http://lod.bco-dmo.org/id/dataset/3901
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2013-03-25
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Phytoplankton (Prochlorococcus, Synechococcus, and PEuks) determined by flow cytometry of samples collected on R/V Thomas G. Thompson and R/V Kilo Moana cruises TN277, KM1301, KM1312 in the Eastern North Pacific Ocean from 2012-2013 (POWOW project)
2014-09-10
publication
2014-09-10
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2021-12-28
publication
https://doi.org/10.26008/1912/bco-dmo.3901.4
Zackary I. Johnson
Duke University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Johnson, Z. I. (2021) Phytoplankton (Prochlorococcus, Synechococcus, and PEuks) determined by flow cytometry of samples collected on R/V Thomas G. Thompson and R/V Kilo Moana cruises TN277, KM1301, KM1312 in the Eastern North Pacific Ocean from 2012-2013 (POWOW project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 4) Version Date 2014-09-10 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.3901.4 [access date]
Phytoplankton (Prochlorococcus, Synechococcus, and PEuks) by flow cytometry. Dataset Description: <p>Phytoplankton concentrations from POWOW1 (TN277), POWOW2 (KM1301), and POWOW3 (KM1312) cruises including Prochlorococcus, Synechococcus and pico-photosynthetic eukaryotes (PEuks) determined by flow cytometry.</p> Methods and Sampling: <p>Phytoplankton were enumerated using a FACSCalibur flow cytometer (Becton Dickinson) and populations characterized as previously described (Johnson et al., 2010). Briefly, cells were excited with a 488 nm laser (15 mW Ar) and inelastic forward (&lt;15°) scatter, inelastic side (90°) scatter (SSC), green (530 ± 30 nm) fluorescence, orange fluorescence (585 ± 42 nm), and red fluorescence (&gt; 670 nm) emissions were measured. Populations were deemed ‘phytoplankton’ if they contained red-fluorescence, i.e. had a chlorophyll signal and were subsequently assigned to specific taxa (i.e. Prochlorococcus, Synechococcus or Pico-photosynthetic eukaryotes) based on previous classification schemes (Johnson et al., 2010).</p>
<p>References:<br />
Johnson, Z.I., Shyam, R., Ritchie, A.E., Lin, Y., Mioni, C., Lance, V.P. et al. (2010) The effects of iron- and light-limitation on phytoplankton communities of deep chlorophyll maxima of the Western Pacific Ocean. Journal of Marine Research 68: 1-26. doi: <a href="http://dx.doi.org/10.1357/002224010793721433" target="_blank">10.1357/002224010793721433</a></p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1031064 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1031064
completed
Zackary I. Johnson
Duke University
1-252-504-7543
Marine Sciences and Conservation 135 Duke Marine Lab Rd.
Beaufort
NC
28516
USA
zij@duke.edu
pointOfContact
asNeeded
Dataset Version: 4
Unknown
cruise_name
cast
lat
lon
month_utc
day_utc
year
depth
bot
prochlorococcus
synechococcus
pico_euks
depth_w
time_utc
Flow Cytometer
theme
None, User defined
cruise name
cast
latitude
longitude
month_utc
day_utc
year
depth
bottle number
prochlorococcus abundance
synechococcus abundance
pico_euks
depth_w
time_utc
featureType
BCO-DMO Standard Parameters
Flow Cytometer
instrument
BCO-DMO Standard Instruments
TN277
KM1301
KM1312
service
Deployment Activity
Eastern North Pacific Ocean
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Seasonal and decadal changes in temperature drive Prochlorococcus ecotype distribution patterns
http://oceanography.ml.duke.edu/johnson/research/powow/
Seasonal and decadal changes in temperature drive Prochlorococcus ecotype distribution patterns
<p>Project also known as '<em>Prochlorococcus</em> Of Warming Ocean Waters' (POWOW).</p>
<p>The two numerically-dominant ecotypes of the marine cyanobacterium <em>Prochlorococcus</em> partition the surface ocean niche latitudinally, with ecotype eMIT9312 dominant in the 30 degree N to 30 degree S region and eMED4 dominant at higher latitudes. These ecotypes may account for 25-50% of primary production in open ocean ecosystems, but this percentage is dependent on which ecotype dominates. The relative abundance of the two ecotypes follows a log-linear relationship with temperature, with the transition from eMIT9312 to eMED4 occurring at approx. 18 degrees C. From these descriptive data, it has been hypothesized that temperature is the primary driver of relative abundance. Their contribution to net primary production, however, appears to be independent of temperature, suggesting temperature regulates ecotype dominance through photosynthesis-independent mechanisms.</p>
