http://lod.bco-dmo.org/id/dataset/3877
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2013-02-18
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Abundance results from microzooplankton experiments on the R/V Seward Johnson SJ0516 cruise between Ireland and Iceland during 2005, North Atlantic Spring Bloom (NASB 2005 project, Antarctic microzooplankton project)
2013-03-18
publication
2013-03-18
revision
BCO-DMO Linked Data URI
2013-03-18
creation
http://lod.bco-dmo.org/id/dataset/3877
Dr Julie Rose
National Oceanic and Atmospheric Administration
principalInvestigator
Christopher Gobler
Stony Brook University - SoMAS
principalInvestigator
David A. Hutchins
University of Southern California
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Rose, J., Hutchins, D. A., Gobler, C. (2013) Abundance results from microzooplankton experiments on the R/V Seward Johnson SJ0516 cruise between Ireland and Iceland during 2005, North Atlantic Spring Bloom (NASB 2005 project, Antarctic microzooplankton project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2013-02-18) Version Date 2013-03-18 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/3877 [access date]
Abundance results from temperature and pCO2 experiments on North Atlantic microzooplankton. Dataset Description: <p>The experiment was conducted onboard the RV Seward Johnson II, from June 20 to July 4, 2005, with water collected at 57 deg 58" N, 15 deg 32" W. Four treatments were used with 6 replicates each: (1) 12 deg C and 390 ppm CO<sub>2</sub> (LTLC), (2) 12 deg C and 690 ppm CO<sub>2</sub> (LTHC), (3) 16 deg C and 390 ppm CO<sub>2</sub> (HTLC), and (4) 16 deg C and 690 ppm CO<sub>2 </sub>(HTHC). Sea surface temperature at this location was 12°C at the time of water collection. Experiments were run using a seawater continuous culture system, termed an ‘Ecostat’ (Hutchins et al. 2003, Hare et al. 2005, 2007). Briefly, whole seawater was collected from 5 to 10 m depth using a trace-metal-clean, towed-intake Teflon pump system (Hutchins et al. 2003), prefiltered through 200 um Nitex mesh to remove mesozooplankton and incubated in twenty-four 2.7 l trace-metal-clean, clear polycarbonate bottles. Bottles were placed in racks in a temperature-controlled deck incubator with recirculating water and shaded to 30 percent of surface irradiance (I0) using a neutral-density shade screen. Temperatures in the 16 deg C incubator were gradually increased over a period of 24 h to avoid heat-shocking the plankton. Bottles were bubbled with either air or a commercially prepared air/CO2 mixture with 750 ppm CO2 using an inflow tube through the cap and an airstone to maximize gas transfer to the liquid phase. The gases used for bubbling were filtered through a 0.2 um HEPA filter to avoid contamination of experimental bottles by trace metals (Hare et al. 2005). The system was run in batch mode for 3 days prior to turning on the pumps, in order to stimulate phytoplankton growth and prevent wash-out of slower growing species. After this batch growth period, whole seawater in each incubation bottle was slowly diluted at a continuous rate using seawater collected at the initial site. This seawater medium was filtered through a 0.2 um inline capsule filter initially, then re-filtered through a second 0.2 um inline capsule filter immediately prior to use as a diluent. The medium was stored in trace-metal-clean, 50 l carboys in the dark. Initial in situ nutrient concentrations were low (0.32 umol nitrate l<sup>–1</sup>, 0.12 umol phosphate l<sup>–1</sup>, 0.7 umol silicate l<sup>–1</sup>), so the medium and the whole water in the incubation bottles were amended with 5 and 0.31 umol l<sup>–1</sup> (final concentration) of nitrate and phosphate. The dilution rate of 0.5 d<sup>–1</sup> was controlled in each incubation bottle using a peristaltic pump and calibrated daily to ensure constant flow rate. This flow rate constituted a 50 percent dilution of the experimental bottle volume daily. Incubation bottles were mixed by inverting the rack 120 degrees every 5 to 15 min using a compressed-air-driven system. Diluted seawater flowed out of the incubation bottles at a continuous rate and into 2.7 l polycarbonate bottles stored in the dark, which were used as outflow collection vessels. Seawater carbonate system measurements were performed as described in Feng et al. (2009).</p>
<p><strong>References:</strong><br />
