http://lod.bco-dmo.org/id/dataset/521312
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2014-08-01
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Experimental results: cellular particulate DMSP from E. huxleyi at 3 pCO2 levels, 2011-2012 (E Hux Response to pCO2 project)
2014-07-03
publication
2014-07-03
revision
BCO-DMO Linked Data URI
2014-07-03
creation
http://lod.bco-dmo.org/id/dataset/521312
M. Brady Olson
Western Washington University - Shannon Point Marine Center
principalInvestigator
Brooke Love
Western Washington University
principalInvestigator
Suzanne Strom
Western Washington University - Shannon Point Marine Center
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Olson, M. Brady, Strom, S., Love, B. (2014) Experimental results: cellular particulate DMSP from E. huxleyi at 3 pCO2 levels, 2011-2012 (E Hux Response to pCO2 project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2014-07-03) Version Date 2014-07-03 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/521312 [access date]
Cellular particulate DMSP from E. huxleyi at 3 pCO2 levels Dataset Description: <p>These data show cellular characterizations of two strains of <em>Emiliania huxleyi</em> cultured semi-continuously over a period 13-14 days under three different pCO2 concentrations (400 ppmv, 750 ppmv, and 1000 ppmv).&nbsp; Cellular characterization measurements were taken throughout the course of the experiments, resulting in a time course data set.&nbsp; CO2 chemistry was also monitored over the course of the experiment.&nbsp; Cellular characterizations included: intrinsic growth rate, cell volume, cellular particulate organic carbon and nitrogen, cellular particulate inorganic carbon, cellular chlorophyll <em>a</em>, and cellular particulate dimethylsulfoniopropionate.</p>
<p><strong><em>Emiliania huxleyi</em> strains:</strong></p>
<p>Strain NCMA 2668, calcifying phenotype, isolated from Gulf of Maine 2002<br />
Strain NCMA 374, non-calcifying phenotype, isolated from Gulf of Maine 1990</p>
<p><strong>Related Datasets:</strong><br />
<a href="http://www.bco-dmo.org/dataset/521347">Emiliania huxleyi Chl-a, POC, cell volumes</a><br />
<a href="http://www.bco-dmo.org/dataset/521403">Emiliania huxleyi CN content</a><br />
<a href="http://www.bco-dmo.org/dataset/521279">Emiliania huxleyi dilution calculations</a><br />
<a href="http://www.bco-dmo.org/dataset/520724">Emiliania huxleyi growth rates</a></p> Methods and Sampling: <p><strong>Culturing conditions:</strong></p>
<p>Cultures of <em>E. huxleyi </em>StrainNCMA 2668 and 374 were innoculated at low cell density into media prepared from autoclaved filtered seawater with f/50 nutrient ammendment.&nbsp; Cell populations were allowed to acclimate for approximately five generations, until cell density neared levels likely to significantly change the pH/pCO<sub>2</sub>.&nbsp; Daily dilutions of cultures with pre-equilibrated media kept cell density low (&lt;1x10<sup>5</sup> cells/ml), ensured cells remained in exponential growth phase and prevented excessive drawdown of nutrients and CO<sub>2</sub>.&nbsp; Cell density was determined by flow cytometry (model described below) and each flask was diluted with media that was continuously sparged with air containing 400, 750 or 1000 ppm CO<sub>2</sub>.&nbsp; Air mixtures were created using CO<sub>2</sub> free air (Powerex air compressor, and Twin Towers CO2 scrubber) and pure CO<sub>2</sub> (Airgas) combined using a system of mass flow controllers (Sierra Instruments) and verified using a non-dispersive infrared CO<sub>2</sub> sensor (Licor 820).&nbsp; Cultures were maintained in 1 liter polycarbonate flasks at 15<sup>°</sup>C under a 12/12 light dark cycle.&nbsp; Replicates (n=5) were placed in Plexiglas chambers which were supplied with a flow of the appropriate air mixture for each treatment.&nbsp; Preliminary experiments showed that gas exchange across the air/water surface significantly helped to maintain the target pCO2 in cultures without the mechanical disturbance of bubbling.&nbsp; Sedimentation was minimized by gentle mixing of the cultures by rotation of the bottles twice a day, during sampling and dilution.&nbsp; Cell densities ranged between about 30,000 cells/ml after dilutions to 80,000 cells/ml on the following day.&nbsp; The culture volume that was removed was used for analyses, and replaced with pre-equilibrated media.&nbsp; Cultures were maintianed in this fashion for 14 days.&nbsp; This experiment was carried out twice, in 2011 and 2012.&nbsp;</p>
