1b:I[42426,["/_next/static/immutable/chunks/1xctgzqre-wsy.js","/_next/static/immutable/chunks/06_-wz6pdtp2b.js","/_next/static/immutable/chunks/30e52g49hg40p.js","/_next/static/immutable/chunks/02yq6tul2z-oh.js","/_next/static/immutable/chunks/10bx_ec2vj7n_.js","/_next/static/immutable/chunks/33bfn03fo8xnt.js","/_next/static/immutable/chunks/0tfjf78scu61b.js","/_next/static/immutable/chunks/31xnvej4n1bi9.js"],"default"] 1c:T52d,

These data show cellular characterizations of two strains of Emiliania huxleyi cultured semi-continuously over a period 13-14 days under three different pCO2 concentrations (400 ppmv, 750 ppmv, and 1000 ppmv). Cellular characterization measurements were taken throughout the course of the experiments, resulting in a time course data set. CO2 chemistry was also monitored over the course of the experiment. Cellular characterizations included: intrinsic growth rate, cell volume, cellular particulate organic carbon and nitrogen, cellular particulate inorganic carbon, cellular chlorophyll a, and cellular particulate dimethylsulfoniopropionate.

Emiliania huxleyi strains:Strain NCMA 2668, calcifying phenotype, isolated from Gulf of Maine 2002

Strain NCMA 374, non-calcifying phenotype, isolated from Gulf of Maine 1990

1d:T1185,

Culturing conditions:

Cultures of E. huxleyi StrainNCMA 2668 and 374 were innoculated at low cell density into media prepared from autoclaved filtered seawater with f/50 nutrient ammendment. Cell populations were allowed to acclimate for approximately five generations, until cell density neared levels likely to significantly change the pH/pCO₂. Daily dilutions of cultures with pre-equilibrated media kept cell density low (<1x10⁵ cells/ml), ensured cells remained in exponential growth phase and prevented excessive drawdown of nutrients and CO₂. Cell density was determined by flow cytometry (model described below) and each flask was diluted with media that was continuously sparged with air containing 400, 750 or 1000 ppm CO₂. Air mixtures were created using CO₂ free air (Powerex air compressor, and Twin Towers CO2 scrubber) and pure CO₂ (Airgas) combined using a system of mass flow controllers (Sierra Instruments) and verified using a non-dispersive infrared CO₂ sensor (Licor 820). Cultures were maintained in 1 liter polycarbonate flasks at 15^°C under a 12/12 light dark cycle. Replicates (n=5) were placed in Plexiglas chambers which were supplied with a flow of the appropriate air mixture for each treatment. Preliminary experiments showed that gas exchange across the air/water surface significantly helped to maintain the target pCO2 in cultures without the mechanical disturbance of bubbling. Sedimentation was minimized by gentle mixing of the cultures by rotation of the bottles twice a day, during sampling and dilution. Cell densities ranged between about 30,000 cells/ml after dilutions to 80,000 cells/ml on the following day. The culture volume that was removed was used for analyses, and replaced with pre-equilibrated media. Cultures were maintianed in this fashion for 14 days. This experiment was carried out twice, in 2011 and 2012.

CO₂ chemistry:

pCO₂throughout the course of the experiment was calculated using CO2sys program, with pH and total alkalinity as variables and using Millero constants. pH was measured using a Metrohm 888 Titrando with a Metrohm Ecotrode combined electrode calibrated with TRIS and AMP buffers on the total H⁺ ion pH scale.

Total alkalinity was measured with a Metrohm 888 Titrando with seawater buffers prepared by combining prepared sea salts and HCl with 2-amino-2-hydroxymethyl-1,3-propanediol and 2-aminopyridine.

Intrinsic growth rate:

Daily cell counts were made using a BD FACSCalibur flow cytometer. Manual counts were done on select samples using a hemocytometer. Manual counts were consistently within 5% of flow cytometry counts. Intrinsic growth rate was calculated using exponential growth equation.

