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        <gco:CharacterString>VPR acquisition log sheets (pdf) Dataset Description: &amp;lt;p&amp;gt;Original hand-written log sheets for VPR casts recording initial conditions, date, time, location and observations.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;The Video Plankton Recorder is an underwater video microscope system designed to record images of plankton ranging in size from less than one half millimeter up to a few centimeters. A strobe light flashing at 20 times per second captures images at this rate. A program called AutoDeck reviews the images at about 15 frames per second and extracts Regions of Interest (ROIs) that may be plankton based on certain parameters such as brightness and sharpness (see Settings for ROI Extraction below).&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;amp;nbsp;&amp;lt;/p&amp;gt; Methods and Sampling: &amp;lt;p&amp;gt;The VPR&amp;amp;nbsp;was used in order to describe the abundance and vertical distribution of plankton taxa.&amp;amp;nbsp;The VPR was deployed off the aft via the same winch as the CTD and MOCNESS. After TI668, a stand was adapted which allowed the VPR to be strapped onto the Tioga CTD for simultaneous sampling. The hard-drive was removed following each cast and the data downloaded. Quick visual scrutiny of the data in Autodeck was performed ship-board to look for any depths of high pteropod abundance; no such depths were evident in the VPR data based on this preliminary examination.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;See the cruise reports for the recommended settings (Click on Deployment links, below, then Deployment Report URL.)&amp;lt;/p&amp;gt;</gco:CharacterString>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/55175.rdf" xlink:title="OCE-1316040" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1316040 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1316040</gmx:Anchor>
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In recognition of the need for basic research concerning the nature, extent and impact of ocean acidification on oceanic environments in the past, present and future, the goal of the SEES: OA program is to understand (a) the chemistry and physical chemistry of ocean acidification; (b) how ocean acidification interacts with processes at the organismal level; and (c) how the earth system history informs our understanding of the effects of ocean acidification on the present day and future ocean.
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NSF 10-530, FY 2010-FY2011
NSF 12-500, FY 2012
NSF 12-600, FY 2013
NSF 13-586, FY 2014
NSF 13-586 was the final solicitation that will be released for this program.
PI Meetings:
1st U.S. Ocean Acidification PI Meeting(March 22-24, 2011, Woods Hole, MA)
2nd U.S. Ocean Acidification PI Meeting(Sept. 18-20, 2013, Washington, DC)
3rd U.S. Ocean Acidification PI Meeting (June 9-11, 2015, Woods Hole, MA – Tentative)
NSF media releases for the Ocean Acidification Program:
Press Release 10-186 NSF Awards Grants to Study Effects of Ocean Acidification
Discovery Blue Mussels &quot;Hang On&quot; Along Rocky Shores: For How Long?
Discovery nsf.gov - National Science Foundation (NSF) Discoveries - Trouble in Paradise: Ocean Acidification This Way Comes - US National Science Foundation (NSF)
Press Release 12-179 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: Finding New Answers Through National Science Foundation Research Grants - US National Science Foundation (NSF)
Press Release 13-102 World Oceans Month Brings Mixed News for Oysters
Press Release 13-108 nsf.gov - National Science Foundation (NSF) News - Natural Underwater Springs Show How Coral Reefs Respond to Ocean Acidification - US National Science Foundation (NSF)
Press Release 13-148 Ocean acidification: Making new discoveries through National Science Foundation research grants
Press Release 13-148 - Video nsf.gov - News - Video - NSF Ocean Sciences Division Director David Conover answers questions about ocean acidification. - US National Science Foundation (NSF)
Press Release 14-010 nsf.gov - National Science Foundation (NSF) News - Palau's coral reefs surprisingly resistant to ocean acidification - US National Science Foundation (NSF)
Press Release 14-116 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: NSF awards $11.4 million in new grants to study effects on marine ecosystems - US National Science Foundation (NSF)</gco:CharacterString>
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&lt;p&gt;From NSF proposal abstract:&lt;/p&gt;
&lt;p&gt;Dissolution of excess anthropogenic CO2 into the ocean is causing the  marine environment to decrease in pH. This &quot;ocean acidification&quot; is  predicted to threaten a broad variety of marine organisms, particularly  calcifying animals such as the thecosome (i.e., shelled) pteropods.  These pelagic gastropods form an aragonite shell, are prey for a number  of commercially important fish, and are significant contributors to  carbon biogeochemistry. Their ecosystem importance, abundance, and  sensitivity to dissolution position them as an important group for  investigating the impacts of acidification. Our understanding of the  effect of high CO2 on pteropods and the pelagic ecosystem, however, is  limited primarily to short-term studies of adult calcification and  respiration response in the polar ecosystems. There have been no  seasonal studies of sensitivity and our understanding of the effect of  CO2 on pteropod early life stages is limited. Limacina retroversa is a  particularly abundant thecosome pteropod in the North Atlantic, where it  is prey for a number of fisheries species and other top predators. This  species is also the most common pteropod in the Gulf of Maine (GoM)  where it is present year round. L. retroversa thus offers the prospect  of a useful model pteropod species, given both its ecological importance  and its abundance in readily accessible waters. The investigators will  conduct a series of short cruises to sample L. retroversa on a seasonal  basis from local waters of the GoM near Cape Cod. The carbonate  chemistry of the GoM fluctuates seasonally, providing the  opportunity to assess the response of wild caught pteropods to natural  changes in CO2. By characterizing the carbonate chemistry of the water  column and measuring the metabolic rate, shell quality, and gene  expression of pteropods throughout the year, the researchers will  achieve a time series of pteropod sensitivity to CO2. Subsequently,  using experimental manipulations the investigators will explore the  effect of seasonal acclimation on pteropod response to short- and  medium-term exposure to enhanced CO2. Pteropods frequently lay eggs in  captivity, and at WHOI there is institutional expertise in maintaining  these individuals in the laboratory. Building on these strengths, the  researchers will also study the effect of CO2 on embryonic and larval  development in L. retroversa. These earliest life-stages of marine  calcifiers are thought to be especially sensitive since initial shell  precipitation and the highly energetic processes of growth and  development are impeded by CO2 exposure. They will also document  mortality, shell production, abnormality, and developmental rate of  clutches of pteropod embryos exposed to increased CO2.&lt;/p&gt;
