http://lod.bco-dmo.org/id/dataset/3966
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2013-06-13
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Results of laboratory experiments examining Cyanobacteria (Trichodesmium and Crocosphaera watsonii) N2-fixation responses to pCO2; conducted in the Hutchins Laboratory, USC
2013-06-13
publication
2013-06-13
revision
BCO-DMO Linked Data URI
2013-06-13
creation
http://lod.bco-dmo.org/id/dataset/3966
David A. Hutchins
University of Southern California
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Hutchins, D. A. (2013) Results of laboratory experiments examining Cyanobacteria (Trichodesmium and Crocosphaera watsonii) N2-fixation responses to pCO2; conducted in the Hutchins Laboratory, USC. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 13 June 2013) Version Date 2013-06-13 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/3966 [access date]
Cyanobacteria (Trichodesmium and Crocosphaera watsonii) N2-fixation responses to pCO2. Dataset Description: <p>Cyanobacteria N2 fixation responses to pCO2 using four <em>Trichodesmium</em> isolates representing three species and three <em>Crocosphaera watsonii </em>strains, isolated from locations across the North and South Pacific and Atlantic Oceans (Webb et al. 2009, Hynes et al. 2012) and spanning the taxonomic diversity within each genus.</p>
<p>Detailed methods and results will be available in the following publication (see Figures 1 and 2):<br />
Hutchins, D.A., Fu, F.-X., Webb, E.A. and Tagliabue, A.&nbsp; (In press). Taxon-specific response of marine nitrogen fixers to elevated carbon dioxide concentrations. Nature Geoscience.</p> Methods and Sampling: <p>The seven isolates were grown across a pCO2 range in triplicate steady-state semi-continuous cultures at 28 degrees C on a 12 h dark:12 h light cycle using cool white fluorescent bulbs at 120 µmol photons m-2 s-1, in 0.2 µm-filtered, microwave-sterilized artificial seawater enriched with 20 µM phosphate and Aquil trace metals and vitamins, but with no fixed nitrogen (Hutchins et al. 2007, Fu et al. 2008). Cultures were diluted every 2-3 days based on their growth rates, and were sampled when acclimation to experimental conditions was verified by statistically invariant growth rates over 10-15 generations. For <em>Trichodesmium</em> KO4-20, H9-4, and 2174 and <em>Crocosphaera</em> WH0401, WH0003 and WH8501 experimental CO2 concentrations were 100ppm, 190ppm, 280ppm, 750ppm, 1500ppm, and 2000ppm. The CO2 response curve for <em>Trichodesmium</em> GBR was obtained from data from a previous experiment (Hutchins et al. 2007) at concentrations of 150ppm, 370ppm, 750ppm, 1250ppm and 1500 ppm. Gentle bubbling with certified commercial air/CO2 mixtures (Praxair) was used to obtain these seawater concentrations, which were verified by dissolved inorganic carbon (DIC) and pH measurements (Hutchins et al. 2007, Fu et al. 2008). Triplicate preserved 25 mL DIC samples (200 µL 5% HgCl2) were stored in borosilicate flasks at 4 degrees C for &lt;7 days until analysis. Total DIC was measured coulomb-metrically (model CM 140, UIC, Joliet, IL, USA) and pH was monitored daily using a microprocessor pH meter (NBS system) with three point buffer calibrations. Certified pCO2 values were verified using measured DIC and pH values with CO2 SYS software (<a href="http://cdiac.ornl.gov/ftp/co2sys/" target="_blank">http://cdiac.ornl.gov/ftp/co2sys/</a>). Since measured values never deviated &gt;1-2% from commercial certified values, pCO2 is reported as the latter value.</p>
<p>Rates of N2 fixation were measured at the same time of day for each culture (during the dark period for <em>Crocosphaera </em>and light period for <em>Trichodesmium</em>) with the acetylene reduction method using a 3:1 ratio to convert ethylene production to N2 fixation, and were normalized to culture chlorophyll a levels. Microscope counts were used to calculate cell-specific exponential growth rates (µ)( N<sub>T</sub>=N<sub>0</sub>e<sup>uT</sup>, where N is the initial cell density, N<sub>T</sub> is the cell density one day later, and T is one day) (Hutchins et al. 2007, Fu et al. 2008).</p>
