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            <gco:CharacterString>Cite this dataset as: Lomas, M. (2013) Biogeochemical and biological data from Niskin bottle samples from R/V Atlantic Explorer cruises AE1102, AE1118, AE1206, AE1219 in the Sargasso Sea, Bermuda Atlantic Time-Series Station from 2011-2012 (Trophic BATS project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2013-05-21 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.3951.1 [access date]</gco:CharacterString>
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        <gco:CharacterString>Biogeochemical and biological data from the Sargasso Sea. Dataset Description: &amp;lt;p&amp;gt;Biogeochemical and biological data collected on the Trophic BATS cruises in the Sargasso Sea. Data are from 4 cruises over the span 2011-2012. The provided data are complete matrices and therefore not every sample (columns) will be taken from every Niskin fired (rows).&amp;lt;/p&amp;gt; Methods and Sampling: &amp;lt;p&amp;gt;Methods are summarized below. Detailed methods for all data collected as part of this study can be found in the publications arising from this study (references given below).&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;On each BATS cruise, aquasi-lagrangian sampling scheme is employed. An in situ primary productivity array is deployed from dawn to dusk. The biogeochemistry and biological parameters reported in this data were measured from Niskin bottle water samples.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Bacterial production was measured using [3H-methyl] thymidine incorporation and converted to carbon-based bacterial production using standard equations. Bacterial abundance was determined using DAPI stained epifluorescence microscopy. Pico-autotrophs were identified as either Synechococcus and Prochlorococcus.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Samples for NO3/NO2, NO2 and PO4 are filtered and frozen (-20 degrees C) in HDPE bottles until analysis. Total organic carbon (TOC) and total nitrogen were determined using high temperature combustion techniques. Total phosphorus concentrations are quantified using a high temperature/persulfateoxidation technique. Particulate organic carbon (POC) and nitrogen (PON) samples were filtered on precombusted Whatman GF/F filters and frozen until analysis on an elemental analyzer. Particulate phosphorus samples were analyzed using an ash-hydrolysis method with oxidation efficiency and standard recovery checks.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Sample QA/QC procedures followed those given in the associated manuscripts. At the point of collection, any leaking niskin bottles were noted on the master cast sheets and samples were taken from a different niskin fired at the same depth as the leaking bottle. No data are reported for leaking Niskin bottles. During sample analysis, certified standards, where available, were carefully examined to ensure that they were consistent with expectations for accuracy and precision. If no obvious error or problem was found, the data were considered OK and in the range of environmental data that this study hoped to observe.&amp;lt;br /&amp;gt;
&amp;lt;br /&amp;gt;
Sample accuracy was assessed by using certified standards, for those measurements where standards are available. Certified standards were run with each analytical run and compared to long term control charts for respective analyses. For those analyses where there are no standards (e.g., flow cytometric cell counts) data were assessed for reasonableness based upon extensive experience of the PI’s.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Detailed information on analyses:&amp;lt;br /&amp;gt;
Lomas, M.W., Burke, A., Lomas, D.A., Bell, D.W., Shen, C., Ammerman, J.W., Dyhrman, S.T. 2010.&amp;amp;nbsp; Sargasso Sea phosphorus biogeochemistry: An important role for dissolved organic phosphorus (DOP). Biogeosciences 7: 695-710. doi: &amp;lt;a href=&amp;quot;http://dx.doi.org/10.5194/bg-7-695-2010&amp;quot; target=&amp;quot;_blank&amp;quot;&amp;gt;10.5194/bg-7-695-2010&amp;lt;/a&amp;gt;&amp;lt;br /&amp;gt;
Lomas, M.W., Bates, N.R., Johnson, R.J., Knap, A.H., Steinberg, D.K., Carlson, C.A. 2013. Two decades and counting: overview of 24-years of sustained open ocean biogeochemical measurements. Deep Sea Research II doi: &amp;lt;a href=&amp;quot;http://dx.doi.org/10.1016/j.dsr2.2013.01.008&amp;quot; target=&amp;quot;_blank&amp;quot;&amp;gt;10.1016/j.dsr2.2013.01.008&amp;lt;/a&amp;gt;.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;References:&amp;lt;br /&amp;gt;
