http://lod.bco-dmo.org/id/dataset/4046
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2013-09-20
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
1A: Partitioning of carbon as a function of pCO2 and temperature during growth of Thalassiosira weissflogii from UCSB Marine Science Institute Passow Lab from 2009 to 2010 (OA - Effects of High CO2 project)
2013-09-19
publication
2013-09-19
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-08-28
publication
https://doi.org/10.1575/1912/bco-dmo.4046.1
Uta Passow
University of California-Santa Barbara
principalInvestigator
Mark A. Brzezinski
University of California-Santa Barbara
principalInvestigator
Craig A. Carlson
University of California-Santa Barbara
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Passow, U., Brzezinski, M., Carlson, C. (2013) Partitioning of carbon as a function of pCO2 and temperature during growth of Thalassiosira weissflogii from UCSB Marine Science Institute Passow Lab from 2009 to 2010 (OA - Effects of High CO2 project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2013-09-19 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.4046.1 [access date]
Data Set #1A: Partitioning of carbon as a function of pCO2 and temperature during growth of Thalassiosira weissflogii Dataset Description: <p>Experiments with the diatom <em>Thalassiosira weissflogii</em> (CCMP 1336) on impact of temperature and carbonate chemistry on carbon uptake and partitioning into particulate and dissolved organic matter</p>
<p>Experiments conducted in March and May 2013 in Santa Barbara California, Passow lab<br />
Treatments: mulitfactorial analysis with 2 temperature treatments (15, 20C) and two ocean acidification treatments (400 and 1000 µatm)<br />
<br />
Daily sampling after light cycle (14/10) was completed</p>
<p>See related publication Thaucher et al (2015).</p> Methods and Sampling: <p><strong>Methods:</strong><br />
Two experiments, one with <em>Thalassiosira weissflogii</em> and one with <em>Dacyliosolen fragilissimus</em> were conducted. Replicates of each treatment (2 temperatures by 2 pCO2 conditions) were grown in eight 20 L gas-tight polyethylene bags. <em>T. weissflogii</em> and <em>D. fragilissimus</em>, respectively, were grown in artificial and natural seawater based, modified f/2 media. Initial nitrate addition was 15 µmol L-1 NO3 for both species, and 6 and 8 µmol L-1 PO4, and 16 and 50 µmol L-1 SiO3 for <em>T. weissflogii</em> and <em>D. fragilissimus</em>, respectively. Trace metals and vitamins were added according to f/8.&nbsp; Cultures were grown under a light / dark cycle of 14/10 hours at ~90 – 100 µE m-2 s-1 at 15 °C and 20 °C. Partial pressure levels of CO2 were set without bubbling to 400 and 1000 µatm for both temperatures; by appropriate addition of HCO3 and HCl.<br />
<br />
Prior to the experiments the diatoms were acclimatized to the respective target conditions growing semi-continuously in gas-tight polycarbonate bottles for at least a week. At the onset of the experiment bags were inoculated with ~1000 cells ml-1 for <em>T. weissflogii</em> and ~500 cells ml-1 for the larger <em>D. fragilissimus</em>. Daily sampling conducted immediately after the end of each light cycle, was continued for at least 6 days after NO3 depletion.<br />
<br />
For the determination of particulate organic carbon and nitrogen (POC and PON), samples were filtered onto precombusted (5 hours at 450 °C) glassfibre filters (Whatman, GF/F, 0.7 µm nominal poresize), dried at ~60 °C for 24 hours and analyzed on a CHN organic elemental analyzer (Control Equipment Corp., CEC 440HA). Samples for dissolved inorganic nitrate, nitrite, phosphate and silicate were filtered through 0.2 µm filters and measured on a flow injection analyzer (Lachat Instruments Div., QuikChem 8000). Samples for dissolved organic carbon (DOC) were gravity-filtered through precombusted GF/F filters, with the filtrate being collecting collected in acid-washed (HCl, 10%) and precombusted glass vials and frozen (at -20 °C). The analysis was carried out via high temperature combustion on a modified Shimadzu TOC-V analyzers. Dissolved inorganic carbon (DIC) was measured on a non-dispersive infrared (NDIR) analyzer. Samples were filtered through glassfibre filters (GF/F) and stored in gas-tight ~400 ml borosilicate bottles until analysis.<br />
