http://lod.bco-dmo.org/id/dataset/516969
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2014-06-17
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Coral physiology, algal density and type collected from the Reef Field Sites from the Puerto Morelos, Mexico & Kaneohe Bay, Hawaii in 2009 (repeat coral bleaching project)
2015-02-13
publication
2015-02-13
revision
BCO-DMO Linked Data URI
2015-02-13
creation
http://lod.bco-dmo.org/id/dataset/516969
Andréa G. Grottoli
Ohio State University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Grottoli, A. G. (2015) Coral physiology, algal density and type collected from the Reef Field Sites from the Puerto Morelos, Mexico & Kaneohe Bay, Hawaii in 2009 (repeat coral bleaching project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2015-02-13) Version Date 2015-02-13 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/516969 [access date]
Coral metabolism, energy reserves, calcification, algal density and type Dataset Description: <p>Coral metabolism, energy reserves, calcification, algal density and type.</p> Methods and Sampling: <p>Full details of the experimental design are in:<br />
Grottoli AG, Warner ME, Levas SJ, Aschaffenburg MD, Schopef B, McGinley M, Baumann J, Matsui Y. 2014. Cumulative impact of annual coral bleaching can turn some coral species winners into losers. <em>Global Change Biology</em>.&nbsp; doi:<a href="http://dx.doi.org/10.1111/gcb.12658" target="_blank">10.1111/gcb.12658</a></p>
<p>A brief description of the analytical methods follows.</p>
<p><strong><em>Calcification, endosymbiotic algae concentration, total energy reserves, and Symbiodinium identification</em></strong>.&nbsp; Calcification rates were calculated from the buoyant weight data (Jokiel<em> et al.</em>, 1978) and standardized to surface area.&nbsp; Endosymbiont cell concentration (Warner<em> et al.</em>, 2006), and total soluble lipid, soluble animal protein, and soluble animal carbohydrates (Levas<em> et al.</em>, 2013, Rodrigues &amp;&nbsp; Grottoli, 2007) were measured on all frozen fragments. Total energy reserves were calculated as the sum of total lipids, protein, and carbohydrates and reported in Joules (Gnaiger &amp;&nbsp; Bitterlich, 1984) per gram ash free dry weight of coral tissue. Since polyp structure and the coral tissue thickness of each species are different, this normalization facilitates inter-species comparisons (Edmunds &amp;&nbsp; Gates, 2002). Genetic characterizations of <em>Symbiodinium</em> were determined by amplification of the internal-transcribed spacer 2 region (ITS2), followed by denaturing gradient gel electrophoresis and cycle-sequencing (Warner<em> et al.</em>, 2006). This method reliably identifies the dominant symbiont type in healthy and bleached corals (LaJeunesse<em> et al.</em>, 2004, LaJeunesse<em> et al.</em>, 2009, Warner<em> et al.</em>, 2006) and provides qualitative identification of other background <em>Symbiodinium</em>, either within different clades (e.g., endosymbionts A3 vs B1) or at the intra-cladal scale (e.g., endosymbionts A3 and A13) within the same coral fragment.&nbsp; The dominant ITS2-types (intra-cladal designations) are listed for each coral species in the text, while for statistical analyses (described below), the dominant symbiont for each coral fragment treatment<sup>-1</sup> was grouped by clade. Specific quantitative PCR for all clades confirmed the accuracy of scoring dominant bands by DGGE analysis (data not shown, McGinley, 2012).</p>
<p><strong><em>Photosynthesis, respiration, and feeding</em>.</strong>&nbsp; Maximal photosynthesis and respiration rates were measured via changes in dissolved oxygen on each individual coral fragment immediately following their respective thermal stress then standardized to ash free dry weight (Rodrigues &amp;&nbsp; Grottoli, 2007). All fragments were placed back on the reef and then feeding rates of each coral fragment were determined using methods in Palardy et al. (2008). Photosynthesis and respiration were used to calculate the percent Contribution of Zooxanthellae (<em>Symbiodinium </em>spp.) to Animal Respiration (CZAR) (Muscatine<em> et al.</em>, 1981), while respiration and feeding rates were used to calculate the percent Contribution of Heterotrophy to Animal Respiration CHAR (Grottoli<em> et al.</em>, 2006, Palardy <em>et al.</em>, 2008). The Contribution of the Total acquired fixed carbon relative to Animal Respiration (CTAR) was calculated as the sum of CZAR and CHAR.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0825413 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0825413