Casey, J.R., Aucan, J.P., Goldberg, S.R., and Lomas, M.W. 2013. Changes in partitioning of carbon amongst photosynthetic pico- and nano-plankton groups in the Sargasso Sea in response to changes in the North Atlantic Oscillation. Deep Sea Research II doi: &amp;lt;a href=&amp;quot;http://dx.doi.org/10.1016/j.dsr2.2013.02.002&amp;quot; target=&amp;quot;_blank&amp;quot;&amp;gt;10.1016/j.dsr2.2013.02.002&amp;lt;/a&amp;gt;&amp;lt;/p&amp;gt;</gco:CharacterString>
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                  <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/project/2150.rdf" xlink:title="Project" xlink:actuate="onRequest">Plankton Community Composition and Trophic Interactions as Modifiers of Carbon Export in the Sargasso Sea</gmx:Anchor>
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                            <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/project/2150.rdf" xlink:title="Project Name" xlink:actuate="onRequest">Plankton Community Composition and Trophic Interactions as Modifiers of Carbon Export in the Sargasso Sea</gmx:Anchor>
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                            <gco:CharacterString>&lt;p&gt;Fluxes of particulate carbon from the surface ocean are greatly influenced by the size, taxonomic composition and trophic interactions of the resident planktonic community. Large and/or heavily-ballasted phytoplankton such as diatoms and coccolithophores are key contributors to carbon export due to their high sinking rates and direct routes of export through large zooplankton. The potential contributions of small, unballasted phytoplankton, through aggregation and/or trophic re-packaging, have been recognized more recently. This recognition comes as direct observations in the field show unexpected trends. In the Sargasso Sea, for example, shallow carbon export has increased in the last decade but the corresponding shift in phytoplankton community composition during this time has not been towards larger cells like diatoms. Instead, the abundance of the picoplanktonic cyanobacterium, Synechococccus, has increased significantly. The trophic pathways that link the increased abundance of Synechococcus to carbon export have not been characterized. These observations helped to frame the overarching research question, &quot;How do plankton size, community composition and trophic interactions modify carbon export from the euphotic zone&quot;. Since small phytoplankton are responsible for the majority of primary production in oligotrophic subtropical gyres, the trophic interactions that include them must be characterized in order to achieve a mechanistic understanding of the function of the biological pump in the oligotrophic regions of the ocean.&lt;/p&gt;
&lt;p&gt;This requires a complete characterization of the major organisms and their rates of production and consumption. Accordingly, the research objectives are: 1) to characterize (qualitatively and quantitatively) trophic interactions between major plankton groups in the euphotic zone and rates of, and contributors to, carbon export and 2) to develop a constrained food web model, based on these data, that will allow us to better understand current and predict near-future patterns in export production in the Sargasso Sea.&lt;/p&gt;
&lt;p&gt;The investigators will use a combination of field-based process studies and food web modeling to quantify rates of carbon exchange between key components of the ecosystem at the Bermuda Atlantic Time-series Study (BATS) site. Measurements will include a novel DNA-based approach to characterizing and quantifying planktonic contributors to carbon export. The well-documented seasonal variability at BATS and the occurrence of mesoscale eddies will be used as a natural laboratory in which to study ecosystems of different structure. This study is unique in that it aims to characterize multiple food web interactions and carbon export simultaneously and over similar time and space scales. A key strength of the proposed research is also the tight connection and feedback between the data collection and modeling components.&lt;/p&gt;
&lt;p&gt;Characterizing the complex interactions between the biological community and export production is critical for predicting changes in phytoplankton species dominance, trophic relationships and export production that might occur under scenarios of climate-related changes in ocean circulation and mixing. The results from this research may also contribute to understanding of the biological mechanisms that drive current regional to basin scale variability in carbon export in oligotrophic gyres.&lt;/p&gt;</gco:CharacterString>
