http://lod.bco-dmo.org/id/dataset/521670
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2014-08-11
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Larval mussel shell composition under high and low pH and O2 from SIO, UCSD from 2012 and 2013 (OA_Proxies project)
2014-08-07
publication
2014-08-07
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2020-10-01
publication
https://doi.org/10.26008/1912/bco-dmo.521670.1
Lisa A. Levin
University of California-San Diego Scripps
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Levin, L. A. (2020) Larval mussel shell composition under high and low pH and O2 from SIO, UCSD from 2012 and 2013 (OA_Proxies project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2014-08-07 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.521670.1 [access date]
Larval mussel shell composition under high and low pH and O2 Dataset Description: <p>Larval mussel shell composition under high and low pH and O2 from SIO, UCSD from 2012 and 2013.</p> Methods and Sampling: <p>Mussel larvae were obtained and cultured as described in Frieder et al. 2014, EST. At the end of each experiment, the larvae were frozen then cleaned from organic material using trace-metal-clean techniques in a clean room (described in Frieder et al. 2014, EST).</p>
<p>Once clean and mounted, larvae were individually analyzed for a combination of elements (Mg, Cu, Zn, Sr, Ba, Pb, U with respect to Ca) at UC Santa Barbara using a New Wave research UP-213 laser ablation unit coupled with a Thermo Element 2 single-collector inductively coupled plasma mass spectrometer operating in low resolution mode.</p>
<p>As described in the supplemental material for Frieder et al. 2014 (EST) "three standards were used to ensure proper calibration of the instrument: a solution-based dissolved CaCO3 reference material (OTO, [Spex certified primary standard solutions]), and two solid standards, NIST 612 and USGS MACS3 CaCO3 standard. All reference materials, both solution- and solid-based, were analyzed at the beginning and the end of each run; mounting medium[, solution standards] and instrument blanks were run multiple times during a sequence. For ablations, laser settings were a single, 30-µm-long by 80-µm-wide line at 40% power, 10 Hz, and 8 µm s-1 scanning speed. These settings usually consumed the entire larval shell. The tape used to mount the shells had less than 5% of the average shell value for each element."</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1041062 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1041062
completed
Lisa A. Levin
University of California-San Diego Scripps
858-534-3579
9500 Gilman Drive Mail Code: 0218
La Jolla
CA
92093-0218
USA
llevin@ucsd.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
species
sample
replicate
treatment
Mg_Ca
Cu_Ca
Zn_Ca
Sr_Ca
Ba_Ca
Pb_Ca
U_Ca
ICP Mass Spec
theme
None, User defined
species scientific name (binomial)
sample identification
replicate
treatment
ratio
featureType
BCO-DMO Standard Parameters
Inductively Coupled Plasma Mass Spectrometer
instrument
BCO-DMO Standard Instruments
Levin_mussels
service
Deployment Activity
Kaplan Experimental Aquarium at Scripps Institution of Oceanography, UCSD
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA)
https://www.nsf.gov/funding/pgm_summ.jsp?pims_id=503477
Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA)
NSF Climate Research Investment (CRI) activities that were initiated in 2010 are now included under Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES). SEES is a portfolio of activities that highlights NSF's unique role in helping society address the challenge(s) of achieving sustainability. Detailed information about the SEES program is available from NSF (https://www.nsf.gov/funding/pgm_summ.jsp?pims_id=504707).
In recognition of the need for basic research concerning the nature, extent and impact of ocean acidification on oceanic environments in the past, present and future, the goal of the SEES: OA program is to understand (a) the chemistry and physical chemistry of ocean acidification; (b) how ocean acidification interacts with processes at the organismal level; and (c) how the earth system history informs our understanding of the effects of ocean acidification on the present day and future ocean.
Solicitations issued under this program:
NSF 10-530, FY 2010-FY2011
NSF 12-500, FY 2012
NSF 12-600, FY 2013
NSF 13-586, FY 2014
NSF 13-586 was the final solicitation that will be released for this program.
PI Meetings:
1st U.S. Ocean Acidification PI Meeting(March 22-24, 2011, Woods Hole, MA)
2nd U.S. Ocean Acidification PI Meeting(Sept. 18-20, 2013, Washington, DC)
3rd U.S. Ocean Acidification PI Meeting (June 9-11, 2015, Woods Hole, MA – Tentative)
NSF media releases for the Ocean Acidification Program:
Press Release 10-186 NSF Awards Grants to Study Effects of Ocean Acidification
Discovery Blue Mussels "Hang On" Along Rocky Shores: For How Long?
