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Abundance of bacteria, heterotrophic nanoflagellates, diatoms, Phaeocystis antarctica and other phytoplankton was determined for samples preserved with 1% seawater-buffered, 0.2 um-filtered formalin (final concentration), filtered under low vacuum and examined under epifluorescence microscopy.\u00a0 For bacteria, 5-10 ml sample were filtered onto a 0.2 um black polycarbonate filter, stained with Vectashield Mounting Medium with DAPI (Vector Laboratories) and stored on glass slides at -20 deg C until analysis.\u00a0 For phytoplankton, 10-30 ml samples were filtered onto 0.8 um black polycarbonate filters and stored on glass slides at -20 deg C until analysis.\u00a0 For heterotrophic nanoflagellates, 10-50 ml samples were filtered onto 0.8 um black polycarbonate filters, stained with Vectashield Mounting Medium with DAPI (Vector Laboratories) and stored on glass slides at -20 deg C until analysis.<\/p>\n
Throndsen, J. 1978. Preservation and storage. In Phytoplankton manual, ed. A. Sournia, 69-74. Paris: UNESCO.<\/p>\n
Uterm\u00f6hl, H. 1958. Zur Vervollkommung der quantitativen phytoplankton-methodik. Mitteilungen der Internationalen Vereinigung f\u00fcr Limnologie 9: 1-38.<\/p><\/div>","@type":"rdf:HTML"}],"http:\/\/ocean-data.org\/schema\/hasBriefDescription":[{"@value":"Plankton abundances from Antarctic phytoplankton experiments","@language":"en-US"}],"http:\/\/purl.org\/dc\/terms\/description":[{"@value":"
Abundances of bacteria, heterotrophic nanoflagellates, diatoms, and other phytoplankton from temperature and light experiments on Antarctic phytoplankton and microzooplankton.<\/p>\n
Experimental Design:<\/p>\n
Experiments were conducted during the CORSACS (Controls On Ross Sea Algal Community Structure) expedition in November 2006 to the Ross Sea, Antarctica, onboard the RVIB Nathaniel B. Palmer (cruise NBP-0608). Water was collected at 76 50' S, 173 47' E using a trace metal clean towed-intake surface water Teflon diaphragm pumping system (Bruland et al., 2005). Sea surface temperature at this location was -1.5 deg C at the time of water collection. Water was prescreened through acid-washed 200 um Nitex mesh to eliminate large zooplankton and collected into a 50-L mixing carboy. Collected water was gently mixed and dispensed into 24 4.5-L acid washed trace metal clean clear polycarbonate bottles for incubation. Four treatments were used with six replicates per treatment. Bottles were incubated in two temperature controlled deck-board incubators housed in deck vans under halogen lights (Feng et al., 2009; Hare et al., 2007). Irradiance was 2000 uE m2 s-1 without screening. Incubators were screened with neutral density filter and measured irradiances in the four treatments were:<\/p>\n
Low light, low temperature (LLLT): 61 uE m2 s-1
\nLow light, high temperature treatment (LLHT): 45 uE m2 s-1
\nHigh light, low temperature (HLLT): 321 uE m2 s-1
\nHigh light, high temperature (HLHT): 320 uE m2 s-1<\/p>\n
\nOne incubator was maintained at 0 deg C, while the temperature in the other was gradually increased to 4 deg C over the course of 24 h. Bottles were incubated for eight days.\u00a0 All sampling occurred under a laminar flow hood using trace metal clean techniques.<\/p>\n
Bruland, K.W., E.L. Rue, G.J. Smith, and G.R. DiTullio. 2005. Iron, macronutrients and diatom blooms in the Peru upwelling regime: brown and blue waters of Peru. Marine Chemistry 93: 81-103.<\/p>\n
Feng, Y., C.E. Hare, K. Leblanc, G.R. DiTullio, P.A. Lee, S.W. Wilhelm, J. Sun, J.M. Rose, N. Nemcek, I. Benner, and D.A. Hutchins. 2009. The effects of increased pCO2 and temperature on the North Atlantic Spring Bloom: I. The phytoplankton community and biogeochemical response. Marine Ecology Progress Series 388: 13-25.<\/p>\n
Hare, C.E., K. Leblanc, G.R. DiTullio, R.M. Kudela, Y. Zhang, P.A. Lee, S.F. Riseman, and D.A. Hutchins. 2007. Consequences of increased temperature and CO2 for phytoplankton community structure in the Bering Sea. Marine Ecology Progress Series 352: 9-16.
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BCO-DMO Processing Notes:<\/strong><\/p>\n - File was sorted by treatment
\n- Added lat,lon values of original water sampling location to file
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