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        <gco:CharacterString>WS1005 cruise track (1-min fixes; from R2R). Dataset Description: &amp;lt;p&amp;gt;1-minute resolution navigation from the WS1005 cruise aboard the R/V F.G. Walton Smith from 18 March to 24 March 2010.&amp;lt;/p&amp;gt; Methods and Sampling: &amp;lt;p&amp;gt;Original navigation and other data are available from the NSF R2R data catalog: &amp;lt;a href=&amp;quot;http://www.rvdata.us/catalog/WS1005&amp;quot; target=&amp;quot;_blank&amp;quot;&amp;gt;http://www.rvdata.us/catalog/WS1005&amp;lt;/a&amp;gt;&amp;lt;/p&amp;gt;</gco:CharacterString>
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&lt;p&gt;Microbial  mats are conspicuous components of many benthic marine and aquatic  settings.  A subset of these microbial mats binds sediments to form  potentially fossilizable structures, often called stromatolites or  microbialites. While much is known about microbialite autotrophs, little  is known about their heterotrophic eukaryotes. The lack of  understanding is surprising given that stromatolites have an extensive  geologic record spanning most of Earth's history. Stromatolites are  layered sedimentary structures formed by a combination of microbial  activities, abiotic carbonate precipitation, and sedimentary processes.  Details of stromatolite formation and preservation are poorly  understood, and a drastic decline in stromatolite occurrence and  diversity in the late Precambrian has long been a conundrum. A popular  hypothesis to explain this decline at ~1 billion years ago is that  eukaryotic organisms evolved to become predators on stromatolites. To  date, the most commonly proposed predatory culprit is an unidentified  metazoan, although evidence of such an organism is lacking from the  fossil record. Protists, most of which are not expected to leave an  obvious fossil record, are additional possible stromatolitic predators,  but they have been largely ignored in this context. The hypotheses of  this project are: (1) Heterotrophic protist activity caused the textural  change from stromatolites (layered sediment fabric) to thrombolites  (clotted sediment fabric) and (2) Heterotrophic protists caused the  decimation of Neoproterozoic stromatolites. Since it is impossible to  recreate the Neoproterozoic, studies of modern analogs serve to  indirectly test these hypotheses. The overall goal of this project is to  describe the eukaryotic communities associated with modern  stromatolites and thrombolites from the Bahamas and Australia, compare  the communities from the two sites, and to relate the communities to  stromatolitic / thrombolitic sediment fabric and biomarker signatures. &lt;/p&gt;
&lt;p&gt;The  overall goal will be achieved by addressing the following specific  aims: (1) Identify, via morphologic and molecular approaches, the  eukaryotic community of modern stromatolites and thrombolites; (2)  Analyze modern and fossil stromatolites and thrombolites for their  eukaryotic lipid biomarkers using solvent extraction, chromatographic  and mass spectrometric methods; (3) Using the Fluorescently Labeled  Embedded Core (FLEC) method, document the sub-millimeter distributions  of the heterotrophic eukaryotic community inhabiting modern  stromatolites and thrombolites in conjunction with fine-scale sediment  fabric; (4) Using solvent extraction, chromatographic and mass  spectrometric methods, analyze cultures of allogromiid foraminifers to  survey for lipid biomarkers unique to them; (5)  After incubation of  modern stromatolites with heterotrophic protists, use FLEC methodology  to determine how their activity affects sediment fabric and conduct  preliminary comparisons of these modern fabrics to those of stromatolite  fossils. &lt;/p&gt;
&lt;p&gt;Intellectual Merit: The oldest fossil  stromatolites are &amp;gt;3.4 billion years old and are the most visible  manifestations of pervasive microbial life on the early Earth. The  changes in stromatolite abundance and morphology document complex  interplays between biological and geological processes. This project  addresses multiple aspects of stromatolite genesis and pre-fossilization  alteration but at its core, focuses on one of the greatest geological  enigmas: the possible connection between stromatolite decline and the  rise of complex life.&lt;/p&gt;</gco:CharacterString>
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