<div><p>Sampling and Analytical Methodology: <br />
Sampling was conducted monthly from May 2008-April 2010 at the Bermuda Atlantic Time-Series (BATS). Samples were collected for DNA analysis from four depths in the upper water column and from 150 m particle traps. DNA was extracted from these samples and a region of the 18S ribosomal RNA gene for eukaryotes and 16S rRNA gene for cyanobacteria was amplified by PCR using eukaryotic and cyanobacterial primers. Each amplified sample was subjected to Denaturing Gradient Gel Electrophoresis in order to “fingerprint” the community. Individual bands were excised and identified by sequence matching using the NCBI database (<a href="http://blast.ncbi.nlm.nih.gov">http://blast.ncbi.nlm.nih.gov</a>). Quantity One software package was used to determine similarity between samples. The efficiency of recovery was determined by qPCR in order to accurately calculate the DNA present in the water column and DNA flux into particle traps. For further detail please refer to:</p>
<p>Jessica Amacher, Susanne Neuer, Michael Lomas. 2013. DNA-based molecular fingerprinting of eukaryotic protists and cyanobacteria contributing to sinking particle flux at the Bermuda Atlantic time-series study. Deep Sea Research part II. 93: 71-83.</p></div>
BATS phytoplankton and cyanobacteria DGGE DNA data
<div><p>This dataset includes eukaryotic and cyanobacterial DNA sequence data obtained from the upper water column as well as from shallow drifting traps during regular BATS cruises from May 2008-April 2010. Specifically, it contains a complete list of denaturing gradient gel results and the closest sequence matches from the NCBI database for each excised band (phylotype) as well as original denaturing gradient gel images.<br />
</p></div>
Phytoflux_BATS
<div><p>Data Processing:<br />
Denaturing gradient gels were imaged and analyzed with the BioRad Fluor-S imager. Bands were selected and matched manually for band matrices included in these data.</p>
<p>BCO-DMO Note: Cruise 242 was on the RV/Oceanus, not the Atlantic Explorer. The cruise id was corrected 5/13/2013. (NJC)</p></div>
3818
Phytoflux_BATS
2012-12-14T10:47:23-05:00
2012-12-14T10:47:23-05:00
2023-07-07T16:10:26-04:00
urn:bcodmo:dataset:3818
BATS phytoplankton and cyanobacteria DGGE DNA data from R/V Atlantic Explorer, R/V Oceanus cruises in the Bermuda Atlantic Time Series Station (BATS) from 2008-2010 (Plankton particle flux project)
false
Neuer, S., Lomas, M. W. (2013) BATS phytoplankton and cyanobacteria DGGE DNA data from R/V Atlantic Explorer, R/V Oceanus cruises in the Bermuda Atlantic Time Series Station (BATS) from 2008-2010 (Plankton particle flux project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2013-04-15) Version Date 2013-04-15 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/3818 [access date]
true
2013-04-15
false
2013-04-15
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