Seawater and filter sample nitrogen and carbon isotopes from coastal and off-shore sites from multiple cruises in the coastal Washington, 2009-2012 (Regenerated Nitrogen project)

Website: https://www.bco-dmo.org/dataset/489045
Version: 2014-03-20

Project
» The Role of Regenerated Nitrogen for Rocky Shore Productivity (Regenerated Nitrogen)
ContributorsAffiliationRole
Pfister, CatherineUniversity of ChicagoPrincipal Investigator
Altabet, Mark A.University of Massachusetts Dartmouth (UMASSD-SMAST)Co-Principal Investigator
Post, DavidYale UniversityCo-Principal Investigator
Copley, NancyWoods Hole Oceanographic Institution (WHOI BCO-DMO)BCO-DMO Data Manager


Data Processing Description

See the other datasets associated with this project for methodology.

Seawater and filter sample isotope methodology is described in references cited below:
2014. Pather, S., C. A. Pfister, M. Altabet, D. M. Post. Ammonium cycling in the rocky intertidal: remineralization, removal and retention. Limnology and Oceanography 59:361-372. http://aslo.org/lo/toc/vol_59/issue_2/0361.html

in review (March 2014). Pfister, C. A., M. Altabet, D. Post.

Carbon system measurements are described in:
2012. Wootton, J. T. & C. A. Pfister. Carbon system measurements and potential climatic drivers at a site of rapidly declining ocean pH. PLoS ONE 7(12): e53396. doi:10.1371/journal.pone.0053396.  Data associated with this paper are uploaded to the World Ocean DataBase, www.nodc.noaa.gov

Related files and references:
2010. Pfister, C. A., F. Meyer, D. A. Antonopoulos. Metagenomic profiling of a microbial assemblage associated with the California mussel, Mytilus californianus: a node in networks of carbon and nitrogen cycling. PLoS ONE 5(5): e10518. doi:10.1371/ journal.pone.0010518. Metagenome data associated with this paper are uploaded to MGRAST server at metagenomics.anl.gov


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Data Files

File
N_regen_v3.csv
(Comma Separated Values (.csv), 205.35 KB)
MD5:2ca6c08a416b35a9efe78b8d99ca2c7a
Primary data file for dataset ID 489045

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Parameters

ParameterDescriptionUnits
yearyear yyyy
shore_offshoreclose to shore or off-shore text
cruise_idcruise identification unitless
month_localday of month 1-12
day_localday; local time 1-31
date_localdate; local time MM/DD/YYYY
time_locallocal time; Pacific time zone HH:MM:SS
yrday_localday and decimal time. eg. 326.5 for the 326th day of the year (November 22) at 1200 hours (noon). unitless
ISO_DateTime_LocalDate/Time (UTC) ISO formatted YYYY-MM-DDThh:mm:ss[.xx]Z
latitudelatitude; north is positive decimal degrees
longitudelongitude; east is positive decimal degrees
stationlocale of station text
sitesite code; may be same as station code text
depth_nomnominal depth meters
depthdepth meters
vol_filtvolume filtered liters
filterpacketfilter packet id unitless
del15Ntotal nitrogen isotopic composition (delta 15N:N14) parts per thousand vs. VSMOW (Vienna Standard Mean Ocean Water)
del13Ctotal carbon isotopic composition (delta C13: 13C/12C) parts per thousand vs. VSMOW (Vienna Standard Mean Ocean Water)
N_pctpercent total nitrogen percent
C_pctpercent total carbon percent
comment_filtercomments pertaining to the filter text
bottle_isoNnitrogen isotope bottle number integer
dN15_NO3nitrogen isotopic composition (delta 15N:N14) of nitrate parts per thousand vs. VSMOW (Vienna Standard Mean Ocean Water)
d18O_NO3oxygen isotopic composition (delta O18: 18O/16O) of nitrate parts per thousand vs. VSMOW (Vienna Standard Mean Ocean Water)
d15N_NH4nitrogen isotopic composition (delta 15N:N14) of ammonium parts per thousand vs. VSMOW (Vienna Standard Mean Ocean Water)
d15N_NO2nitrogen isotopic composition (delta 15N:N14) of nitrite parts per thousand vs. VSMOW (Vienna Standard Mean Ocean Water)
bottle_nutnutrient bottle number integer
PO4phosphate concentration microMolar
Sisilicate concentration microMolar
NO3nitrate concentration microMolar
NO2nitrite concentration microMolar
NH4ammonium concentration microMolar
sstempsea surface temperature??  This value is given for depths within a profile so it may actually be temperature, not sea surface temperature. degrees Celsius
salinitysalinity parts per thousand
pHpH: The measure of the acidity or basicity of an aqueous solution unitless; pH scale
O2_umol_kgdissolved oxygen concentration micromoles/kilogram
O2_ml_Ldissolved oxygen concentration milliliters/liter
O2_mg_Ldissolved oxygen concentration milligrams/liter
O2_sat_pcntdissolved oxygen concentration percent
chlchlorophyll-a concentration milligrams/liter
upwelling_daydaily average coastal upwelling index for 48 deg N; from http://www.pfeg.noaa.gov/products/pfel/modeled/indices/upwelling/upwelli... meters^3/second/100 meters cosastline
upwelling_monmonthly average coastal upwelling index for 48 deg N; from http://www.pfeg.noaa.gov/products/pfel/modeled/indices/upwelling/upwelli... meters^3/second/100 meters cosastline
condspecific conductance milliSiemens/centimeter
TDStotal dissolved solids grams/liter
LDOluminescent/optical dissolved oxygen milligrams/liter
LDO_pcentluminescent/optical dissolved oxygen saturation percent saturation
time_of_dayday = sample taken during day text
instrumentinstrument used for measurement text
d18O_vialoxygen isotopic composition (delta O18: 18O/16O) of seawater parts per thousand vs. VSMOW (Vienna Standard Mean Ocean Water)
d13C_seawatercarbon isotopic composition (delta C13: 13C/12C) of seawater parts per thousand vs. VSMOW (Vienna Standard Mean Ocean Water)
bottle_dicksonDickson bottle number integer
salsalinity unitless
TALKtotal alkalinity micromoles/kilogram
DICdissolved inorganic carbon concentration micromoles/kilogram
bottle_niskinNiskin bottle number integer
commentcomments text