<p>CO2 response curves for N2 fixation rates in each of the triplicate cultures for each isolate in each pCO2 treatment were fitted to Michaelis-Menten (1913) rectangular hyperbolic saturation equation curves (N2 fixation rate = Vmax * pCO2 / (K1/2 + pCO2)) using SigmaPlot software (SPSS), including determination of kinetic constants and curve correlation coefficients. Means and standard deviations of K1/2 (ppmv CO2) and Vmax (µmol N mg Chl a-1 h-1) values from the triplicate response curves are reported; significance of differences in K1/2 and Vmax values between isolates were tested using one-way ANOVA, followed by Fisher's Least Significant Difference to compare the mean of one group with the mean of another with SPSS statistics software (Hutchins et al. 2007, Fu et al. 2008).&nbsp; Multi-variate Principle Coordinate Analysis (PCoA) and Hierarchical Clustering were used to analyze the variance between all treatments and replicates in order to group them using their K1/2 and Vmax values as metrics of their responses to the CO2 treatments (Ramette 2007).</p>
<p><strong>References</strong><br />
Fu, F.-X., Mulholland, M.R., Garcia, N., Beck, A., Bernhardt, P.W., Warner, M.E., Sañudo-Wilhelmy, S.A. and Hutchins, D.A.&nbsp; 2008. Interactions between changing pCO2, N2 fixation, and Fe limitation in the marine unicellular cyanobacterium Crocosphaera. Limnology and Oceanography 53 (6): 2472- 2484. DOI: <a href="http://dx.doi.org/10.4319/lo.2008.53.6.2472" target="_blank">10.4319/lo.2008.53.6.2472</a></p>
<p>Hynes, A.M., Webb, E.A., Doney, S.C., and Waterbury, J.B. 2012. Comparison of cultured Trichodesmium (Cyanophyceae) with species characterized from the field. Journal of Phycology 48: 196-210. DOI:&nbsp;<a href="http://dx.doi.org/10.1111/j.1529-8817.2011.01096.x" target="_blank">10.1111/j.1529-8817.2011.01096.x</a></p>
<p>Hutchins, D. A., Fu, F.-X., Zhang, Y., Warner, M. E., Feng, Y. et al.&nbsp; 2007. CO2 control of Trichodesmium N2 fixation, photosynthesis, growth rates, and elemental ratios:&nbsp; Implications for past, present, and future ocean biogeochemistry. Limnology and Oceanography 52: 1293-1304. DOI: <a href="http://dx.doi.org/10.4319/lo.2007.52.4.1293" target="_blank">10.4319/lo.2007.52.4.1293</a></p>
<p>Michaelis, L., and Menten, M. M. 1913. Die Kinetik der Invertinwirkung. Biochem. Z. 49: 333–369.</p>
<p>Ramette, Alban. 2007. Multivariate analyses in microbial ecology. FEMS Microbiol. Ecol. 62: 142–160. DOI:&nbsp;<a href="http://dx.doi.org/10.1111/j.1574-6941.2007.00375.x" target="_blank">10.1111/j.1574-6941.2007.00375.x</a></p>
<p>Webb, E. A., Ehrenreich, I. M., Brown, S. L., Valois, F. W., and Waterbury, J. B. 2009. Phenotypic and genotypic characterization of multiple strains of the diazotrophic cyanobacterium, <em>Crocosphaera watsonii</em>, isolated from the open ocean. Environmental Microbiology 11:338-348. DOI:&nbsp;<a href="http://dx.doi.org/10.1111/j.1462-2920.2008.01771.x" target="_blank">10.1111/j.1462-2920.2008.01771.x</a></p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0722337 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0722337
completed
David A. Hutchins
University of Southern California
213-740-5616
Department of Biological Sciences 3616 Trousdale Parkway, AHF 20
Los Angeles
CA
90089
USA
dahutch@usc.edu
pointOfContact
asNeeded
Dataset Version: 13 June 2013
Unknown
isolate
s_to_v_ratio
half_sat
half_sat_sd
vmax
vmax_sd
pCO2
N2_fixation
N2_fixation_sd
Benchtop pH Meter
CO2 Coulometer
theme
None, User defined
taxon
No BCO-DMO term
standard deviation
Partial pressure of CO2
featureType
BCO-DMO Standard Parameters
Benchtop pH Meter
CO2 Coulometer
instrument
BCO-DMO Standard Instruments
lab_Hutchins_07-12_diazotrophs
service
Deployment Activity
Hutchins Laboratory, University of Southern California
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
CO2 control of oceanic nitrogen fixation and carbon flow through diazotrophs
https://osprey.bco-dmo.org/project/2172
CO2 control of oceanic nitrogen fixation and carbon flow through diazotrophs
<p><i>From NSF award abstract:</i></p>
