects due to a changing CO2/temperature regime often involve short-term field incubations that subject organisms to simulated 'greenhouse' conditions. A central question for understanding global climate change is whether the trends and patterns that are observed in communities during short-term manipulations can be extrapolated to the responses of fully acclimated plankton communities over decadal or longer timescales.</p>
<p>The specific objectives of this research program are: 1) to examine how protistan communities restructure in response to increased seawater CO2 concentrations and temperature in semi-continuous field incubation experiments, and 2) to evaluate if the dominant algal species that are isolated from either ambient or increased CO2 and temperature treatments in field experiments will re-establish dominance under the same conditions in acclimated laboratory culture competition studies. Changes in community structure of natural protistan assemblages in our experimental treatments will be followed using image-based methods (flow cytometry, FlowCAM and microscopy) in combination with state-of the art molecular tools (DNA fingerprinting). Molecular approaches have begun to reveal an incredible high diversity for marine microbes and stimulate debate in regard to the ubiquitous presence of a microbial 'Rare Biosphere' that is, the presence of a huge number of species that are present at extremely small percentages of the total abundance of microbes, among a much smaller percentage of dominant ones. Little is known about the ecological significance of these rare species, and the investigators hypothesize that change in CO2 and temperature will select for some of these members that are inconspicuous under ambient conditions.</p>
<p>The unique aspect of this experimental approach is the combined use of field incubations that encompass entire natural microbial assemblages, with a series of laboratory culture competition trials that focus on the same groups of algae after extended acclimation, to evaluate the validity of short-term experiments that examine changing CO2 and temperature. First, field incubation experiments will be conducted to characterize changes in protistan community structure under ambient and future CO2/temperature regimes. Second, clonal algal strains will be isolated from dominant taxa in present day and greenhouse treatments, and cultivated for extended periods under their 'preferred' CO2/temperature conditions. Finally, mixtures of these acclimated strains will be competed against each other, to re-examine their responses to ambient and greenhouse conditions and compare them to the responses observed in the unacclimated field incubation experiments.</p>
<p>Two graduate students will make this project the focus of their Ph.D. research at USC, and undergraduate students will be involved in the field and laboratory work. Results from this research will be incorporated in lesson plans on microbial diversity and global climate change. Dissemination of data and results is planned on a project website. The PIs in this project also participate in an on-going, innovative, NSF-funded program (Centers for Ocean Science Education Excellence; COSEE-West) which focuses on personal involvement of faculty in a custom framework to allow an effective connection with K-12 teachers, thus improving math and science education in disadvantaged parts of Southern California.</p>
<div id="nuan_ria_plugin">
<param name="tabId" value="ff-tab-1" />
<param name="counter" value="210" /></div>
Plankton acclimation
largerWorkCitation
project
eng; USA
oceans
Hutchins Laboratory, University of Southern California
2012-11-20
Bloom sample retrieved from near-shore water near Venice Beach, CA
0
BCO-DMO catalogue of parameters from Competition experiments of pCO2-conditioned clones in artificial communities; conducted in the Hutchins Laboratory, University of Southern California from 2009-2010
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/31214.rdf
Name: CO2_partial_pressure
Units: n/a
Description: <p>Start and end partial pressures</p>
http://lod.bco-dmo.org/id/dataset-parameter/31215.rdf
Name: species
Units: n/a
Description: <p>Species name</p>
http://lod.bco-dmo.org/id/dataset-parameter/31216.rdf
Name: sample
Units: n/a
Description: <p>Sample name, A, B, or C</p>
http://lod.bco-dmo.org/id/dataset-parameter/31217.rdf
Name: growth_rate
Units: divisions per day
Description: <p>Growth rate</p>
http://lod.bco-dmo.org/id/dataset-parameter/31218.rdf
Name: count
Units: cell counts per mL
Description: <p>Count of the cells</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2528
https://datadocs.bco-dmo.org/file/zqqA8Z2cRzMEN6/switch_competition.csv
switch_competition.csv
Primary data file for dataset ID 3813
download
https://osprey.bco-dmo.org/dataset/3813/data/download
download
onLine
dataset
<p><b>Clonal culture isolations</b></p>
<p>Three individual cells representing the four dominant genera were isolated from each incubation bottle at the end of the two-week incubation of the natural community, and maintained in long-term culture (52 weeks) at the pCO2 from which they were obtained under conditions of temperature, light, nutrients, CO2 bubbling, etc., identical to the 2-week natural community experiment. Cultures were maintained in exponential phase using autoclave-sterilized enriched seawater growth medium with semi-continuous weekly dilutions based on specific growth rates within each bottle, calculated as in Tatters et al. (2012). The approximate number of generations during this time period was: <i>L. polyedrum</i> (48-62), <i>P. micans</i> (58-71), <i>Alexandrium</i> sp. (34-38) and <i>Gonyaulax</i> sp. (75-126).</p>
<p><b>Artificial community competition experiments</b></p>
<p>The conditioned clonal cultures were recombined into artificial communities after 4, 8 and 12 months in the same relative proportions and cell densities as the original natural bloom assemblage. The 8- and 12-month experiments used triplicate communities of all four species, but due to logistical limitations, the 4-month experiment used only <i>L. polyedrum</i>, <i>P. micans</i>, and <i>Alexandrium</i> sp. in duplicate communities. Because <i>Gonyaulax</i> sp. was not included in this preliminary 4-month experiment and replication was different, it is not fully comparable to the other experiments. The dinoflagellates in the artificial community trials were allowed to compete under identical experimental conditions of light, temperature, nutrient availability, and pCO2 for the same time period and diluted exactly as in the original natural bloom incubation. Samples were collected for cell counts and carbonate system parameters (<a target="_blank" href="http://dmoserv3.whoi.edu/data_docs/Plankton_acclimation/Table_1.gif">Table 1</a>) in all experiments.</p>
<p><b>Switch experiment</b></p>
<p>A triplicated CO2 switch experiment at the 12-month time point consisted of &lsquo;switching&rsquo; low CO2-conditioned cell lines to medium and high CO2 (low --&gt; medium and low --&gt; high), medium CO2-conditioned cell lines to low and high CO2 (medium --&gt; low and medium --&gt; high), and high CO2-conditioned clones to low and medium CO2 (high --&gt; low and high --&gt; medium). The same experimental bottles employed in the 12-month artificial community experiments described above were used to provide appropriate controls of low --&gt; low, medium --&gt; medium, and high --&gt; high. Other than the switched pCO2 treatments, protocols for these trials were exactly the same as in the other artificial community experiments.</p>
Specified by the Principal Investigator(s)
<p>For the 12-month switch competition trials, we used final cell abundances from all treatments to examine the comparative effects of differing pCO2 competition levels and differing pCO2 conditioning levels. Using a two-way crossed design for the ANOSIM routine, we tested the average effect on overall community structure and on the four individual species separately of pCO2 levels during competition removing differences in conditioning pCO2, and the average effect of conditioning pCO2 removing differences in competition pCO2 (Clarke and Warwick, 2001).</p>
<p><b>Literature Cited</b></p>
<div>Clark, K. R., and R. M. Warwick. 2001. Change in marine communities: an approach to statistical analysis and interpretation. 2<sup>nd</sup> edition. PRIMER-E, UK.</div>
<p>&nbsp;</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Deployment: lab_Hutchins_09-10_plankton
lab_Hutchins_09-10_plankton
USC
laboratory
lab_Hutchins_09-10_plankton
David Caron
University of Southern California
USC
laboratory