<div><p><u>Protein content</u><span style="font-size:13px"> of the larvae was determined spectrophotometrically using the microtiter plate protocol of the Bio-Rad protein assay with Coomassie brilliant blue dye (Bio-Rad, CA). At each sampling, four groups of larvae (40 group-1 in June and 10 group-1 in July) were frozen in liquid nitrogen and stored until processed. Proteins were solubilized by placing the larvae in 0.1 M NaOH, disrupting them with ultrasonic vibrations (Branson Digital Sonifier S-250D, USA), and warming at 50C for 5 h. The protein extract was neutralized with HCl, diluted where necessary, and processed in triplicate with the addition of the dye reagent on a microtiter plate. Following 30-min incubations, absorbances were measured at 595 nm using a plate reader (Biotek Synergy H4 Hybrid Reader, USA) and converted to protein using a calibration prepared from bovine serum albumin. Protein content was expressed as mg larva-1.</span></p>
<p><u>Maximum quantum yield (Fv/Fm) of Symbiodinium within the larvae:</u> To assess the condition and number of Symbiodinium, larvae were processed for maximum photochemical efficiency of PSII (Fv/Fm) and Symbiodinium density. Fv/Fm was measured using pulse amplitude modulation fluorometry (PAM) with a Diving-PAM (Walz, GmbH) fitted with an 8-mm-diameter probe and operated at constant settings for measuring intensity and gain (both set at 10). Four replicates of 8 larvae were dark-adapted for 2 h and transferred under weak red light to the tip of the sensor where they were retained within a drop of water. Previous experiments demonstrated that fluorescent measures could be obtained reliably with C6 larvae. Larvae processed for Fv/Fm were removed from the tip of the sensor and used to evaluate size.</p>
<p><u>Larval size and <em>Symbiodinium </em>densities:</u> To determine the size, larvae were placed individually on a microscope slide in a drop of seawater and photographed using a digital camera fitted to a dissecting microscope. Larval area (mm^2) was measured using ImageJ 1.42q software (Abramoff et al. 2004), and mean larval area (n = 8) from each tank at each time point was used for statistical analysis. Finally, these larvae were analyzed for <em>Symbiodinium </em>densities by preserving 4 replicates of 8 larvae in 10 % formalin, and later macerating them with a Teflon pestle. The Symbiodinium in the slurry were counted using a hemocytometer (4 replicate counts) and the algal population expressed as cells larva-1.</p></div>
Brooded coral larval size, protein content, symbiont (Symbiodinium) densities, maximum photochemical efficiency of PSII (Cumbo, 2012)
<div><p>The physiological development of brooded larvae from the pocilloporid coralsĀ <em>Pocillopora damicornis</em> and <em>Seriatopora caliendrum</em> in southern Taiwan was examined.</p>
<p>These data include larval size, protein content, symbiont (Symbiodinium) densities, maximum photochemical efficiency of PSII from experiments conducted in June and July, 2010.</p>
<p><strong>Related datasets:</strong><br /><a href="http://www.bco-dmo.org/dataset/528781">brooded coral - carbonate chemistry</a><br /><a href="http://www.bco-dmo.org/dataset/528641">brooded coral - release</a><br /><a href="http://www.bco-dmo.org/dataset/528695">brooded coral - respiration</a><br /><a href="http://www.bco-dmo.org/dataset/528728">brooded coral - settlement competency</a><br /><a href="http://www.bco-dmo.org/dataset/528761">brooded coral - size_July</a><br /><a href="http://www.bco-dmo.org/dataset/528661">brooded coral - size_protein_symbionts_photosynth</a></p>
<p>These data are published in Cumbo, VR, Fan TY, Edmunds PJ. (2012) Physiological development of brooded larvae from two pocilloporid corals in Taiwan. Marine Biology 159: 2853-2866. DOI 10.1007/s00227-012-2046-y. See Figs. 1b, 1c, 3b, 3c, 3d.</p>
<p><a href="/objectserver/0a7955dbbece331864832af9ee783248/Cumbo_etal_2012_MarBio_BCODMO.xlsx?url=http%3A%2F%2Fdmoserv3.whoi.edu%2Fdata_docs%2FClimate_Coral_Larvae%2FCumbo_etal_2012_MarBio_BCODMO.xlsx&f=6363313338303632656535326537643166303033306461663264393030353462687474703a2f2f646d6f73657276332e77686f692e6564752f646174615f646f63732f436c696d6174655f436f72616c5f4c61727661652f43756d626f5f6574616c5f323031325f4d617242696f5f42434f444d4f2e786c7378" target="_blank">Download complete data for this publication (Excel file)</a></p></div>
brooded coral larvae 1 - size_protein_symbionts_photosynth
<div><p><strong>BCO-DMO processing notes:</strong></p>
<p>- added conventional header with dataset name, PI name, version date, reference information<br />
- added lab, lat, lon, expt columns<br />
- renamed parameters to BCO-DMO standard<br />
- combined data from Figures 1b, 1c, 3b, 3c, 3d</p></div>
528661
brooded coral larvae 1 - size_protein_symbionts_photosynth
2014-09-23T14:41:21-04:00
2014-09-23T14:41:21-04:00
2023-07-07T16:10:26-04:00
urn:bcodmo:dataset:528661
Brooded coral larval size, protein content, symbiont (Symbiodinium) densities, maximum photochemical efficiency of PSII (Cumbo, 2012) from Taiwan, 2010 (Cumbo, 2012) (MCR LTER project, Climate_Coral_Larvae project)
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Edmunds, P. J., Cumbo, V. R. (2014) Brooded coral larval size, protein content, symbiont (Symbiodinium) densities, maximum photochemical efficiency of PSII (Cumbo, 2012) from Taiwan, 2010 (Cumbo, 2012) (MCR LTER project, Climate_Coral_Larvae project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2014-09-08) Version Date 2014-09-08 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/528661 [access date]
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2014-09-08
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2014-09-08
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