http://lod.bco-dmo.org/id/dataset/3888
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2013-03-14
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Phytoplankton abundances from experiments on R/V Seward Johnson SJ0516 cruise between Ireland and Iceland during the 2005 North Atlantic Spring Bloom (NASB 2005 project, Antarctic microzooplankton project)
2013-03-14
publication
2013-03-14
revision
BCO-DMO Linked Data URI
2013-03-14
creation
http://lod.bco-dmo.org/id/dataset/3888
Dr Julie Rose
National Oceanic and Atmospheric Administration
principalInvestigator
Christopher Gobler
Stony Brook University - SoMAS
principalInvestigator
David A. Hutchins
University of Southern California
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Rose, J., Hutchins, D. A., Gobler, C. (2013) Phytoplankton abundances from experiments on R/V Seward Johnson SJ0516 cruise between Ireland and Iceland during the 2005 North Atlantic Spring Bloom (NASB 2005 project, Antarctic microzooplankton project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2013-03-14) Version Date 2013-03-14 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/3888 [access date]
phytoplankton abundances from temperature/pCO2 experiments on North Atlantic microzooplankton Dataset Description: <p><b>Experiment Description:</b> <br />
The experiment was conducted onboard the RV Seward Johnson II, from June 20 to July 4, 2005, with water collected at 57&deg; 58&rsquo; N, 15&deg; 32&rsquo;W. Four treatments were used with 6 replicates each: (1) 12&deg;C and 390 ppm CO<sub>2</sub> (LTLC), (2) 12&deg;C and 690 ppm CO<sub>2</sub> (LTHC), (3) 16&deg;C and 390 ppm CO<sub>2</sub> (HTLC), and (4) 16&deg;C and 690 ppm CO<sub>2 </sub>(HTHC). Sea surface temperature at this location was 12&deg;C at the time of water collection. Experiments were run using a seawater continuous culture system, termed an &lsquo;Ecostat&rsquo; (Hutchins et al. 2003, Hare et al. 2005, 2007). Briefly, whole seawater was collected from 5 to 10 m depth using a trace-metal-clean, towed-intake Teflon pump system (Hutchins et al. 2003), prefiltered through 200 &mu;m Nitex mesh to remove mesozooplankton and incubated in twenty-four 2.7 l trace-metal-clean, clear polycarbonate bottles. Bottles were placed in racks in a temperature-controlled deck incubator with recirculating water and shaded to 30 percent of surface irradiance (I0) using a neutral-density shade screen. Temperatures in the 16&deg;C incubator were gradually increased over a period of 24 h to avoid heat-shocking the plankton. Bottles were bubbled with either air or a commercially prepared air/CO2 mixture with 750 ppm CO2 using an inflow tube through the cap and an airstone to maximize gas transfer to the liquid phase. The gases used for bubbling were filtered through a 0.2 &mu;m HEPA filter to avoid contamination of experimental bottles by trace metals (Hare et al. 2005). The system was run in batch mode for 3 days prior to turning on the pumps, in order to stimulate phytoplankton growth and prevent wash-out of slower growing species. After this batch growth period, whole seawater in each incubation bottle was slowly diluted at a continuous rate using seawater collected at the initial site. This seawater medium was filtered through a 0.2 &mu;m inline capsule filter initially, then re-filtered through a second 0.2 &mu;m inline capsule filter immediately prior to use as a diluent. The medium was stored in trace-metal-clean, 50 l carboys in the dark. Initial in situ nutrient concentrations were low (0.32 umol nitrate l<sup>&ndash;1</sup>, 0.12 umol phosphate l<sup>&ndash;1</sup>, 0.7 umol silicate l<sup>&ndash;1</sup>), so the medium and the whole water in the incubation bottles were amended with 5 and 0.31 umol l<sup>&ndash;1</sup> (final concentration) of nitrate and phosphate. The dilution rate of 0.5 d<sup>&ndash;1</sup> was controlled in each incubation bottle using a peristaltic pump and calibrated daily to ensure constant flow rate. This flow rate constituted a 50 percent dilution of the experimental bottle volume daily. Incubation bottles were mixed by inverting the rack 120&deg; every 5 to 15 min using a compressed-air-driven system. Diluted seawater flowed out of the incubation bottles at a continuous rate and into 2.7 l polycarbonate bottles stored in the dark, which were used as outflow collection vessels.&nbsp; Seawater carbonate system measurements were performed as described in Feng et al. (2009).</p>