<br />
pH (total scale) was measured spetrophotometrically at 25ºC (Thermo Scientific Genesys 105 VIS Spetrophotometer with a SPG 1A air-cooled single cell Peltier element), using m-cresol as an indicator dye. The dye was calibrated against certified reference material (A. Dickson, La Jolla, California). Samples for transparent exopolymer particles (TEP) were filtered onto 0.4 µm polycarbonate filters (Poretics) and subsequently stained with Alcian Blue following the procedure of Passow and Alldredge (1995).</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1041038 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1041038
completed
Uta Passow
University of California-Santa Barbara
709-864-8010
Ocean Sciences Centre, Memorial University Marine Lab Road, Logy Bay
St. John's
Newfoundland
A1C 5S7
Canada
uta.passow@mun.ca
pointOfContact
Mark A. Brzezinski
University of California-Santa Barbara
805-893-7061
UCSB Marine Science Institute Bldg 520 Rm 4002 Fl 4L
Santa Barbara
CA
93106
USA
mark.brzezinski@lifesci.ucsb.edu
pointOfContact
Craig A. Carlson
University of California-Santa Barbara
(805) 893-2541
University of California Department of Ecology, Evolution and Marine Biology
Santa Barbara
CA
93106-6150
USA
craig_carlson@ucsb.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Lab_Id
Lat
Lon
Temp
pCO2
replicate
sampling
pH_at_25C
DIC
fluorescence
NO3
PO4
Si
POC
PON
DOC
DON
TEP
bacteria_production
Control Equipment Corp., CEC 440HA
Lachat Instruments Div., QuikChem 8000
NDIR Gas Analyzer
Thermo Scientific Genesys 105 VIS Spetrophotometer with a SPG 1A air-cooled single cell Peltier element
Shimadzu TOC-V Analyzer
theme
None, User defined
laboratory
latitude
longitude
water temperature at measurement depth
No BCO-DMO term
featureType
BCO-DMO Standard Parameters
CHN Elemental Analyzer
Flow Injection Analyzer
LI-COR LI-840 Gas Analyzer
pH Sensor
Shimadzu TOC-V Analyzer
instrument
BCO-DMO Standard Instruments
lab_UCSB_MSI_Passow
service
Deployment Activity
Passow Lab, Marine Science Institute, University of California Santa Barbara
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA)
https://www.nsf.gov/funding/pgm_summ.jsp?pims_id=503477
Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA)
NSF Climate Research Investment (CRI) activities that were initiated in 2010 are now included under Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES). SEES is a portfolio of activities that highlights NSF's unique role in helping society address the challenge(s) of achieving sustainability. Detailed information about the SEES program is available from NSF (https://www.nsf.gov/funding/pgm_summ.jsp?pims_id=504707).
In recognition of the need for basic research concerning the nature, extent and impact of ocean acidification on oceanic environments in the past, present and future, the goal of the SEES: OA program is to understand (a) the chemistry and physical chemistry of ocean acidification; (b) how ocean acidification interacts with processes at the organismal level; and (c) how the earth system history informs our understanding of the effects of ocean acidification on the present day and future ocean.
Solicitations issued under this program:
NSF 10-530, FY 2010-FY2011
NSF 12-500, FY 2012
NSF 12-600, FY 2013
NSF 13-586, FY 2014
NSF 13-586 was the final solicitation that will be released for this program.
PI Meetings:
1st U.S. Ocean Acidification PI Meeting(March 22-24, 2011, Woods Hole, MA)
2nd U.S. Ocean Acidification PI Meeting(Sept. 18-20, 2013, Washington, DC)
3rd U.S. Ocean Acidification PI Meeting (June 9-11, 2015, Woods Hole, MA – Tentative)
NSF media releases for the Ocean Acidification Program:
Press Release 10-186 NSF Awards Grants to Study Effects of Ocean Acidification
Discovery Blue Mussels "Hang On" Along Rocky Shores: For How Long?