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                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/project/2150.rdf" xlink:title="Project Acronym" xlink:actuate="onRequest">Trophic BATS</gmx:Anchor>
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            <gco:CharacterString>BCO-DMO catalogue of parameters from Biogeochemical and biological data from Niskin bottle samples from R/V Atlantic Explorer cruises AE1102, AE1118, AE1206, AE1219 in the Sargasso Sea, Bermuda Atlantic Time-Series Station from 2011-2012 (Trophic BATS project)</gco:CharacterString>
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            http://lod.bco-dmo.org/id/dataset-parameter/34777.rdf
	Name: cruise_id
	Units: text
	Description: &lt;p&gt;Official cruise identifier e.g. AE1102 = R/V Atlantic Explorer cruise number 1102.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/34778.rdf
	Name: date
	Units: unitless
	Description: &lt;p&gt;Date of operation in mm/dd/yyyy format.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/34779.rdf
	Name: cast
	Units: integer
	Description: &lt;p&gt;CTD drop number.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/34780.rdf
	Name: station
	Units: integer or text
	Description: &lt;p&gt;Station number/name.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/34781.rdf
	Name: lat
	Units: decimal degree
	Description: &lt;p&gt;Latitude; positive is North.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/34782.rdf
	Name: lon
	Units: decimal degree
	Description: &lt;p&gt;Longitude; positive is East.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/34783.rdf
	Name: julian_day
	Units: decimal day
	Description: &lt;p&gt;Julian day.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/34784.rdf
	Name: time_local
	Units: HHMM
	Description: &lt;p&gt;Time (local).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/34785.rdf
	Name: depth
	Units: meters
	Description: &lt;p&gt;Actual depth of niskin fire.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/34786.rdf
	Name: depth_nom
	Units: meters
	Description: &lt;p&gt;Target depth of niskin fire.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/34787.rdf
	Name: niskin_flag
	Units: dimensionless
	Description: &lt;p&gt;Quality flag for the niskin bottle fire.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/34788.rdf
	Name: temp
	Units: degrees Celsius
	Description: &lt;p&gt;Temperature measured by CTD.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/34789.rdf
	Name: sal
	Units: parts per thousand (ppt)
	Description: &lt;p&gt;Salinity measured by CTD.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/34790.rdf
	Name: density
	Units: kg per cubic meter
	Description: &lt;p&gt;Density measured by CTD (kg m-3).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/34791.rdf
	Name: chl_a
	Units: micrograms per liter
	Description: &lt;p&gt;Chlorohyll-a measured by CTD (ug/L).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/34792.rdf
	Name: O2
	Units: micromoles per kilogram
	Description: &lt;p&gt;O2 measured by CTD (umol/kg).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/34793.rdf
	Name: beam
	Units: reciprocal meters
	Description: &lt;p&gt;Beam attenuation (1/m).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/34794.rdf
	Name: chla_tot_whole
	Units: micrograms per liter
	Description: &lt;p&gt;Total chlorophyll-a (ug/L).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/34795.rdf
	Name: chla_tot_gt5um
	Units: micrograms per liter
	Description: &lt;p&gt;Chlorophyll-a (ug/L); fraction greater than 5 um.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/34796.rdf
	Name: bact_prod
	Units: pmol Thy per liter per hour
	Description: &lt;p&gt;Small volume bacterial production measured by thymidine incorporation (pmol Thy L-1 h-1)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/34797.rdf
	Name: bact_prod_C
	Units: milligrams C per cubic meter per day
	Description: &lt;p&gt;Bacterial thymidine production converted to C units using conversions in Carlson et al. 1996 (mgC m-3 d-1).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/34798.rdf
	Name: bact_abund
	Units: cells per milliliter
	Description: &lt;p&gt;Bacterial abundance by DAPI staining and epifluorescent counting (cells/mL).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/34799.rdf
	Name: bact_POC
	Units: micrograms per liter
	Description: &lt;p&gt;Bacterial abundance converted to C units using factors in Carlson et al. 1996 (ug/L).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/34800.rdf