Discovery nsf.gov - National Science Foundation (NSF) Discoveries - Trouble in Paradise: Ocean Acidification This Way Comes - US National Science Foundation (NSF)
Press Release 12-179 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: Finding New Answers Through National Science Foundation Research Grants - US National Science Foundation (NSF)
Press Release 13-102 World Oceans Month Brings Mixed News for Oysters
Press Release 13-108 nsf.gov - National Science Foundation (NSF) News - Natural Underwater Springs Show How Coral Reefs Respond to Ocean Acidification - US National Science Foundation (NSF)
Press Release 13-148 Ocean acidification: Making new discoveries through National Science Foundation research grants
Press Release 13-148 - Video nsf.gov - News - Video - NSF Ocean Sciences Division Director David Conover answers questions about ocean acidification. - US National Science Foundation (NSF)
Press Release 14-010 nsf.gov - National Science Foundation (NSF) News - Palau's coral reefs surprisingly resistant to ocean acidification - US National Science Foundation (NSF)
Press Release 14-116 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: NSF awards $11.4 million in new grants to study effects on marine ecosystems - US National Science Foundation (NSF)
SEES-OA
largerWorkCitation
program
Development of geochemical proxies to evaluate larval pH-exposure history
https://osprey.bco-dmo.org/project/2212
Development of geochemical proxies to evaluate larval pH-exposure history
<p>This research is funded as part of NSF CRI Ocean Acidification Category 2. The investigators will develop a new interdisciplinary partnership between connectivity ecology (Levin at SIO), metal isotope geochemistry (Anbar and Gordon at ASU), and paleoclimatology (Herrmann at ASU/LSU) to identify new proxies for ocean acidification that can be used to assess pH exposures in living organisms and, potentially to interpret the geologic record. The investigators hypothesize that the isotopic composition of larval calcium carbonates reflects changes in seawater chemistry driven by ocean acidification and, in some instances, with associated decline in oxygen levels. The large extent to which these two parameters vary in concert in the modern and past ocean (and thus have joint influence), and the extent to which they may be uncoupled by anthropogenic CO2 inputs, merits considerable attention. Thus, the integration of pH and oxygen in proxy development would be an important advance.</p>
<p>The focus of this project is on proxy development to determine pH exposure history for living organisms in their larval state, and will center on calcium, boron, and uranium isotopes as well as multi-elemental fingerprints. For this project, the investigators will target open coast, front bay and backbay mytilid mussel species, each living naturally under a different pH regime, and statoliths of encapsulated market squid larvae from the open shelf. Larvae with known pH, oxygen and temperature exposure histories will be obtained from (1) laboratory larval rearing experiments that manipulate pH and oxygen and (2) in situ out planting of lab-spawned larvae in larval homes onto existing moorings where pH, T and oxygen are being monitored. Analyses will employ SIMS (for del 11B), multicollector (for del 44Ca, del 238 U), and laser ablation ICP-MS (targeting B, Cu, U, Pb, Mo, and a suite of additional pH- and redox-sensitive trace elements). Multivariate statistical tools will define ability to detect pH-induced signatures and to determine species or taxon-specific vital effects. The investigators are exploring proxies for invertebrate larvae that are untested in the context of acidification geochemistry. Targeting larvae is critical as many marine organisms produce larval carbonate structures and these stages may be most affected by ocean acidification. The retention of larval shell and statoliths after recruitment may ultimately allow us to test the importance of larval pH and O2 exposure to survival and population persistence. An ability to assess past exposures through geochemical proxies will provide information about relative pH tolerances and ecosystem-level change in response to changes in the ocean's carbonate chemistry.</p>
<p><strong>NOTE</strong>: A series of laboratory experiments were run in which <em>Mytilus</em> spp. larvae (<em>Mytilus californianus</em> and <em>Mytilus galloprovincialis</em>) and <em>Doryteuthis opalescens</em> (market squid) embryos were reared under controlled temperature, pH and oxygen conditions. Experimental conditions are given in <a href="http://bcodata.whoi.edu/OA_Proxy_Development/BCO-DMO_Table1_Experimental_Seawater_Conditions_Mussels_v2_2014-08-27.jpg" target="_blank">Table 1</a> (for mussel larvae) and <a href="http://bcodata.whoi.edu/OA_Proxy_Development/Table2_Levin_OCE1041062.pdf" target="_blank">Table 2</a> (for squid embryos). Geochemistry data in the form of Metal:Ca ratios for mussels has been uploaded to BCO-DMO as "Mussel shell trace element ratios" and squid statolith geochemistry data are available on request.</p>
OA_Proxies
largerWorkCitation
project
eng; USA
oceans
Kaplan Experimental Aquarium at Scripps Institution of Oceanography, UCSD
-119.842
-117.203
32.725
34.4128
2012-05-05
2013-01-23
Southern California, 32 N 117 W
0
BCO-DMO catalogue of parameters from Larval mussel shell composition under high and low pH and O2 from SIO, UCSD from 2012 and 2013 (OA_Proxies project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/522868.rdf
