<div><p><strong>Maximum quantum yield efficiency for PSII (F<sub>v</sub>/F<sub>m</sub>)</strong><br />
Photochemical efficiency of PSII was measured using a MBARI 4th generation bench-top fast repetition rate fluorometer (FRRF) (Kolber et al., 1994). Samples were collected each day from experimental bottles, immediately placed on ice and kept in low light conditions (5 - 10 mol photons m−2 s-1) for 30 - 40 min prior to analysis. The light and cuvette chamber were constantly flushed with dry nitrogen gas to avoid condensation on the exterior of the cuvette due to the temperature difference between the cold seawater and the laboratory air. Minimal (F<sub>0</sub>) and maximal (F<sub>m</sub>) fluorescence and the effective absorption cross section (o'PSII) were calculated from each single turnover (ST) saturation curve. The maximum quantum yield efficiency for PSII (F<sub>v</sub>/F<sub>m</sub>) was calculated (Genty et al., 1989) by normalizing F<sub>m</sub> by the difference between the fluorescence at saturation (F<sub>m</sub>) and the minimum fluorescence (F<sub>0</sub>):</p>
<p>Genty, B., J.M. Briantais, and N.R. Baker. 1989. The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochimica et Biophysica Acta 990: 87-92.</p>
<p>Kolber, Z.S., R.T. Barber, K.H. Coale, S.E. Fitzwater, R.M. Greene, K.S. Johnson, S. Lindley, and G.P. Falkowski. 1994. Iron limitation of the phytoplankton photosynthesis in the equatorial Pacific Ocean. Nature 371: 145-149.</p></div>
Photosystem efficiency data from temperature and light experiments involving Antarctic phytoplankton.
<div><p><strong>Experimental Design:</strong></p>
<p>Experiments were conducted during the CORSACS (Controls On Ross Sea Algal Community Structure) expedition in November 2006 to the Ross Sea, Antarctica, onboard the RVIB Nathaniel B. Palmer (cruise NBP-0608). Water was collected at 76 50' S, 173 47' E using a trace metal clean towed-intake surface water Teflon diaphragm pumping system (Bruland et al., 2005). Sea surface temperature at this location was -1.5 deg C at the time of water collection. Water was prescreened through acid-washed 200 um Nitex mesh to eliminate large zooplankton and collected into a 50-L mixing carboy. Collected water was gently mixed and dispensed into 24 4.5-L acid washed trace metal clean clear polycarbonate bottles for incubation. Four treatments were used with six replicates per treatment. Bottles were incubated in two temperature controlled deck-board incubators housed in deck vans under halogen lights (Feng et al., 2009; Hare et al., 2007). Irradiance was 2000 uE m2 s-1 without screening. Incubators were screened with neutral density filter and measured irradiances in the four treatments were:</p>
<p>Low light, low temperature (LLLT): 61 uE m2 s-1<br />
Low light, high temperature treatment (LLHT): 45 uE m2 s-1<br />
High light, low temperature (HLLT): 321 uE m2 s-1<br />
High light, high temperature (HLHT): 320 uE m2 s-1</p>
<p>
One incubator was maintained at 0 deg C, while the temperature in the other was gradually increased to 4 deg C over the course of 24 h. Bottles were incubated for eight days. All sampling occurred under a laminar flow hood using trace metal clean techniques.</p>
<p>Bruland, K.W., E.L. Rue, G.J. Smith, and G.R. DiTullio. 2005. Iron, macronutrients and diatom blooms in the Peru upwelling regime: brown and blue waters of Peru. Marine Chemistry 93: 81-103.</p>
<p>Feng, Y., C.E. Hare, K. Leblanc, G.R. DiTullio, P.A. Lee, S.W. Wilhelm, J. Sun, J.M. Rose, N. Nemcek, I. Benner, and D.A. Hutchins. 2009. The effects of increased pCO2 and temperature on the North Atlantic Spring Bloom: I. The phytoplankton community and biogeochemical response. Marine Ecology Progress Series 388: 13-25.</p>
<p>Hare, C.E., K. Leblanc, G.R. DiTullio, R.M. Kudela, Y. Zhang, P.A. Lee, S.F. Riseman, and D.A. Hutchins. 2007. Consequences of increased temperature and CO2 for phytoplankton community structure in the Bering Sea. Marine Ecology Progress Series 352: 9-16.<br />
</p></div>
photosystem_efficiency_Ant2
<div><p><strong>BCO-DMO Processing Notes:</strong></p>
<p>- File was sorted by treatment<br />
- Added lat,lon values of original water sampling location to file<br />
- Added BCO-DMO header lines<br />
- Parameter names were edited to conform with BCO-DMO convention</p></div>
3934
photosystem_efficiency_Ant2
2013-05-01T11:14:57-04:00
2013-05-01T11:14:57-04:00
2023-07-07T16:10:26-04:00
urn:bcodmo:dataset:3934
Photosystem efficiency from phytoplankton experiments on the RVIB Nathaniel B. Palmer NBP0608 cruise in the Ross Sea Southern Ocean during 2006 (CORSACS project, Antarctic microzooplankton project)
false
Rose, J., Hutchins, D. A. (2013) Photosystem efficiency from phytoplankton experiments on the RVIB Nathaniel B. Palmer NBP0608 cruise in the Ross Sea Southern Ocean during 2006 (CORSACS project, Antarctic microzooplankton project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2013-04-29) Version Date 2013-04-29 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/3934 [access date]
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2013-04-29
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2013-04-29
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