	Name: prochlorococcus
	Units: cells per milliliter
	Description: &lt;p&gt;Prochlorococcus abundance by flow cytometry (cells/mL).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/34801.rdf
	Name: synechococcus
	Units: cells per milliliter
	Description: &lt;p&gt;Synechococcus abundance by flow cytometry (cells/mL).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/34802.rdf
	Name: peuks
	Units: cells per milliliter
	Description: &lt;p&gt;Picoeukaryote abundance by flow cytometry (cells/mL).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/34803.rdf
	Name: neuks
	Units: cells per milliliter
	Description: &lt;p&gt;Nanoeukaryote abundance by flow cytometry (cells/mL).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/34804.rdf
	Name: prochlor_POC_per_cell
	Units: femtograms C per cell
	Description: &lt;p&gt;Average particulate organic carbon (POC) content of Prochlorococcus cells derived from POC vs. flow cytometry based forward angle light scatter (Casey et al. 2013); fg/cell.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/34805.rdf
	Name: synecho_POC_per_cell
	Units: femtograms C per cell
	Description: &lt;p&gt;Average particulate organic carbon (POC) content of Synechococcus cells derived from POC vs. flow cytometry based forward angle light scatter (Casey et al. 2013); fg/cell&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/34806.rdf
	Name: peuks_POC_per_cell
	Units: femtograms C per cell
	Description: &lt;p&gt;Average particulate organic carbon (POC) content of picoeukaryotes derived from POC vs. flow cytometry based forward angle light scatter (Casey et al. 2013); fg/cell.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/34807.rdf
	Name: neuks_POC_per_cell
	Units: femtograms C per cell
	Description: &lt;p&gt;Average particulate organic carbon (POC) content of nanoeukaryotes derived from POC vs. flow cytometry based forward angle light scatter (Casey et al. 2013); fg/cell.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/34808.rdf
	Name: prochlor_POC
	Units: micromoles per liter
	Description: &lt;p&gt;POC (umol/L) for the entire Prochlorococcus population, calculated as POC per cell times cell abundance.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/34809.rdf
	Name: synecho_POC
	Units: micromoles per liter
	Description: &lt;p&gt;POC (umol/L) for the entire Synechococcus population, calculated as POC per cell times cell abundance.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/34810.rdf
	Name: peuks_POC
	Units: micromoles per liter
	Description: &lt;p&gt;POC (umol/L) for the entire picoeukaryote population, calculated as POC per cell times cell abundance.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/34811.rdf
	Name: neuks_POC
	Units: micromoles per liter
	Description: &lt;p&gt;POC (umol/L)  for the entire nanoeukaryote population, calculated as POC per cell times cell abundance.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/34812.rdf
	Name: NO3_NO2
	Units: micromoles per liter
	Description: &lt;p&gt;Combined nitrate and nitrite concentrations by AutoAnalyzer (umol/L).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/34813.rdf
	Name: NO2
	Units: micromoles per liter
	Description: &lt;p&gt;Nitrite concentration by AutoAnalyzer (umol/L).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/34814.rdf
	Name: PO4
	Units: micromoles per liter
	Description: &lt;p&gt;Phosphate concentration by AutoAnalyzer (umol/L).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/34815.rdf
	Name: SiOH4
	Units: micromoles per liter
	Description: &lt;p&gt;Silicate concentration by AutoAnalyzer (umol/L).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/34816.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/34817.rdf
	Name: POC
	Units: micromoles per liter
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http://lod.bco-dmo.org/id/dataset-parameter/34818.rdf
	Name: PON
	Units: micromoles per liter
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http://lod.bco-dmo.org/id/dataset-parameter/34819.rdf
	Name: POP
	Units: micromoles per liter
	Description: &lt;p&gt;Particulate organic phosphorus concentration (umol/L).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/34820.rdf
	Name: TOC
	Units: micromoles per liter
	Description: &lt;p&gt;Total organic carbon concentration (umol/L).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/34821.rdf
	Name: TON
	Units: micromoles per liter
	Description: &lt;p&gt;Total organic nitrogen concentration (umol/L).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/34822.rdf