Name: species
Units: unitless
Description: <p>experimental organism taxonomic species name</p>
http://lod.bco-dmo.org/id/dataset-parameter/522869.rdf
Name: sample
Units: unitless
Description: <p>sample identification</p>
http://lod.bco-dmo.org/id/dataset-parameter/522870.rdf
Name: replicate
Units: unitless
Description: <p>replicate code</p>
http://lod.bco-dmo.org/id/dataset-parameter/522871.rdf
Name: treatment
Units: unitless
Description: <p>High pH= ; low pH= ; high O2= ; low O2 =</p>
http://lod.bco-dmo.org/id/dataset-parameter/522872.rdf
Name: Mg_Ca
Units: millimoles/mole
Description: <p>Mg:Ca ratio</p>
http://lod.bco-dmo.org/id/dataset-parameter/522873.rdf
Name: Cu_Ca
Units: millimoles/mole
Description: <p>Cu:Ca ratio</p>
http://lod.bco-dmo.org/id/dataset-parameter/522874.rdf
Name: Zn_Ca
Units: millimoles/mole
Description: <p>Zn:Ca ratio</p>
http://lod.bco-dmo.org/id/dataset-parameter/522875.rdf
Name: Sr_Ca
Units: millimoles/mole
Description: <p>Sr:Ca ratio</p>
http://lod.bco-dmo.org/id/dataset-parameter/522876.rdf
Name: Ba_Ca
Units: micromoles/mole
Description: <p>Ba:Ca ratio</p>
http://lod.bco-dmo.org/id/dataset-parameter/522877.rdf
Name: Pb_Ca
Units: micromoles/mole
Description: <p>Pb:Ca ratio</p>
http://lod.bco-dmo.org/id/dataset-parameter/522878.rdf
Name: U_Ca
Units: micromoles/mole
Description: <p>U:Ca ratio</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
72935
https://darchive.mblwhoilibrary.org/bitstream/1912/26264/1/dataset-521670_mussel-shell-trace-element-ratios__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.521670.1
download
onLine
dataset
<p>Mussel larvae were obtained and cultured as described in Frieder et al. 2014, EST. At the end of each experiment, the larvae were frozen then cleaned from organic material using trace-metal-clean techniques in a clean room (described in Frieder et al. 2014, EST).</p>
<p>Once clean and mounted, larvae were individually analyzed for a combination of elements (Mg, Cu, Zn, Sr, Ba, Pb, U with respect to Ca) at UC Santa Barbara using a New Wave research UP-213 laser ablation unit coupled with a Thermo Element 2 single-collector inductively coupled plasma mass spectrometer operating in low resolution mode.</p>
<p>As described in the supplemental material for Frieder et al. 2014 (EST) "three standards were used to ensure proper calibration of the instrument: a solution-based dissolved CaCO3 reference material (OTO, [Spex certified primary standard solutions]), and two solid standards, NIST 612 and USGS MACS3 CaCO3 standard. All reference materials, both solution- and solid-based, were analyzed at the beginning and the end of each run; mounting medium[, solution standards] and instrument blanks were run multiple times during a sequence. For ablations, laser settings were a single, 30-µm-long by 80-µm-wide line at 40% power, 10 Hz, and 8 µm s-1 scanning speed. These settings usually consumed the entire larval shell. The tape used to mount the shells had less than 5% of the average shell value for each element."</p>
Specified by the Principal Investigator(s)
<p>All isotope data were originally measured by the mass spec as intensities (counts per second or cps) relative to cps of 48Ca to control for differences in amount of material analyzed per sample. Detection limits were determined by running blanks where no sample material was being analyzed to measure any background levels of the element ratios. For each sequence run, the cps measurements of background blanks were averaged separately for each elemental ratio. The detection limit for each elemental ratio was set to three standard deviations above the average intensity of the blanks. Any sample measurement above the detection limit was determined to be a legitimate sample reading as it was significantly above the background levels. Any sample measurements falling below the detection limit were not used for further analysis.&nbsp;</p>
<p>For those samples with elemental counts above the detection limit, each isotope ratio intensity was compared to the known concentrations of each element in the OTO solution standard and altered for machine drift (measured by comparing the solution standards run throughout the sequence) to determine the element ratios in units of mmol/mol Ca. These mmol/mol or µmol/mol ratios are what we consider to be the "raw" values presented in this mussel geochemistry dataset. All the listed samples were those used in the various statistical analyses.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
ICP Mass Spec
ICP Mass Spec
PI Supplied Instrument Name: ICP Mass Spec PI Supplied Instrument Description:LA-ICP-MS (Laser Ablation Inductively Coupled Plasma Mass Spectrometry): Thermo Element 2 single-collector
ICP-MS operating in low-resolution mode with a New Wave Research UP-213 laser ablation unit (at the University of
California Santa Barbara). Instrument Name: Inductively Coupled Plasma Mass Spectrometer Instrument Short Name:ICP Mass Spec Instrument Description: An ICP Mass Spec is an instrument that passes nebulized samples into an inductively-coupled gas plasma (8-10000 K) where they are atomized and ionized. Ions of specific mass-to-charge ratios are quantified in a quadrupole mass spectrometer. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB15/
Deployment: Levin_mussels
Levin_mussels
SIO Levin
SIO Levin
laboratory
Levin_mussels
Lisa A. Levin
University of California-San Diego Scripps
SIO Levin
SIO Levin
laboratory