<div><p>Approximate sampling positions (latitude and longitude) were determined from navigational maps.</p></div>
samples around Long Island, NY for Labyrinthulomycete PCR and sequences
<div><p>Accession numbers with links to GenBank sequences of labyrinthulomycete 18S rRNA genes from sediment and water column samples collected around Long Island, NY.</p>
<p>Collado Mercado et al, 2010 ABSTRACT: Labyrinthulomycetes (Labyrinthulea) are ubiquitous marine osmoheterotrophic protists that appear to be important in decomposition of both allochthonous and autochthonous organic matter. We used a cultivation-independent method based on the labyrinthulomycete-specific primer LABY-Y to PCR amplify, clone, and sequence 68 nearly full-length 18S rDNA amplicons from 4 sediment and 3 seawater samples collected in estuarine habitats around Long Island, New York, USA. Phylogenetic analyses revealed that all 68 amplicons belonged to the Labyrinthulea. Only 15 of the 68 amplicons belonged to the thraustochytrid phylogenetic group (Thraustochytriidae). None of these 15 were similar to cultivated strains, and 11 formed a novel group. The remaining 53 amplicons belonged either to the labyrinthulid phylogenetic group (Labyrinthulidae) or to other families of Labyrinthulea that have not yet been described. Of these amplicons, 37 were closely related to previously cultivated Aplanochytrium spp. and Oblongichytrium spp. Members of these 2 genera were also cultivated from 1 of the sediment samples. The 16 other amplicons were not closely related to cultivated strains, and 15 belonged to 5 groups of apparently novel labyrinthulomycetes. Most of the novel groups of amplicons also contained environmental sequences from surveys of protist diversity using universal 18S rDNA primers. Because the primer LABY-Y is biased against several groups of labyrinthulomycetes, particularly among the thraustochytrids, these results may underestimate the undiscovered diversity of labyrinthulomycetes.</p></div>
Labyrinthulomycete genetic sequences
<div><p>See:<br />
Collado Mercado, E., J. C. Radway, and J. L. Collier. 2010. Novel uncultivated labyrinthulomycetes revealed by 18S rDNA sequences from seawater and sediment samples. Aquatic Microbial Ecology 58: 215-228. <a href="http://www.int-res.com/articles/ame2010/58/a058p215.pdf" target="_blank">[pdf]</a></p>
<p>Sequence accession numbers deposited in GenBank are indicated in the data file via hyperlinks.</p></div>
3974
Labyrinthulomycete genetic sequences
2013-06-25T15:03:50-04:00
2013-06-25T15:03:50-04:00
2023-07-07T16:10:26-04:00
urn:bcodmo:dataset:3974
Accession numbers with links to GenBank sequences of labyrinthulomycete 18S rRNA genes from sediment and water column samples collected around Long Island, NY from 2005-2008 (LabyLI project)
false
Collier, J. L. (2013) Accession numbers with links to GenBank sequences of labyrinthulomycete 18S rRNA genes from sediment and water column samples collected around Long Island, NY from 2005-2008 (LabyLI project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 06/25/2013) Version Date 2013-06-25 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/3974 [access date]
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2013-06-25
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