<p>The importance of marine N2 fixation to present ocean productivity and global nutrient and carbon biogeochemistry is now universally recognized. Marine N2 fixation rates and oceanic N inventories are also thought to have varied over geological time due to climate variability and change. However, almost nothing is known about the responses of dominant N2 fixers in the ocean such as Trichodesmium and unicellular N2 fixing cyanobacteria to past, present and future global atmospheric CO2 regimes. Our preliminary data demonstrate that N2 and CO2 fixation rates, growth rates, and elemental ratios of Atlantic and Pacific Trichodesmium isolates are controlled by the ambient CO2 concentration at which they are grown. At projected year 2100 pCO2 (750 ppm), N2 fixation rates of both strains increased 35-100%, with simultaneous increases in C fixation rates and cellular N:P and C:P ratios. Surprisingly, these increases in N2 and C fixation due to elevated CO2 were of similar relative magnitude regardless of the growth temperature or P availability. Thus, the influence of CO2 appears to be independent of other common growth-limiting factors. Equally important, Trichodesmium growth and N2 fixation were completely halted at low pCO2 levels (150 ppm), suggesting that diazotrophy by this genus may have been marginal at best at last glacial maximum pCO2 levels of ~190 ppm. Genetic evidence indicates that Trichodesmium diazotrophy is subject to CO2 control because this cyanobacterium lacks high-affinity dissolved inorganic carbon transport capabilities. These findings may force a re-evaluation of the hypothesized role of past marine N2 fixation in glacial/interglacial climate changes, as well as consideration of the potential for increased ocean diazotrophy and altered nutrient and carbon cycling in the future high-CO2 ocean.</p>
<p>We propose an interdisciplinary project to examine the relationship between ocean N2 fixing cyanobacteria and changing pCO2. A combined field and laboratory approach will incorporate in situ measurements with experimental manipulations using natural and cultured populations of Trichodesmium and unicellular N2 fixers over range of pCO2 spanning glacial era to future concentrations (150-1500 ppm). We will also examine how effects of pCO2 on N2 and C fixation and elemental stoichiometry are moderated by the availability of other potentially growth-limiting variables such as Fe, P, temperature, and light. We plan to obtain a detailed picture of the full range of responses of important oceanic diazotrophs to changing pCO2, including growth rates, N2 and CO2 fixation, cellular elemental ratios, fixed N release, photosynthetic physiology, and expression of key genes involved in carbon and nitrogen acquisition at both the transcript and protein level.</p>
<p>This research has the potential to evolutionize our understanding of controls on N2 fixation in the ocean. Many of our current ideas about the interactions between oceanic N2 fixation, atmospheric CO2, nutrient biogeochemistry, ocean productivity, and global climate change may need revision to take into account previously unrecognized feedback mechanisms between atmospheric composition and diazotrophs. Our findings could thus have major implications for human society, and its increasing dependence on ocean resources in an uncertain future. This project will take the first vital steps towards understanding how a biogeochemically-critical process, the fixation of N2 in the ocean, may respond to our rapidly changing world during the century to come.</p>
Diaz N2-Fix in High CO2
largerWorkCitation
project
eng; USA
oceans
Hutchins Laboratory, University of Southern California
2013-06-13
Laboratory
0
BCO-DMO catalogue of parameters from Results of laboratory experiments examining Cyanobacteria (Trichodesmium and Crocosphaera watsonii) N2-fixation responses to pCO2; conducted in the Hutchins Laboratory, USC
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/35213.rdf
Name: isolate
Units: text
Description: <p>Name of the Trichodesmium or Crocosphaera watsonii isolate.</p>
http://lod.bco-dmo.org/id/dataset-parameter/35214.rdf
Name: s_to_v_ratio
Units: ratio (square:cubic micrometers)
Description: <p>Surface area (um^2) to volume (um^3) ratio.</p>
http://lod.bco-dmo.org/id/dataset-parameter/35215.rdf
Name: half_sat
Units: ppm pCO2
Description: <p>Half-saturation value, K1/2</p>
http://lod.bco-dmo.org/id/dataset-parameter/35216.rdf
Name: half_sat_sd
Units: ppm pCO2
Description: <p>Standard deviation of K1/2.</p>
