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            <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/dataset/3945.rdf" xlink:actuate="onRequest">BioChemical results from the CO2 Aggregation Experiment from UCSB Marine Science Institute Passow Lab from 2009 to 2010 (OA - Ocean Acidification and Aggregation project)</gmx:Anchor>
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&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Reference:&amp;lt;/strong&amp;gt; Passow, U., Rocha, C.L.D.L., Fairfield, C., Schmidt, K., 2014. Aggregation as a function of pCO2 and mineral particles. Limnology and Oceanography 59 (2), 532-547.&amp;lt;/p&amp;gt; Methods and Sampling: &amp;lt;p&amp;gt;See: &amp;lt;a href=&amp;quot;http://bcodata.whoi.edu/Ocean_Acidification_and_Aggregation/Series2_CO2_Aggregation_Experiment_Method.pdf&amp;quot; target=&amp;quot;_blank&amp;quot;&amp;gt;Series 2: CO2 Aggregation Experiment - Methods&amp;lt;/a&amp;gt;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;BCO-DMO Note:&amp;lt;/strong&amp;gt;&amp;lt;br /&amp;gt;
Instruments listed apply to all phases of the Series 2 data.&amp;lt;br /&amp;gt;
Not just the experiment(s) represented in the individual dataset.&amp;lt;/p&amp;gt;</gco:CharacterString>
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In recognition of the need for basic research concerning the nature, extent and impact of ocean acidification on oceanic environments in the past, present and future, the goal of the SEES: OA program is to understand (a) the chemistry and physical chemistry of ocean acidification; (b) how ocean acidification interacts with processes at the organismal level; and (c) how the earth system history informs our understanding of the effects of ocean acidification on the present day and future ocean.
Solicitations issued under this program:
NSF 10-530, FY 2010-FY2011
NSF 12-500, FY 2012
NSF 12-600, FY 2013
NSF 13-586, FY 2014
NSF 13-586 was the final solicitation that will be released for this program.
PI Meetings:
1st U.S. Ocean Acidification PI Meeting(March 22-24, 2011, Woods Hole, MA)
2nd U.S. Ocean Acidification PI Meeting(Sept. 18-20, 2013, Washington, DC)
3rd U.S. Ocean Acidification PI Meeting (June 9-11, 2015, Woods Hole, MA – Tentative)
NSF media releases for the Ocean Acidification Program:
Press Release 10-186 NSF Awards Grants to Study Effects of Ocean Acidification
Discovery Blue Mussels &quot;Hang On&quot; Along Rocky Shores: For How Long?
Discovery nsf.gov - National Science Foundation (NSF) Discoveries - Trouble in Paradise: Ocean Acidification This Way Comes - US National Science Foundation (NSF)
Press Release 12-179 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: Finding New Answers Through National Science Foundation Research Grants - US National Science Foundation (NSF)
Press Release 13-102 World Oceans Month Brings Mixed News for Oysters
Press Release 13-108 nsf.gov - National Science Foundation (NSF) News - Natural Underwater Springs Show How Coral Reefs Respond to Ocean Acidification - US National Science Foundation (NSF)
Press Release 13-148 Ocean acidification: Making new discoveries through National Science Foundation research grants
Press Release 13-148 - Video nsf.gov - News - Video - NSF Ocean Sciences Division Director David Conover answers questions about ocean acidification. - US National Science Foundation (NSF)
Press Release 14-010 nsf.gov - National Science Foundation (NSF) News - Palau's coral reefs surprisingly resistant to ocean acidification - US National Science Foundation (NSF)
Press Release 14-116 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: NSF awards $11.4 million in new grants to study effects on marine ecosystems - US National Science Foundation (NSF)</gco:CharacterString>
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                            <gco:CharacterString>&lt;p&gt;&lt;strong&gt;Will Ocean Acidification Diminish Particle Aggregation and Mineral Scavenging, Thus Weakening the Biological Pump?&lt;/strong&gt;&lt;/p&gt;
&lt;p&gt;This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).&lt;/p&gt;