Discovery nsf.gov - National Science Foundation (NSF) Discoveries - Trouble in Paradise: Ocean Acidification This Way Comes - US National Science Foundation (NSF)
Press Release 12-179 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: Finding New Answers Through National Science Foundation Research Grants - US National Science Foundation (NSF)
Press Release 13-102 World Oceans Month Brings Mixed News for Oysters
Press Release 13-108 nsf.gov - National Science Foundation (NSF) News - Natural Underwater Springs Show How Coral Reefs Respond to Ocean Acidification - US National Science Foundation (NSF)
Press Release 13-148 Ocean acidification: Making new discoveries through National Science Foundation research grants
Press Release 13-148 - Video nsf.gov - News - Video - NSF Ocean Sciences Division Director David Conover answers questions about ocean acidification. - US National Science Foundation (NSF)
Press Release 14-010 nsf.gov - National Science Foundation (NSF) News - Palau's coral reefs surprisingly resistant to ocean acidification - US National Science Foundation (NSF)
Press Release 14-116 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: NSF awards $11.4 million in new grants to study effects on marine ecosystems - US National Science Foundation (NSF)
SEES-OA
largerWorkCitation
program
Ocean Carbon and Biogeochemistry
http://us-ocb.org/
Ocean Carbon and Biogeochemistry
The Ocean Carbon and Biogeochemistry (OCB) program focuses on the ocean's role as a component of the global Earth system, bringing together research in geochemistry, ocean physics, and ecology that inform on and advance our understanding of ocean biogeochemistry. The overall program goals are to promote, plan, and coordinate collaborative, multidisciplinary research opportunities within the U.S. research community and with international partners. Important OCB-related activities currently include: the Ocean Carbon and Climate Change (OCCC) and the North American Carbon Program (NACP); U.S. contributions to IMBER, SOLAS, CARBOOCEAN; and numerous U.S. single-investigator and medium-size research projects funded by U.S. federal agencies including NASA, NOAA, and NSF.
The scientific mission of OCB is to study the evolving role of the ocean in the global carbon cycle, in the face of environmental variability and change through studies of marine biogeochemical cycles and associated ecosystems.
The overarching OCB science themes include improved understanding and prediction of: 1) oceanic uptake and release of atmospheric CO2 and other greenhouse gases and 2) environmental sensitivities of biogeochemical cycles, marine ecosystems, and interactions between the two.
The OCB Research Priorities (updated January 2012) include: ocean acidification; terrestrial/coastal carbon fluxes and exchanges; climate sensitivities of and change in ecosystem structure and associated impacts on biogeochemical cycles; mesopelagic ecological and biogeochemical interactions; benthic-pelagic feedbacks on biogeochemical cycles; ocean carbon uptake and storage; and expanding low-oxygen conditions in the coastal and open oceans.
OCB
largerWorkCitation
program
Will high CO2 conditions affect production, partitioning and fate of organic matter?
http://www.msi.ucsb.edu/people/research-scientists/uta-passow
Will high CO2 conditions affect production, partitioning and fate of organic matter?