<p><strong>CO<sub>2</sub> chemistry: </strong></p>
<p>pCO<sub>2 </sub>throughout the course of the experiment was calculated using CO2sys program, with pH and total alkalinity as variables and using Millero constants.&nbsp; pH was measured using a Metrohm 888 Titrando with a Metrohm Ecotrode combined electrode calibrated with TRIS and AMP buffers on the total H<sup>+</sup> ion pH scale.</p>
<p>Total alkalinity was measured with a Metrohm 888 Titrando with seawater buffers prepared by combining prepared sea salts and HCl with 2-amino-2-hydroxymethyl-1,3-propanediol and 2-aminopyridine.</p>
<p><strong>Intrinsic growth rate:</strong></p>
<p>Daily cell counts were made using a BD FACSCalibur flow cytometer.&nbsp; Manual counts were done on select samples using a hemocytometer.&nbsp; Manual counts were consistently within 5% of flow cytometry counts.&nbsp; Intrinsic growth rate was calculated using exponential growth equation.</p>
<p class="rteindent1"><strong>Additional results</strong>:<br />
<a href="http://dmoserv3.whoi.edu/data_docs/Ehux_pCO2/Ehux growth rates ANOVA_ver3July2014.pdf" target="_blank">Stats testing differences in growth between strains: ANOVA</a></p>
<p><strong>Cell size:</strong></p>
<p>Live cells were imaged using an Olympus CH30 compound microscope networked to a Photometric CoolSNAP camera.&nbsp; Cell diameter was measured using ImageJ software, and cell volume was calculated using standard geometric equations.</p>
<p><strong>Cellular chlorophyll <em>a</em>:</strong></p>
<p>Chlorophyll <em>a</em> samples were extracted for 24 h in acetone under -20 °C.&nbsp; Chlorophyll <em>a</em> was measured using a Turner Designs 10-AU fluorometer.&nbsp; The acidifying equations of Parsons were used to convert raw fluorescence into chlorophyll a concentration.</p>
<p><strong>Cellular carbon and nitrogen:</strong></p>
<p>Samples for cellular particulate carbon and nitrogen were analyzed using a CE Elantech Flash EA 1112 elemental analyzer.&nbsp; In all analysis blanks were run, and internal standards were inserted between samples, and remained within 1% of standard curve.&nbsp; For the calcifying strain (2668), samples were acid fumed for 24 h to drive off PIC.&nbsp; Values of organic carbon were subtracted from total carbon to yield cellular particulate inorganic carbon.</p>
<p><strong>Cellular particulate DMSP:</strong></p>
<p>A Shimadzu GC-14A gas chromatograph was used to measure cellular particulate DMSP. Standards were prepared using DMSP Cl.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0961229 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0961229
completed
M. Brady Olson
Western Washington University - Shannon Point Marine Center
(360) 650-7400
1900 Shannon Point Rd.
Anacortes
WA
98221
United States
Brady.Olson@wwu.edu
pointOfContact
Brooke Love
Western Washington University
(360) 650-2894
516 High St. Mail Stop 9181
Bellingham
WA
98225
United States
brooke.love@wwu.edu
pointOfContact
Suzanne Strom
Western Washington University - Shannon Point Marine Center
360-293-2188
Shannon Point Marine Center 1900 Shannon Point Rd
Anacortes
WA
98221
USA
suzanne.strom@wwu.edu
pointOfContact
asNeeded
Dataset Version: 2014-07-03
Unknown
strain
date
sample
peak_area
peak_area_sqrt
DMSP_per_ml_filt
cells_per_ml
fmol_DMSP_per_cell
pg_DMSP_per_cell
mean_DMSP_per_cell
days
camera
CHN_EA
CO2 Analyzer
Flow Cytometer
Fluorometer
Gas Chromatograph
MFC
compound microscope
spectrophotometer
Automatic titrator
theme
None, User defined
No BCO-DMO term
date
sample identification
days
featureType
BCO-DMO Standard Parameters
Camera
CHN Elemental Analyzer
CO2 Analyzer
Flow Cytometer
Fluorometer
Gas Chromatograph
Mass Flow Controller
Microscope - Optical
Spectrophotometer
Titrator
instrument
BCO-DMO Standard Instruments
Lab_Olson_B
service
Deployment Activity
Bellingham, WA
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Planktonic interactions in a changing ocean: Biological responses of Emiliania huxleyi to elevated pCO2 and their effects on microzooplankton
https://osprey.bco-dmo.org/project/517694
Planktonic interactions in a changing ocean: Biological responses of Emiliania huxleyi to elevated pCO2 and their effects on microzooplankton
<p><em><strong>Description from NSF award abstract:</strong></em><br />