completed
Andréa G. Grottoli
Ohio State University
614-292-5782
School of Earth Sciences 125 South Oval Mall
Columbus
OH
43210
United States
grottoli.1@osu.edu
pointOfContact
asNeeded
Dataset Version: 2015-02-13
Unknown
lat
lon
species
treatment_year
status
treatment_recovery_time
genotype
CZAR
CHAR
CTAR
energy_rsrv_total
calcification
count_endosymbiont
symbiodinium_primary_type
symbiodinium_secondary_type
symbiodinium_tertiary_type
theme
None, User defined
latitude
longitude
species
treatment
sample type
No BCO-DMO term
count
featureType
BCO-DMO Standard Parameters
Coral_physiology_field_exper
service
Deployment Activity
Puerto Morelos, Mexico & Kaneohe Bay, Hawaii
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Physiology and Biogeochemistry of Repeatedly Bleached and Recovering Caribbean Corals
https://osprey.bco-dmo.org/project/516103
Physiology and Biogeochemistry of Repeatedly Bleached and Recovering Caribbean Corals
<p><em>(Extracted from the NSF award abstract)</em></p>
<p>The overall stability and health of coral reefs is declining world-wide at an unprecedented rate. Mass coral bleaching, wherein exposure to elevated temperature leads to the loss of significant numbers of endosymbiotic dinoflagellates (Symbiodinium spp., commonly called zooxanthellae) and/or photosynthetic pigments, serves as a primary global example of how fragile this symbiosis is. While we have begun to understand the ecological and physiological impacts of bleaching, there remain key fundamental gaps in knowledge. In particular, it is becoming increasingly clear that a) not all corals either respond to, or recover from, bleaching events the same way, and that b) the impact of annual or repeated bleaching events on corals has not been examined in sufficient detail. Several non-mutually exclusive ecological and physiological pathways could impact how a particular coral species succumbs to or recovers from bleaching. Recent evidence suggests that the following features may play key roles for coral survival in the face of future seawater warming and mass bleaching events: 1) shifts in trophic partitioning (e.g., proportional reliance on autotrophy and heterotrophy) and energy reserve utilization, 2) enhanced thermal tolerance through host and algal-mediated physiological responses, and 3) harboring of different Symbiodinium phylotypes. However, these mechanisms have yet to be investigated in a unified approach that covers the entire coral holobiont system (algae, host tissue, and skeleton), or under scenarios of repeated bleaching.</p>
<p>The overall objectives of this study are as follows: 1) to determine the effect of single and repeated bleaching on the physiology, biogeochemistry, and recovery of some Caribbean coral species, and 2) to determine which Symbiodinium-type and host-species combinations are more resilient to single and repeated bleaching, what aspects of their physiology and biogeochemistry render them resilient, and to use this information to evaluate the long-term persistence of Caribbean coral reefs. To address these objectives, the following physiological variables will be measured: 1) Symbiodinium type, photochemical function and algal stress physiology, and 2) animal host energy reserves, defense enzyme concentration, skeletal growth, and feeding capacity in the corals Porites porites, Porites astreoides, and Montastraea faveolata. Corals will be examined immediately following thermal stress designed to approximate natural bleaching, and recovery will be monitored over short and long-term time scales. Next, the impact of repeated bleaching will be examined in the subsequent year, followed by examination over the next recovery period. This research is designed to simultaneously evaluate the symbiotic algae, coral host, and skeleton, and to identify patterns of physiological responses and recovery of each Symbiodinium-type and host-species combination that would be indicative of the resilience capacity of Caribbean corals to future more frequent thermal perturbations.</p>
repeat coral bleaching
largerWorkCitation
project
eng; USA
biota
oceans
Puerto Morelos, Mexico & Kaneohe Bay, Hawaii
2015-02-13
Puerto Morelos, Mexico
0
