http://lod.bco-dmo.org/id/dataset/4000
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2013-07-19
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Mesoamerican Barrier Reef System coral buoyant weights for temperature experiments from Sapodilla Caye, Belize starting 2010 (OA - Ocean Acidification and Warming Impact on Calcification project)
2013-07-22
publication
2013-07-22
revision
BCO-DMO Linked Data URI
2013-07-22
creation
http://lod.bco-dmo.org/id/dataset/4000
Justin B. Ries
University of North Carolina at Chapel Hill
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Ries, J. B. (2013) Mesoamerican Barrier Reef System coral buoyant weights for temperature experiments from Sapodilla Caye, Belize starting 2010 (OA - Ocean Acidification and Warming Impact on Calcification project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 22 July 2013) Version Date 2013-07-22 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/4000 [access date]
MBRS coral buoyant weights for temperature experiments Dataset Description: <p>MBRS coral buoyant weights for temperature experiments</p> Methods and Sampling: <p>In July 2011, eighteen colonies of the tropical coral <em>S. siderea</em> were collected at 3 to 5 m depth on the Mesoamerican Barrier Reef System in southern Belize. Colonies were collected from nearshore, backreef, and forereef reef zones. <em>Siderastrea siderea</em> corals were transported to the University of North Carolina at Chapel Hill and each coral colony was sectioned into 18 comparatively sized specimens (approximately 3 cm x 2 cm x 1 cm) using a rock saw and glued with cyanoacrylate on to plastic microscope slides.&nbsp; The corals were allowed to recover for approximately 30 days under laboratory conditions in two 500 L recirculating artificial seawater systems until the start of the 15-day acclimation period, in which the corals were incrementally exposed to the experimental treatment conditions.<br />
<br />
In the laboratory experiments, <em>Siderastrea siderea</em> coral specimens from each of the 18 colonies were reared for 95 days (5 August − 8 November 2011) in each of twelve 38 L glass aquaria (18 specimens per tank; 216 specimens in total) filled with artificial seawater formulated at a salinity of 35 with <em>Instant Ocean Sea Salt</em> and deionized water. Four pCO2 partial pressures [324, 477, 604, and 2553) ppm)], established by mixing pure CO2 with compressed air using <em>Aalborg</em> mass flow controllers, were bubbled with microporous ceramic airstones into the triplicate glass aquaria (12 tanks total).&nbsp; Coral specimens from each of the 18 colonies were reared in each of the 12 replicate tanks.&nbsp; The pCO2 experiments were maintained at an average temperature of 28 ºC.<br />
<br />
Experimental growth conditions for the seawater temperature experiment were similar to those for the CO2-induced ocean acidification experiment described above.&nbsp; <em>Siderastrea siderea</em> coral specimens from each of the 18 colonies were reared for 95 days (5 August − 8 November 2011) in each of nine 38 L glass aquaria (18 specimens per tank; 162 specimens in total) filled with artificial seawater formulated at a salinity of 35 with <em>Instant Ocean Sea Salt</em> and deionized water. Three experimental seawater temperatures [25, 28, and 32 ºC] were maintained in triplicate (9 tanks total) for this experiment. Coral specimens from each of the 18 colonies were reared in each of the 9 replicate tanks.&nbsp; Compressed air with an average pCO2 of 488 ppm was bubbled with microporous ceramic airstones into the triplicate glass aquaria.<br />
<br />
Coral calcification rates were estimated using a buoyant weight technique.&nbsp; <em>Siderastrea siderea</em> specimens were weighed at the beginning of the experiment and a final measurement taken at approximately 95 days. Each coral specimen was suspended by aluminum wire at 10-cm depth from a <em>Cole-Parmer</em> bottom-loading scale (precision ± 0.001; accuracy ± 0.002) in an aquarium filled with artificial seawater maintained at a temperature of 25 ºC and salinity of 33. A standardized plastic-coated zinc mass was intermittently weighed to ensure consistency of the buoyant weight method throughout the duration of the experiment.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1031995 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1031995
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1357665 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1357665
onGoing
Justin B. Ries
University of North Carolina at Chapel Hill
781-581-7370
Northeastern University Marine Science Center 430 Nahant Rd.