The calcifying Haptophyte <em>Emiliania huxleyi </em>appears to be acutely sensitive to the rising concentration of ocean pCO2. Documented responses by <em>E. huxleyi </em>to elevated pCO2 include modifications to their calcification rate and cell size, malformation of coccoliths, elevated growth rates, increased organic carbon production, lowering of PIC:POC ratios, and elevated production of the active climate gas DMS. Changes in these parameters are mechanisms known to elicit alterations in grazing behavior by microzooplankton, the oceans dominant grazer functional group. The investigators hypothesize that modifications to the physiology and biochemistry of calcifying and non-calcifying Haptophyte <em>Emiliania huxleyi </em>in response to elevated pCO2 will precipitate alterations in microzooplankton grazing dynamics. To test this hypothesis, they will conduct controlled laboratory experiments where several strains of <em>E. huxleyi</em> are grown at several CO2 concentrations. After careful characterization of the biochemical and physiological responses of the <em>E. huxleyi</em> strains to elevated pCO2, they will provide these strains as food to several ecologically-important microzooplankton and document grazing dynamics.<em> E. huxleyi</em> is an ideal organism for the study of phytoplankton and microzooplankton responses to rising anthropogenic CO2, the effects of which in the marine environment are called ocean acidification; <em>E. huxleyi </em>is biogeochemically important, is well studied, numerous strains are in culture that exhibit variation in the parameters described above, and they are readily fed upon by ecologically important microzooplankton.</p>
<p>The implications of changes in microzooplankton grazing for carbon cycling, specifically CaCO3 export, DMS production, nutrient regeneration in surface waters, and carbon transfer between trophic levels are profound, as this grazing, to a large degree, regulates all these processes. <em>E. huxleyi </em>is a model prey organism because it is one of the most biogeochemically influential global phytoplankton. It forms massive seasonal blooms, contributes significantly to marine inorganic and organic carbon cycles, is a large producer of the climatically active gas DMS, and is a source of organic matter for trophic levels both above and below itself. The planned controlled study will increase our knowledge of the mechanisms that drive patterns of change between trophic levels, thus providing a wider array of tools necessary to understand the complex nature of ocean acidification field studies, where competing variables can confound precise interpretation.</p>
E Hux Response to pCO2
largerWorkCitation
project
eng; USA
biota
oceans
Bellingham, WA
2014-07-03
0
BCO-DMO catalogue of parameters from Experimental results: cellular particulate DMSP from E. huxleyi at 3 pCO2 levels, 2011-2012 (E Hux Response to pCO2 project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/521331.rdf
Name: strain
Units: unitless
Description: <p>Emiliania huxleyi stain id</p>
http://lod.bco-dmo.org/id/dataset-parameter/521332.rdf
Name: date
Units: yyyy-mm-dd
Description: <p>date of analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/521333.rdf
Name: sample
Units: unitless
Description: <p>sample id</p>
http://lod.bco-dmo.org/id/dataset-parameter/521334.rdf
Name: peak_area
Units: unitless
Description: <p>area under the chromatogram peak for DMSP</p>
http://lod.bco-dmo.org/id/dataset-parameter/521335.rdf
Name: peak_area_sqrt
Units: unitless
Description: <p>square root of peak area</p>
http://lod.bco-dmo.org/id/dataset-parameter/521336.rdf
Name: DMSP_per_ml_filt
Units: micromoles/milliliter
Description: <p>concentration of DMSP</p>
http://lod.bco-dmo.org/id/dataset-parameter/521337.rdf
Name: cells_per_ml
Units: cells/milliliter
Description: <p>concentration of cells</p>
http://lod.bco-dmo.org/id/dataset-parameter/521338.rdf
Name: fmol_DMSP_per_cell
Units: femtomoles/cell
Description: <p>concentration of DMSP per cell</p>
http://lod.bco-dmo.org/id/dataset-parameter/521339.rdf
Name: pg_DMSP_per_cell
Units: picograms/cell
Description: <p>concentration of DMSP per cell</p>
http://lod.bco-dmo.org/id/dataset-parameter/521340.rdf
Name: mean_DMSP_per_cell
Units: picograms/cell
Description: <p>average concentration of DMSP per cell</p>
http://lod.bco-dmo.org/id/dataset-parameter/521341.rdf
Name: days
Units: days
Description: <p>days since initial dilution</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
12445
https://datadocs.bco-dmo.org/file/OJJGvNOTwwq2qJ/DMSP.csv
DMSP.csv
Primary data file for dataset ID 521312
download
https://osprey.bco-dmo.org/dataset/521312/data/download
download
onLine