Additional results:
Stats testing differences in growth between strains: ANOVA

Cell size:

Live cells were imaged using an Olympus CH30 compound microscope networked to a Photometric CoolSNAP camera. Cell diameter was measured using ImageJ software, and cell volume was calculated using standard geometric equations.

Cellular chlorophyll a:

Chlorophyll a samples were extracted for 24 h in acetone under -20 °C. Chlorophyll a was measured using a Turner Designs 10-AU fluorometer. The acidifying equations of Parsons were used to convert raw fluorescence into chlorophyll a concentration.

Cellular carbon and nitrogen:

Samples for cellular particulate carbon and nitrogen were analyzed using a CE Elantech Flash EA 1112 elemental analyzer. In all analysis blanks were run, and internal standards were inserted between samples, and remained within 1% of standard curve. For the calcifying strain (2668), samples were acid fumed for 24 h to drive off PIC. Values of organic carbon were subtracted from total carbon to yield cellular particulate inorganic carbon.

Cellular particulate DMSP:

A Shimadzu GC-14A gas chromatograph was used to measure cellular particulate DMSP. Standards were prepared using DMSP Cl.

1e:Ta3e,Description from NSF award abstract: The calcifying Haptophyte Emiliania huxleyi appears to be acutely sensitive to the rising concentration of ocean pCO2. Documented responses by E. huxleyi to elevated pCO2 include modifications to their calcification rate and cell size, malformation of coccoliths, elevated growth rates, increased organic carbon production, lowering of PIC:POC ratios, and elevated production of the active climate gas DMS. Changes in these parameters are mechanisms known to elicit alterations in grazing behavior by microzooplankton, the oceans dominant grazer functional group. The investigators hypothesize that modifications to the physiology and biochemistry of calcifying and non-calcifying Haptophyte Emiliania huxleyi in response to elevated pCO2 will precipitate alterations in microzooplankton grazing dynamics. To test this hypothesis, they will conduct controlled laboratory experiments where several strains of E. huxleyi are grown at several CO2 concentrations. After careful characterization of the biochemical and physiological responses of the E. huxleyi strains to elevated pCO2, they will provide these strains as food to several ecologically-important microzooplankton and document grazing dynamics. E. huxleyi is an ideal organism for the study of phytoplankton and microzooplankton responses to rising anthropogenic CO2, the effects of which in the marine environment are called ocean acidification; E. huxleyi is biogeochemically important, is well studied, numerous strains are in culture that exhibit variation in the parameters described above, and they are readily fed upon by ecologically important microzooplankton. The implications of changes in microzooplankton grazing for carbon cycling, specifically CaCO3 export, DMS production, nutrient regeneration in surface waters, and carbon transfer between trophic levels are profound, as this grazing, to a large degree, regulates all these processes. E. huxleyi is a model prey organism because it is one of the most biogeochemically influential global phytoplankton. It forms massive seasonal blooms, contributes significantly to marine inorganic and organic carbon cycles, is a large producer of the climatically active gas DMS, and is a source of organic matter for trophic levels both above and below itself. The planned controlled study will increase our knowledge of the mechanisms that drive patterns of change between trophic levels, thus providing a wider array of tools necessary to understand the complex nature of ocean acidification field studies, where competing variables can confound precise interpretation.6:["$","$L1b",null,{"dataset":{"_id":"521347","description":"$1c","problem":"","acquisition":"$1d","processing":"

Data are compiled to show averages and standard deviations by day and treatment.

