http://lod.bco-dmo.org/id/dataset/4056
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2013-10-10
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Urchin Fertilization Studies from Levin laboratory at Scripps Institute of Oceanography from 2009 to 2012 (SeapHOx project)
2013-10-10
publication
2013-10-10
revision
BCO-DMO Linked Data URI
2013-10-10
creation
http://lod.bco-dmo.org/id/dataset/4056
Christina Frieder
University of California-San Diego Scripps
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Frieder, C. (2013) Urchin Fertilization Studies from Levin laboratory at Scripps Institute of Oceanography from 2009 to 2012 (SeapHOx project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 10 October 2013) Version Date 2013-10-10 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/4056 [access date]
Urchin Fertilization Studies Dataset Description: <p>This dataset presents fertilization success of <em>Strongylocentrotus franciscanus </em>and <em>S. purpuratus </em>from experiments that manipulated pH, sperm-egg ratio, and sire identity.</p>
<p><strong>Related files and references:</strong><br />
Frieder CA (2013) Evaluating low oxygen and pH variation and its effects on invertebrate early life stages on upwelling margins. Dissertation. University of California San Diego.</p> Methods and Sampling: <p><strong>Sampling and Analytical Methodology:</strong><br />
Spawning was induced by injecting 0.55 M KCl through the peristomal membrane. Oocytes were collected in dishes of filtered seawater (FSW, 0.22 µm). Number of adults used in each experiment ranged from 2 – 5 females and 3 – 5 males. Sperm were collected dry from the gonopores of males and placed in a small vial and kept on ice until use. A 0.1% dilution from dry sperm was made to verify sperm motility, and then preserved in formalin to count sperm densities with a hemacytometer in order to calculate the amount of diluted sperm required to attain desired sperm-egg ratios and sperm densities for each treatment. Oocytes from multiple females were mixed and egg densities determined by counting 8 x 20 µl aliquots. Eggs were added to experimental beakers at a density of 5 eggs ml<sup>-1</sup>, and incubated in experimental conditions for 10 min before sperm were added. Dry sperm was diluted immediately before addition to experimental beakers. Following sperm addition the seawater in each replicate was gently stirred. Fertilization proceeded for 20 min and was arrested by transferring the contents of each replicate to a 1% formalin solution. The fertilization ratio, defined as the proportion of eggs fertilized, was determined by counting the frequency of occurrence of a fertilization envelope in at least 100 eggs per replicate. All experiments were carried out in the Scripps experimental aquarium facility during the spring of 2013. 500 ml glass beakers were used, and there were three replicates per treatment level.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0927445 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0927445
completed
Christina Frieder
University of California-San Diego Scripps
(858)534-3579
9500 Gilman Drive MC0218
La Jolla
CA
92093
USA
ctanner@ucsd.edu
pointOfContact
asNeeded
Dataset Version: 10 October 2013
Unknown
Lab_Id
Latitude
Longitude
Expt
Species
pH
Sperm2Egg_Ratio
Sire_Identity
Rep
Fertilization_Ratio
Hemocytometer
theme
None, User defined
laboratory
latitude
longitude
experiment id
species
pH
No BCO-DMO term
replicate
featureType
BCO-DMO Standard Parameters
Hemocytometer
instrument
BCO-DMO Standard Instruments
lab_SIO_Levin
service
Deployment Activity
9500 Gilman Drive La Jolla, California 92093-0218
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Ocean Carbon and Biogeochemistry
http://us-ocb.org/
Ocean Carbon and Biogeochemistry
The Ocean Carbon and Biogeochemistry (OCB) program focuses on the ocean's role as a component of the global Earth system, bringing together research in geochemistry, ocean physics, and ecology that inform on and advance our understanding of ocean biogeochemistry. The overall program goals are to promote, plan, and coordinate collaborative, multidisciplinary research opportunities within the U.S. research community and with international partners. Important OCB-related activities currently include: the Ocean Carbon and Climate Change (OCCC) and the North American Carbon Program (NACP); U.S. contributions to IMBER, SOLAS, CARBOOCEAN; and numerous U.S. single-investigator and medium-size research projects funded by U.S. federal agencies including NASA, NOAA, and NSF.
