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Water samples were collected from CTD Niskin bottles. Water samples for HPLC analyses were taken from one Niskin bottle per cast-depth combination included in the dataset. Note that the niskin_sampled column indicates which Niskin bottle the HPLC sample was taken from. The niskins_fired column indicates all Niskin bottle numbers fired at the specified depth, though the HPLC sample was taken from only one of those bottles.<\/p>\n
Chlorophyll and accessory pigment composition was analyzed by high performance liquid chromatography (HPLC; Agilent 1100). Culture aliquots were filtered on Whatmann GF\/F filters, flash frozen in liquid nitrogen, and stored at -80\u00b0C until analysis. Just prior to analysis, pigments were extracted overnight in acetone at -20\u00b0C. The following day extracted pigments were centrifuged and measured using a gradient elution method (DiTullio and Geesey, 2003), a modification of the Zapata et al 2000 method. Chromatographic separation was performed using a Waters C8 symmetry column, photodiode array and fluorescence detectors. The internal standard, \u03b2-Apo-8-carotenal-trans standard (Fluka Chemical Corp., USA) was added to extracted pigments as a peak reference. Individual pigment peaks were quantified with Chemstation software (revision B.03.01, Agilent) and our pigment action spectra library calibrated using pigment standards from DHI LABS (Hoersholm, Denmark) and in-house purifications of non-commercially available pigments. Coefficient of variation among replicate HPLC injections is < 3% and our limit of detection is approximately 1 ng L-1.<\/p><\/div>","@type":"rdf:HTML"}],"http:\/\/ocean-data.org\/schema\/hasBriefDescription":[{"@value":"HPLC pigment analyses of CTD collected samples on KN207-03 cruise (NA-VICE).","@language":"en-US"}],"http:\/\/purl.org\/dc\/terms\/description":[{"@value":"
Dataset includes HPLC pigment analyses of CTD collected samples from the KN207-03 cruise (Northeast Atlantic Ocean transect from Azores to Iceland).<\/p><\/div>","@type":"rdf:HTML"}],"http:\/\/www.w3.org\/2000\/01\/rdf-schema#label":[{"@value":"HPLC Pigments","@type":"xsd:string"}],"http:\/\/ocean-data.org\/schema\/hasProcessingDescription":[{"@value":"
Response factors for pigments were performed using dilutions of calibration standards. Full details of data processing and methods used can be found in: BCO-DMO Processing Notes:
\nDiTullio, G. R. & Geesey, M. E. (2002) Photosynthetic pigments in marine algae and bacteria. In:<\/em> BITTON, G. (ed.) The Encyclopedia of Environmental Microbiology.<\/em> New York: John Wiley & Sons Inc.<\/p>\n
\n- Modified parameter names to conform to BCO-DMO conventions;
\n- Blanks (missing data) replaced with \"nd\" meaning \"no data\";
\n- Separated the original \"niskin\" column into two columns: \"niskin_sampled\" and \"niskins_fired\";
\n- Converted original longitude values provided as positive degrees West to negative degrees East;
\n- Added ISO_DateTime_UTC column from original date_gmt and time_gmt fields;
\n- Transposed data to convert columns containing pigment concentrations into rows.<\/p><\/div>","@type":"rdf:HTML"}],"http:\/\/purl.org\/dc\/terms\/identifier":[{"@value":"517634","@type":"xsd:int"}],"http:\/\/purl.org\/dc\/terms\/title":[{"@value":"HPLC Pigments"}],"http:\/\/purl.org\/dc\/terms\/date":[{"@value":"2014-06-26T11:57:25-04:00","@type":"xsd:dateTime"}],"http:\/\/purl.org\/dc\/terms\/created":[{"@value":"2014-06-26T11:57:25-04:00","@type":"xsd:dateTime"}],"http:\/\/purl.org\/dc\/terms\/modified":[{"@value":"2023-07-07T16:10:26-04:00","@type":"xsd:dateTime"}],"http:\/\/rdfs.org\/ns\/void#inDataset":[{"@id":"http:\/\/www.bco-dmo.org\/"}],"http:\/\/ocean-data.org\/schema\/namedGraph":[{"@value":"urn:bcodmo:dataset:517634","@type":"xsd:token"}],"http:\/\/ocean-data.org\/schema\/osprey_page":[{"@id":"https:\/\/www.bco-dmo.org\/dataset\/517634"}],"http:\/\/ocean-data.org\/schema\/identifier":[{"@value":"_:Identifier517634"}],"http:\/\/ocean-data.org\/schema\/datasetTitle":[{"@value":"HPLC pigment analyses of CTD-collected samples from R\/V Knorr cruise KN207-03 in the North Atlantic (transect from Ponta Delgada, Azores to Reykjavik, Iceland) in 2012 (NA-VICE project)","@language":"en-US"}],"http:\/\/ocean-data.org\/schema\/abstract":[{"@value":"Dataset includes HPLC pigment analyses of CTD collected samples from the KN207-03 cruise (Northeast Atlantic Ocean transect from Azores to Iceland).","@language":"en-US"}],"http:\/\/purl.org\/dc\/terms\/rights":[{"@id":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"http:\/\/ocean-data.org\/schema\/deprecated":[{"@value":"false","@type":"xsd:boolean"}],"http:\/\/ocean-data.org\/schema\/temporalExtent":[{"@value":"_:temporalExtent517634"}],"http:\/\/ocean-data.org\/schema\/spatialCoverage":[{"@value":"_:spatialCoverage517634"}],"http:\/\/purl.org\/dc\/terms\/bibliographicCitation":[{"@value":"DiTullio, G. (2014) HPLC pigment analyses of CTD-collected samples from R\/V Knorr cruise KN207-03 in the North Atlantic (transect from Ponta Delgada, Azores to Reykjavik, Iceland) in 2012 (NA-VICE project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). 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