http://lod.bco-dmo.org/id/dataset-parameter/35217.rdf
Name: vmax
Units: umol N mg Chla-1 h-1
Description: <p>Vmax</p>
http://lod.bco-dmo.org/id/dataset-parameter/35218.rdf
Name: vmax_sd
Units: umol N mg Chla-1 h-1
Description: <p>Standard deviation of Vmax.</p>
http://lod.bco-dmo.org/id/dataset-parameter/35219.rdf
Name: pCO2
Units: parts per million (ppm)
Description: <p>pCO2 level of the experiment.</p>
http://lod.bco-dmo.org/id/dataset-parameter/35220.rdf
Name: N2_fixation
Units: picomoles Nitrogen per nanogram chl-a per hour (pmol N ng Chla-1 h-1)
Description: <p>Nitrogen fixation rate normalized to culture chlorophyll-a levels (pmol N ng Chla-1 h-1).</p>
http://lod.bco-dmo.org/id/dataset-parameter/35221.rdf
Name: N2_fixation_sd
Units: picomoles Nitrogen per nanogram chl-a per hour (pmol N ng Chla-1 h-1)
Description: <p>Standard deviation of N2 fixation rate normalized to culture chlorophyll-a levels.</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
3015
https://datadocs.bco-dmo.org/file/GwwAk3GTLmjW7B/cyano_fixation.csv
cyano_fixation.csv
Primary data file for dataset ID 3966
download
https://osprey.bco-dmo.org/dataset/3966/data/download
download
onLine
dataset
<p>The seven isolates were grown across a pCO2 range in triplicate steady-state semi-continuous cultures at 28 degrees C on a 12 h dark:12 h light cycle using cool white fluorescent bulbs at 120 µmol photons m-2 s-1, in 0.2 µm-filtered, microwave-sterilized artificial seawater enriched with 20 µM phosphate and Aquil trace metals and vitamins, but with no fixed nitrogen (Hutchins et al. 2007, Fu et al. 2008). Cultures were diluted every 2-3 days based on their growth rates, and were sampled when acclimation to experimental conditions was verified by statistically invariant growth rates over 10-15 generations. For <em>Trichodesmium</em> KO4-20, H9-4, and 2174 and <em>Crocosphaera</em> WH0401, WH0003 and WH8501 experimental CO2 concentrations were 100ppm, 190ppm, 280ppm, 750ppm, 1500ppm, and 2000ppm. The CO2 response curve for <em>Trichodesmium</em> GBR was obtained from data from a previous experiment (Hutchins et al. 2007) at concentrations of 150ppm, 370ppm, 750ppm, 1250ppm and 1500 ppm. Gentle bubbling with certified commercial air/CO2 mixtures (Praxair) was used to obtain these seawater concentrations, which were verified by dissolved inorganic carbon (DIC) and pH measurements (Hutchins et al. 2007, Fu et al. 2008). Triplicate preserved 25 mL DIC samples (200 µL 5% HgCl2) were stored in borosilicate flasks at 4 degrees C for &lt;7 days until analysis. Total DIC was measured coulomb-metrically (model CM 140, UIC, Joliet, IL, USA) and pH was monitored daily using a microprocessor pH meter (NBS system) with three point buffer calibrations. Certified pCO2 values were verified using measured DIC and pH values with CO2 SYS software (<a href="http://cdiac.ornl.gov/ftp/co2sys/" target="_blank">http://cdiac.ornl.gov/ftp/co2sys/</a>). Since measured values never deviated &gt;1-2% from commercial certified values, pCO2 is reported as the latter value.</p>
<p>Rates of N2 fixation were measured at the same time of day for each culture (during the dark period for <em>Crocosphaera </em>and light period for <em>Trichodesmium</em>) with the acetylene reduction method using a 3:1 ratio to convert ethylene production to N2 fixation, and were normalized to culture chlorophyll a levels. Microscope counts were used to calculate cell-specific exponential growth rates (µ)( N<sub>T</sub>=N<sub>0</sub>e<sup>uT</sup>, where N is the initial cell density, N<sub>T</sub> is the cell density one day later, and T is one day) (Hutchins et al. 2007, Fu et al. 2008).</p>
<p>CO2 response curves for N2 fixation rates in each of the triplicate cultures for each isolate in each pCO2 treatment were fitted to Michaelis-Menten (1913) rectangular hyperbolic saturation equation curves (N2 fixation rate = Vmax * pCO2 / (K1/2 + pCO2)) using SigmaPlot software (SPSS), including determination of kinetic constants and curve correlation coefficients. Means and standard deviations of K1/2 (ppmv CO2) and Vmax (µmol N mg Chl a-1 h-1) values from the triplicate response curves are reported; significance of differences in K1/2 and Vmax values between isolates were tested using one-way ANOVA, followed by Fisher's Least Significant Difference to compare the mean of one group with the mean of another with SPSS statistics software (Hutchins et al. 2007, Fu et al. 2008).&nbsp; Multi-variate Principle Coordinate Analysis (PCoA) and Hierarchical Clustering were used to analyze the variance between all treatments and replicates in order to group them using their K1/2 and Vmax values as metrics of their responses to the CO2 treatments (Ramette 2007).</p>