Relevant References:

Wuori, T. 2012. The effects of elevated pCO₂on the physiology of Emiliania huxleyi. M.S. Thesis, Western Washington University. http://cedar.wwu.edu/wwuet/235/

\n","bcodmoProcessing":"","title":"Experimental results: Chlorophyll-a, POC, and cell volume of E. huxleyi at 3 pCO2 levels, 2011-2012 (E Hux Response to pCO2 project)","nickname":"Emiliania huxleyi Chl-a, POC, cell volumes","dataUrl":"","currentState":"Final no updates expected","currentStateId":"7","validated":true,"restricted":false,"version":"2014-07-03","version_date":"2014-07-03T04:00:00.000Z","abstract":"","citation":"Olson, M. Brady, Strom, S., Love, B. (2014) Experimental results: Chlorophyll-a, POC, and cell volume of E. huxleyi at 3 pCO2 levels, 2011-2012 (E Hux Response to pCO2 project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2014-07-03) Version Date 2014-07-03 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/521347 [access date]","citationParts":{"identifier":"http://lod.bco-dmo.org/id/dataset/521347","versionDate":"2014-07-03","versionId":"2014-07-03","year":"2014","title":"Experimental results: Chlorophyll-a, POC, and cell volume of E. huxleyi at 3 pCO2 levels, 2011-2012 (E Hux Response to pCO2 project)","authors":"Olson, M. Brady, Strom, S., Love, B.","publisher":"Biological and Chemical Oceanography Data Management Office (BCO-DMO)."},"doi":"","release_date":null,"field_dataset_location":"","temporal_extent":{"start":null,"end":null},"temporal":{"resolution":"","type":"","start":{"value":"","label":""},"end":{"value":"","label":""}},"spatial_extent":{"shape":"","center":{"latitude":0,"longitude":0},"wkt":"","north":0,"east":0,"south":0,"west":0},"dataset_type":["experimental"],"keywords":["biota","oceans"],"data_files":[{"id":"900189","title":"chl_poc.csv","url":"https://datadocs.bco-dmo.org/dataset/521347/file/k66gxAyhYyP3l1/chl_poc.csv","showTableUrl":"https://www.bco-dmo.org/dataset/521347/data/view/900189","description":"Primary data file for dataset ID 521347","filename":"chl_poc.csv","bytesize":26197,"checksum":{"value":"425a6cc8e48d42580f6753c4b7d72293","Algorithm":{"_id":"190","name":"MD5"}},"mimetype":"text/csv","fileType":{"_id":"192","name":"Comma Separated Values (.csv)"},"isErddap":"","date":"","isPublicDuringEmbargo":false,"primaryDataFile":true,"showTable":true,"restricted":false}],"supplemental_files":[],"roles":[{"role":{"_id":"138","name":"Principal Investigator"},"performedBy":{"_id":"51528","name":"M. Brady Olson"},"performedAtOrganization":{"_id":"291","name":"Western Washington University - Shannon Point Marine Center"}},{"role":{"_id":"140","name":"Co-Principal Investigator"},"performedBy":{"_id":"51527","name":"Brooke Love"},"performedAtOrganization":{"_id":"50","name":"Western Washington University"}},{"role":{"_id":"140","name":"Co-Principal Investigator"},"performedBy":{"_id":"50471","name":"Suzanne Strom"},"performedAtOrganization":{"_id":"291","name":"Western Washington University - Shannon Point Marine Center"}},{"role":{"_id":"128","name":"Student"},"performedBy":{"_id":"528910","name":"Tristen Wuori"},"performedAtOrganization":{"_id":"50","name":"Western Washington University"}},{"role":{"_id":"133","name":"Contact"},"performedBy":{"_id":"51528","name":"M. Brady Olson"},"performedAtOrganization":{"_id":"291","name":"Western Washington University - Shannon Point Marine Center"}},{"role":{"_id":"145","name":"BCO-DMO Data Manager"},"performedBy":{"_id":"50396","name":"Nancy Copley"},"performedAtOrganization":{"_id":"191","name":"Woods Hole Oceanographic