The scientific mission of OCB is to study the evolving role of the ocean in the global carbon cycle, in the face of environmental variability and change through studies of marine biogeochemical cycles and associated ecosystems.
The overarching OCB science themes include improved understanding and prediction of: 1) oceanic uptake and release of atmospheric CO2 and other greenhouse gases and 2) environmental sensitivities of biogeochemical cycles, marine ecosystems, and interactions between the two.
The OCB Research Priorities (updated January 2012) include: ocean acidification; terrestrial/coastal carbon fluxes and exchanges; climate sensitivities of and change in ecosystem structure and associated impacts on biogeochemical cycles; mesopelagic ecological and biogeochemical interactions; benthic-pelagic feedbacks on biogeochemical cycles; ocean carbon uptake and storage; and expanding low-oxygen conditions in the coastal and open oceans.
OCB
largerWorkCitation
program
Macrophyte-induced variability in coastal ocean pH and consequences for invertebrate larvae
https://osprey.bco-dmo.org/project/2122
Macrophyte-induced variability in coastal ocean pH and consequences for invertebrate larvae
<p>Increased concentrations of atmospheric carbon dioxide are acidifying the marine environment at unprecedented rates. However, relative to the open ocean, predictions of ocean acidification for the coastal ocean are confounded by the greater inherent variability of carbonate chemistry which includes macrophyte photosynthesis and respiration. This proposal addresses the interplay between anthropogenically driven pH changes and the inherently variable coastal ocean carbonate chemistry, and will directly test the implications for a potentially sensitive life form, invertebrate larvae.</p>
<p>The objectives of this study are to measure the impact of key coastal habitats on natural pH variance, and to evaluate the implications these pH regimes have for developing invertebrate larvae. To achieve these objectives the investigators will characterize temporal and spatial carbonate chemistry variability inside and outside kelp forests in San Diego, California. With discrete water samples for the determination of total alkalinity and dissolved inorganic carbon, and continuous autonomous instruments which measure pH, dissolved oxygen, salinity, and temperature, a statistical characterization of carbonate chemistry variability will identify diurnal, seasonal and spatial trends as well as frequencies of maximum variation, rates of change, lowest potential pH (extreme statistics), and biologically-significant thresholds. Subsequently, prominent macrophyte-induced pH regimes will be mimicked in laboratory experiments and incorporated with ocean acidification predictions to test effects of (a) decreased pH, (b) varying pH about the mean, (c) changing variance about mean pH, and (c) pulsed exposure to extreme low pH, on larval survivorship, growth, and calcification responses of multiple species. Together, these laboratory and field studies will offer a mechanistic understanding of the effects of natural variance of carbonate chemistry in the context of ocean acidification for marine invertebrate larvae.</p>
<p>Four moorings identified as SeapHOx Moorings have been deployed in the San Diego La Jolla Kelp Forest in the vicinity of 32.8 N 117.3 W.</p>
<p><a href="http://bcodata.whoi.edu/SeapHOx/SeapHOx_mooring_locs.png" target="_blank">Mooring Locations</a></p>
SeapHOx
largerWorkCitation
project
eng; USA
oceans
9500 Gilman Drive La Jolla, California 92093-0218
2013-10-10
Coastal CA; San Diego La Jolla Kelp Forest; 32.8 N; 117.3 W
0
BCO-DMO catalogue of parameters from Urchin Fertilization Studies from Levin laboratory at Scripps Institute of Oceanography from 2009 to 2012 (SeapHOx project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/36658.rdf
Name: Lab_Id
Units: text
Description: <p>Lab Id</p>
http://lod.bco-dmo.org/id/dataset-parameter/36659.rdf
Name: Latitude
Units: decimal degrees
Description: <p>Approximate Latitude of the Lab; South is negative</p>
http://lod.bco-dmo.org/id/dataset-parameter/36660.rdf
Name: Longitude
Units: decimal degrees
Description: <p>Approximate Longitude of the Lab; West is negative</p>
http://lod.bco-dmo.org/id/dataset-parameter/36661.rdf
Name: Expt
Units: dimensionless
Description: <p>Experiment Number = 1-3</p>