Nahant
MA
01908
USA
j.ries@northeastern.edu
pointOfContact
asNeeded
Dataset Version: 22 July 2013
Unknown
No
Reefzone
Colony
Coral_ID
Tank_No
pCO2
Temperature
Buoyant_Weight_T0
Buoyant_Weight_T90
Microporous Ceramic Airstones
38 L glass aquaria
Aalborg Mass Flow Controller
Cole-Parmer Bottom-loading Scale
theme
None, User defined
number
Site_ID
sample identification
tank
Partial pressure of CO2
water temperature at measurement depth
weight
featureType
BCO-DMO Standard Parameters
Airstone
Aquarium
Mass Flow Controller
scale or balance
instrument
BCO-DMO Standard Instruments
lab_Ries_Sapodilla_Caye
lab_Ries_UNC_Chapel_Hill
service
Deployment Activity
Sapodilla Caye, Belize (16.2 N 88.5 W)
University of North Carolina - Chapel Hill
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Ocean Carbon and Biogeochemistry
http://us-ocb.org/
Ocean Carbon and Biogeochemistry
The Ocean Carbon and Biogeochemistry (OCB) program focuses on the ocean's role as a component of the global Earth system, bringing together research in geochemistry, ocean physics, and ecology that inform on and advance our understanding of ocean biogeochemistry. The overall program goals are to promote, plan, and coordinate collaborative, multidisciplinary research opportunities within the U.S. research community and with international partners. Important OCB-related activities currently include: the Ocean Carbon and Climate Change (OCCC) and the North American Carbon Program (NACP); U.S. contributions to IMBER, SOLAS, CARBOOCEAN; and numerous U.S. single-investigator and medium-size research projects funded by U.S. federal agencies including NASA, NOAA, and NSF.
The scientific mission of OCB is to study the evolving role of the ocean in the global carbon cycle, in the face of environmental variability and change through studies of marine biogeochemical cycles and associated ecosystems.
The overarching OCB science themes include improved understanding and prediction of: 1) oceanic uptake and release of atmospheric CO2 and other greenhouse gases and 2) environmental sensitivities of biogeochemical cycles, marine ecosystems, and interactions between the two.
The OCB Research Priorities (updated January 2012) include: ocean acidification; terrestrial/coastal carbon fluxes and exchanges; climate sensitivities of and change in ecosystem structure and associated impacts on biogeochemical cycles; mesopelagic ecological and biogeochemical interactions; benthic-pelagic feedbacks on biogeochemical cycles; ocean carbon uptake and storage; and expanding low-oxygen conditions in the coastal and open oceans.
OCB
largerWorkCitation
program
Investigation of the Effects of CaCO3 Saturation State and Temperature on the Calcification Rate and Skeletal Properties of Benthic Marine Calcifiers
http://www.unc.edu/~jries/index.html
Investigation of the Effects of CaCO3 Saturation State and Temperature on the Calcification Rate and Skeletal Properties of Benthic Marine Calcifiers
<p><em>Description from NSF award abstract:</em><br />
Anthropogenic elevation of atmospheric pCO2 is increasing the acidity of the oceans, thereby reducing the saturation state of seawater with respect to calcium carbonate (CaCO3). Of mounting concern is the potential impact of these changes on the ability of calcifying organisms to form their shells and skeletons. Recent studies, including pilot work conducted by investigator Ries and his colleagues on a suite of benthic marine calcifiers spanning broad taxonomic, mineralogical, and ecological ranges, have revealed that marine organisms exhibit a wide range of calcification responses to CO2-induced ocean acidification, including positive, negative, parabolic, threshold, and neutral responses. Marine calcifiers build their shells and skeletons from various forms (polymorphs) of CaCO3, most commonly aragonite, high-Mg calcite, and low-Mg calcite. These polymorphs differ greatly in their solubility in seawater and, therefore, in their potential response to CO2-induced ocean acidification. X-ray diffraction analysis of shells secreted by the organisms investigated in the pilot study reveals that the proportion of calcite (the less soluble form of CaCO3) to aragonite (the more soluble form) within their shells increases under elevated pCO2, while the Mg:Ca ratio of their calcite declines. These observations suggested that some marine calcifiers may partially adapt to a declining CaCO3 saturation state by accreting a greater proportion of the less-soluble form of CaCO3 (low-Mg calcite) at the expense of the more soluble forms (aragonite, high-Mg calcite). However, it is likely that such mineralogical and compositional changes in the shells and skeletons of marine organisms would alter their structural and biomechanical properties.</p>