Institution"}}],"deployments":[{"_id":"521277","name":"Lab_Olson_B","synonyms":[],"dates":{"start":"2011-03-31T00:00:00.000Z","end":"2016-09-15T00:00:00.000Z"},"platform":{"_id":"54249","name":"WWU"},"chiefScientists":[]}],"funding":[{"_id":"517693","name":"OCE-0961229","fundingSource":{"_id":"355","name":"NSF Division of Ocean Sciences"},"url":"https://www.nsf.gov/awardsearch/show-award?AWD_ID=0961229"}],"instruments":[{"_id":"521363","name":"camera","description":"Photometrics CoolSNAP camera, networked to microscope","type_id":"520","type_name":"Camera","type_desc":"All types of photographic equipment including stills, video, film and digital systems.","type_acronym":"camera"},{"_id":"521364","name":"CHN_EA","description":"CE Elantech Flash EA 1112 elemental analyzer","type_id":"625","type_name":"CHN Elemental Analyzer","type_desc":"A CHN Elemental Analyzer is used for the determination of carbon, hydrogen, and nitrogen content in organic and other types of materials, including solids, liquids, volatile, and viscous samples.","type_acronym":"CHN_EA"},{"_id":"521357","name":"CO2 Analyzer","description":"Licor 820: a non-dispersive infrared CO2 sensor","type_id":"491476","type_name":"CO2 Analyzer","type_desc":"Measures atmospheric carbon dioxide (CO2) concentration.","type_acronym":"CO2 Analyzer"},{"_id":"521358","name":"Flow Cytometer","description":"BD FACSCalibur flow cytometer","type_id":"660","type_name":"Flow Cytometer","type_desc":"Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells. Description from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm","type_acronym":"Flow Cytometer"},{"_id":"521361","name":"Fluorometer","description":"Turner Designs 10-AU fluorometer","type_id":"484","type_name":"Fluorometer","type_desc":"A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ.","type_acronym":"Fluorometer"},{"_id":"521365","name":"Gas Chromatograph","description":"Shimadzu GC-14A gas chromatograph","type_id":"661","type_name":"Gas Chromatograph","type_desc":"Instrument separating gases, volatile substances, or substances dissolved in a volatile solvent by transporting an inert gas through a column packed with a sorbent to a detector for assay. (from SeaDataNet, BODC)","type_acronym":"Gas Chromatograph"},{"_id":"521359","name":"MFC","description":"Sierra Instruments","type_id":"712","type_name":"Mass Flow Controller","type_desc":"Mass Flow Controller (MFC) - A device used to measure and control the flow of fluids and gases","type_acronym":"MFC"},{"_id":"521362","name":"compound microscope","description":"Olympus CH30 compound microscope networked to a Photometric CoolSNAP camera","type_id":"708","type_name":"Microscope - Optical","type_desc":"Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a \"light microscope\".","type_acronym":""},{"_id":"521360","name":"spectrophotometer","description":"Agilent 5480 UV-VIS spectrophotometer (+/- 0.02)","type_id":"707","type_name":"Spectrophotometer","type_desc":"An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples.","type_acronym":"Spectrophotometer"},{"_id":"521356","name":"Automatic titrator","description":"Metrohm 888 Titrando with a Metrohm Ecotrode combined electrode, calibrated with TRIS and AMP buffers on the total H+ ion pH scale.","type_id":"682","type_name":"Titrator","type_desc":"Titrators are instruments that incrementally add quantified aliquots of a reagent to a sample until the end-point of a chemical reaction is reached.","type_acronym":"Titrator"}],"parameters":[{"_id":"521367","name":"strain","format":"","frictionlessType":"","description":"