&lt;p&gt;The pH of the ocean is predicted to decrease by 0.2-0.5 pH units in the next 50 to100 years as a result of increasing atmospheric CO2. To date almost all the research on impending ocean acidification has focused on the impacts to calcifying organisms and the carbonate system. However, ocean acidification will also affect other significant marine processes that are pH dependent.&lt;/p&gt;
&lt;p&gt;In this project, researchers at the University of California at Santa Barbara will investigate the impact of ocean acidification on the organic carbon or 'soft tissue' biological pump. They predict that a decline in oceanic pH will result in an increase in the protonation of negatively charged substances, especially of Transparent Exopolymer Particles (TEP), the gel-like particles that provide the matrix of aggregates and bind particles together. A decreased polarity of these highly surface-active particles may reduce their &quot;stickiness&quot; resulting in decreased aggregation of organic-rich particles and a decreased ability of aggregates to scavenge and retain heavy ballast minerals. A reduction in aggregation will lower the fraction of POC enclosed in fast-sinking aggregates. Decreased scavenging of minerals by aggregates will result in reduced sinking velocities and consequently a decline in the fraction of material escaping degradation in the water column. Both processes ultimately reduce carbon flux to depth. The resulting weakening of the biological pump will alter pelagic ecology and potentially produce a positive feed-back pathway that further increases atmospheric CO2 concentrations.&lt;/p&gt;
&lt;p&gt;The research team will experimentally investigate TEP-production, aggregation rates and aggregate characteristics, mineral scavenging and sinking velocity as a function of ocean acidification, because these parameters are susceptible to pH and central in determining sedimentation rate of organic carbon. They will determine potential changes in the abiotic formation of TEP or in the release rate of TEP or TEP-precursors by phytoplankton that have been adapted to increased CO2 regimes for multiple generations, up to 1000 doublings. Additionally, they will experimentally test potential changes in the aggregation rate of adapted phytoplankton and natural particles, and measure impacts on scavenging rates of ballast minerals by aggregates. Effects of various acidification levels on aggregate characteristics, including size, composition, density, and sinking velocity will also be determined. These results are expected to provide parameterization for a predictive model that will be used to investigate the impact of changing ballasting or aggregation on carbon flux.&lt;/p&gt;
&lt;p&gt;Broader impact: Climate and environmental change are a global challenge to society. We need to know if possible positive feed back mechanisms to the biological pump will further increase atmospheric CO2 in order to prepare for and hopefully manage future climate changes.&lt;/p&gt;
&lt;p&gt;These data are also available at &lt;a href=&quot;http://doi.pangaea.de/10.1594/PANGAEA.778190&quot; target=&quot;_blank&quot;&gt;Pangea&lt;/a&gt;&lt;/p&gt;
&lt;p&gt;
&lt;strong&gt;RELATED FILES:&lt;/strong&gt;&lt;br /&gt;
Passow U (2012) The Abiotic Formation of Tep under Ocean Acidification Scenarios. Marine Chemistry 128-129:72-80&lt;/p&gt;
&lt;p&gt;
&lt;strong&gt;PUBLICATIONS PRODUCED AS A RESULT OF THIS RESEARCH&lt;/strong&gt;&lt;br /&gt;
Bathmann U, Passow U. &quot;Global Erwaermung. Kohlenstoffpumpen im Ozean steuern das Klima.,&quot; &lt;em&gt;Biologie in unserer Zeit 5&lt;/em&gt;, v.5, 2010.&lt;/p&gt;
&lt;p&gt;Benner I, Passow U. &quot;Utilization of organic nutrients by coccolithophores,&quot; &lt;em&gt;Marine Ecology Progress Series&lt;/em&gt;, v.404, 2010, p. 21.&lt;/p&gt;
&lt;p&gt;Feng Y, Hare C, Leblanc K, Rose J, Zhang Y, DiTullio G, Lee P, Wilhelm S, Rowe J, Sun J, Nemcek N, Gueguen C, Passow U, Benner I, Brown C, Hutchins D. &quot;Effects of increased pCO2 and temperature on the North Atlantic spring bloom. I. The phytoplankton community and biogeochemical response,&quot; &lt;em&gt;Marine Ecology Progress Series&lt;/em&gt;, v.388, 2009, p. 13.&lt;/p&gt;