<p>The project seeks to build upon the results of a pilot study by rearing a suite of benthic marine calcifiers under past (280 ppm), present (385 ppm), and predicted future (540, 840 ppm) pCO2 and under three distinct temperatures to investigate changes in: (1) their rates of calcification and linear extension; (2) the relative abundance and micron-scale distribution of the various CaCO3 polymorphs within their shells/skeletons; (3) the ultrastructure and crystal morphology of their shells/skeletons; and (4) their biomechanical properties. The research also builds upon the pilot experiments by utilizing a more thoroughly replicated study design, by more precisely constraining the chemical parameters of the experimental seawater treatments, by investigating calcification responses under 3 different temperature regimes, and by employing a "pre-industrial" pCO2 level (280 ppm). The results of the proposed research should advance our understanding of how benthic marine calcifiers shall respond to future CO2-induced changes in seawater temperature and CaCO3 saturation state. By investigating the response of organisms over the range of atmospheric pCO2 that has occurred since late Paleozoic time, this research should inform our understanding of the putative links between atmospheric pCO2, mass extinction events, and secular variation in the polymorph mineralogy of marine calcifiers throughout geologic time. Finally, comparison of the observed biological responses to variable pCO2-T scenarios with that already established for abiogenic carbonates will advance our understanding of the very mechanisms by which marine calcifiers build their shells and skeletons.</p>
<p>Results of this research project will inform the decisions of policy makers and legislators working to mitigate the impacts of CO2-induced warming and ocean acidification by establishing pCO2-T tolerances for a range of marine calcifiers.</p>
<p><strong>Note (02 Oct 2014):</strong> Funding for this project has transferred from award OCE-1031995 to OCE-1357665, coincident with Principal Investigator's affiliation change from University of North Carolina at Chapel Hill to Northeastern University.</p>
OA - Ocean Acidification and Warming Impact on Calcification
largerWorkCitation
project
eng; USA
oceans
Sapodilla Caye, Belize (16.2 N 88.5 W); University of North Carolina - Chapel Hill
2013-07-22
Chapel Hill, North Carolina (lab) and Mesoamerican Barrier Reef System - Sapodilla Caye, Belize (16.2 N 88.5 W)
0
BCO-DMO catalogue of parameters from Mesoamerican Barrier Reef System coral buoyant weights for temperature experiments from Sapodilla Caye, Belize starting 2010 (OA - Ocean Acidification and Warming Impact on Calcification project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/35779.rdf
Name: No
Units: Integer
Description: <p>Number</p>
http://lod.bco-dmo.org/id/dataset-parameter/35780.rdf
Name: Reefzone
Units: text
Description: <p>Reefzone collection area<br />NS=Neashore<br />BR= Backreef<br />FR=Forereef</p>
http://lod.bco-dmo.org/id/dataset-parameter/35781.rdf
Name: Colony
Units: text
Description: <p> Individual <i>Siderastrea siderea</i> colony</p>
http://lod.bco-dmo.org/id/dataset-parameter/35782.rdf
Name: Coral_ID
Units: text
Description: <p>Individual coral piece from a selected colony<br />The letter represents the colony and the number indicate the number of that piece from that colony</p>
http://lod.bco-dmo.org/id/dataset-parameter/35783.rdf
Name: Tank_No
Units: integer
Description: <p>Replicate tanks (three per treatment)</p>
http://lod.bco-dmo.org/id/dataset-parameter/35784.rdf
Name: pCO2
Units: micro atmospheres
Description: <p>Partial pressure of mixed CO2 gases used to bubble seawater for the ocean acidification experiments</p>
http://lod.bco-dmo.org/id/dataset-parameter/35785.rdf
Name: Temperature
Units: degrees celsius
Description: <p>Temperature treatment</p>
http://lod.bco-dmo.org/id/dataset-parameter/35786.rdf
Name: Buoyant_Weight_T0
Units: grams
Description: <p>Initial buoyant weight</p>
http://lod.bco-dmo.org/id/dataset-parameter/35787.rdf
Name: Buoyant_Weight_T90
Units: grams
Description: <p>Final buoyant weight</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
5335
https://datadocs.bco-dmo.org/file/nGGlAvOTlPxx0q/BW_MBRSCorals_Temp_Expt.csv
BW_MBRSCorals_Temp_Expt.csv
Primary data file for dataset ID 4000
download