BCO-DMO catalogue of parameters from Coral physiology, algal density and type collected from the Reef Field Sites from the Puerto Morelos, Mexico & Kaneohe Bay, Hawaii in 2009 (repeat coral bleaching project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/517270.rdf
Name: lat
Units: decimal degrees
Description: <p>Latitude component of geographic position where experiments were conducted.</p>
http://lod.bco-dmo.org/id/dataset-parameter/517271.rdf
Name: lon
Units: decimal degrees
Description: <p>Longitude component of geographic position where experiments were conducted.</p>
http://lod.bco-dmo.org/id/dataset-parameter/517274.rdf
Name: species
Units: dimensionless
Description: <p>Coral species sampled for use in experiment.</p>
http://lod.bco-dmo.org/id/dataset-parameter/517285.rdf
Name: treatment_year
Units: dimensionless
Description: <p>Consecutive bleaching treatments applied to coral samples, where 1 = single bleaching treatment or control conditions in the tanks for 15 days followed by up to a year on the reef, and 2 = repeat bleaching treatment and control conditions for 17 days in the tanks followed by up to a year on the reef. .</p>
http://lod.bco-dmo.org/id/dataset-parameter/517286.rdf
Name: status
Units: dimensionless
Description: <p>Status represents treatment conditions applied to coral samples during experiment, where 1=control and 2=treatment conditions.</p>
http://lod.bco-dmo.org/id/dataset-parameter/517287.rdf
Name: treatment_recovery_time
Units: dimensionless
Description: <p>Number of years of applied treatment conditions and observation of recovery time on the reef, where:<br />
0 = Year 1, 0 month on the reef<br />
1.5 = Year 1, 6 weeks on the reef<br />
13 = Year 2, 0 months on the reef<br />
14.5 = Year 2, 6 weeks on the reef</p>
http://lod.bco-dmo.org/id/dataset-parameter/517288.rdf
Name: genotype
Units: dimensionless
Description: <p>Genotype number represents each parent colony in the study from which all sample fragments were collected, where numbers 1-9 = each parent colony per species used in the experiment.</p>
http://lod.bco-dmo.org/id/dataset-parameter/517289.rdf
Name: CZAR
Units: percent
Description: <p>Contribution of Zooxanthellae (Symbiodinium spp.) to Animal Respiration sensu Muscatine et al., (1981).</p>
http://lod.bco-dmo.org/id/dataset-parameter/517330.rdf
Name: CHAR
Units: percent
Description: <p>Contribution of Heterotrophy to Animal Respiration sensu Grottoli et al., (2006) and Palardy et al., (2008).</p>
http://lod.bco-dmo.org/id/dataset-parameter/517331.rdf
Name: CTAR
Units: percent
Description: <p>Contribution of the Total acquired fixed carbon relative to Animal Respiration (CZAR + CHAR).</p>
http://lod.bco-dmo.org/id/dataset-parameter/517397.rdf
Name: energy_rsrv_total
Units: joules per gram dry weight
Description: <p>The total coral soluble lipids + animal soluble protein + animal soluble carbohydrates.</p>
http://lod.bco-dmo.org/id/dataset-parameter/517398.rdf
Name: calcification
Units: milligrams per cm^2 per day
Description: <p>Calification rate.</p>
http://lod.bco-dmo.org/id/dataset-parameter/517399.rdf
Name: count_endosymbiont
Units: cells per cm^2
Description: <p>Number of endosymbiont cells unit area.</p>
http://lod.bco-dmo.org/id/dataset-parameter/517400.rdf
Name: symbiodinium_primary_type
Units: dimensionless
Description: <p>Dominant endosymbiont type.</p>
http://lod.bco-dmo.org/id/dataset-parameter/517401.rdf
Name: symbiodinium_secondary_type
Units: dimensionless
Description: <p>Secondary endosymbiont type.</p>
http://lod.bco-dmo.org/id/dataset-parameter/517402.rdf
Name: symbiodinium_tertiary_type
Units: dimensionless
Description: <p>Tertiary endosymbiont type.</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
19639
https://datadocs.bco-dmo.org/file/k66gxA1Ck459Km/coral_physiology.csv
coral_physiology.csv
Primary data file for dataset ID 516969
download
https://osprey.bco-dmo.org/dataset/516969/data/download
download
onLine
dataset
<p>Full details of the experimental design are in:<br />
Grottoli AG, Warner ME, Levas SJ, Aschaffenburg MD, Schopef B, McGinley M, Baumann J, Matsui Y. 2014. Cumulative impact of annual coral bleaching can turn some coral species winners into losers. <em>Global Change Biology</em>.&nbsp; doi:<a href="http://dx.doi.org/10.1111/gcb.12658" target="_blank">10.1111/gcb.12658</a></p>
<p>A brief description of the analytical methods follows.</p>