<p><strong>From the NSF Award Abstract</strong></p>
<p>Coastal waters are already experiencing episodic exposure to carbonate conditions that were not expected until the end of the century making understanding the response to these episodic events as important as understanding the long-term mean response. Among the most striking examples are those associated with coastal upwelling along the west coast of the US, where the pH of surface waters may drop to 7.6 and pCO2 can reach 1100 uatm. Upwelling systems are responsible for a significant fraction of global carbon export making them prime targets for investigations on how ocean acidification is already affecting the biological pump today.</p>
<p>In this study, researchers at the University of California at Santa Barbara will investigate the potential effects of ocean acidification on the strength of the biological pump under the transient increases in CO2 experienced due to upwelling. Increases in CO2 are expected to alter the path and processing of carbon through marine food webs thereby strengthening the biological pump. Increases in inorganic carbon without proportional increases in nutrients result in carbon over-consumption by phytoplankton. How carbon over-consumption affects the strength of the biological pump will depend on the fate of the extra carbon that is either incorporated into phytoplankton cells forming particulate organic matter (POM), or is excreted as dissolved organic matter (DOM). Results from mesocosm experiments demonstrate that the mechanisms controlling the partitioning of fixed carbon between the particulate and dissolved phases, and the processing of those materials, are obscured when both processes operate simultaneously under natural or semi-natural conditions. Here, POM and DOM production and the heterotrophic processing of these materials will be separated experimentally across a range of CO2 concentrations by conducting basic laboratory culture experiments. In this way the mechanisms whereby elevated CO2 alters the flow of carbon along these paths can be elucidated and better understood for use in mechanistic forecasting models.</p>
<p>Broader Impacts- The need to understand the effects of ocean acidification for the future of society is clear. In addition to research education, both formal and informal, will be important for informing the public. Within this project 1-2 graduate students and 2-3 minority students will be recruited as interns from the CAMP program (California Alliance for Minority Participation). Within the 'Ocean to Classrooms' program run by outreach personnel from UCSB's Marine Science Institute an educational unit for K-12 students will be developed. Advice and support is also given to the Education Coordinator of NOAA, Channel Islands National Marine Sanctuary for the development of an education unit on ocean acidification.</p>
<p>
<strong>PUBLICATIONS PRODUCED AS A RESULT OF THIS RESEARCH</strong></p>
<p>Arnosti C, Grossart H-P, Muehling M, Joint I, Passow U. "Dynamics of extracellular enzyme activities in seawater under changed atmsopheric pCO2: A mesocosm investigation.," Aquatic Microbial Ecology, v.64, 2011, p. 285.</p>
<p>Passow U. "The Abiotic Formation of TEP under Ocean Acidification Scenarios.," Marine Chemistry, v.128-129, 2011, p. 72.</p>
<p>Passow, Uta; Carlson, Craig A.. "The biological pump in a high CO2 world," MARINE ECOLOGY PROGRESS SERIES, v.470, 2012, p. 249-271.</p>
<p>Gaerdes, Astrid; Ramaye, Yannic; Grossart, Hans-Peter; Passow, Uta; Ullrich, Matthias S.. "Effects of Marinobacter adhaerens HP15 on polymer exudation by Thalassiosira weissflogii at different N:P ratios," MARINE ECOLOGY PROGRESS SERIES, v.461, 2012, p. 1-14.</p>
<p>Philip Boyd, Tatiana Rynearson, Evelyn Armstrong, Feixue Fu, Kendra Hayashi, Zhangi Hu, David Hutchins, Raphe Kudela, Elena Litchman, Margaret Mulholland, Uta Passow, Robert Strzepek, Kerry Whittaker, Elizabeth Yu, Mridul Thomas. "Marine Phytoplankton Temperature versus Growth Responses from Polar to Tropical Waters - Outcome of a Scientific Community-Wide Study," PLOS One 8, v.8, 2013, p. e63091.</p>