&lt;p&gt;Gaerdes A, Iversen MH, Grossart H-P, Passow U, Ullrich M. &quot;Diatom associated bacteria are required for aggregation of Thalassiosira weissflogii.,&quot; &lt;em&gt;ISME Journal&lt;/em&gt;, 2010, p. 1.&lt;/p&gt;
&lt;p&gt;Leblanc K, Hare CE, Feng Y, Berg GM, DiTullio GR, Neeley A, Benner I, Sprengel C, Beck A, Sanudo-Wilhelmy SA, Passow U, Klinck K, Rowe JM, Wilhelm SW, Brown CW, Hutchins DA. &quot;Distribution of calcifying and silicifying phytoplankton in relation to environmental and biogeochemical parameters during the late stages of the 2005 North East Atlantic Spring Bloom,&quot; &lt;em&gt;Biogeosciences&lt;/em&gt;, v.6, 2009, p. 2155.&lt;/p&gt;
&lt;p&gt;Ploug H, Terbruggen A, Kaufmann A, Wolf-Gladrow D, Passow U. &quot;A novel method to measure particle sinking velocity in vitro, and its comparison to three other in vitro methods.,&quot; &lt;em&gt;Limnolgy and Oceanography Methods&lt;/em&gt;, v.8, 2010, p. 386.&lt;/p&gt;
&lt;p&gt;Passow, U., Rocha, C.L.D.L., Fairfield, C., Schmidt, K., 2014. Aggregation as a function of pCO2 and mineral particles. Limnology and Oceanography 59 (2), 532-547.&lt;/p&gt;
&lt;p&gt;De La Rocha, C.L., Passow, U., 2014. The biological pump. In: Turekian, K.K., Holland, H.D. (Eds.), Treatise on Geochemistry. Elsevier, Oxford, pp. 93-122.&lt;/p&gt;
&lt;p&gt;Boyd, P., Rynearson, T., Armstrong, E., Fu, F., Hayashi, K., Hu, Z., Hutchins, D., Kudela, R., Litchman, E., Mulholland, M., Passow, U., Strzepek, R., Whittaker, K., Yu, E., Thomas, M., 2013. Marine Phytoplankton Temperature versus Growth Responses from Polar to Tropical Waters - Outcome of a Scientific Community-Wide Study. PLoS ONE 8 (5), e63091.&lt;/p&gt;
&lt;p&gt;Passow, U., Carlson, C., 2012. The Biological Pump in a High CO2 World. Marine Ecology Progress Series 470, 249-271.&lt;br /&gt;
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http://lod.bco-dmo.org/id/dataset-parameter/34696.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/34697.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/34698.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/34699.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/34700.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/34701.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/34703.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/34707.rdf
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          </gmi:type>
          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: Nutrient Autoanalyzer - TRAACS CS800 autoanalyzer PI Supplied Instrument Description:DIC was measured colorimetrically in duplicate with a TRAACS CS800 autoanalyzer (Seal, Mequon, USA) with a precision of ± 5 μmol kg-1. CRM (Batch No. 54) supplied by A. Dickson was used as a calibration (see for methodological details). Instrument Name: Nutrient Autoanalyzer Instrument Short Name:Nutrient Autoanalyzer   Instrument Description: Nutrient Autoanalyzer is a generic term used when specific type, make and model were not specified.  In general, a Nutrient Autoanalyzer is an automated flow-thru system for doing nutrient analysis (nitrate, ammonium, orthophosphate, and silicate) on seawater samples. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB04/</gco:CharacterString>
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        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument>
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/528200.rdf" xlink:title="Roller Tank" xlink:actuate="onRequest">Roller Tank</gmx:Anchor>
              </gmd:code>
            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString>Roller Tank</gco:CharacterString>
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          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: Roller Tank PI Supplied Instrument Description:Roller tanks experiments: 14 cylindrical five-liter roller tanks/ treatments rotating at 1 rpm encompassing three acidification scenarios and 5 concentrations of the clay illite incubated at 14oC in continuous darkness for 44 to 48 hours. Solid body rotation was established within three hours. Instrument Name: Roller Tank Instrument Short Name:Roller Tank   Instrument Description: Rolling tanks, which keep particles in suspension, thus simulating aggregate formation in situ.

