<div><p>Total microzooplankton abundance and community composition was determined for samples preserved with 10% acid Lugol’s solution (final concentration) and stored in the dark at room temperature until analysis (Throndsen, 1978). One hundred mL samples were settled in Utermohl chambers for at least 18 h and enumerated using light microscopy at 200x magnification. Microzooplankton were identified to the genus level. The use of acid Lugol’s solution obscured Chl <em>a</em> fluorescence and made it impossible to distinguish phototrophic from heterotrophic dinoflagellates based on autofluorescence. However, certain heterotrophic dinoflagellates such as <em>Protoperidinium </em>and <em>Gyrodinium</em> could be identified based on morphology, and were thus included in the counts.</p>
<p><strong>References</strong></p>
<p><em><em>Throndsen, J. 1978. Preservation and storage. In Phytoplankton manual, ed. A. Sournia, 69-74. Paris: UNESCO.</em></em></p>
<p><em><em>Utermohl, H. 1958. Zur Vervollkommung der quantitativen phytoplankton-methodik. Mitteilungen der Internationalen Vereinigung fur Limnologie 9: 1-38.</em></em></p></div>
Microzooplankton abundance from temp and iron experiments on Antarctic phyto and microzooplankton
<div><p><strong>Experimental Design</strong></p>
<p>Experiments were conducted during the CORSACS (Controls On Ross Sea Algal Community Structure) expedition in January 2006 to the Ross Sea, Antarctica, onboard the RVIB Nathaniel B. Palmer (cruise NBP-0601). Water was collected at 75.00S, 177.36E using a trace metal clean towed-intake surface water Teflon diaphragm pumping system (Bruland et al., 2005). Water was prescreened through acid-washed 200μm Nitex mesh to eliminate large zooplankton and collected into a 50-L mixing carboy. Collected water was gently mixed and dispensed into 12 4.5-L and 12 2.7-L acid washed trace metal clean clear polycarbonate bottles for incubation. Half of the bottles were spiked with 1.0nM FeCl3 (final concentration) at the beginning of the experiment. Bottles were incubated in two temperature controlled deck-board incubators (Feng et al., 2009; Hare et al., 2007). Incubators were screened to 18% of Io using two layers of neutral density filter. One incubator was kept at ambient temperature (0 deg C), while the temperature in the other was gradually increased to 4 deg C over the course of 24 h. Bottles were incubated for seven days. The 4.5-L bottles were sampled daily and the 2.7-L bottles were only sampled on the final day of the experiment. All sampling occurred under a laminar flow hood using trace metal clean techniques.</p>
<p><strong>References</strong></p>
<p><em><em>Bruland, K.W., E.L. Rue, G.J. Smith, and G.R. DiTullio. 2005. Iron, macronutrients and diatom blooms in the Peru upwelling regime: brown and blue waters of Peru. Marine Chemistry 93: 81-103.</em></em></p>
<p><em><em>Feng, Y., C.E. Hare, K. Leblanc, G.R. DiTullio, P.A. Lee, S.W. Wilhelm, J. Sun, J.M. Rose, N. Nemcek, I. Benner, and D.A. Hutchins. 2009. The effects of increased pCO2 and temperature on the North Atlantic Spring Bloom: I. The phytoplankton community and biogeochemical response. Marine Ecology Progress Series 388: 13-25.</em></em></p>
<p><em><em>Hare, C.E., K. Leblanc, G.R. DiTullio, R.M. Kudela, Y. Zhang, P.A. Lee, S.F. Riseman, and D.A. Hutchins. 2007. Consequences of increased temperature and CO2 for phytoplankton community structure in the Bering Sea. Marine Ecology Progress Series 352: 9-16.</em></em></p></div>
microzoo_abundance_Ant1
<div><p><strong>BCO-DMO Processing Notes:</strong></p>
<p>- File was sorted by treatment<br />
- Added lat,lon values of original water sampling location to file<br />
- Added BCO-DMO header line<br />
- File was transposed to serve data by taxon and abundance in columns</p></div>
491074
microzoo_abundance_Ant1
2014-02-10T13:52:18-05:00
2014-02-10T13:52:18-05:00
2023-07-07T16:10:26-04:00
urn:bcodmo:dataset:491074
Microzooplankton abundance from phyto and microzooplankton experiments from the RVIB Nathaniel B. Palmer NBP0601 cruise in the Ross Sea, Southern Ocean from 2005-2006 (CORSACS project, Antarctic microzooplankton project)
false
Rose, J., Hutchins, D. A. (2014) Microzooplankton abundance from phyto and microzooplankton experiments from the RVIB Nathaniel B. Palmer NBP0601 cruise in the Ross Sea, Southern Ocean from 2005-2006 (CORSACS project, Antarctic microzooplankton project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2014-02-06) Version Date 2014-02-06 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/491074 [access date]
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