Feng, Y., C.E. Hare, K. Leblanc, G.R. DiTullio, P.A. Lee, S.W. Wilhelm, J. Sun, J.M. Rose, N. Nemcek, I. Benner, and D.A. Hutchins. 2009. The effects of increased pCO2 and temperature on the North Atlantic Spring Bloom: I. The phytoplankton community and biogeochemical response. Marine Ecology Progress Series 388: 13-25.<br />
<br />
Hare, C.E., G.R. DiTullio, C.G. Trick, S.W. Wilhelm, K.W. Bruland, E.L. Rue, and D.A. Hutchins. 2005. Phytoplankton community structure changes following simulated upwelled iron inputs in the Peru upwelling region. Aquatic Microbial Ecology 38: 269-282.<br />
<br />
Hare, C.E., K. Leblanc, G.R. DiTullio, R.M. Kudela, Y. Zhang, P.A. Lee, S.F. Riseman, and D.A. Hutchins. 2007. Consequences of increased temperature and CO2 for phytoplankton community structure in the Bering Sea. Marine Ecology Progress Series 352: 9-16.<br />
<br />
Hutchins, D.A., F. Pustizzi, C.E. Hare, and G.R. DiTullio. 2003. A shipboard natural community continuous culture system for ecologically relevant low-level nutrient enrichment experiments. Limnology and Oceanography: Methods 1: 82-91.</p>
<p><strong>Related files and references:</strong><br />
Rose, J.M., Y. Feng, C.J. Gobler, R. Gutierrez, C.E. Hare, K. Leblanc, and D.A. Hutchins. 2009. The effects of increased pCO2 and temperature on the North Atlantic Spring Bloom. II. Microzooplankton abundance and grazing. Marine Ecology Progress Series 388: 27-40.<br />
<br />
Additional parameters measured during these experiments are described in: Feng, Y., C.E. Hare, K. Leblanc, G.R. DiTullio, P.A. Lee, S.W. Wilhelm, J. Sun, J.M. Rose, N. Nemcek, I. Benner, and D.A. Hutchins. 2009. The effects of increased pCO2 and temperature on the North Atlantic Spring Bloom: I. The phytoplankton community and biogeochemical response. Marine Ecology Progress Series 388: 13-25.<br />
<br />
Lee, P.A., J.R. Rudisill, A.R. Neeley, D.A. Hutchins, Y. Feng, C.E. Hare, K. Leblanc, J.M. Rose, S.W. Wilhelm, J.M. Rowe, and G.R. DiTullio. 2009. The effects of increased pCO2 and temperature on the North Atlantic Spring Bloom: III. Dimethylsulfoniopropionate. Marine Ecology Progress Series 388: 41-49.</p> Methods and Sampling: <p><strong>Microzooplankton abundance and community composition determined by light microscopy</strong><br />
Samples for enumeration of microzooplankton were removed from outflow collection bottles on Days 4, 9, and 14 of the experiment. Triplicate samples were counted on the initial day. Duplicate samples on Days 4 and 9 were mixed to obtain enough volume for accurate counts, yielding 3 replicates per treatment on these days. All 6 replicates were counted on the final day of the experiment. All samples were preserved with 10% acid Lugols solution and stored at room temperature in the dark until enumeration of microzooplankton in the laboratory (Throndsen 1978). Then, 100 ml of sample was settled for at least 18 h into Utermohl chambers before before counting using an inverted Zeiss Axiovert S100 microscope at 200× magnification (Utermohl 1958). Microzooplankton were identified to genus level when possible. The use of Lugol fixative obscured chl a fluorescence and rendered the distinction between phototrophic and heterotrophic dinoflagellates based on autofluorescence impossible. However, certain heterotrophic dinoflagellates such as Protoperidinium and Gyrodinium were identified based on morphology, and were thus included in the counts.</p>
<p><strong>References:</strong><br />
Throndsen, J. 1978. Preservation and storage. In Phytoplankton manual, ed. A. Sournia, 69-74. Paris: UNESCO.</p>
<p>Utermohl, H. 1958. Zur Vervollkommung der quantitativen phytoplankton-methodik. Mitteilungen der Internationalen Vereinigung fur Limnologie 9: 1-38.</p>
Funding provided by NSF Antarctic Sciences (NSF ANT) Award Number: PLR-0528715 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0528715
completed
Dr Julie Rose
National Oceanic and Atmospheric Administration
203-882-6544
Milford Laboratory 212 Roger Avenue
Milford
CT
06460
USA
pointOfContact
Christopher Gobler
Stony Brook University - SoMAS
School of Marine and Atmospheric Sciences Stony Brook University
Stony Brook
NY
11794-5000
USA
christopher.gobler@stonybrook.edu
pointOfContact
David A. Hutchins
University of Southern California
213-740-5616
Department of Biological Sciences 3616 Trousdale Parkway, AHF 20
Los Angeles
CA
90089
USA
dahutch@usc.edu
pointOfContact
asNeeded
Dataset Version: 2013-02-18
Unknown
treatment
day
bottle
total_microzoo
taxon
abundance
lat
lon
Inverted Microscope
theme
None, User defined
