Dataset:Inorganic nutrients (PO4, SiOH4, NO3, NO2, NH4) from R/V Knorr cruise KN207-01 from the southern tip of Nova Scotia to Bermuda in 2012 (SargassoSeaLipids project)
Project(s):Biogeochemical Impact and Fate of Non-phosphorus Membrane Lipids in the Sargasso Sea (SargassoSeaLipids)
Description:Inorganic nutrients (PO4, SiOH4, NO3, NO2, NH4) from KN207-01 cruise.

PO4, SiOH4, NO3, NO2, and NH4 from the KN207-01 cruise.

Acquisition Description:

Seawater was collected with Niskin bottles. Samples were pre-filtered though a 0.2 micrometer filter into and acid-washed HDPE bottle and frozen at -20 degrees C. Samples were shipped frozen to the University of Washington Marine Chemistry Laboratory. Samples were analyzed in January 2013 on a Technicon AAII Autoanalyzer.

Analytical methods (from http://www.ocean.washington.edu/story/Marine+Chemistry+Laboratory):

Analysis Method References EPA/SM# NELAC Code 2011 Detection_Limits
PO4 UNESCO(1994) EPA 365.5_1.4_1997 WM920270 0.03uM
SiOH4 UNESCO(1994) EPA 366 WM920240 0.76uM 
NO3 UNESCO(1994) EPA 353.4_2_199 10068209 0.08uM
NO2 UNESCO(1994) EPA 353.4_2_1997 10068209 0.01uM
NH4 UNESCO(1994) EPA 349 WM920220 0.07uM
Total N Valderrama(1981) SM 4500-P J WM920270 1.08uM
Total P Valderrama(1981) SM 4500-P J WM920270 0.04uM
Processing Description:

BCO-DMO added date and lat/lon from the CTD data based on cast number.

Project Information

Biogeochemical Impact and Fate of Non-phosphorus Membrane Lipids in the Sargasso Sea

Intact polar diacyglycerols (IP-DAGs) are the fatty-acid bearing lipid molecules that compose bacterial and eukaryotic cell membranes. As such, they are one of the most abundant classes of lipid molecules in plankton, and play a major role in the marine carbon cycle. However, until very recently, the molecular diversity of IP-DAGs was poorly understood; the structural identity and characteristics of IP-DAGs were inferred almost exclusively from their constituent fatty acids. These non-phosphorus containing IP-DAGs were largely unknown to chemical oceanography. In contrast, phospholipids, which have been the focus of considerable research, compose a disproportionally small fraction of total IP-DAGs. But we still lack even a cursory understanding of biochemical functions and geochemical fates of non-phosphorus IP-DAGs. Given that these molecules are among the most abundant lipid molecules on the planet, this represents a profound and unexpected gap in our understanding the marine carbon and phosphorus cycles. In this project, researchers at the Woods Hole Oceanographic Institution will launch a pioneering study of these poorly understood compounds. Their approach will be guided by four questions: (1) How do non-phosphorus lipids contribute to variations in the C:N:P of particulate organic matter in the Sargasso Sea? (2) What are the relative degradation rates of phospholipids and non-phosphorus lipids in surface waters? (3) Which groups of microbes utilize the carbon and phosphorus from different IP-DAGs? (4) What are the relative contributions of different IP-DAGs to particulate organic matter export to the deep-sea? These questions will be answered by using sophisticated HPLC/MS analyses and novel isotope tracing approaches in conjunction with long-standing methods for measuring the C:N:P of plankton and determining the degradation rates of organic molecules. The research team will establish whether these newly-recognized sulfolipids and betaine lipids molecules are a quantitatively important biochemical option for phytoplankton to affect flexible C:N:P stoichiometry in the face of nutrient stress. They will also elucidate the degradation rate, microbial fate, and export potential of the carbon and phosphorus from IP-DAGs. This will shed new light on the broader roles of these molecules in the cycling of these elements by the planktonic community. This project contains components that are specifically designed to meet the NSF criteria for "advancing discovery and understanding while promoting teaching, training and learning." The project will support the training of a graduate student and postdoctoral fellow. In addition, the research team will work with the non-profit Zephyr Foundation in Woods Hole to design educational 'units' based on the team's research that will be tailored to student in grades 6 - 12. The Foundation will present these units as part of their hands-on marine science field trip series that is delivered to over 200 students and their teachers per year.



Deployment Information

Deployment description for R/V Knorr KN207-01

Projected Science Plan: The plan is to conduct two, 5-day quasi-lagrangian time-series stations at 65W, one north of the Gulf Stream and one south of the Gulf Stream.  The daily cruise track will be centered around following free-floating sediment net traps arrays.  The traps will be retrieved and re-deployed on 24 hour intervals (generally beginning at day break).  CTD casts, primarily in the upper 250 meters, will be done in the afternoons, with McLane pumps deployed overnight. This cruise is funded by NSF OCE-1031143. More information about this cruise is available from the vessel operator (WHOI cruise synopsis). Cruise information and original data are available from the NSF R2R data catalog.



Instrument Information

Instrument Nutrient Autoanalyzer
Description Samples analyzed by Kathy Krogslund at University of Washington in January 2013 on Technicon AAII autoanalyzer.
Generic Instrument Name Nutrient Autoanalyzer
Generic Instrument Description Nutrient Autoanalyzer is a generic term used when specific type, make and model were not specified. In general, a Nutrient Autoanalyzer is an automated flow-thru system for doing nutrient analysis (nitrate, ammonium, orthophosphate, and silicate) on seawater samples.