<p><strong><em>Calcification, endosymbiotic algae concentration, total energy reserves, and Symbiodinium identification</em></strong>.&nbsp; Calcification rates were calculated from the buoyant weight data (Jokiel<em> et al.</em>, 1978) and standardized to surface area.&nbsp; Endosymbiont cell concentration (Warner<em> et al.</em>, 2006), and total soluble lipid, soluble animal protein, and soluble animal carbohydrates (Levas<em> et al.</em>, 2013, Rodrigues &amp;&nbsp; Grottoli, 2007) were measured on all frozen fragments. Total energy reserves were calculated as the sum of total lipids, protein, and carbohydrates and reported in Joules (Gnaiger &amp;&nbsp; Bitterlich, 1984) per gram ash free dry weight of coral tissue. Since polyp structure and the coral tissue thickness of each species are different, this normalization facilitates inter-species comparisons (Edmunds &amp;&nbsp; Gates, 2002). Genetic characterizations of <em>Symbiodinium</em> were determined by amplification of the internal-transcribed spacer 2 region (ITS2), followed by denaturing gradient gel electrophoresis and cycle-sequencing (Warner<em> et al.</em>, 2006). This method reliably identifies the dominant symbiont type in healthy and bleached corals (LaJeunesse<em> et al.</em>, 2004, LaJeunesse<em> et al.</em>, 2009, Warner<em> et al.</em>, 2006) and provides qualitative identification of other background <em>Symbiodinium</em>, either within different clades (e.g., endosymbionts A3 vs B1) or at the intra-cladal scale (e.g., endosymbionts A3 and A13) within the same coral fragment.&nbsp; The dominant ITS2-types (intra-cladal designations) are listed for each coral species in the text, while for statistical analyses (described below), the dominant symbiont for each coral fragment treatment<sup>-1</sup> was grouped by clade. Specific quantitative PCR for all clades confirmed the accuracy of scoring dominant bands by DGGE analysis (data not shown, McGinley, 2012).</p>
<p><strong><em>Photosynthesis, respiration, and feeding</em>.</strong>&nbsp; Maximal photosynthesis and respiration rates were measured via changes in dissolved oxygen on each individual coral fragment immediately following their respective thermal stress then standardized to ash free dry weight (Rodrigues &amp;&nbsp; Grottoli, 2007). All fragments were placed back on the reef and then feeding rates of each coral fragment were determined using methods in Palardy et al. (2008). Photosynthesis and respiration were used to calculate the percent Contribution of Zooxanthellae (<em>Symbiodinium </em>spp.) to Animal Respiration (CZAR) (Muscatine<em> et al.</em>, 1981), while respiration and feeding rates were used to calculate the percent Contribution of Heterotrophy to Animal Respiration CHAR (Grottoli<em> et al.</em>, 2006, Palardy <em>et al.</em>, 2008). The Contribution of the Total acquired fixed carbon relative to Animal Respiration (CTAR) was calculated as the sum of CZAR and CHAR.</p>
Specified by the Principal Investigator(s)
<p>Three statistical outliers were removed in order to meet assumptions of normality and homogeneity prior to ANOVA analyses.&nbsp; These data are flagged in the excel file and the removed value is included as a comment.&nbsp; Details of the statistical analysis methods are in Grottoli<em> et al.&nbsp;</em>(in press).</p>
<p>BCO-DMO Data Processing:<br />
- Added BCO-DMO header info to include brf dscrptn, PI and version<br />
- Edited parameter headers to comply with BCO-DMO convention:</p>
<ul>
<li>Total EnRes&nbsp;--&gt;&nbsp;energy_rsrv_total</li>
<li>'number endosymbiont cells/cm2' --&gt; count_endosymbiont</li>
<li>1<sup>o</sup> Symbiodinium type --&gt; symbiodinium_primary_type</li>
<li>2<sup>o</sup> Symbiodinium type --&gt; symbiodinium_secondary_type</li>
<li>3<sup>o</sup> Symbiodinium type --&gt; symbiodinium_tertiary_type</li>
<li>year&nbsp;--&gt;&nbsp;treatment_year</li>
<li>time --&gt; treatment_recovery_time</li>
</ul>
<p>- Species was decoded and genus species names were included in data, replacing&nbsp;codes of 1,2,3<br />
- Replaced all "." with "nd"<br />
- Added lat/lon of the collection site of coral fragments (Puerto Morelos 20.8333, 86.8666)<br />
- All precisions were edited to two decimals after consultation with PI.<br />
- Corrected longitude values to negative degrees. (were positive); 13 Feb 2015.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Deployment: Coral_physiology_field_exper
Coral_physiology_field_exper
Reef Field Sites
laboratory
Coral_physiology_field_exper
Andréa G. Grottoli
Ohio State University
Reef Field Sites
laboratory