http://lod.bco-dmo.org/id/dataset/4017
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2013-08-20
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Dinitrogen fixation (N2fix) rates and nifH community structure from in situ 15N2 Gas Array from R/V Kilo Moana KM1016, KM1110 in the North Pacific Subtropical Gyre near Station ALOHA from 2010-2011 (DIAZOTROPHS-CO2 project)
2013-08-22
publication
2013-08-22
revision
BCO-DMO Linked Data URI
2013-08-22
creation
http://lod.bco-dmo.org/id/dataset/4017
Matthew J. Church
University of Hawai'i
principalInvestigator
Ricardo Letelier
Oregon State University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Church, M. J., Letelier, R. (2013) Dinitrogen fixation (N2fix) rates and nifH community structure from in situ 15N2 Gas Array from R/V Kilo Moana KM1016, KM1110 in the North Pacific Subtropical Gyre near Station ALOHA from 2010-2011 (DIAZOTROPHS-CO2 project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 22 August 2013) Version Date 2013-08-22 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/4017 [access date]
Dinitrogen fixation (N2fix) rates and nifH community structure from in situ 15N2 Gas Array Dataset Description: <p>Assessments of fixation rates of Rates of <sup>15</sup>N<sub>2</sub> and cyanobacterial nifH gene dynamics from In situ<br />
<sup>15</sup>N<sub>2</sub> Gas Array near station ALOHA deployed during KM1016 and KM1110.</p> Methods and Sampling: <p>Rates of <sup>15</sup>N<sub>2</sub> fixation were assessed using the <sup>15</sup>N isotopic tracer technique.&nbsp; Whole seawater samples from six discrete depths (5, 25, 45, 75, 100, and 125 m) were withdrawn into acid-washed duplicate 4.3 L polycarbonate bottles and sealed with caps fitted with a silicone septa. Three mL of <sup>15</sup>N<sub>2</sub> gas (98 %; Isotech Laboratories ©) was injected into each polycarbonate bottle through the silicone septum using a gas-tight syringe fitted with a stainless steel needle. The bottles were attached to a free drifting array, deployed before dawn and incubated at in situ light and temperature for ~24 hours.&nbsp; After recovery of the array, the entire volume of each bottle was filtered onto pre-combusted glass microfiber (GF/F) filters (25 mm diameter) for whole seawater N<sub>2</sub>-fixation estimates.&nbsp; All GF/F filters were placed on combusted foil pieces in Petri dishes and stored frozen at -20 °C for transport.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0850827 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0850827
completed
Matthew J. Church
University of Hawai'i
(406) 982-3301
Flathead Lake Biological Station, University of Montana 321225 Bio Station Lane
Polson
MT
59860-6815
USA
matt.church@flbs.umt.edu
pointOfContact
Ricardo Letelier
Oregon State University
541-737 3890
College of Earth, Ocean, and Atmospheric Sciences (CEOAS) Oregon State University 104 CEOAS Admin. Bldg.
Corvallis
OR
97331-5503
USA
letelier@coas.oregonstate.edu
pointOfContact
asNeeded
Dataset Version: 22 August 2013
Unknown
cruise_id
sta
date
time
lon
lat
depth
year
month
day
N2fix_mean
N2fix_diff
PP_mean
PP_std_dev
N2fix_num_obs
PP_num_obs
leuc_3H_light_incorp_mean
leuc_3H_dark_incorp_mean
leuc_3H_light_incorp_diff
leuc_3H_dark_incorp_diff
leuc_3H_light_num_obs
leuc_3H_dark_num_obs
Mass Spectrometer
theme
None, User defined
cruise id
station number
date
time of day
longitude
latitude
depth
year
month of year
day of month
No BCO-DMO term
featureType
BCO-DMO Standard Parameters
Mass Spectrometer
instrument
BCO-DMO Standard Instruments
KM1016
KM1110
service
Deployment Activity
North Pacific Subtropical Gyre near Station ALOHA (22° 45'N, 158° 00'W)
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Ocean Carbon and Biogeochemistry
http://us-ocb.org/
Ocean Carbon and Biogeochemistry
The Ocean Carbon and Biogeochemistry (OCB) program focuses on the ocean's role as a component of the global Earth system, bringing together research in geochemistry, ocean physics, and ecology that inform on and advance our understanding of ocean biogeochemistry. The overall program goals are to promote, plan, and coordinate collaborative, multidisciplinary research opportunities within the U.S. research community and with international partners. Important OCB-related activities currently include: the Ocean Carbon and Climate Change (OCCC) and the North American Carbon Program (NACP); U.S. contributions to IMBER, SOLAS, CARBOOCEAN; and numerous U.S. single-investigator and medium-size research projects funded by U.S. federal agencies including NASA, NOAA, and NSF.
