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See Howard et al. 2010 and Rinta-Kanto et al. 2011 for detailed methods, summarized below:<\/p>\n
\"In October 2006, seawater was collected from surface waters (<1 m deep) in the Gulf of Mexico off the coast of Dauphin Island, AL (lat: 30 03.041N; lon: 87 59.708W). Water was filtered through a 200-um mesh into six 20-liter polyethylene Cubitainers with minimal headspace.<\/p>\n
Three microcosms were amended with 10 um sodium nitrate (NaNO3) and 0.6 um potassium phosphate (K2HPO4) to serve as the experimental microcosms. Three microcosms were left untreated to serve as the control. The Cubitainers were maintained at 27 degrees C on a 12-hour light\/dark cycle for the duration of the experiment.<\/p>\n
Fv:Fm ratios were measured using a Walz Water PAM (pulse amplitude modulation) fluorometer.\"<\/p>\n
Experimental design is further described in:<\/strong> Fv:Fm ratios from control and experimental microcosms from the Dauphin Island Cubitainer Experiment (DICE) measured using a Pulse Amplitude Modulation (PAM) fluorometer.<\/p><\/div>","@type":"rdf:HTML"}],"http:\/\/www.w3.org\/2000\/01\/rdf-schema#label":[{"@value":"fluor_PAM","@type":"xsd:string"}],"http:\/\/ocean-data.org\/schema\/hasProcessingDescription":[{"@value":" BCO-DMO made the following changes:
E. C. Howard<\/strong>, S. Sun, C. R. Reisch, D. A. del Valle, R. P. Kiene, and M. A. Moran (2010). Changes in DMSP Demethylase Gene Assemblages in Response to an Induced Phytoplankton Bloom. Applied and Environmental Microbiology, vol. 77, p. 524. <\/strong>DOI: 10.1128\/AEM.01457-10<\/a>
Rinta-Kanto<\/strong>, H. Burgmann, S. M. Gifford, S. Sun, S. Sharma, R. P. Kiene, and M. A. Moran (2011). Analysis of Sulfur-Related Gene Expression by Roseobacter Communities Using a Taxon-Specific Functional Gene Microarray. Environmental Microbiology, vol. 13, p. 453. DOI: 10.1111\/j.1462-2920.2010.02350.x<\/a><\/p><\/div>","@type":"rdf:HTML"}],"http:\/\/ocean-data.org\/schema\/hasBriefDescription":[{"@value":"Pulse Amplitude Modulation (PAM) fluorometry readings from microcosm experiments.","@language":"en-US"}],"http:\/\/purl.org\/dc\/terms\/description":[{"@value":"
\n- Modified parameter names to conform with BCO-DMO naming conventions.
\n- Replaced blanks with 'nd' to indicate 'no data'.
\n- Separated date in day, month, and year columns.
\n- Added the site coordinates provided in the publications above.<\/p><\/div>","@type":"rdf:HTML"}],"http:\/\/purl.org\/dc\/terms\/identifier":[{"@value":"3873","@type":"xsd:int"}],"http:\/\/purl.org\/dc\/terms\/title":[{"@value":"fluor_PAM"}],"http:\/\/purl.org\/dc\/terms\/date":[{"@value":"2013-02-05T10:12:29-05:00","@type":"xsd:dateTime"}],"http:\/\/purl.org\/dc\/terms\/created":[{"@value":"2013-02-05T10:12:29-05:00","@type":"xsd:dateTime"}],"http:\/\/purl.org\/dc\/terms\/modified":[{"@value":"2023-07-07T16:10:26-04:00","@type":"xsd:dateTime"}],"http:\/\/rdfs.org\/ns\/void#inDataset":[{"@id":"http:\/\/www.bco-dmo.org\/"}],"http:\/\/ocean-data.org\/schema\/namedGraph":[{"@value":"urn:bcodmo:dataset:3873","@type":"xsd:token"}],"http:\/\/ocean-data.org\/schema\/osprey_page":[{"@id":"https:\/\/www.bco-dmo.org\/dataset\/3873"}],"http:\/\/ocean-data.org\/schema\/identifier":[{"@value":"_:Identifier3873"}],"http:\/\/ocean-data.org\/schema\/datasetTitle":[{"@value":"Pulse Amplitude Modulation (PAM) fluorometry readings from microcosm experiments from samples collected by R\/V E.O. 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Wilson in the Gulf of Mexico, Alabama (En-Gen DMSP Cycling project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). 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