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	Units: micromoles per liter
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&amp;lt;p&amp;gt;On each BATS cruise, aquasi-lagrangian sampling scheme is employed. An in situ primary productivity array is deployed from dawn to dusk. The biogeochemistry and biological parameters reported in this data were measured from Niskin bottle water samples.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Bacterial production was measured using [3H-methyl] thymidine incorporation and converted to carbon-based bacterial production using standard equations. Bacterial abundance was determined using DAPI stained epifluorescence microscopy. Pico-autotrophs were identified as either Synechococcus and Prochlorococcus.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Samples for NO3/NO2, NO2 and PO4 are filtered and frozen (-20 degrees C) in HDPE bottles until analysis. Total organic carbon (TOC) and total nitrogen were determined using high temperature combustion techniques. Total phosphorus concentrations are quantified using a high temperature/persulfateoxidation technique. Particulate organic carbon (POC) and nitrogen (PON) samples were filtered on precombusted Whatman GF/F filters and frozen until analysis on an elemental analyzer. Particulate phosphorus samples were analyzed using an ash-hydrolysis method with oxidation efficiency and standard recovery checks.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Sample QA/QC procedures followed those given in the associated manuscripts. At the point of collection, any leaking niskin bottles were noted on the master cast sheets and samples were taken from a different niskin fired at the same depth as the leaking bottle. No data are reported for leaking Niskin bottles. During sample analysis, certified standards, where available, were carefully examined to ensure that they were consistent with expectations for accuracy and precision. If no obvious error or problem was found, the data were considered OK and in the range of environmental data that this study hoped to observe.&amp;lt;br /&amp;gt;
&amp;lt;br /&amp;gt;
Sample accuracy was assessed by using certified standards, for those measurements where standards are available. Certified standards were run with each analytical run and compared to long term control charts for respective analyses. For those analyses where there are no standards (e.g., flow cytometric cell counts) data were assessed for reasonableness based upon extensive experience of the PI’s.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Detailed information on analyses:&amp;lt;br /&amp;gt;
Lomas, M.W., Burke, A., Lomas, D.A., Bell, D.W., Shen, C., Ammerman, J.W., Dyhrman, S.T. 2010.&amp;amp;nbsp; Sargasso Sea phosphorus biogeochemistry: An important role for dissolved organic phosphorus (DOP). Biogeosciences 7: 695-710. doi: &amp;lt;a href=&amp;quot;http://dx.doi.org/10.5194/bg-7-695-2010&amp;quot; target=&amp;quot;_blank&amp;quot;&amp;gt;10.5194/bg-7-695-2010&amp;lt;/a&amp;gt;&amp;lt;br /&amp;gt;
Lomas, M.W., Bates, N.R., Johnson, R.J., Knap, A.H., Steinberg, D.K., Carlson, C.A. 2013. Two decades and counting: overview of 24-years of sustained open ocean biogeochemical measurements. Deep Sea Research II doi: &amp;lt;a href=&amp;quot;http://dx.doi.org/10.1016/j.dsr2.2013.01.008&amp;quot; target=&amp;quot;_blank&amp;quot;&amp;gt;10.1016/j.dsr2.2013.01.008&amp;lt;/a&amp;gt;.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;References:&amp;lt;br /&amp;gt;
Casey, J.R., Aucan, J.P., Goldberg, S.R., and Lomas, M.W. 2013. Changes in partitioning of carbon amongst photosynthetic pico- and nano-plankton groups in the Sargasso Sea in response to changes in the North Atlantic Oscillation. Deep Sea Research II doi: &amp;lt;a href=&amp;quot;http://dx.doi.org/10.1016/j.dsr2.2013.02.002&amp;quot; target=&amp;quot;_blank&amp;quot;&amp;gt;10.1016/j.dsr2.2013.02.002&amp;lt;/a&amp;gt;&amp;lt;/p&amp;gt;</gco:CharacterString>
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&amp;lt;br /&amp;gt;
Only nutrient analyses were close to analytical method detection limits (MDL). MDLs were estimated as 3x the standard deviation of the lowest standard used for the analysis and are ~30nM for nitrate and phosphate using a standard autoanalyzer. We used the MAGIC co-precipitation method for phosphate which lowered our MDL to ~0.5nM. Samples below the MDL are reported as the MDL.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;BCO-DMO Processing Notes:&amp;lt;br /&amp;gt;
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