Emiliania huxleyi stain id

","units":{"_id":"","name":"unitless"},"type":{"_id":"1073","name":"no_bcodmo_term","description":"

An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"521368","name":"date","format":"","frictionlessType":"","description":"

date of analysis

","units":{"_id":"","name":"yyyy-mm-dd"},"type":{"_id":"1070","name":"date","description":"

date; generally reported in GMT as YYYYMMDD (year; month; day); also as MMDD (month; day); EqPac dates are local Hawaii time. ISO_Date format is YYYY-MM-DD (http://www.iso.org/iso/home/standards/iso8601.htm)

"}},{"_id":"521369","name":"sample","format":"","frictionlessType":"","description":"

sample id

","units":{"_id":"","name":"unitless"},"type":{"_id":"960","name":"sample","description":"

unique sample identification or number; any combination of alpha numeric characters; precise definition is file dependent

"}},{"_id":"521370","name":"Fo","format":"","frictionlessType":"","description":"

fluorometric reading of the non acidified sample

","units":{"_id":"","name":"unitless"},"type":{"_id":"1073","name":"no_bcodmo_term","description":"

An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"521371","name":"Fa","format":"","frictionlessType":"","description":"

fluorometric reading of the acidified sample

","units":{"_id":"","name":"unitless"},"type":{"_id":"1073","name":"no_bcodmo_term","description":"

An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"521372","name":"vol_filt","format":"","frictionlessType":"","description":"

volume filtered

","units":{"_id":"","name":"ml"},"type":{"_id":"1040","name":"vol_filt","description":"

volume of water filtered; in this form generally applies to the volume of water filtered during plankton tows; however; this parameter name is also used for a variety of non plankton sampling and the units can vary, e.g. US JGOFS EqPac Bishop MULVFS sampler vol_filt is reported in liters.

"}},{"_id":"521373","name":"chl_a","format":"","frictionlessType":"","description":"

chorophyll-a concentration

","units":{"_id":"","name":"ug/ml"},"type":{"_id":"766","name":"chlorophyll a","description":"

Total chlorophyll a pigment. See dataset for units of measurement; often reported in: milligrams per cubic meter (mg/m3); micrograms/liter (ug/L); nanograms/liter (ng/L).

CAUTION Within JGOFS MERGED water column products: this parameter is derived from HPLC methodology and the units are nanograms/liter; however; this parameter name is also used for a variety of water and nonwater column sampling; derived via the fluorometric method; units will also vary.

"}},{"_id":"521374","name":"Fo_to_Fa","format":"","frictionlessType":"","description":"

ratio of chlorophyll-a to phaeopigment based on fluorometric readings of a non acidified (Fo) and acidified (Fa) samples

","units":{"_id":"","name":"unitless"},"type":{"_id":"1413","name":"Fo_to_Fa","description":"

ratio of chlorophyll-a to phaeopigment based on fluorometric readings of a non acidified (Fo) and acidified (Fa) samples

"}},{"_id":"521375","name":"days","format":"","frictionlessType":"","description":"

days since start of experiment

","units":{"_id":"","name":"days"},"type":{"_id":"473067","name":"days","description":"

Days as in days spent sampling or days post treatment for sampling

"}},{"_id":"521376","name":"cell_concentration","format":"","frictionlessType":"","description":"

Emiliania huxleyi cell concentration

","units":{"_id":"","name":"cells/ml"},"type":{"_id":"514901","name":"cell_concentration","description":"

Concentration of cells; often determined by spectrophotometry, flow cytometry, or using a microscope. May be referred to as \"cell count\" (cells per volume unit).

"}},{"_id":"521377","name":"chl_a_cell","format":"","frictionlessType":"","description":"

chorophyll-a concentration per E.hux cell

","units":{"_id":"","name":"picograms chla/cell"},"type":{"_id":"766","name":"chlorophyll a","description":"

Total chlorophyll a pigment. See dataset for units of measurement; often reported in: milligrams per cubic meter (mg/m3); micrograms/liter (ug/L); nanograms/liter (ng/L).

"}},{"_id":"521378","name":"chla_mean","format":"","frictionlessType":"","description":"

mean chorophyll-a concentration per E.hux cell for the pCO2 level

","units":{"_id":"","name":"picograms chla/cell"},"type":{"_id":"766","name":"chlorophyll a","description":"

Total chlorophyll a pigment. See dataset for units of measurement; often reported in: milligrams per cubic meter (mg/m3); micrograms/liter (ug/L); nanograms/liter (ng/L).