The scientific mission of OCB is to study the evolving role of the ocean in the global carbon cycle, in the face of environmental variability and change through studies of marine biogeochemical cycles and associated ecosystems.
The overarching OCB science themes include improved understanding and prediction of: 1) oceanic uptake and release of atmospheric CO2 and other greenhouse gases and 2) environmental sensitivities of biogeochemical cycles, marine ecosystems, and interactions between the two.
The OCB Research Priorities (updated January 2012) include: ocean acidification; terrestrial/coastal carbon fluxes and exchanges; climate sensitivities of and change in ecosystem structure and associated impacts on biogeochemical cycles; mesopelagic ecological and biogeochemical interactions; benthic-pelagic feedbacks on biogeochemical cycles; ocean carbon uptake and storage; and expanding low-oxygen conditions in the coastal and open oceans.
OCB
largerWorkCitation
program
Oceanic diazotroph community structure and activities in a high carbon dioxide world
https://osprey.bco-dmo.org/project/2102
Oceanic diazotroph community structure and activities in a high carbon dioxide world
<p>The North Pacific Subtropical Gyre (NPSG) is the largest ocean ecosystem on Earth, playing a prominent role in global carbon cycling and forming an important reservoir of marine biodiversity. Nitrogen (N2) fixing bacteria (termed diazotrophs) provide a major source of new nitrogen to the oligotrophic waters of the NPSG, thereby exerting direct control on the carbon cycle. Oceanic uptake of CO2 causes long-term changes in the partial pressure of CO2 (pCO2) in the seawater of this ecosystem. Therefore, understanding how carbon system perturbations may influence ocean biogeochemistry is an important and timely undertaking.</p>
<p>In this project, the investigators will examine how natural assemblages of N2 fixing microorganisms respond to perturbations in seawater carbon chemistry. Laboratory and field-based experiments will be placed in the context of monthly time series measurements on the activities and abundances of N2 fixing microorganism abundances. Together, the project will provide insight into the dependence of N2 fixing microorganism physiology on variations in CO2. The broad objectives of the research are: (1) Quantify the responses and consequences of changes in seawater pCO2 on the growth and community structure of naturally-occurring assemblages of ocean diazotrophs; (2) Identify why and how changes in seawater pCO2 influence the growth and carbon acquisition strategies of two model marine diazotrophs (Trichodesmium and Crocosphaera); and (3) Quantify temporal variability in diazotroph community structure and activities at Station ALOHA.</p>
<p>This is a Collaborative Research award.</p>
DIAZOTROPHS-CO2
largerWorkCitation
project
eng; USA
oceans
North Pacific Subtropical Gyre near Station ALOHA (22° 45'N, 158° 00'W)
2013-08-22
0
BCO-DMO catalogue of parameters from Dinitrogen fixation (N2fix) rates and nifH community structure from in situ 15N2 Gas Array from R/V Kilo Moana KM1016, KM1110 in the North Pacific Subtropical Gyre near Station ALOHA from 2010-2011 (DIAZOTROPHS-CO2 project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/36028.rdf
Name: cruise_id