&lt;p&gt;Intellectual  Merit: Thecosome pteropods are an abundant group of calcifying  zooplankters that have been chronically understudied, particularly in  temperate regions. Due to its accessibility and ecological importance,  L. retroversa can be developed as a valuable model, interesting both as  the dominant pteropod in the commercially-important GoM region and also  an abundant pteropod in the temperate waters of the North Atlantic. The  goal of this research is to augment our knowledge of the distribution of  L. retroversa, to attain an understanding of their seasonal sensitivity  to natural variability in CO2, and to see how this exposure impacts  responses to both short- and medium-term CO2 exposure. Using powerful  transcriptomic technologies, the research will transform our  understanding of this group by investigating the molecular mechanisms of  response in L. retroversa to both seasonality and varying durations and  intensities of acidification, contextualized by ecosystem- and  organism-level metrics. Furthermore the study will examine the  effect of CO2 on the eggs of pteropods for the first time, providing  insight into their sensitivity to an acidifying environment.&lt;/p&gt;</gco:CharacterString>
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                <gco:CharacterString>&amp;lt;p&amp;gt;The VPR&amp;amp;nbsp;was used in order to describe the abundance and vertical distribution of plankton taxa.&amp;amp;nbsp;The VPR was deployed off the aft via the same winch as the CTD and MOCNESS. After TI668, a stand was adapted which allowed the VPR to be strapped onto the Tioga CTD for simultaneous sampling. The hard-drive was removed following each cast and the data downloaded. Quick visual scrutiny of the data in Autodeck was performed ship-board to look for any depths of high pteropod abundance; no such depths were evident in the VPR data based on this preliminary examination.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;See the cruise reports for the recommended settings (Click on Deployment links, below, then Deployment Report URL.)&amp;lt;/p&amp;gt;</gco:CharacterString>
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                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/451.rdf" xlink:title="Video Plankton Recorder" xlink:actuate="onRequest">VPR</gmx:Anchor>
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            <gco:CharacterString>PI Supplied Instrument Name: VPR Instrument Name: Video Plankton Recorder Instrument Short Name:VPR   Instrument Description: The Video Plankton Recorder (VPR) is a video-microscope system used for imaging plankton and other particulate matter in the size range from a few micrometers to several centimeters. The VPR is essentially an underwater microscope. It consists of four video cameras (with magnifying optics) synchronized at 60 fields per second (fps) to a red-filtered 80 W xenon strobe (pulse duration = 1 microsecond). The current lens on each camera can be adjusted to provide a field of view between 5 mm and 10 cm. Use of higher magnification lenses is currently being explored for viewing protozoans (less than 1 micrometer resolution). The four cameras are set for concentric viewing fields so that a range of up to four magnifications can be viewed simultaneously, allowing a wide size range of plankton to be sampled. Depth of field is adjusted by the lens aperture setting, and the volume sampled in each video field ranges from about 1 ml to 1 liter, depending on lens settings. The cameras have been configured for stereoscopic viewing as well.A strobe on the other arm illuminates the imaged volume and flashes 60 times per second, producing 60 images per second of the particles and plankton in the water. The images are then saved internally on a computer hard disk and later plotted.

Deployment: Most commonly, the VPR is mounted in a frame and lowered into the water from the stern of the ship. Sometimes, a CTD also is mounted next to the VPR to collect depth, temperature, and salinity information at the same time as each video image. The instrument is lowered down through the water to a maximum depth of 350 meters to generate a profile of plankton/particle abundance and taxon group along with temperature and salinity. In addition to the towed configuration for mapping plankton distributions, it is possible to deploy the VPR in a fixed position (on a mooring) for viewing plankton swimming behaviors in two or three dimensions. The VPR instrument system has been used in both configurations, and deployment on ROVs has been proposed.

This definition was taken from the WHOI Ocean Instruments Web site  and from a US GLOBEC Newsletter.</gco:CharacterString>
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         <gmx:Anchor xlink:href="http://www.whoi.edu/main/ships/tioga" xlink:actuate="onRequest">R/V Tioga</gmx:Anchor>
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         <gmx:Anchor xlink:href="http://vocab.nerc.ac.uk/collection/C17/current/3313" xlink:actuate="onRequest">Community Standard Description</gmx:Anchor>
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              <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/authority/1.rdf" xlink:actuate="onRequest">International Council for the Exploration of the Sea</gmx:Anchor>
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                          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/person/51589.rdf" xlink:actuate="onRequest">Amy Maas</gmx:Anchor>
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                          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/affiliation/2.rdf" xlink:title="Affiliation" xlink:actuate="onRequest">Woods Hole Oceanographic Institution</gmx:Anchor>
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                                <gmd:URL>http://bcodata.whoi.edu/GoME_Pteropods/cruise_reports/Tioga715_Cruise_Report_final.pdf</gmd:URL>
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