<p><b>References:</b><br />
Feng, Y., C.E. Hare, K. Leblanc, G.R. DiTullio, P.A. Lee, S.W. Wilhelm, J. Sun, J.M. Rose, N. Nemcek, I. Benner, and D.A. Hutchins. 2009. The effects of increased pCO2 and temperature on the North Atlantic Spring Bloom: I. The phytoplankton community and biogeochemical response. Marine Ecology Progress Series 388: 13-25.<br />
<br />
Hare, C.E., G.R. DiTullio, C.G. Trick, S.W. Wilhelm, K.W. Bruland, E.L. Rue, and D.A. Hutchins. 2005. Phytoplankton community structure changes following simulated upwelled iron inputs in the Peru upwelling region. Aquatic Microbial Ecology 38: 269-282.<br />
<br />
Hare, C.E., K. Leblanc, G.R. DiTullio, R.M. Kudela, Y. Zhang, P.A. Lee, S.F. Riseman, and D.A. Hutchins. 2007. Consequences of increased temperature and CO2 for phytoplankton community structure in the Bering Sea. Marine Ecology Progress Series 352: 9-16.<br />
<br />
Hutchins, D.A., F. Pustizzi, C.E. Hare, and G.R. DiTullio. 2003. A shipboard natural community continuous culture system for ecologically relevant low-level nutrient enrichment experiments. Limnology and Oceanography: Methods 1: 82-91.</p>
<p><b>Related files and references:</b><br />
Rose, J.M., Y. Feng, C.J. Gobler, R. Gutierrez, C.E. Hare, K. Leblanc, and D.A. Hutchins. 2009. The effects of increased pCO2 and temperature on the North Atlantic Spring Bloom. II. Microzooplankton abundance and grazing. Marine Ecology Progress Series 388: 27-40.<br />
<br />
Additional parameters measured during these experiments are described in: Feng, Y., C.E. Hare, K. Leblanc, G.R. DiTullio, P.A. Lee, S.W. Wilhelm, J. Sun, J.M. Rose, N. Nemcek, I. Benner, and D.A. Hutchins. 2009. The effects of increased pCO2 and temperature on the North Atlantic Spring Bloom: I. The phytoplankton community and biogeochemical response. Marine Ecology Progress Series 388: 13-25.<br />
<br />
Lee, P.A., J.R. Rudisill, A.R. Neeley, D.A. Hutchins, Y. Feng, C.E. Hare, K. Leblanc, J.M. Rose, S.W. Wilhelm, J.M. Rowe, and G.R. DiTullio. 2009. The effects of increased pCO2 and temperature on the North Atlantic Spring Bloom: III. Dimethylsulfoniopropionate. Marine Ecology Progress Series 388: 41-49.</p> Methods and Sampling: <p><b>Nanophytoplankton and picophytoplankton abundance measured by flow cytometry</b><br />
Nano- and picophytoplankton were enumerated using standard flow cytometric techniques (Olson et al. 1983, Yentsch et al. 1983). Samples were removed daily directly from experimental bottles, and 2 ml were preserved with 10% formalin buffered with seawater (1% final concentration), then frozen at &ndash;80&deg;C until analysis. Samples were analyzed in the laboratory on a Becton Dickinson FACSCalibur benchtop flow cytometer. Phytoplankton were identified in cytograms based on forward angle light scatter (size) and red fluorescence (chlorophyll). Phytoplankton were classified within cytograms as picoplankton (0.2 to 2 &mu;m) or nanoplankton (2 to 20 &mu;m) using 2 &mu;m green fluorescent beads (Invitrogen) added to each sample.</p>
<p><b>References:</b><br />
Olson, R.J., S.L. Frankel, S.W. Chisholm, and H.M. Shapiro. 1983. An inexpensive flow cytometer for the analysis of fluorescence signals in phytoplankton: chlorophyll and DNA distributions. Journal of Experimental Marine Biology and Ecology 68: 129-144.</p>
<p>Yentsch, C.M., P.K. Horan, K. Muirhead, Q. Dortch, E.M. Haugen, L. Legendre, L.S. Murphy, D. Phinney, S.A. Pomponi, R.W. Spinrad, A.M. Wood, C.S. Yentsch, and B.J. Zahurenec. 1983. Flow cytometry and sorting: a powerful technique with potential applications in aquatic sciences. Limnology and Oceanography 28: 1275-1280.</p>
Funding provided by NSF Antarctic Sciences (NSF ANT) Award Number: PLR-0528715 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0528715