<p>Arnosti, C., B. M. Fuchs, R. Amann, and U. Passow. "Contrasting extracellular enzyme activities of particle-associated bacteria from distinct provinces of the North Atlantic Ocean," Frontiers in Microbiology, v.3, 2012, p. 1.</p>
<p>Koch, B.P., Kattner, G., Witt, M., Passow, U., 2014. Molecular insights into the microbial formation of marine dissolved organic matter: recalcitrant or labile? Biogeosciences Discuss. 11 (2), 3065-3111.</p>
<p>Taucher, J., Brzezinski, M., Carlson, C., James, A., Jones, J., Passow, U., Riebesell, U., submitted. Effects of warming and elevated pCO2 on carbon uptake and partitioning of the marine diatoms Thalassiosira weissflogii and Dactyliosolen fragilissimus. Limnology and Oceanography</p>
OA - Effects of High CO2
largerWorkCitation
project
eng; USA
oceans
Passow Lab, Marine Science Institute, University of California Santa Barbara
-119.842
-119.842
34.4126
34.4126
2013-03-01
2013-05-31
Passow Lab, Marine Science Institute, University of California Santa Barbara
0
BCO-DMO catalogue of parameters from 1A: Partitioning of carbon as a function of pCO2 and temperature during growth of Thalassiosira weissflogii from UCSB Marine Science Institute Passow Lab from 2009 to 2010 (OA - Effects of High CO2 project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/36529.rdf
Name: Lab_Id
Units: text
Description: <p>Lab Id - Lab identifier where experiments were conducted</p>
http://lod.bco-dmo.org/id/dataset-parameter/36530.rdf
Name: Lat
Units: decimal degrees
Description: <p>Approximate Latitude Position of Lab; South is negative</p>
http://lod.bco-dmo.org/id/dataset-parameter/36531.rdf
Name: Lon
Units: decimal degrees
Description: <p>Approximate Longitude Position of Lab; West is negative</p>
http://lod.bco-dmo.org/id/dataset-parameter/36532.rdf
Name: Temp
Units: Degrees C
Description: <p>Temperature</p>
http://lod.bco-dmo.org/id/dataset-parameter/36533.rdf
Name: pCO2
Units: micro atm
Description: <p>pCO2 conditions</p>
http://lod.bco-dmo.org/id/dataset-parameter/36534.rdf
Name: replicate
Units: dimensionless
Description: <p>number of replicate samples</p>
http://lod.bco-dmo.org/id/dataset-parameter/36535.rdf
Name: sampling
Units: dimensionless
Description: <p>day of experiment</p>
http://lod.bco-dmo.org/id/dataset-parameter/36536.rdf
Name: pH_at_25C
Units: total scale
Description: <p>pH at 25 C</p>
http://lod.bco-dmo.org/id/dataset-parameter/36537.rdf
Name: DIC
Units: micro mol C L-1
Description: <p>DIC</p>
http://lod.bco-dmo.org/id/dataset-parameter/36538.rdf
Name: fluorescence
Units: (tbd)
Description: <p>Fluorescence - Instantaneous Chlorophyll Fluorescence (FT from AquaPen)</p>
http://lod.bco-dmo.org/id/dataset-parameter/36539.rdf
Name: NO3
Units: micro mol N L-1
Description: <p>NO3</p>
http://lod.bco-dmo.org/id/dataset-parameter/36540.rdf
Name: PO4
Units: micro mol P L-1
Description: <p>PO4</p>
http://lod.bco-dmo.org/id/dataset-parameter/36541.rdf
Name: Si
Units: micro mol Si L-1
Description: <p>Si</p>
http://lod.bco-dmo.org/id/dataset-parameter/36542.rdf
Name: POC
Units: micro mol C L-1
Description: <p>POC</p>
http://lod.bco-dmo.org/id/dataset-parameter/36543.rdf
Name: PON
Units: micro mol N L-1
Description: <p>PON</p>
http://lod.bco-dmo.org/id/dataset-parameter/36544.rdf
Name: DOC
Units: micro mol C L-1
Description: <p>DOC</p>
http://lod.bco-dmo.org/id/dataset-parameter/36545.rdf
Name: DON
Units: micro mol N L-1
Description: <p>DON</p>
http://lod.bco-dmo.org/id/dataset-parameter/36546.rdf
Name: TEP
Units: GXEQ L-1
Description: <p>TEP</p>
http://lod.bco-dmo.org/id/dataset-parameter/36547.rdf
Name: bacteria_production
Units: pmol leucine L-1 hr-1
Description: <p>Bacteria production</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
9682
https://darchive.mblwhoilibrary.org/bitstream/1912/24474/1/dataset-4046_data-set-1a-partitioning-carbon-function-pco2-and-temperature-during-growth-thalassiosira__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.4046.1
download
onLine
dataset
<p><strong>Methods:</strong><br />