Units: text
Description: <p>Cruise Id</p>
http://lod.bco-dmo.org/id/dataset-parameter/36029.rdf
Name: sta
Units: integer
Description: <p>Station Id</p>
http://lod.bco-dmo.org/id/dataset-parameter/36030.rdf
Name: date
Units: YYYYMMDD
Description: <p>date sampling began</p>
http://lod.bco-dmo.org/id/dataset-parameter/36031.rdf
Name: time
Units: hhmm
Description: <p>time sampling began</p>
http://lod.bco-dmo.org/id/dataset-parameter/36032.rdf
Name: lon
Units: decimal degrees
Description: <p>longitude; negative denotes West</p>
http://lod.bco-dmo.org/id/dataset-parameter/36033.rdf
Name: lat
Units: decimal degrees
Description: <p>latitude; negative denotes South</p>
http://lod.bco-dmo.org/id/dataset-parameter/36034.rdf
Name: depth
Units: meters
Description: <p>depth of measurement</p>
http://lod.bco-dmo.org/id/dataset-parameter/36035.rdf
Name: year
Units: YYYY
Description: <p>Year of sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/36036.rdf
Name: month
Units: MM
Description: <p>month of sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/36037.rdf
Name: day
Units: DD
Description: <p>day of sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/36038.rdf
Name: N2fix_mean
Units: nanomol N/liter/day
Description: <p>Mean dinitrogen fixation rate (mean from num_obs.) bd = below detection</p>
http://lod.bco-dmo.org/id/dataset-parameter/36039.rdf
Name: N2fix_diff
Units: nanomol N/liter/day
Description: <p>Difference in dinitrogen fixation rate using N2fix_num_obs samples.</p>
http://lod.bco-dmo.org/id/dataset-parameter/36040.rdf
Name: PP_mean
Units: micrograms/liter/day
Description: <p>Primary production mean derived from num_obs samples.</p>
<p>bd = below detection</p>
http://lod.bco-dmo.org/id/dataset-parameter/36041.rdf
Name: PP_std_dev
Units: micrograms/liter/day
Description: <p>Standard deviation between Primary Production samples using PP_num_obs samples.</p>
http://lod.bco-dmo.org/id/dataset-parameter/36042.rdf
Name: N2fix_num_obs
Units: integer
Description: <p>number of samples used in calculation of dinitrogen fixation rate mean (N2fix_mean) and difference (N2fix_std_dev.)</p>
http://lod.bco-dmo.org/id/dataset-parameter/36043.rdf
Name: PP_num_obs
Units: integer
Description: <p>number of samples used in calculation of Primary Production mean (PP_mean) and standard deviation (PP_std_dev.)</p>
http://lod.bco-dmo.org/id/dataset-parameter/36044.rdf
Name: leuc_3H_light_incorp_mean
Units: picomole/liter/hour
Description: <p>3H-leucine (Light) incorporations rates in picomoles Leucine(Light) per liter per hour</p>
http://lod.bco-dmo.org/id/dataset-parameter/36045.rdf
Name: leuc_3H_dark_incorp_mean
Units: picomole/liter/hour
Description: <p>Mean 3H-Leucine (Dark) incorporation rates in picomoles Leucine (Dark) per liter hour using Leucine_3H_Dark_num_obs samples.</p>
http://lod.bco-dmo.org/id/dataset-parameter/36046.rdf
Name: leuc_3H_light_incorp_diff
Units: picomole/liter/hour
Description: <p>Standard deviation between leuc_3H_num_obs samples of 3H-Leucine (light) incorporation rate.</p>
http://lod.bco-dmo.org/id/dataset-parameter/36047.rdf
Name: leuc_3H_dark_incorp_diff
Units: picomole/liter/hour
Description: <p>Standard deviation between leuc_3H_num_obs samples of 3H-Leucine (dark) incorporation rate.</p>
http://lod.bco-dmo.org/id/dataset-parameter/36048.rdf