"}},{"_id":"521379","name":"chla_stdev","format":"","frictionlessType":"","description":"

standard deviation of chorophyll-a concentration per E.hux cell for the pCO2 level

","units":{"_id":"","name":"picograms chla/cell"},"type":{"_id":"1974","name":"standard deviation","description":"

Generic standard deviation value, taking on the units of the parameter that it is tied to.

"}},{"_id":"521380","name":"pcent_change","format":"","frictionlessType":"","description":"

percent change of chl-a from the control pCO2 level

","units":{"_id":"","name":"percent"},"type":{"_id":"1073","name":"no_bcodmo_term","description":"

An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"521381","name":"change_avg","format":"","frictionlessType":"","description":"

mean percent change of chl-a from the control to treatment pCO2 level

","units":{"_id":"","name":"percent"},"type":{"_id":"1073","name":"no_bcodmo_term","description":"

An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"521382","name":"change_stdev","format":"","frictionlessType":"","description":"

standard deviation of the percent change of chl-a from the control to treatment pCO2 level

","units":{"_id":"","name":"percent"},"type":{"_id":"1974","name":"standard deviation","description":"

Generic standard deviation value, taking on the units of the parameter that it is tied to.

"}},{"_id":"521383","name":"vol_cell","format":"","frictionlessType":"","description":"

average cell volume of replicates

","units":{"_id":"","name":"um3"},"type":{"_id":"1073","name":"no_bcodmo_term","description":"

An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"521384","name":"fg_chla_um3","format":"","frictionlessType":"","description":"

chlorophyll-a per unit volume

","units":{"_id":"","name":"fg chla/um3"},"type":{"_id":"766","name":"chlorophyll a","description":"

Total chlorophyll a pigment. See dataset for units of measurement; often reported in: milligrams per cubic meter (mg/m3); micrograms/liter (ug/L); nanograms/liter (ng/L).

"}},{"_id":"521385","name":"mean_fg_chla_um3","format":"","frictionlessType":"","description":"

average chlorophyll per unit volume

","units":{"_id":"","name":"fg chla/um3"},"type":{"_id":"766","name":"chlorophyll a","description":"

Total chlorophyll a pigment. See dataset for units of measurement; often reported in: milligrams per cubic meter (mg/m3); micrograms/liter (ug/L); nanograms/liter (ng/L).

"}},{"_id":"521386","name":"stdev_fg_chla_um3","format":"","frictionlessType":"","description":"

standard deviation of the average chlorophyll per unit volume

","units":{"_id":"","name":"fg chla/um3"},"type":{"_id":"1974","name":"standard deviation","description":"

Generic standard deviation value, taking on the units of the parameter that it is tied to.

"}},{"_id":"521387","name":"pcent_change_fg_chla_um3","format":"","frictionlessType":"","description":"

percent change of the chlorophyll per unit volume between the control and treatment pCO2

","units":{"_id":"","name":"percent"},"type":{"_id":"1073","name":"no_bcodmo_term","description":"

An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"521388","name":"mean_pcent_change_fg_chla_um3","format":"","frictionlessType":"","description":"

average of the percent change of the chlorophyll per unit volume between the control and treatment pCO2

","units":{"_id":"","name":"percent"},"type":{"_id":"1073","name":"no_bcodmo_term","description":"

An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"521389","name":"stdev_pcent_change_fg_chla_um3","format":"","frictionlessType":"","description":"

standard deviation of the percent change of the chlorophyll per unit volume between the control and treatment pCO2

","units":{"_id":"","name":"percent"},"type":{"_id":"1974","name":"standard deviation","description":"

Generic standard deviation value, taking on the units of the parameter that it is tied to.