Two experiments, one with <em>Thalassiosira weissflogii</em> and one with <em>Dacyliosolen fragilissimus</em> were conducted. Replicates of each treatment (2 temperatures by 2 pCO2 conditions) were grown in eight 20 L gas-tight polyethylene bags. <em>T. weissflogii</em> and <em>D. fragilissimus</em>, respectively, were grown in artificial and natural seawater based, modified f/2 media. Initial nitrate addition was 15 µmol L-1 NO3 for both species, and 6 and 8 µmol L-1 PO4, and 16 and 50 µmol L-1 SiO3 for <em>T. weissflogii</em> and <em>D. fragilissimus</em>, respectively. Trace metals and vitamins were added according to f/8.&nbsp; Cultures were grown under a light / dark cycle of 14/10 hours at ~90 – 100 µE m-2 s-1 at 15 °C and 20 °C. Partial pressure levels of CO2 were set without bubbling to 400 and 1000 µatm for both temperatures; by appropriate addition of HCO3 and HCl.<br />
<br />
Prior to the experiments the diatoms were acclimatized to the respective target conditions growing semi-continuously in gas-tight polycarbonate bottles for at least a week. At the onset of the experiment bags were inoculated with ~1000 cells ml-1 for <em>T. weissflogii</em> and ~500 cells ml-1 for the larger <em>D. fragilissimus</em>. Daily sampling conducted immediately after the end of each light cycle, was continued for at least 6 days after NO3 depletion.<br />
<br />
For the determination of particulate organic carbon and nitrogen (POC and PON), samples were filtered onto precombusted (5 hours at 450 °C) glassfibre filters (Whatman, GF/F, 0.7 µm nominal poresize), dried at ~60 °C for 24 hours and analyzed on a CHN organic elemental analyzer (Control Equipment Corp., CEC 440HA). Samples for dissolved inorganic nitrate, nitrite, phosphate and silicate were filtered through 0.2 µm filters and measured on a flow injection analyzer (Lachat Instruments Div., QuikChem 8000). Samples for dissolved organic carbon (DOC) were gravity-filtered through precombusted GF/F filters, with the filtrate being collecting collected in acid-washed (HCl, 10%) and precombusted glass vials and frozen (at -20 °C). The analysis was carried out via high temperature combustion on a modified Shimadzu TOC-V analyzers. Dissolved inorganic carbon (DIC) was measured on a non-dispersive infrared (NDIR) analyzer. Samples were filtered through glassfibre filters (GF/F) and stored in gas-tight ~400 ml borosilicate bottles until analysis.<br />
<br />
pH (total scale) was measured spetrophotometrically at 25ºC (Thermo Scientific Genesys 105 VIS Spetrophotometer with a SPG 1A air-cooled single cell Peltier element), using m-cresol as an indicator dye. The dye was calibrated against certified reference material (A. Dickson, La Jolla, California). Samples for transparent exopolymer particles (TEP) were filtered onto 0.4 µm polycarbonate filters (Poretics) and subsequently stained with Alcian Blue following the procedure of Passow and Alldredge (1995).</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing Notes</strong><br />
Original file: "Data_Mastersheet_TWeiss_DactyFrag_2013_JT.xlsx"&nbsp; contributed by Uta Passow<br />
Sheet: "T. weiss 2013"<br />
- Approx Lat/Lon of Passow Lab appended to enable data discovery in MapServer<br />
- "nd" (no data) inserted into blank cells<br />
- Parameter names edited to conform to BCO-DMO parameter naming conventions</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Control Equipment Corp., CEC 440HA
Control Equipment Corp., CEC 440HA
PI Supplied Instrument Name: Control Equipment Corp., CEC 440HA PI Supplied Instrument Description:For the determination of particulate organic carbon and nitrogen (POC and PON), samples were filtered onto precombusted (5 hours at 450 °C) glassfibre filters (Whatman, GF/F, 0.7 µm nominal poresize), dried at ~60 °C for 24 hours and analyzed on a CHN organic elemental analyzer (Control Equipment Corp., CEC 440HA) Instrument Name: CHN Elemental Analyzer Instrument Short Name:CHN_EA Instrument Description: A CHN Elemental Analyzer is used for the determination of carbon, hydrogen, and nitrogen content in organic and other types of materials, including solids, liquids, volatile, and viscous samples.