treatment
No BCO-DMO term
abundance
taxon
latitude
longitude
featureType
BCO-DMO Standard Parameters
Inverted Microscope
instrument
BCO-DMO Standard Instruments
SJ0516
service
Deployment Activity
North Atlantic Spring Bloom, largely between Ireland and Iceland
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
North Atlantic Spring Bloom 2005
https://osprey.bco-dmo.org/project/2070
North Atlantic Spring Bloom 2005
<p>Climate-related shifts in phytoplankton assemblages may have profound implications for oceanic feedbacks on the atmosphere, and for human use of marine resources. Particular algal groups are largely responsible for crucial processes like vertical carbon export, biogenic calcification and silicification, production of climatically active gases like dimethylsulfide (DMS), and for sustaining food webs that lead to economically valuable higher trophic levels. The North Atlantic Spring Bloom 2005 (NASB 2005) research program was designed to investigate potential climate change impacts on algal community structure and biogeochemistry during the North Atlantic Spring Bloom, a regime that is ideal for determining how changing ocean conditions may affect both calcareous and siliceous algae. </p>
<p>
The research was coordinated with CarboOcean, a major European Union funded activity led by investigators from the Alfred Wegener Institute. </p>
NASB 2005
largerWorkCitation
project
Rising climatic temperatures impact on antarctic microzooplankton growth and grazing
https://osprey.bco-dmo.org/project/2257
Rising climatic temperatures impact on antarctic microzooplankton growth and grazing
<p>The investigator will examine to what extent rising climatic temperatures impact antarctic microzooplankton growth and grazing, and to what extent such an impact would modulate top-down control of phytoplankton growth in cold waters. The experimental part of the proposed work would take place in the Ross Sea, a permanently cold ecosystem, and the location of annual large-scale blooms of both diatoms and Phaeocystis antarctica. Changing climate regimes may alter current microzooplankton grazing rates on these blooms either directly through temperature increases or indirectly through algal community shifts. Complementary laboratory experiments on cultures of Antarctic microzooplankton will be conducted to determine the individual and combined effects of temperature and carbon dioxide levels on growth and grazing.</p>
Antarctic microzooplankton
largerWorkCitation
project
eng; USA
oceans
North Atlantic Spring Bloom, largely between Ireland and Iceland
2013-03-18
From projects that focused on the following 2 locations: 1. North Atlantic 2. Ross Sea
0
BCO-DMO catalogue of parameters from Abundance results from microzooplankton experiments on the R/V Seward Johnson SJ0516 cruise between Ireland and Iceland during 2005, North Atlantic Spring Bloom (NASB 2005 project, Antarctic microzooplankton project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/33291.rdf
Name: treatment
Units: dimensionless
Description: <p>Experimental conditions varied in order to observe a variable's effect. Four treatments were used: (1) 12 deg C and 390 ppm CO2 (LTLC), (2) 12 deg C and 690 ppm CO2 (LTHC), (3) 16 deg C and 390 ppm CO2 (HTLC), and (4) 16 deg C and 690 ppm CO2 (HTHC).</p>
http://lod.bco-dmo.org/id/dataset-parameter/33292.rdf
Name: day
Units: dimensionless
Description: <p>Sampling day during experiment. The experiment was conducted from June 20 to July 4, 2005.</p>
http://lod.bco-dmo.org/id/dataset-parameter/33293.rdf
Name: bottle
Units: dimensionless
Description: <p>Experimental bottle number.</p>
http://lod.bco-dmo.org/id/dataset-parameter/33294.rdf
Name: total_microzoo
Units: cells per liter
Description: <p>Total microzooplankton cell count per liter of sample.</p>
http://lod.bco-dmo.org/id/dataset-parameter/33295.rdf
Name: taxon
Units: dimensionless
Description: <p>Genus name of identified microzooplankton (except 'nauplii', indicating life stage).</p>
http://lod.bco-dmo.org/id/dataset-parameter/33296.rdf
Name: abundance
Units: cells per liter
Description: <p>Total cell counts of genus per liter of sample.</p>
http://lod.bco-dmo.org/id/dataset-parameter/34362.rdf
Name: lat
Units: decimal degrees
Description: <p>Latitude component of geographic position where water was sampled.</p>
http://lod.bco-dmo.org/id/dataset-parameter/34363.rdf
Name: lon
Units: decimal degrees
Description: <p>Longitude component of geographic position where water was sampled.</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
