Table of Contents

• Coverage
• Dataset Description
  • Methods & Sampling
  • Data Processing Description
• Data Files
• Parameters
• Instruments
• Deployments
• Project Information
• Program Information
• Funding

PO4, SiOH4, NO3, NO2, and NH4 from the KN207-01 cruise.

Seawater was collected with Niskin bottles. Samples were pre-filtered though a 0.2 micrometer filter into and acid-washed HDPE bottle and frozen at -20 degrees C. Samples were shipped frozen to the University of Washington Marine Chemistry Laboratory. Samples were analyzed in January 2013 on a Technicon AAII Autoanalyzer.

Analytical methods (from http://www.ocean.washington.edu/story/Marine+Chemistry+Laboratory):

BCO-DMO added date and lat/lon from the CTD data based on cast number.

Intact polar diacyglycerols (IP-DAGs) are the fatty-acid bearing lipid molecules that compose bacterial and eukaryotic cell membranes. As such, they are one of the most abundant classes of lipid molecules in plankton, and play a major role in the marine carbon cycle. However, until very recently, the molecular diversity of IP-DAGs was poorly understood; the structural identity and characteristics of IP-DAGs were inferred almost exclusively from their constituent fatty acids. These non-phosphorus containing IP-DAGs were largely unknown to chemical oceanography. In contrast, phospholipids, which have been the focus of considerable research, compose a disproportionally small fraction of total IP-DAGs. But we still lack even a cursory understanding of biochemical functions and geochemical fates of non-phosphorus IP-DAGs. Given that these molecules are among the most abundant lipid molecules on the planet, this represents a profound and unexpected gap in our understanding the marine carbon and phosphorus cycles.

In this project, researchers at the Woods Hole Oceanographic Institution will launch a pioneering study of these poorly understood compounds. Their approach will be guided by four questions: (1) How do non-phosphorus lipids contribute to variations in the C:N:P of particulate organic matter in the Sargasso Sea? (2) What are the relative degradation rates of phospholipids and non-phosphorus lipids in surface waters? (3) Which groups of microbes utilize the carbon and phosphorus from different IP-DAGs? (4) What are the relative contributions of different IP-DAGs to particulate organic matter export to the deep-sea?

These questions will be answered by using sophisticated HPLC/MS analyses and novel isotope tracing approaches in conjunction with long-standing methods for measuring the C:N:P of plankton and determining the degradation rates of organic molecules. The research team will establish whether these newly-recognized sulfolipids and betaine lipids molecules are a quantitatively important biochemical option for phytoplankton to affect flexible C:N:P stoichiometry in the face of nutrient stress. They will also elucidate the degradation rate, microbial fate, and export potential of the carbon and phosphorus from IP-DAGs. This will shed new light on the broader roles of these molecules in the cycling of these elements by the planktonic community.

This project contains components that are specifically designed to meet the NSF criteria for "advancing discovery and understanding while promoting teaching, training and learning." The project will support the training of a graduate student and postdoctoral fellow. In addition, the research team will work with the non-profit Zephyr Foundation in Woods Hole to design educational 'units' based on the team's research that will be tailored to student in grades 6 - 12. The Foundation will present these units as part of their hands-on marine science field trip series that is delivered to over 200 students and their teachers per year.

The Ocean Carbon and Biogeochemistry (OCB) program focuses on the ocean's role as a component of the global Earth system, bringing together research in geochemistry, ocean physics, and ecology that inform on and advance our understanding of ocean biogeochemistry. The overall program goals are to promote, plan, and coordinate collaborative, multidisciplinary research opportunities within the U.S. research community and with international partners. Important OCB-related activities currently include: the Ocean Carbon and Climate Change (OCCC) and the North American Carbon Program (NACP); U.S. contributions to IMBER, SOLAS, CARBOOCEAN; and numerous U.S. single-investigator and medium-size research projects funded by U.S. federal agencies including NASA, NOAA, and NSF.

The scientific mission of OCB is to study the evolving role of the ocean in the global carbon cycle, in the face of environmental variability and change through studies of marine biogeochemical cycles and associated ecosystems.

The overarching OCB science themes include improved understanding and prediction of: 1) oceanic uptake and release of atmospheric CO2 and other greenhouse gases and 2) environmental sensitivities of biogeochemical cycles, marine ecosystems, and interactions between the two.

The OCB Research Priorities (updated January 2012) include: ocean acidification; terrestrial/coastal carbon fluxes and exchanges; climate sensitivities of and change in ecosystem structure and associated impacts on biogeochemical cycles; mesopelagic ecological and biogeochemical interactions; benthic-pelagic feedbacks on biogeochemical cycles; ocean carbon uptake and storage; and expanding low-oxygen conditions in the coastal and open oceans.