<div><p>In July 2011, eighteen colonies of the tropical coral <em>S. siderea</em> were collected at 3 to 5 m depth on the Mesoamerican Barrier Reef System in southern Belize. Colonies were collected from nearshore, backreef, and forereef reef zones. <em>Siderastrea siderea</em> corals were transported to the University of North Carolina at Chapel Hill and each coral colony was sectioned into 18 comparatively sized specimens (approximately 3 cm x 2 cm x 1 cm) using a rock saw and glued with cyanoacrylate on to plastic microscope slides. The corals were allowed to recover for approximately 30 days under laboratory conditions in two 500 L recirculating artificial seawater systems until the start of the 15-day acclimation period, in which the corals were incrementally exposed to the experimental treatment conditions.</p>
<p>In the laboratory experiments, <em>Siderastrea siderea</em> coral specimens from each of the 18 colonies were reared for 95 days (5 August − 8 November 2011) in each of twelve 38 L glass aquaria (18 specimens per tank; 216 specimens in total) filled with artificial seawater formulated at a salinity of 35 with <em>Instant Ocean Sea Salt</em> and deionized water. Four pCO2 partial pressures [324, 477, 604, and 2553) ppm)], established by mixing pure CO2 with compressed air using <em>Aalborg</em> mass flow controllers, were bubbled with microporous ceramic airstones into the triplicate glass aquaria (12 tanks total). Coral specimens from each of the 18 colonies were reared in each of the 12 replicate tanks. The pCO2 experiments were maintained at an average temperature of 28 ºC.</p>
<p>Experimental growth conditions for the seawater temperature experiment were similar to those for the CO2-induced ocean acidification experiment described above. <em>Siderastrea siderea</em> coral specimens from each of the 18 colonies were reared for 95 days (5 August − 8 November 2011) in each of nine 38 L glass aquaria (18 specimens per tank; 162 specimens in total) filled with artificial seawater formulated at a salinity of 35 with <em>Instant Ocean Sea Salt</em> and deionized water. Three experimental seawater temperatures [25, 28, and 32 ºC] were maintained in triplicate (9 tanks total) for this experiment. Coral specimens from each of the 18 colonies were reared in each of the 9 replicate tanks. Compressed air with an average pCO2 of 488 ppm was bubbled with microporous ceramic airstones into the triplicate glass aquaria.</p>
<p>Coral calcification rates were estimated using a buoyant weight technique. <em>Siderastrea siderea</em> specimens were weighed at the beginning of the experiment and a final measurement taken at approximately 95 days. Each coral specimen was suspended by aluminum wire at 10-cm depth from a <em>Cole-Parmer</em> bottom-loading scale (precision ± 0.001; accuracy ± 0.002) in an aquarium filled with artificial seawater maintained at a temperature of 25 ºC and salinity of 33. A standardized plastic-coated zinc mass was intermittently weighed to ensure consistency of the buoyant weight method throughout the duration of the experiment.</p></div>
MBRS coral buoyant weights for temperature experiments
<div><p>MBRS coral buoyant weights for temperature experiments</p></div>
BW MBRSCorals - Temperature Experiments
<div><p>These experiments utilize a combined repeated measures/split-plot design. Temperature and pCO2 represent whole-plot treatments while the reef zone of an individual coral colony represents a split-plot treatment. Hierarchical mixed-effects models were employed to account for this experimental design. Time measurements were treated as nested in corals, and corals were treated as nested in aquaria. Each coral's measured buoyant weight (mg) was divided by its initial surface area (cm2) to yield a normalized weight (mg cm-2). In both the pCO2 and temperature experiments normalized buoyant weight (mg cm-2) was regressed against continuous time and treatment to assess the overall effect of treatment on <em>S. siderea</em> calcification rates for the 95-day duration of the experiments. Of interest here was the coefficient of time in the regression model and the extent to which it varied by treatment. Normalized calcification rate (mg cm-2 d-1) was then obtained by extracting the regression coefficient of the continuous time variable in the model. Difference-adjusted confidence intervals were used to provide a simple graphical display of the differences among pCO2 and temperature treatment groups. All mixed models were estimated with the nlme package of R 2.15.2.</p>
<p><strong>BCO-DMO Processing Notes</strong><br />
- Generated from original files "Temp_Expt_BW_MBRSCorals.csv" contributed by Karl Castillo</p></div>
4000
BW MBRSCorals - Temperature Experiments
2013-07-19T13:36:07-04:00
2013-07-19T13:36:07-04:00
2023-07-07T16:10:26-04:00
urn:bcodmo:dataset:4000
Mesoamerican Barrier Reef System coral buoyant weights for temperature experiments from Sapodilla Caye, Belize starting 2010 (OA - Ocean Acidification and Warming Impact on Calcification project)
false
Ries, J. B. (2013) Mesoamerican Barrier Reef System coral buoyant weights for temperature experiments from Sapodilla Caye, Belize starting 2010 (OA - Ocean Acidification and Warming Impact on Calcification project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 22 July 2013) Version Date 2013-07-22 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/4000 [access date]
false
22 July 2013
false
2013-07-22
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