<p>To test these hypotheses, the PIs are undertaking a series of field and lab studies to investigate the effect of temperature change on the distribution of these ecotypes. Two cruises in the North Pacific will trace the transitions from eMIT9312- to eMED4-dominated regions, with one cruise during the winter and the other during summer. They have hypothesized that the ratio of ecotype abundance will move latitudinally with the seasonal shift in temperature gradient: migration of the 18 degrees C isotherm northward in the summer will be matched by a similar migration of the 1:1 ecotype transition point. Multiple crossings of the 18 degrees C isotherm are proposed, and the summer cruise will also follow the isotherm to the Western US coast to gain insight on physical and geochemical influences. Environmental variables such as nutrient concentrations, light/mixing depths, and virus /grazing based mortality, which may impinge on the relationship between temperature and ecotype ratio, will be assessed through a series of multivariate analyses of the collected suite of physical, chemical and biological data. Seasonal comparisons will be complemented with on-deck incubations and lab competition assays (using existing and new isolates) that will establish, for the first time, how fitness coefficients of these ecotypes relate to temperature. As latitudinal shifts in temperature gradient and migration of ecotypes during seasonal warming likely share common features with high latitude warming as a consequence of climate change, the investigator's analyses will contribute important biological parameters (e.g., abundances, production rates, temperature change coefficients) for modeling biological and biogeochemical responses to climate change. This research will be integrated with that of committed collaborators, generating data sufficient for ecosystem-scale characterizations of the contributions of temperature (relative to other forcing factors) in constraining the range and seasonal migration of these numerically dominant marine phototrophs.</p>
<p><strong>Publications produced as result of this research:</strong><br />
Rowe, J.M., DeBruyn, J.M., Poorvin, L., LeCleir, G.R., Johnson, Z.I., Zinser, E.R., and Wilhelm, S.W. 2012. Viral and bacterial abundance and production in the Western Pacific Ocean and the relation to other oceanic realms. FEMS Microbiology Ecology, 72, p. 359. DOI: <a href="http://dx.doi.org/10.1111/j.1574-6941.2011.01223.x" target="_blank">10.1111/j.1574-6941.2011.01223.x</a></p>
<p>Morris, J.J., Lenski, R.E. and E.R. Zinser. 2012. The Black Queen Hypothesis: Evolution of Dependencies through Adaptive Gene Loss. mBio, 3, p. e00036-12. DOI: <a href="http://dx.doi.org/10.1128/mBio.00036-12" target="_blank">10.1128/mBio.00036-12</a></p>
<p>Morris, J.J., Johnson, Z.I., Szul, M.J., Keller, M., and Zinser, E.R. 2011. Dependence of the cyanobacterium <em>Prochlorococcus</em> on hydrogen peroxide scavenging microbes for growth at the ocean's surface. PLoS One, 6(2), p. 16805. DOI:<a href="http://dx.doi.org/10.1371/journal.pone.0016805" target="_blank">10.1371/journal.pone.0016805</a></p>
<p>Ringuet, S., Sassano, L., and Johnson, Z.I. 2011. A suite of microplate reader-based colorimetric methods to quantify ammonium, nitrate, orthophosphate and silicate concentrations for aquatic nutrient monitoring. Journal of Environmental Monitoring. DOI:<a href="http://dx.doi.org/10.1039/C0EM00290A" target="_blank">10.1039/C0EM00290A</a></p>
<p>Ritchie, A.E. and Johnson, Z.I. 2012. Abundance and genetic diversity of aerobic anoxygenic phototrophic bacteria of coastal regions of the Pacific Ocean. Applied and Environmental Microbiology, 78, p. 2858. DOI: <a href="http://dx.doi.org/10.1128/AEM.06268-11" target="_blank">10.1128/AEM.06268-11</a></p>
POWOW
largerWorkCitation
project
eng; USA
oceans
Eastern North Pacific Ocean
-160.6166
-118.316
21.3428
46.6888
2012-03-01
2013-07-27
Eastern North Pacific Ocean
0
BCO-DMO catalogue of parameters from Phytoplankton (Prochlorococcus, Synechococcus, and PEuks) determined by flow cytometry of samples collected on R/V Thomas G. Thompson and R/V Kilo Moana cruises TN277, KM1301, KM1312 in the Eastern North Pacific Ocean from 2012-2013 (POWOW project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/33451.rdf
Name: cruise_name
Units: text
Description: <p>Cruise identifier (POWOW1 = TN277 = R/V Thomas G. Thompson cruise 277; POWOW2 = KM1301 = R/V Kilo Moana cruise 1301).</p>
http://lod.bco-dmo.org/id/dataset-parameter/33452.rdf
Name: cast
Units: unitless
Description: <p>CTD cast number.</p>
http://lod.bco-dmo.org/id/dataset-parameter/33453.rdf
Name: lat
Units: decimal degrees
Description: <p>Latitude in decimal degrees. Positive = North.</p>
http://lod.bco-dmo.org/id/dataset-parameter/33454.rdf
Name: lon
Units: decimal degrees
Description: <p>Longitude in decimal degrees. Positive = East.</p>
http://lod.bco-dmo.org/id/dataset-parameter/33455.rdf
Name: month_utc
Units: mm (01 to 12)
