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Mass flow controllers were used to deliver a gas mixture of N2, O2, and CO2 to gas membrane contactors (Liqui-Cel Part No. G501), which in turn delivered the gas mixture to the seawater to maintain the desired CO2 and O2 concentrations in each treatment replicate (Bockmon et al. 2013, Biogeosciences).<\/p>\n
A pH sensor (Honeywell Durafet III) and Aanderaa oxygen optode were used to continuously record from one replicate per treatment, per day. Along with daily discrete samples measured for pH and [O2], this sensor monitoring confirmed the stability of conditions, with the average offset of replicates to be 0.01 pH units and 3\u00b5mol O2 kg-1 (Frieder et al. 2014, GCB). Discrete pH measurements were determined spectrophotometrically and the discrete [O2] values were determined using a Winkler Titration method (as described in Frieder et al. 2014, GCB).<\/p>\n
Discrete samples were taken at the beginning and end of each experiment for the determination of total alkalinity and salinity. Total alkalinity measurements were determined using an open-cell, potentiometric titration with an accuracy of +- 2 \u00b5mol kg-1 (Dickson et al., 2007). Salinity was calculated from density measured at 20\u00b0C on a Density Meter (Mettler Toledo DE45) with an accuracy of 0.05 salinity units. HOBO Pendant temperature data loggers with an accuracy of +-0.5\u00b0C continuously measured temperature every 5 min in all replicates.<\/p><\/div>","@type":"rdf:HTML"}],"http:\/\/ocean-data.org\/schema\/hasBriefDescription":[{"@value":"Experimental seawater chemical properties for larval mussel shell study","@language":"en-US"}],"http:\/\/purl.org\/dc\/terms\/description":[{"@value":"
Experimental seawater chemical properties for larval mussel shell study from SIO, UCSD from 2012 and 2013. This table has been modified from tables in Frieder et al. 2014 GCB and Frieder et al. 2014 ES&T; see those publications for additional corresponding salinity and other carbonate chemistry variables.<\/p><\/div>","@type":"rdf:HTML"}],"http:\/\/www.w3.org\/2000\/01\/rdf-schema#label":[{"@value":"Experimental seawater chemistry","@type":"xsd:string"}],"http:\/\/ocean-data.org\/schema\/hasProcessingDescription":[{"@value":"
BCO-DMO Processing Notes:<\/strong>
\n- data submitted in Excel file \"Levin_BCO-DMO Table 1. Experimental Seawater Conditions - Mussels.xlsx\" sheet \"Seawater conditions\" extracted to tsv
\n- added conventional header with dataset name, PI name, version date
\n- modified parameter names to conform with BCO-DMO naming conventions
\n- removed special characters
\n- replaced \/ with -; replaced space with _
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