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Deployments

CB946

Website
Platform
R/V Clifford A. Barnes
Start Date
2010-07-21
End Date
2010-07-23
Description
Methods & Sampling
Cruise track from http://www.rvdata.us/catalog/Clifford_A._Barnes

CB948

Website
Platform
R/V Clifford A. Barnes
Start Date
2010-08-25
End Date
2010-08-27
Description
Methods & Sampling
cruise track from http://www.rvdata.us/catalog/Clifford_A._Barnes.

CB958

Website
Platform
R/V Clifford A. Barnes
Start Date
2011-06-21
End Date
2011-06-23
Description
Methods & Sampling
Cruise track from http://www.rvdata.us/catalog/Clifford_A._Barnes.

CB961

Website
Platform
R/V Clifford A. Barnes
Start Date
2011-07-25
End Date
2011-07-27
Description
Methods & Sampling
Cruise track from http://www.rvdata.us/catalog/Clifford_A._Barnes.

CB963

Website
Platform
R/V Clifford A. Barnes
Start Date
2011-08-16
End Date
2011-08-18
Description
Methods & Sampling
Cruise track from http://www.rvdata.us/catalog/Clifford_A._Barnes.

CB978

Website
Platform
R/V Clifford A. Barnes
Start Date
2012-06-29
End Date
2012-06-30
Description
Methods & Sampling
Cruise track from http://www.rvdata.us/catalog/Clifford_A._Barnes.

CB981

Website
Platform
R/V Clifford A. Barnes
Start Date
2012-07-26
End Date
2012-07-27
Description
Methods & Sampling
Cruise track from http://www.rvdata.us/catalog/Clifford_A._Barnes.

CB983

Website
Platform
R/V Clifford A. Barnes
Start Date
2012-08-14
End Date
2012-08-16
Description
Methods & Sampling
Cruise track from http://www.rvdata.us/catalog/Clifford_A._Barnes.

CB943

Website
Platform
R/V Clifford A. Barnes
Start Date
2010-06-22
End Date
2010-06-24

Pfister_2009

Website
Platform
Pfister small boat
Start Date
2009-06-24
End Date
2009-06-24
Description
Seawater sampling in a small boat.

Pfister_shore

Website
Platform
Pfister shore
Start Date
2009-06-24
End Date
2012-08-20


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Project Information

The Role of Regenerated Nitrogen for Rocky Shore Productivity (Regenerated Nitrogen)


Coverage: coastal northeast Pacific Ocean


NSF Award Abstract:
A fundamental and persistent question in a multitude of ecosystems is the extent to which new versus regenerated nutrients support ecosystem productivity. In coastal marine systems, nitrate derived from upwelling (= new nitrogen) and ammonium regeneration in coastal waters and sediments (= regenerated nitrogen) are major nitrogen sources that fuel coastal ocean productivity. Because inorganic nitrogen availability clearly regulates production in a large number of areas, understanding nitrogen supply is essential. In open coast regions away from river mouths, nitrate inputs are determined by large-scale physical processes promoting upwelling of deep, nutrient-rich water including wind direction and intensity. In contrast, regenerated nitrogen (mainly ammonium) is generally the result of local animal and microbial processes. Along marine rocky shores, where upwelling is typically used as a proxy for productivity, we know very little about the dynamics of regenerated nutrients and their potential contribution to productivity at larger scales; only upwelling is typically used as a proxy for productivity. Associations of the abundant California mussel, Mytilus californianus, with water nutrients, algal productivity, stable isotope signatures, and microbial genetics indicate potentially strong regeneration of nitrogen by these animals and suggest an important secondary role of nitrifying microbes affiliated with these animals.

In this project, the investigators will quantify the relative contribution of regenerated nitrogen on rocky shores through censuses and experiments across a gradient of mussel abundance. They will use stable nitrogen and oxygen isotopes of ammonium, nitrite, and nitrate to disentangle the contribution of different biological processes versus upwelling to the nitrogen supply and uptake of rocky shore regions. This includes both natural abundance and tracer addition studies.

Broader Impacts. Regenerated nitrogen supply, as opposed to new nitrogen via upwelling, is a local process dependent upon an intact animal community. However, mussels and other nearshore animals may be particularly vulnerable to a changing thermal environment, toxic algal blooms, and ocean acidification. Given the dramatic changes to the coastal nitrogen cycle in recent years, and potential changes to currents, upwelling, ocean chemistry, and El Niño frequencies portended by global changes to our climate, we to know the relative effect of local versus larger scale oceanic events on the nitrogen cycle. The proposed work links biological interactions in situ with its implications for coastal productivity.

In addition to expected publications in high quality journals, educational activities will continue to focus on graduate and undergraduate education and mentoring. The proposal will fund two graduate students and two undergraduates per year. The PI's will work closely with government (Olympic Marine National Sanctuary) and tribal (Makah Tribe) representatives to communicate this research. We will also work with Makah Museum Board of Trustees and the Makah Higher Education Committee to identify Makah students as research assistants. All three PI's teach broadly across their respective campuses, instructing almost every type of undergraduate major.



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Funding

Funding SourceAward
NSF Division of Ocean Sciences (NSF OCE)

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