"}},{"_id":"521390","name":"POC_cell","format":"","frictionlessType":"","description":"

particulate organic carbon per cell

","units":{"_id":"","name":"pg C/cell"},"type":{"_id":"934","name":"POC","description":"

Particulate Organic Carbon. Units may be micrograms/Liter, milligrams/Liter or milligrams/meter^3

"}},{"_id":"521391","name":"pg_chla_pg_C","format":"","frictionlessType":"","description":"

ratio of chlorophyll-a to carbon

","units":{"_id":"","name":"unitless"},"type":{"_id":"1073","name":"no_bcodmo_term","description":"

An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"521392","name":"mean_pg_chla_pg_C","format":"","frictionlessType":"","description":"

average ratio of chlorophyll-a to carbon

","units":{"_id":"","name":"unitless"},"type":{"_id":"1073","name":"no_bcodmo_term","description":"

An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"521393","name":"stdev_pg_chla_pg_C","format":"","frictionlessType":"","description":"

standard deviation of ratio of chlorophyll-a to carbon

","units":{"_id":"","name":"unitless"},"type":{"_id":"1974","name":"standard deviation","description":"

Generic standard deviation value, taking on the units of the parameter that it is tied to.

"}},{"_id":"521394","name":"pcent_change_pg_chla_pg_C","format":"","frictionlessType":"","description":"

percent change of chlorophyll-a per carbon between control and treatment pCO2 levels

","units":{"_id":"","name":"percent"},"type":{"_id":"1073","name":"no_bcodmo_term","description":"

An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"521395","name":"mean_pcent_change_pg_chla_pg_C","format":"","frictionlessType":"","description":"

average percent change of chlorophyll-a per carbon between control and treatment pCO2 levels

","units":{"_id":"","name":"percent"},"type":{"_id":"1073","name":"no_bcodmo_term","description":"

An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"521396","name":"stdev_pcent_change_pg_chla_pg_C","format":"","frictionlessType":"","description":"

standard deviation of percent change of chlorophyll-a per carbon between control and treatment pCO2 levels

","units":{"_id":"","name":"percent"},"type":{"_id":"1974","name":"standard deviation","description":"

Generic standard deviation value, taking on the units of the parameter that it is tied to.

"}},{"_id":"521397","name":"fmol_chla_cell","format":"","frictionlessType":"","description":"

chlorophyll-a per cell

","units":{"_id":"","name":"fmol chl/cell"},"type":{"_id":"766","name":"chlorophyll a","description":"

Total chlorophyll a pigment. See dataset for units of measurement; often reported in: milligrams per cubic meter (mg/m3); micrograms/liter (ug/L); nanograms/liter (ng/L).

"}},{"_id":"521398","name":"fmol_POC_cell","format":"","frictionlessType":"","description":"

particulate organic carbon per cell

","units":{"_id":"","name":"fmol POC/cell"},"type":{"_id":"934","name":"POC","description":"

Particulate Organic Carbon. Units may be micrograms/Liter, milligrams/Liter or milligrams/meter^3

"}},{"_id":"521399","name":"fmol_chl_POC","format":"","frictionlessType":"","description":"

ratio of chlorophyll-a to particulate organic carbon

","units":{"_id":"","name":"unitless"},"type":{"_id":"1073","name":"no_bcodmo_term","description":"

An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"521400","name":"mean_fmol_chl_POC","format":"","frictionlessType":"","description":"

average ratio of chlorophyll-a to particulate organic carbon

","units":{"_id":"","name":"unitless"},"type":{"_id":"1073","name":"no_bcodmo_term","description":"

An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"521401","name":"stdev_fmol_chl_POC","format":"","frictionlessType":"","description":"

standard deviation of ratio of chlorophyll-a to particulate organic carbon

","units":{"_id":"","name":"unitless"},"type":{"_id":"1974","name":"standard deviation","description":"

Generic standard deviation value, taking on the units of the parameter that it is tied to.

"}},{"_id":"521402","name":"comments","format":"","frictionlessType":"","description":"

comments

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free text comments, may only have meaning to submitting PI

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