https://osprey.bco-dmo.org/dataset/4000/data/download
download
onLine
dataset
<p>In July 2011, eighteen colonies of the tropical coral <em>S. siderea</em> were collected at 3 to 5 m depth on the Mesoamerican Barrier Reef System in southern Belize. Colonies were collected from nearshore, backreef, and forereef reef zones. <em>Siderastrea siderea</em> corals were transported to the University of North Carolina at Chapel Hill and each coral colony was sectioned into 18 comparatively sized specimens (approximately 3 cm x 2 cm x 1 cm) using a rock saw and glued with cyanoacrylate on to plastic microscope slides.&nbsp; The corals were allowed to recover for approximately 30 days under laboratory conditions in two 500 L recirculating artificial seawater systems until the start of the 15-day acclimation period, in which the corals were incrementally exposed to the experimental treatment conditions.<br />
<br />
In the laboratory experiments, <em>Siderastrea siderea</em> coral specimens from each of the 18 colonies were reared for 95 days (5 August − 8 November 2011) in each of twelve 38 L glass aquaria (18 specimens per tank; 216 specimens in total) filled with artificial seawater formulated at a salinity of 35 with <em>Instant Ocean Sea Salt</em> and deionized water. Four pCO2 partial pressures [324, 477, 604, and 2553) ppm)], established by mixing pure CO2 with compressed air using <em>Aalborg</em> mass flow controllers, were bubbled with microporous ceramic airstones into the triplicate glass aquaria (12 tanks total).&nbsp; Coral specimens from each of the 18 colonies were reared in each of the 12 replicate tanks.&nbsp; The pCO2 experiments were maintained at an average temperature of 28 ºC.<br />
<br />
Experimental growth conditions for the seawater temperature experiment were similar to those for the CO2-induced ocean acidification experiment described above.&nbsp; <em>Siderastrea siderea</em> coral specimens from each of the 18 colonies were reared for 95 days (5 August − 8 November 2011) in each of nine 38 L glass aquaria (18 specimens per tank; 162 specimens in total) filled with artificial seawater formulated at a salinity of 35 with <em>Instant Ocean Sea Salt</em> and deionized water. Three experimental seawater temperatures [25, 28, and 32 ºC] were maintained in triplicate (9 tanks total) for this experiment. Coral specimens from each of the 18 colonies were reared in each of the 9 replicate tanks.&nbsp; Compressed air with an average pCO2 of 488 ppm was bubbled with microporous ceramic airstones into the triplicate glass aquaria.<br />
<br />
Coral calcification rates were estimated using a buoyant weight technique.&nbsp; <em>Siderastrea siderea</em> specimens were weighed at the beginning of the experiment and a final measurement taken at approximately 95 days. Each coral specimen was suspended by aluminum wire at 10-cm depth from a <em>Cole-Parmer</em> bottom-loading scale (precision ± 0.001; accuracy ± 0.002) in an aquarium filled with artificial seawater maintained at a temperature of 25 ºC and salinity of 33. A standardized plastic-coated zinc mass was intermittently weighed to ensure consistency of the buoyant weight method throughout the duration of the experiment.</p>
Specified by the Principal Investigator(s)
<p>These experiments utilize a combined repeated measures/split-plot design. Temperature and pCO2 represent whole-plot treatments while the reef zone of an individual coral colony represents a split-plot treatment. Hierarchical mixed-effects models were employed to account for this experimental design.&nbsp; Time measurements were treated as nested in corals, and corals were treated as nested in aquaria. Each coral's measured buoyant weight (mg) was divided by its initial surface area (cm2) to yield a normalized weight (mg cm-2). In both the pCO2 and temperature experiments normalized buoyant weight (mg cm-2) was regressed against continuous time and treatment to assess the overall effect of treatment on <em>S. siderea</em> calcification rates for the 95-day duration of the experiments. Of interest here was the coefficient of time in the regression model and the extent to which it varied by treatment. Normalized calcification rate (mg cm-2 d-1) was then obtained by extracting the regression coefficient of the continuous time variable in the model. Difference-adjusted confidence intervals were used to provide a simple graphical display of the differences among pCO2 and temperature treatment groups. All mixed models were estimated with the nlme package of R 2.15.2.</p>
<p><strong>BCO-DMO Processing Notes</strong><br />