dataset
<p><strong>Culturing conditions:</strong></p>
<p>Cultures of <em>E. huxleyi </em>StrainNCMA 2668 and 374 were innoculated at low cell density into media prepared from autoclaved filtered seawater with f/50 nutrient ammendment.&nbsp; Cell populations were allowed to acclimate for approximately five generations, until cell density neared levels likely to significantly change the pH/pCO<sub>2</sub>.&nbsp; Daily dilutions of cultures with pre-equilibrated media kept cell density low (&lt;1x10<sup>5</sup> cells/ml), ensured cells remained in exponential growth phase and prevented excessive drawdown of nutrients and CO<sub>2</sub>.&nbsp; Cell density was determined by flow cytometry (model described below) and each flask was diluted with media that was continuously sparged with air containing 400, 750 or 1000 ppm CO<sub>2</sub>.&nbsp; Air mixtures were created using CO<sub>2</sub> free air (Powerex air compressor, and Twin Towers CO2 scrubber) and pure CO<sub>2</sub> (Airgas) combined using a system of mass flow controllers (Sierra Instruments) and verified using a non-dispersive infrared CO<sub>2</sub> sensor (Licor 820).&nbsp; Cultures were maintained in 1 liter polycarbonate flasks at 15<sup>°</sup>C under a 12/12 light dark cycle.&nbsp; Replicates (n=5) were placed in Plexiglas chambers which were supplied with a flow of the appropriate air mixture for each treatment.&nbsp; Preliminary experiments showed that gas exchange across the air/water surface significantly helped to maintain the target pCO2 in cultures without the mechanical disturbance of bubbling.&nbsp; Sedimentation was minimized by gentle mixing of the cultures by rotation of the bottles twice a day, during sampling and dilution.&nbsp; Cell densities ranged between about 30,000 cells/ml after dilutions to 80,000 cells/ml on the following day.&nbsp; The culture volume that was removed was used for analyses, and replaced with pre-equilibrated media.&nbsp; Cultures were maintianed in this fashion for 14 days.&nbsp; This experiment was carried out twice, in 2011 and 2012.&nbsp;</p>
<p><strong>CO<sub>2</sub> chemistry: </strong></p>
<p>pCO<sub>2 </sub>throughout the course of the experiment was calculated using CO2sys program, with pH and total alkalinity as variables and using Millero constants.&nbsp; pH was measured using a Metrohm 888 Titrando with a Metrohm Ecotrode combined electrode calibrated with TRIS and AMP buffers on the total H<sup>+</sup> ion pH scale.</p>
<p>Total alkalinity was measured with a Metrohm 888 Titrando with seawater buffers prepared by combining prepared sea salts and HCl with 2-amino-2-hydroxymethyl-1,3-propanediol and 2-aminopyridine.</p>
<p><strong>Intrinsic growth rate:</strong></p>
<p>Daily cell counts were made using a BD FACSCalibur flow cytometer.&nbsp; Manual counts were done on select samples using a hemocytometer.&nbsp; Manual counts were consistently within 5% of flow cytometry counts.&nbsp; Intrinsic growth rate was calculated using exponential growth equation.</p>
<p class="rteindent1"><strong>Additional results</strong>:<br />
<a href="http://dmoserv3.whoi.edu/data_docs/Ehux_pCO2/Ehux growth rates ANOVA_ver3July2014.pdf" target="_blank">Stats testing differences in growth between strains: ANOVA</a></p>
<p><strong>Cell size:</strong></p>
<p>Live cells were imaged using an Olympus CH30 compound microscope networked to a Photometric CoolSNAP camera.&nbsp; Cell diameter was measured using ImageJ software, and cell volume was calculated using standard geometric equations.</p>
<p><strong>Cellular chlorophyll <em>a</em>:</strong></p>
<p>Chlorophyll <em>a</em> samples were extracted for 24 h in acetone under -20 °C.&nbsp; Chlorophyll <em>a</em> was measured using a Turner Designs 10-AU fluorometer.&nbsp; The acidifying equations of Parsons were used to convert raw fluorescence into chlorophyll a concentration.</p>
<p><strong>Cellular carbon and nitrogen:</strong></p>
<p>Samples for cellular particulate carbon and nitrogen were analyzed using a CE Elantech Flash EA 1112 elemental analyzer.&nbsp; In all analysis blanks were run, and internal standards were inserted between samples, and remained within 1% of standard curve.&nbsp; For the calcifying strain (2668), samples were acid fumed for 24 h to drive off PIC.&nbsp; Values of organic carbon were subtracted from total carbon to yield cellular particulate inorganic carbon.</p>
<p><strong>Cellular particulate DMSP:</strong></p>