Description: <p>2-digit month of year, UTC.</p>
http://lod.bco-dmo.org/id/dataset-parameter/33456.rdf
Name: day_utc
Units: dd (01 to 31)
Description: <p>2-digit day of month, UTC.</p>
http://lod.bco-dmo.org/id/dataset-parameter/33457.rdf
Name: year
Units: YYYY
Description: <p>4-digit year.</p>
http://lod.bco-dmo.org/id/dataset-parameter/33458.rdf
Name: depth
Units: meters
Description: <p>Sample depth.</p>
http://lod.bco-dmo.org/id/dataset-parameter/33459.rdf
Name: bot
Units: unitless
Description: <p>Rosette position of the bottle.</p>
http://lod.bco-dmo.org/id/dataset-parameter/33460.rdf
Name: prochlorococcus
Units: cells per milliliter
Description: <p>Concentration of Prochlorococcus (cells/mL).</p>
http://lod.bco-dmo.org/id/dataset-parameter/33461.rdf
Name: synechococcus
Units: cells per milliliter
Description: <p>Concentration of Synechococcus (cells/mL).</p>
http://lod.bco-dmo.org/id/dataset-parameter/33462.rdf
Name: pico_euks
Units: cells per milliliter
Description: <p>Concentration of pico-photosynthetic eukaryotes, or PEuks (cells/mL).</p>
http://lod.bco-dmo.org/id/dataset-parameter/514863.rdf
Name: depth_w
Units: meters
Description: <p>Depth of the water (bottom depth).</p>
http://lod.bco-dmo.org/id/dataset-parameter/514866.rdf
Name: time_utc
Units: HHMM
Description: <p>Time, in hours and minutes, UTC; 24-hour clock.</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
63607
https://darchive.mblwhoilibrary.org/bitstream/1912/27876/1/dataset-3901_phytoplankton__v4.tsv
download
https://doi.org/10.26008/1912/bco-dmo.3901.4
download
onLine
dataset
<p>Phytoplankton were enumerated using a FACSCalibur flow cytometer (Becton Dickinson) and populations characterized as previously described (Johnson et al., 2010). Briefly, cells were excited with a 488 nm laser (15 mW Ar) and inelastic forward (&lt;15°) scatter, inelastic side (90°) scatter (SSC), green (530 ± 30 nm) fluorescence, orange fluorescence (585 ± 42 nm), and red fluorescence (&gt; 670 nm) emissions were measured. Populations were deemed ‘phytoplankton’ if they contained red-fluorescence, i.e. had a chlorophyll signal and were subsequently assigned to specific taxa (i.e. Prochlorococcus, Synechococcus or Pico-photosynthetic eukaryotes) based on previous classification schemes (Johnson et al., 2010).</p>
<p>References:<br />
Johnson, Z.I., Shyam, R., Ritchie, A.E., Lin, Y., Mioni, C., Lance, V.P. et al. (2010) The effects of iron- and light-limitation on phytoplankton communities of deep chlorophyll maxima of the Western Pacific Ocean. Journal of Marine Research 68: 1-26. doi: <a href="http://dx.doi.org/10.1357/002224010793721433" target="_blank">10.1357/002224010793721433</a></p>
Specified by the Principal Investigator(s)
<p>Cells counts were normalized to volume sampled to determined cells per mL.</p>
<p><strong>BCO-DMO edits made:</strong><br />
- Parameter names have been changed to conform to BCO-DMO conventions.<br />
- month_utc, day_utc, and year were added, based on the original date field.<br />
- Original version of POWOW1 data (dated 25 March 2013) replaced with updated version 28 May 2013; POWOW2 added 14 May 2013.<br />
- Replaced 'NaN' with 'nd' to indicate 'no data'.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Flow Cytometer
Flow Cytometer
PI Supplied Instrument Name: Flow Cytometer PI Supplied Instrument Description:A FACSCalibur flow cytometer (Becton Dickinson) was used to enumerate phytoplankton. Instrument Name: Flow Cytometer Instrument Short Name:Flow Cytometer Instrument Description: Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells.
(from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm) Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB37/
Cruise: TN277
TN277
R/V Thomas G. Thompson
Community Standard Description
International Council for the Exploration of the Sea
R/V Thomas G. Thompson
vessel
TN277
Zackary I. Johnson
Duke University
http://dmoserv3.whoi.edu/data_docs/POWOW/POWOW1-cruise_report.pdf
Report describing TN277
Cruise: KM1301
KM1301
R/V Kilo Moana
Community Standard Description
International Council for the Exploration of the Sea
R/V Kilo Moana
vessel
KM1301
Zackary I. Johnson
Duke University
http://dmoserv3.whoi.edu/data_docs/POWOW/POWOW2-cruise_report.pdf
Report describing KM1301
Cruise: KM1312
KM1312
R/V Kilo Moana
Community Standard Description
International Council for the Exploration of the Sea
R/V Kilo Moana
vessel
KM1312
Zackary I. Johnson
Duke University
http://dmoserv3.whoi.edu/data_docs/POWOW/POWOW3-cruise_report.pdf
Report describing KM1312
R/V Thomas G. Thompson
Community Standard Description
International Council for the Exploration of the Sea
R/V Thomas G. Thompson
vessel
R/V Kilo Moana
Community Standard Description
International Council for the Exploration of the Sea
R/V Kilo Moana
vessel