34192
https://datadocs.bco-dmo.org/file/p99nDyoUMvnmD/microzoo_abund_Natl.csv
microzoo_abund_Natl.csv
Primary data file for dataset ID 3877
download
https://osprey.bco-dmo.org/dataset/3877/data/download
download
onLine
dataset
<p><strong>Microzooplankton abundance and community composition determined by light microscopy</strong><br />
Samples for enumeration of microzooplankton were removed from outflow collection bottles on Days 4, 9, and 14 of the experiment. Triplicate samples were counted on the initial day. Duplicate samples on Days 4 and 9 were mixed to obtain enough volume for accurate counts, yielding 3 replicates per treatment on these days. All 6 replicates were counted on the final day of the experiment. All samples were preserved with 10% acid Lugols solution and stored at room temperature in the dark until enumeration of microzooplankton in the laboratory (Throndsen 1978). Then, 100 ml of sample was settled for at least 18 h into Utermohl chambers before before counting using an inverted Zeiss Axiovert S100 microscope at 200× magnification (Utermohl 1958). Microzooplankton were identified to genus level when possible. The use of Lugol fixative obscured chl a fluorescence and rendered the distinction between phototrophic and heterotrophic dinoflagellates based on autofluorescence impossible. However, certain heterotrophic dinoflagellates such as Protoperidinium and Gyrodinium were identified based on morphology, and were thus included in the counts.</p>
<p><strong>References:</strong><br />
Throndsen, J. 1978. Preservation and storage. In Phytoplankton manual, ed. A. Sournia, 69-74. Paris: UNESCO.</p>
<p>Utermohl, H. 1958. Zur Vervollkommung der quantitativen phytoplankton-methodik. Mitteilungen der Internationalen Vereinigung fur Limnologie 9: 1-38.</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing Notes:</strong></p>
<p>- File was sorted by treatment<br />
- Added lat,lon values of original water sampling location to file<br />
- Added BCO-DMO header lines<br />
- Parameter names were edited to conform with BCO-DMO convention<br />
- File was transposed to serve data by taxon and abundance in columns<br />
- In data values representing mixed sampling bottles, plus signs (+) were edited to underscores(_)</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Inverted Microscope
Inverted Microscope
PI Supplied Instrument Name: Inverted Microscope PI Supplied Instrument Description:
/*-->*/
Counts were made using an inverted Zeiss Axiovert S100 microscope at 200× magnification (Utermohl 1958). Instrument Name: Inverted Microscope Instrument Short Name: Instrument Description: An inverted microscope is a microscope with its light source and condenser on the top, above the stage pointing down, while the objectives and turret are below the stage pointing up. It was invented in 1850 by J. Lawrence Smith, a faculty member of Tulane University (then named the Medical College of Louisiana).
Inverted microscopes are useful for observing living cells or organisms at the bottom of a large container (e.g. a tissue culture flask) under more natural conditions than on a glass slide, as is the case with a conventional microscope. Inverted microscopes are also used in micromanipulation applications where space above the specimen is required for manipulator mechanisms and the microtools they hold, and in metallurgical applications where polished samples can be placed on top of the stage and viewed from underneath using reflecting objectives.
The stage on an inverted microscope is usually fixed, and focus is adjusted by moving the objective lens along a vertical axis to bring it closer to or further from the specimen. The focus mechanism typically has a dual concentric knob for coarse and fine adjustment. Depending on the size of the microscope, four to six objective lenses of different magnifications may be fitted to a rotating turret known as a nosepiece. These microscopes may also be fitted with accessories for fitting still and video cameras, fluorescence illumination, confocal scanning and many other applications. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/
Cruise: SJ0516
SJ0516
Community Standard Description
International Council for the Exploration of the Sea
R/V Seward Johnson
vessel
SJ0516
David A. Hutchins
University of Southern California
Community Standard Description
International Council for the Exploration of the Sea
R/V Seward Johnson
vessel