- Generated from original files "Temp_Expt_BW_MBRSCorals.csv" contributed by Karl Castillo</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Microporous Ceramic Airstones
Microporous Ceramic Airstones
PI Supplied Instrument Name: Microporous Ceramic Airstones PI Supplied Instrument Description:In the laboratory experiments, Siderastrea siderea coral specimens from each of the 18 colonies were reared for 95 days (5 August − 8 November 2011) in each of twelve 38 L glass aquaria (18 specimens per tank; 216 specimens in total) filled with artificial seawater formulated at a salinity of 35 with Instant Ocean Sea Salt and deionized water. Four pCO2 partial pressures [324, 477, 604, and 2553) ppm)], established by mixing pure CO2 with compressed air using Aalborg mass flow controllers, were bubbled with microporous ceramic airstones into the triplicate glass aquaria (12 tanks total). Coral specimens from each of the 18 colonies were reared in each of the 12 replicate tanks. The pCO2 experiments were maintained at an average temperature of 28 ºC. Instrument Name: Airstone Instrument Short Name:Airstone Instrument Description: Airstone - Also called an aquarium bubbler, is a piece of aquarium furniture, traditionally a piece of limewood or porous stone, whose purpose is to gradually diffuse air into the tank, eliminating the noise and large bubbles of conventional air filtration systems
38 L glass aquaria
38 L glass aquaria
PI Supplied Instrument Name: 38 L glass aquaria PI Supplied Instrument Description:In the laboratory experiments, Siderastrea siderea coral specimens from each of the 18 colonies were reared for 95 days (5 August − 8 November 2011) in each of twelve 38 L glass aquaria (18 specimens per tank; 216 specimens in total) filled with artificial seawater formulated at a salinity of 35 with Instant Ocean Sea Salt and deionized water. Four pCO2 partial pressures [324, 477, 604, and 2553) ppm)], established by mixing pure CO2 with compressed air using Aalborg mass flow controllers, were bubbled with microporous ceramic airstones into the triplicate glass aquaria (12 tanks total). Coral specimens from each of the 18 colonies were reared in each of the 12 replicate tanks. The pCO2 experiments were maintained at an average temperature of 28 ºC. Instrument Name: Aquarium Instrument Short Name:Aquarium Instrument Description: Aquarium - a vivarium consisting of at least one transparent side in which water-dwelling plants or animals are kept
Aalborg Mass Flow Controller
Aalborg Mass Flow Controller
PI Supplied Instrument Name: Aalborg Mass Flow Controller PI Supplied Instrument Description:In the laboratory experiments, Siderastrea siderea coral specimens from each of the 18 colonies were reared for 95 days (5 August − 8 November 2011) in each of twelve 38 L glass aquaria (18 specimens per tank; 216 specimens in total) filled with artificial seawater formulated at a salinity of 35 with Instant Ocean Sea Salt and deionized water. Four pCO2 partial pressures [324, 477, 604, and 2553) ppm)], established by mixing pure CO2 with compressed air using Aalborg mass flow controllers, were bubbled with microporous ceramic airstones into the triplicate glass aquaria (12 tanks total). Coral specimens from each of the 18 colonies were reared in each of the 12 replicate tanks. The pCO2 experiments were maintained at an average temperature of 28 ºC. Instrument Name: Mass Flow Controller Instrument Short Name:MFC Instrument Description: Mass Flow Controller (MFC) - A device used to measure and control the flow of fluids and gases
Cole-Parmer Bottom-loading Scale
Cole-Parmer Bottom-loading Scale
PI Supplied Instrument Name: Cole-Parmer Bottom-loading Scale PI Supplied Instrument Description:Each coral specimen was suspended by aluminum wire at 10-cm depth from a Cole-Parmer bottom-loading scale (precision ± 0.001; accuracy ± 0.002 Instrument Name: scale or balance Instrument Short Name: Instrument Description: Devices that determine the mass or weight of a sample. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB13/
Deployment: lab_Ries_Sapodilla_Caye
lab_Ries_Sapodilla_Caye
Ries
laboratory
lab_Ries_Sapodilla_Caye
Justin B. Ries
University of North Carolina at Chapel Hill
http://www.unc.edu/~jries/field_sites.html
Report describing lab_Ries_Sapodilla_Caye
Deployment: lab_Ries_UNC_Chapel_Hill
lab_Ries_UNC_Chapel_Hill
Ries
laboratory
lab_Ries_UNC_Chapel_Hill
Justin B. Ries
University of North Carolina at Chapel Hill
http://www.unc.edu/~jries/index.html
Report describing lab_Ries_UNC_Chapel_Hill
Ries
laboratory