Marine snow experiments are conducted in roller tanks, which turn continuously, keeping marine snow in suspension. It is important for marine snow not to touch surfaces. The rolling tanks, which keep particles in suspension, thus simulate aggregate formation in situ. Marine snow formation due to different types of oil was tested. Some treatments are easily identifiable as containing oil by their color (middle). UCSB, CA 2012.</gco:CharacterString>
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        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument>
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/603.rdf" xlink:title="Shimadzu TOC-V Analyzer" xlink:actuate="onRequest">Shimadzu TOC-V</gmx:Anchor>
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            </gmd:MD_Identifier>
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          <gmi:type>
            <gco:CharacterString>Shimadzu TOC-V</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: Shimadzu TOC-V PI Supplied Instrument Description:The filtered samples were immediately frozen at -20°C and later analyzed via high temperature combustion on a modified Shimadzu TOC-V analyzers in the Carlson lab at UCSB. Instrument Name: Shimadzu TOC-V Analyzer Instrument Short Name:Shimadzu TOC-V   Instrument Description: A Shimadzu TOC-V Analyzer measures DOC by high temperature combustion method. Community Standard Description: http://onto.nerc.ac.uk/CAST/124</gco:CharacterString>
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        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument>
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/707.rdf" xlink:title="Spectrophotometer" xlink:actuate="onRequest">Genesys 10SVIS Spectrophotometer</gmx:Anchor>
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            <gco:CharacterString>Genesys 10SVIS Spectrophotometer</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: Genesys 10SVIS Spectrophotometer PI Supplied Instrument Description:The samples for pHT were collected bubble free in 20-ml scintillation vials and measured within 1-2 hours of sampling with a spectrophotometer (Genesys 10SVIS) equipped with a single cell Peltier (SPG1A, both Thermo Scientific) using the indicator dye m-cresol purple (Sigma-Aldrich) at a constant temperature of 25oC. Instrument Name: Spectrophotometer Instrument Short Name:Spectrophotometer   Instrument Description: An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/</gco:CharacterString>
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        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument>
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/682.rdf" xlink:title="Titrator" xlink:actuate="onRequest">Automatic titrator - TitroLine alpha plus (Schott Instruments, Mainz, Germany)</gmx:Anchor>
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            <gco:CharacterString>Automatic titrator - TitroLine alpha plus (Schott Instruments, Mainz, Germany)</gco:CharacterString>
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          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: Automatic titrator - TitroLine alpha plus (Schott Instruments, Mainz, Germany) PI Supplied Instrument Description:TA was calculated from linear Gran plots (Gran 1952) after duplicate potentiometric titration (Brewer et al. 1986) using a TitroLine alpha plus (Schott Instruments, Mainz, Germany). Average precision was ± 5 μmol kg-1. Certified Reference Materials (CRMs, Batch No. 54) supplied by A. Dickson (Scripps Institution of Oceanography, USA) was used as a control. Instrument Name: Titrator Instrument Short Name:Titrator   Instrument Description: Titrators are instruments that incrementally add quantified aliquots of a reagent to a sample until the end-point of a chemical reaction is reached. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB12/</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:operation>
          <gmi:MI_Operation>
            <gmi:description>
              <gco:CharacterString>Deployment: lab_UCSB_MSI_Passow</gco:CharacterString>
            </gmi:description>
            <gmi:identifier>
              <gmd:MD_Identifier>
                <gmd:code>
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            </gmi:identifier>
            <gmi:status>
              <gmd:MD_ProgressCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#MD_ProgressCode" codeListValue="completed"/>
            </gmi:status>
            <gmi:type>
              <gmi:MI_OperationTypeCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#MI_OperationTypeCode" codeListValue="real"/>