http://lod.bco-dmo.org/id/dataset-parameter/36662.rdf
Name: Species
Units: text
Description: <p>Species = <i>S. Franciscanus</i> or <i>S. Purpuratus</i></p>
http://lod.bco-dmo.org/id/dataset-parameter/36663.rdf
Name: pH
Units: total scale
Description: <p>pH = pH of treatment (total scale; 15C)</p>
http://lod.bco-dmo.org/id/dataset-parameter/36664.rdf
Name: Sperm2Egg_Ratio
Units: ratio
Description: <p>Sperm:Egg = sperm-egg ratio used for treatment</p>
http://lod.bco-dmo.org/id/dataset-parameter/36665.rdf
Name: Sire_Identity
Units: dimensionless
Description: <p>Sire Identity = male identity (only used in Expt. 3)</p>
http://lod.bco-dmo.org/id/dataset-parameter/36666.rdf
Name: Rep
Units: dimensionless
Description: <p>Replicate Id</p>
http://lod.bco-dmo.org/id/dataset-parameter/36667.rdf
Name: Fertilization_Ratio
Units: ratio
Description: <p>Fertilization Ratio = ratio of fertilization eggs to total eggs</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
15460
https://datadocs.bco-dmo.org/file/M77Xqy6T53L2YX/Fertilization_Data.csv
Fertilization_Data.csv
Primary data file for dataset ID 4056
download
https://osprey.bco-dmo.org/dataset/4056/data/download
download
onLine
dataset
<p><strong>Sampling and Analytical Methodology:</strong><br />
Spawning was induced by injecting 0.55 M KCl through the peristomal membrane. Oocytes were collected in dishes of filtered seawater (FSW, 0.22 µm). Number of adults used in each experiment ranged from 2 – 5 females and 3 – 5 males. Sperm were collected dry from the gonopores of males and placed in a small vial and kept on ice until use. A 0.1% dilution from dry sperm was made to verify sperm motility, and then preserved in formalin to count sperm densities with a hemacytometer in order to calculate the amount of diluted sperm required to attain desired sperm-egg ratios and sperm densities for each treatment. Oocytes from multiple females were mixed and egg densities determined by counting 8 x 20 µl aliquots. Eggs were added to experimental beakers at a density of 5 eggs ml<sup>-1</sup>, and incubated in experimental conditions for 10 min before sperm were added. Dry sperm was diluted immediately before addition to experimental beakers. Following sperm addition the seawater in each replicate was gently stirred. Fertilization proceeded for 20 min and was arrested by transferring the contents of each replicate to a 1% formalin solution. The fertilization ratio, defined as the proportion of eggs fertilized, was determined by counting the frequency of occurrence of a fertilization envelope in at least 100 eggs per replicate. All experiments were carried out in the Scripps experimental aquarium facility during the spring of 2013. 500 ml glass beakers were used, and there were three replicates per treatment level.</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing Notes</strong><br />
Original file: "Fertilization_FINAL.txt" contributed by Christina Frieder<br />
- Approx Lat/Lon of Levin Lab appended to enable data discovery in MapServer<br />
- Parameter names edited to conform to BCO-DMO parameter naming conventions</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Hemocytometer
Hemocytometer
PI Supplied Instrument Name: Hemocytometer PI Supplied Instrument Description:Sperm were collected dry from the gonopores of males and placed in a small vial and kept on ice until use. A 0.1% dilution from dry sperm was made to verify sperm motility, and then preserved in formalin to count sperm densities with a hemacytometer in order to calculate the amount of diluted sperm required to attain desired sperm-egg ratios and sperm densities for each treatment. Instrument Name: Hemocytometer Instrument Short Name:Hemocytometer Instrument Description: A hemocytometer is a small glass chamber, resembling a thick microscope slide, used for determining the number of cells per unit volume of a suspension. Originally used for performing blood cell counts, a hemocytometer can be used to count a variety of cell types in the laboratory. Also spelled as "haemocytometer". Description from:
http://hlsweb.dmu.ac.uk/ahs/elearning/RITA/Haem1/Haem1.html.
Deployment: lab_SIO_Levin
lab_SIO_Levin
SIO Levin
SIO Levin
laboratory
lab_SIO_Levin
Lisa A. Levin
University of California-San Diego Scripps
http://levin.ucsd.edu/
Report describing lab_SIO_Levin
SIO Levin
SIO Levin
laboratory