Name: leuc_3H_light_num_obs
Units: integer
Description: <p>Number of observations used in calculation of leuc_3H_light_incorp mean and standard deviation.</p>
http://lod.bco-dmo.org/id/dataset-parameter/36049.rdf
Name: leuc_3H_dark_num_obs
Units: integer
Description: <p>Number of observations used in calculation of leuc_3H_dark_incorp mean and standard deviation.</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
4145
https://datadocs.bco-dmo.org/file/N77Grz5hM21Z0n/N2fix_array.csv
N2fix_array.csv
Primary data file for dataset ID 4017
download
https://osprey.bco-dmo.org/dataset/4017/data/download
download
onLine
dataset
<p>Rates of <sup>15</sup>N<sub>2</sub> fixation were assessed using the <sup>15</sup>N isotopic tracer technique.&nbsp; Whole seawater samples from six discrete depths (5, 25, 45, 75, 100, and 125 m) were withdrawn into acid-washed duplicate 4.3 L polycarbonate bottles and sealed with caps fitted with a silicone septa. Three mL of <sup>15</sup>N<sub>2</sub> gas (98 %; Isotech Laboratories ©) was injected into each polycarbonate bottle through the silicone septum using a gas-tight syringe fitted with a stainless steel needle. The bottles were attached to a free drifting array, deployed before dawn and incubated at in situ light and temperature for ~24 hours.&nbsp; After recovery of the array, the entire volume of each bottle was filtered onto pre-combusted glass microfiber (GF/F) filters (25 mm diameter) for whole seawater N<sub>2</sub>-fixation estimates.&nbsp; All GF/F filters were placed on combusted foil pieces in Petri dishes and stored frozen at -20 °C for transport.</p>
Specified by the Principal Investigator(s)
<p>See:&nbsp; <a href="http://data.bco-dmo.org/DIAZOTROPHS_CO2/N2&amp;nifH_methods.pdf">Dinitrogen fixation and nifH analysis methods</a>.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Mass Spectrometer
Mass Spectrometer
PI Supplied Instrument Name: Mass Spectrometer Instrument Name: Mass Spectrometer Instrument Short Name:Mass Spec Instrument Description: General term for instruments used to measure the mass-to-charge ratio of ions; generally used to find the composition of a sample by generating a mass spectrum representing the masses of sample components. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
Cruise: KM1016
KM1016
R/V Kilo Moana
Community Standard Description
International Council for the Exploration of the Sea
R/V Kilo Moana
vessel
KM1016
Matthew J. Church
University of Hawai'i
http://dmoserv3.bco-dmo.org/jg/serv/BCO-DMO/DIAZOTROPHS_CO2/726342.html1%7Bdir=dmoserv3.whoi.edu/jg/dir/BCO-DMO/DIAZOTROPHS_CO2/,info=dmoserv3.bco-dmo.org/jg/info/BCO-DMO/DIAZOTROPHS_CO2/CO2_experimental%7D?cruise_id_eq_km1016
Report describing KM1016
Cruise: KM1110
KM1110
R/V Kilo Moana
Community Standard Description
International Council for the Exploration of the Sea
R/V Kilo Moana
vessel
KM1110
Matthew J. Church
University of Hawai'i
http://dmoserv3.bco-dmo.org/jg/serv/BCO-DMO/DIAZOTROPHS_CO2/726342.html1%7Bdir=dmoserv3.whoi.edu/jg/dir/BCO-DMO/DIAZOTROPHS_CO2/,info=dmoserv3.bco-dmo.org/jg/info/BCO-DMO/DIAZOTROPHS_CO2/CO2_experimental%7D?cruise_id_eq_km1110
Report describing KM1110
R/V Kilo Moana
Community Standard Description
International Council for the Exploration of the Sea
R/V Kilo Moana
vessel