            </gmi:type>
            <gmi:parentOperation gco:nilReason="inapplicable"/>
            <gmi:platform>
  <gmi:MI_Platform>
    <gmi:citation>
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         <gmx:Anchor xlink:href="http://www.msi.ucsb.edu/research/research-labs-msi/passow-lab" xlink:actuate="onRequest">UCSB MSI Passow</gmx:Anchor>
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    </gmi:citation>
    <gmi:identifier>
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        <gmd:code>
          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/platform/54202.rdf"
           xlink:actuate="onRequest">UCSB MSI Passow</gmx:Anchor>
        </gmd:code>
      </gmd:MD_Identifier>
    </gmi:identifier>
    <gmi:description>
      <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/platform/54202.rdf" xlink:title="UCSB MSI Passow" xlink:actuate="onRequest">laboratory</gmx:Anchor>
    </gmi:description>
    <gmi:instrument gco:nilReason="unknown"/>
  </gmi:MI_Platform>
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            <gmi:plan>
              <gmi:MI_Plan>
                <gmi:status>
                  <gmd:MD_ProgressCode codeList="http://www.isotc211.org/2005/resources/Codelist/gmxCodelists.xml#MD_ProgressCode" codeListValue="completed"/>
                </gmi:status>
                <gmi:citation>
                  <gmd:CI_Citation>
                    <gmd:title>
                      <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/deployment/58780.rdf" xlink:title="Deployment" xlink:actuate="onRequest">lab_UCSB_MSI_Passow</gmx:Anchor>
                    </gmd:title>
                    <gmd:date gco:nilReason="unknown"/>
                    <gmd:citedResponsibleParty>
                      <gmd:CI_ResponsibleParty>
                      <gmd:individualName>
                          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/person/51317.rdf" xlink:actuate="onRequest">Uta Passow</gmx:Anchor>
                        </gmd:individualName>
                        <gmd:organisationName>
                          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/affiliation/234.rdf" xlink:title="Affiliation" xlink:actuate="onRequest">University of California-Santa Barbara</gmx:Anchor>
                        </gmd:organisationName>
                        <gmd:contactInfo>
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                          <gmd:onlineResource>
                            <gmd:CI_OnlineResource>
                              <gmd:linkage>
                                <gmd:URL>http://www.msi.ucsb.edu/people/research-scientists/uta-passow</gmd:URL>
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                              <gmd:description>
                                <gco:CharacterString>Report describing lab_UCSB_MSI_Passow</gco:CharacterString>
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                          </gmd:onlineResource>
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                      <gmd:role>
                        <gmd:CI_RoleCode codeList="http://www.isotc211.org/2005/resources/Codelist/gmxCodelists.xml#CI_RoleCode" codeListValue="principalInvestigator"/>
                      </gmd:role>
                      </gmd:CI_ResponsibleParty>
                    </gmd:citedResponsibleParty>
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                </gmi:citation>
              </gmi:MI_Plan>
            </gmi:plan>
            </gmi:MI_Operation>
      </gmi:operation><gmi:platform>
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    <gmi:citation>
     <gmd:CI_Citation>
       <gmd:title>
         <gmx:Anchor xlink:href="http://www.msi.ucsb.edu/research/research-labs-msi/passow-lab" xlink:actuate="onRequest">UCSB MSI Passow</gmx:Anchor>
       </gmd:title>
       <gmd:date gco:nilReason="unknown"/>
     </gmd:CI_Citation>
    </gmi:citation>
    <gmi:identifier>
      <gmd:MD_Identifier>
        <gmd:code>
          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/platform/54202.rdf"
           xlink:actuate="onRequest">UCSB MSI Passow</gmx:Anchor>
        </gmd:code>
      </gmd:MD_Identifier>
    </gmi:identifier>
    <gmi:description>
      <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/platform/54202.rdf" xlink:title="UCSB MSI Passow" xlink:actuate="onRequest">laboratory</gmx:Anchor>
    </gmi:description>
    <gmi:instrument gco:nilReason="unknown"/>
  </gmi:MI_Platform>
</gmi:platform>
          </gmi:MI_AcquisitionInformation>
  </gmi:acquisitionInformation>
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