Lachat Instruments Div., QuikChem 8000
Lachat Instruments Div., QuikChem 8000
PI Supplied Instrument Name: Lachat Instruments Div., QuikChem 8000 PI Supplied Instrument Description:Samples for dissolved inorganic nitrate, nitrite, phosphate and silicate were filtered through 0.2 µm filters and measured on a flow injection analyzer (Lachat Instruments Div., QuikChem 8000). Instrument Name: Flow Injection Analyzer Instrument Short Name:FIA Instrument Description: An instrument that performs flow injection analysis. Flow injection analysis (FIA) is an approach to chemical analysis that is accomplished by injecting a plug of sample into a flowing carrier stream. FIA is an automated method in which a sample is injected into a continuous flow of a carrier solution that mixes with other continuously flowing solutions before reaching a detector. Precision is dramatically increased when FIA is used instead of manual injections and as a result very specific FIA systems have been developed for a wide array of analytical techniques. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB36/
NDIR Gas Analyzer
NDIR Gas Analyzer
PI Supplied Instrument Name: NDIR Gas Analyzer PI Supplied Instrument Description:Dissolved inorganic carbon (DIC) was measured on a non-dispersive infrared (NDIR) analyzer. Instrument Name: LI-COR LI-840 Gas Analyzer Instrument Short Name:LI-840 Instrument Description: The LI-COR LI-840 is an absolute, non-dispersive infrared gas analyzer based on a single path, dual wavelength, and thermostatically controlled infrared detection system. It has an operating temperature range of -20 to +40 degrees Celsius. CO2 is measured in the range 0-3,000 ppm with an accuracy of better than 1.5 percent of the reading. H2O is measured in the range 0-80 ppt with an accuracy of better than 1.5 percent of reading. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/382/
Thermo Scientific Genesys 105 VIS Spetrophotometer with a SPG 1A air-cooled single cell Peltier element
Thermo Scientific Genesys 105 VIS Spetrophotometer with a SPG 1A air-cooled single cell Peltier element
PI Supplied Instrument Name: Thermo Scientific Genesys 105 VIS Spetrophotometer with a SPG 1A air-cooled single cell Peltier element PI Supplied Instrument Description:pH (total scale) was measured spetrophotometrically at 25ºC (Thermo Scientific Genesys 105 VIS Spetrophotometer with a SPG 1A air-cooled single cell Peltier element), using m-cresol as an indicator dye. Instrument Name: pH Sensor Instrument Short Name:pH Sensor Instrument Description: An instrument that measures the hydrogen ion activity in solutions.
The overall concentration of hydrogen ions is inversely related to its pH. The pH scale ranges from 0 to 14 and indicates whether acidic (more H+) or basic (less H+).
Shimadzu TOC-V Analyzer
Shimadzu TOC-V Analyzer
PI Supplied Instrument Name: Shimadzu TOC-V Analyzer PI Supplied Instrument Description:Samples for dissolved organic carbon (DOC) were gravity-filtered through precombusted GF/F filters, with the filtrate being collecting collected in acid-washed (HCl, 10%) and precombusted glass vials and frozen (at -20 °C). The analysis was carried out via high temperature combustion on a modified Shimadzu TOC-V analyzers. Instrument Name: Shimadzu TOC-V Analyzer Instrument Short Name:Shimadzu TOC-V Instrument Description: A Shimadzu TOC-V Analyzer measures DOC by high temperature combustion method. Community Standard Description: http://onto.nerc.ac.uk/CAST/124
Deployment: lab_UCSB_MSI_Passow
lab_UCSB_MSI_Passow
UCSB MSI Passow
UCSB MSI Passow
laboratory
lab_UCSB_MSI_Passow
Uta Passow
University of California-Santa Barbara
http://www.msi.ucsb.edu/people/research-scientists/uta-passow
Report describing lab_UCSB_MSI_Passow
UCSB MSI Passow
UCSB MSI Passow
laboratory