<p>A Shimadzu GC-14A gas chromatograph was used to measure cellular particulate DMSP. Standards were prepared using DMSP Cl.</p>
Specified by the Principal Investigator(s)
<p>Data are compiled to show averages and standard deviations by day and treatment.&nbsp;</p>
<p><strong>Relevant References:</strong></p>
<p>Wuori, Tristen, "The effects of elevated PCO2 on the physiology of Emiliania huxleyi" (2012). Western Washington University Masters Thesis Collection. Paper 235. <a href="http://cedar.wwu.edu/wwuet/235/" target="_blank">http://cedar.wwu.edu/wwuet/235/</a></p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
camera
camera
PI Supplied Instrument Name: camera PI Supplied Instrument Description:Photometrics CoolSNAP camera, networked to microscope Instrument Name: Camera Instrument Short Name:camera Instrument Description: All types of photographic equipment including stills, video, film and digital systems. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/311/
CHN_EA
CHN_EA
PI Supplied Instrument Name: CHN_EA PI Supplied Instrument Description:CE Elantech Flash EA 1112 elemental analyzer Instrument Name: CHN Elemental Analyzer Instrument Short Name:CHN_EA Instrument Description: A CHN Elemental Analyzer is used for the determination of carbon, hydrogen, and nitrogen content in organic and other types of materials, including solids, liquids, volatile, and viscous samples.
CO2 Analyzer
CO2 Analyzer
PI Supplied Instrument Name: CO2 Analyzer PI Supplied Instrument Description:Licor 820: a non-dispersive infrared CO2 sensor Instrument Name: CO2 Analyzer Instrument Short Name:CO2 Analyzer Instrument Description: Measures atmospheric carbon dioxide (CO2) concentration. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/382/
Flow Cytometer
Flow Cytometer
PI Supplied Instrument Name: Flow Cytometer PI Supplied Instrument Description:BD FACSCalibur flow cytometer Instrument Name: Flow Cytometer Instrument Short Name:Flow Cytometer Instrument Description: Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells.
(from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm) Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB37/
Fluorometer
Fluorometer
PI Supplied Instrument Name: Fluorometer PI Supplied Instrument Description:Turner Designs 10-AU fluorometer Instrument Name: Fluorometer Instrument Short Name:Fluorometer Instrument Description: A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/113/
Gas Chromatograph
Gas Chromatograph
PI Supplied Instrument Name: Gas Chromatograph PI Supplied Instrument Description:Shimadzu GC-14A gas chromatograph Instrument Name: Gas Chromatograph Instrument Short Name:Gas Chromatograph Instrument Description: Instrument separating gases, volatile substances, or substances dissolved in a volatile solvent by transporting an inert gas through a column packed with a sorbent to a detector for assay. (from SeaDataNet, BODC) Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB02/
MFC
MFC
PI Supplied Instrument Name: MFC PI Supplied Instrument Description:Sierra Instruments Instrument Name: Mass Flow Controller Instrument Short Name:MFC Instrument Description: Mass Flow Controller (MFC) - A device used to measure and control the flow of fluids and gases
compound microscope
compound microscope
PI Supplied Instrument Name: compound microscope PI Supplied Instrument Description:Olympus CH30 compound microscope networked to a Photometric CoolSNAP camera Instrument Name: Microscope - Optical Instrument Short Name: Instrument Description: Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope". Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/
spectrophotometer
spectrophotometer
PI Supplied Instrument Name: spectrophotometer PI Supplied Instrument Description:Agilent 5480 UV-VIS spectrophotometer (+/- 0.02) Instrument Name: Spectrophotometer Instrument Short Name:Spectrophotometer Instrument Description: An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/
Automatic titrator
Automatic titrator
PI Supplied Instrument Name: Automatic titrator PI Supplied Instrument Description:Metrohm 888 Titrando with a Metrohm Ecotrode combined electrode, calibrated with TRIS and AMP buffers on the total H+ ion pH scale. Instrument Name: Titrator Instrument Short Name:Titrator Instrument Description: Titrators are instruments that incrementally add quantified aliquots of a reagent to a sample until the end-point of a chemical reaction is reached. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB12/
Deployment: Lab_Olson_B
Lab_Olson_B
WWU
laboratory
WWU
laboratory