<p><strong>References</strong><br />
Fu, F.-X., Mulholland, M.R., Garcia, N., Beck, A., Bernhardt, P.W., Warner, M.E., Sañudo-Wilhelmy, S.A. and Hutchins, D.A.&nbsp; 2008. Interactions between changing pCO2, N2 fixation, and Fe limitation in the marine unicellular cyanobacterium Crocosphaera. Limnology and Oceanography 53 (6): 2472- 2484. DOI: <a href="http://dx.doi.org/10.4319/lo.2008.53.6.2472" target="_blank">10.4319/lo.2008.53.6.2472</a></p>
<p>Hynes, A.M., Webb, E.A., Doney, S.C., and Waterbury, J.B. 2012. Comparison of cultured Trichodesmium (Cyanophyceae) with species characterized from the field. Journal of Phycology 48: 196-210. DOI:&nbsp;<a href="http://dx.doi.org/10.1111/j.1529-8817.2011.01096.x" target="_blank">10.1111/j.1529-8817.2011.01096.x</a></p>
<p>Hutchins, D. A., Fu, F.-X., Zhang, Y., Warner, M. E., Feng, Y. et al.&nbsp; 2007. CO2 control of Trichodesmium N2 fixation, photosynthesis, growth rates, and elemental ratios:&nbsp; Implications for past, present, and future ocean biogeochemistry. Limnology and Oceanography 52: 1293-1304. DOI: <a href="http://dx.doi.org/10.4319/lo.2007.52.4.1293" target="_blank">10.4319/lo.2007.52.4.1293</a></p>
<p>Michaelis, L., and Menten, M. M. 1913. Die Kinetik der Invertinwirkung. Biochem. Z. 49: 333–369.</p>
<p>Ramette, Alban. 2007. Multivariate analyses in microbial ecology. FEMS Microbiol. Ecol. 62: 142–160. DOI:&nbsp;<a href="http://dx.doi.org/10.1111/j.1574-6941.2007.00375.x" target="_blank">10.1111/j.1574-6941.2007.00375.x</a></p>
<p>Webb, E. A., Ehrenreich, I. M., Brown, S. L., Valois, F. W., and Waterbury, J. B. 2009. Phenotypic and genotypic characterization of multiple strains of the diazotrophic cyanobacterium, <em>Crocosphaera watsonii</em>, isolated from the open ocean. Environmental Microbiology 11:338-348. DOI:&nbsp;<a href="http://dx.doi.org/10.1111/j.1462-2920.2008.01771.x" target="_blank">10.1111/j.1462-2920.2008.01771.x</a></p>
Specified by the Principal Investigator(s)
<p>BCO-DMO re-arranged data formatted as separate tables into one dataset. Parameter names were changed to conform with BCO-DMO conventions.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Benchtop pH Meter
Benchtop pH Meter
PI Supplied Instrument Name: Benchtop pH Meter PI Supplied Instrument Description:pH was monitored daily using a microprocessor pH meter (NBS system). Instrument Name: Benchtop pH Meter Instrument Short Name:Benchtop pH Meter Instrument Description: An instrument consisting of an electronic voltmeter and pH-responsive electrode that gives a direct conversion of voltage differences to differences of pH at the measurement temperature. (McGraw-Hill Dictionary of Scientific and Technical Terms)
This instrument does not map to the NERC instrument vocabulary term for 'pH Sensor' which measures values in the water column. Benchtop models are typically employed for stationary lab applications.
CO2 Coulometer
CO2 Coulometer
PI Supplied Instrument Name: CO2 Coulometer PI Supplied Instrument Description:Total DIC was measureed coulomb-metrically (CM140, UIC, Joliet, IL). CM140 Instrument Brochure. Instrument Name: CO2 Coulometer Instrument Short Name:CO2 coulometer Instrument Description: A CO2 coulometer semi-automatically controls the sample handling and extraction of CO2 from seawater samples. Samples are acidified and the CO2 gas is bubbled into a titration cell where CO2 is converted to hydroxyethylcarbonic acid which is then automatically titrated with a coulometrically-generated base to a colorimetric endpoint. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB12
Deployment: lab_Hutchins_07-12_diazotrophs
lab_Hutchins_07-12_diazotrophs
USC
laboratory
lab_Hutchins_07-12_diazotrophs
David A. Hutchins
University of Southern California
USC
laboratory