onGoing
Dr Julie Rose
National Oceanic and Atmospheric Administration
203-882-6544
Milford Laboratory 212 Roger Avenue
Milford
CT
06460
USA
pointOfContact
Christopher Gobler
Stony Brook University - SoMAS
School of Marine and Atmospheric Sciences Stony Brook University
Stony Brook
NY
11794-5000
USA
christopher.gobler@stonybrook.edu
pointOfContact
David A. Hutchins
University of Southern California
213-740-5616
Department of Biological Sciences 3616 Trousdale Parkway, AHF 20
Los Angeles
CA
90089
USA
dahutch@usc.edu
pointOfContact
asNeeded
Dataset Version: 2013-03-14
Unknown
treatment
day
bottle
abund_nanophyto
abund_picophyto
sample
lat
lon
Flow Cytometer
theme
None, User defined
treatment
day of month
No BCO-DMO term
abundance
sample identification
latitude
longitude
featureType
BCO-DMO Standard Parameters
Flow Cytometer
instrument
BCO-DMO Standard Instruments
SJ0516
service
Deployment Activity
North Atlantic Spring Bloom, largely between Ireland and Iceland
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
North Atlantic Spring Bloom 2005
https://osprey.bco-dmo.org/project/2070
North Atlantic Spring Bloom 2005
<p>Climate-related shifts in phytoplankton assemblages may have profound implications for oceanic feedbacks on the atmosphere, and for human use of marine resources. Particular algal groups are largely responsible for crucial processes like vertical carbon export, biogenic calcification and silicification, production of climatically active gases like dimethylsulfide (DMS), and for sustaining food webs that lead to economically valuable higher trophic levels. The North Atlantic Spring Bloom 2005 (NASB 2005) research program was designed to investigate potential climate change impacts on algal community structure and biogeochemistry during the North Atlantic Spring Bloom, a regime that is ideal for determining how changing ocean conditions may affect both calcareous and siliceous algae. </p>
<p>
The research was coordinated with CarboOcean, a major European Union funded activity led by investigators from the Alfred Wegener Institute. </p>
NASB 2005
largerWorkCitation
project
Rising climatic temperatures impact on antarctic microzooplankton growth and grazing
https://osprey.bco-dmo.org/project/2257
Rising climatic temperatures impact on antarctic microzooplankton growth and grazing
<p>The investigator will examine to what extent rising climatic temperatures impact antarctic microzooplankton growth and grazing, and to what extent such an impact would modulate top-down control of phytoplankton growth in cold waters. The experimental part of the proposed work would take place in the Ross Sea, a permanently cold ecosystem, and the location of annual large-scale blooms of both diatoms and Phaeocystis antarctica. Changing climate regimes may alter current microzooplankton grazing rates on these blooms either directly through temperature increases or indirectly through algal community shifts. Complementary laboratory experiments on cultures of Antarctic microzooplankton will be conducted to determine the individual and combined effects of temperature and carbon dioxide levels on growth and grazing.</p>
Antarctic microzooplankton
largerWorkCitation
project
eng; USA
oceans
North Atlantic Spring Bloom, largely between Ireland and Iceland
2013-03-14
From projects that focused on the following 2 locations: 1. North Atlantic 2. Ross Sea
0
BCO-DMO catalogue of parameters from Phytoplankton abundances from experiments on R/V Seward Johnson SJ0516 cruise between Ireland and Iceland during the 2005 North Atlantic Spring Bloom (NASB 2005 project, Antarctic microzooplankton project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/33298.rdf
Name: treatment
Units: dimensionless
Description: <p>Experimental conditions varied in order to observe a variable's effect. Four treatments were used: (1) 12 deg C and 390 ppm CO2 (LTLC), (2) 12 deg C and 690 ppm CO2 (LTHC), (3) 16 deg C and 390 ppm CO2 (HTLC), and (4) 16 deg C and 690 ppm CO2 (HTHC).</p>
http://lod.bco-dmo.org/id/dataset-parameter/33299.rdf
Name: day
Units: dimensionless
Description: <p>Sampling day during experiment. The experiment was conducted from June 20 to July 4, 2005.</p>
http://lod.bco-dmo.org/id/dataset-parameter/33300.rdf
Name: bottle
Units: dimensionless
Description: <p>Experimental bottle number.</p>
http://lod.bco-dmo.org/id/dataset-parameter/33301.rdf
Name: abund_nanophyto
Units: cells per ml
Description: <p>Total cell counts of nanophytoplankton (2 to 20 um) per milliliter of sample.</p>
http://lod.bco-dmo.org/id/dataset-parameter/33302.rdf
Name: abund_picophyto
Units: cells per ml
Description: <p>Total cell counts of picophytoplankton (.2 to 2 um) per milliliter of sample.</p>
http://lod.bco-dmo.org/id/dataset-parameter/33303.rdf
Name: sample
Units: dimensionless
Description: <p>Unique alphanumeric characters identifying each sample.</p>
http://lod.bco-dmo.org/id/dataset-parameter/34594.rdf
Name: lat
Units: decimal degrees
Description: <p>Latitude component of geographic position where water was sampled.</p>
http://lod.bco-dmo.org/id/dataset-parameter/34595.rdf
Name: lon
Units: decimal degrees
Description: <p>Longitude component of geographic position where water was sampled.</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
7111
https://datadocs.bco-dmo.org/file/A88rL37SNzp19q/phyto_abund.csv
phyto_abund.csv
Primary data file for dataset ID 3888
download
https://osprey.bco-dmo.org/dataset/3888/data/download
download
onLine
dataset
<p><b>Nanophytoplankton and picophytoplankton abundance measured by flow cytometry</b><br />
Nano- and picophytoplankton were enumerated using standard flow cytometric techniques (Olson et al. 1983, Yentsch et al. 1983). Samples were removed daily directly from experimental bottles, and 2 ml were preserved with 10% formalin buffered with seawater (1% final concentration), then frozen at &ndash;80&deg;C until analysis. Samples were analyzed in the laboratory on a Becton Dickinson FACSCalibur benchtop flow cytometer. Phytoplankton were identified in cytograms based on forward angle light scatter (size) and red fluorescence (chlorophyll). Phytoplankton were classified within cytograms as picoplankton (0.2 to 2 &mu;m) or nanoplankton (2 to 20 &mu;m) using 2 &mu;m green fluorescent beads (Invitrogen) added to each sample.</p>
<p><b>References:</b><br />
Olson, R.J., S.L. Frankel, S.W. Chisholm, and H.M. Shapiro. 1983. An inexpensive flow cytometer for the analysis of fluorescence signals in phytoplankton: chlorophyll and DNA distributions. Journal of Experimental Marine Biology and Ecology 68: 129-144.</p>
<p>Yentsch, C.M., P.K. Horan, K. Muirhead, Q. Dortch, E.M. Haugen, L. Legendre, L.S. Murphy, D. Phinney, S.A. Pomponi, R.W. Spinrad, A.M. Wood, C.S. Yentsch, and B.J. Zahurenec. 1983. Flow cytometry and sorting: a powerful technique with potential applications in aquatic sciences. Limnology and Oceanography 28: 1275-1280.</p>
Specified by the Principal Investigator(s)
<p><b>BCO-DMO Processing Notes:</b></p>
<p>- File was sorted by treatment<br />
- Added lat, lon values of original water sampling location to file<br />
- Added BCO-DMO header lines<br />
- Parameter names were edited to conform with BCO-DMO convention<br />
- Edited abundance precision to 3 decimals as per the PI</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Flow Cytometer
Flow Cytometer
PI Supplied Instrument Name: Flow Cytometer PI Supplied Instrument Description:Samples were analyzed in the laboratory on a Becton Dickinson FACSCalibur benchtop flow cytometer. Instrument Name: Flow Cytometer Instrument Short Name:Flow Cytometer Instrument Description: Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells.
(from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm) Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB37/
Cruise: SJ0516
SJ0516
Community Standard Description
International Council for the Exploration of the Sea
R/V Seward Johnson
vessel
SJ0516
David A. Hutchins
University of Southern California
Community Standard Description
International Council for the Exploration of the Sea
R/V Seward Johnson
vessel