http://lod.bco-dmo.org/id/dataset/528834
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2014-09-26
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Respiration rates, photosynthetic efficiency, and mortality of brooded coral larval experiments, March 2011 and 2012, Taiwan (Cumbo, 2013) (MCR LTER project, Climate_Coral_Larvae project)
2014-08-30
publication
2014-08-30
revision
BCO-DMO Linked Data URI
2014-08-30
creation
http://lod.bco-dmo.org/id/dataset/528834
Peter J. Edmunds
California State University Northridge
principalInvestigator
Dr Vivian R Cumbo
California State University Northridge
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Edmunds, P. J., Cumbo, V. R. (2014) Respiration rates, photosynthetic efficiency, and mortality of brooded coral larval experiments, March 2011 and 2012, Taiwan (Cumbo, 2013) (MCR LTER project, Climate_Coral_Larvae project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2014-08-30) Version Date 2014-08-30 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/528834 [access date]
Respiration rates, photosynthetic efficiency, and mortality of brooded coral larval experiments, March 2011 & 2012 (Cumbo, 2013) Dataset Description: <p>To evaluate the effects of temperature and pCO2 on coral larvae, brooded larvae of Pocillopora damicornis from Nanwan Bay, Taiwan (21°56.179' N, 120°44.85' E), were exposed to ambient (419-470 µatm) and high (604-742 µatm) pCO2 at ~25 and ~29 °C in two experiments conducted in March 2010 and March 2012. Larvae were sampled from four consecutive lunar days (LD) synchronized with spawning following the new moon, incubated in treatments for 24 h, and measured for respiration, maximum photochemical efficiency of PSII (F v/F m), and mortality.</p>
<p>The most striking outcome was a strong effect of time (i.e., LD) on larvae performance: respiration was affected by an LD × temperature interaction in 2010 and 2012, as well as an LD × pCO2 × temperature interaction in 2012; F v/F m was affected by LD in 2010 (but not 2012); and mortality was affected by an LD × pCO2 interaction in 2010, and an LD × temperature interaction in 2012. There were no main effects of pCO2 in 2010, but in 2012, high pCO2 depressed metabolic rate and reduced mortality. Therefore, differences in larval performance depended on day of release and resulted in varying susceptibility to future predicted environmental conditions. These results underscore the importance of considering larval brood variation across days when designing experiments. Subtle differences in experimental outcomes between years suggest that transgenerational plasticity in combination with unique histories of exposure to physical conditions can modulate the response of brooded coral larvae to climate change and ocean acidification.</p>
<p>These data include dark respiration rates, maximum photochemical efficiency of PSII (F v/F m), and mortality&nbsp;from the experimental tanks, March 2011 and 2012.</p>
<p><strong>Related datasets:</strong></p>
<p><a href="http://www.bco-dmo.org/dataset/528806">brooded coral larvae 2 - carbonate chemistry</a><br />
<a href="http://www.bco-dmo.org/dataset/528880">brooded coral larvae 2 - larval release March 2003-2008</a></p>
<p>These data are published in Vivian R Cumbo, Peter J Edmunds, Christopher B Wall, Tung-Yung Fan. (2013) Brooded coral larvae differ in their response to high temperature and elevated pCO2 depending on the day of release. Marine Biology. See Figures 1 and 2.</p>
<p><a href="http://dmoserv3.whoi.edu/data_docs/Climate_Coral_Larvae/Cumbo_etal_2013_MarBio_BCODMO.xlsx" target="_blank">Download complete data for this publication (Excel file)</a><br />
Data also available from PANGAEA: <a href="http://doi.pangaea.de/10.1594/PANGAEA.831612" target="_blank">DOI 10.1007/s00227-013-2280-y</a></p> Methods and Sampling: <p>March 2010:<br />
A Ruthenium-based optode (FOXY-R, 1.58 diameter, Ocean Optics) connected to a spectrophotometer (USB2000, Ocean Optics) and interfaced with a computer running Ocean Optics software (OOISensor, version 1.00.08) was used to measure the respiration of the larvae. The optode was 2-point calibrated using a zero solution (0.01 M Na2B4O7*10H2O saturated with Na2SO3) and 100% air saturation using water-saturated air at the treatment temperature. To measure respiration, 6 larvae were removed from the treatment containers and placed into 2-mL glass Wheaton vials filled with filtered seawater from the same treatment tank and sealed with Parafilm. A study conducted concurrently with the present analysis demonstrated that respiration of P. damicornis larvae in identical vials could be measured accurately with 5 larvae in each vial (Edmunds et al. 2011). Respiration measurements were completed after the 24-h incubation period. Larvae were dark-adapted prior to measurements so that respiration would not be stimulated by light (Edmunds and Davies 1988). Initial O2 concentration in the seawater filling the vials was determined before the vials were sealed, and vials without larvae were used as controls. Larvae in the sealed vials were incubated at their temperature treatments for 1.5-2 h in the dark using water baths (±0.1C, Hipoint, models LC-06 and LC-10). Incubation times were selected to ensure that O2 concentrations remained[75%. On completion of the incubations, vials were removed from the chillers, gently inverted to mix the seawater, and analyzed for O2 saturation. O2 saturation was converted to concentration using gas tables [N. Ramsing and J. Gundersen at http://www.unisense.com (based on Garcia and Gordon 1992)] and the temperature and salinity of the seawater, and the change in O2 concentration converted to nmol O2 min-1 larvae-1, after adjusting for control O2 fluxes.</p>
<p>Larval photophysiology was assessed using pulse amplitude modulated (PAM) fluorometry to measure the maximum photochemical efficiency of open reaction centers of photosystem II (RCIIs) following a period of dark adaptation (i.e., Fv/Fm) of their Symbiodinium. Changes in Fv/Fm can detect damage to the photosynthetic apparatus, with declines under elevated temperature indicating damage to PSII (Jones et al. 1998; Bhagooli and Hidaka 2003). These measurements were conducted after the 24-h incubation period, with larvae being dark-adapted during the final 2 h of the incubation. After this period of darkness, Fv/Fm was measured using a diving PAM (Walz, GmbH) fitted with an 8-mm diameter probe and standardized for measuring intensity (setting: 10) and gain (setting: 10). Fluorescence was measured by loading 8 larvae into a drop of seawater on the tip of the probe, with these manipulations completed under weak red illumination. Two groups of 8 larvae were measured for Fv/Fm from each tank, and the average value in each tank was used for statistical analysis.</p>
<p>To assess the number of larvae dying in the treatments, at the conclusion of the incubations, tubs were removed from the treatments and the number of swimming larvae and settled recruits (with tissue) recorded. Due to the rapid breakdown of dead larvae (Yakovleva et al. 2009), larvae that could not be accounted for were assumed dead. Mortality was expressed as a percentage of the number of larvae added at the start of the experiment.</p>
<p>March 2012:<br />
The second experiment was designed to be virtually identical to the first (n = 8 tanks, 2 tanks treatment-1), although the volume of the incubation tanks was increased to 120 L, and the sample size (number of replicate tubs containing larvae in each tank) was doubled with the objective of increasing statistical power and testing for tank effects for the dependent biological variables. Experimental difficulties affecting a single tank made it problematic to include the tank in the statistical analyses, and therefore, replicates were pooled between tanks in each treatment combination. Larvae were sampled from each replicate tub, and their response to the treatment conditions was assessed using dark respiration, photophysiology (Fv/Fm), and mortality, and each dependent variable was measured as described above.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0844785 Award URL: http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=0844785
completed
Peter J. Edmunds
California State University Northridge
818-677-2502
Department of Biology 18111 Nordhoff Street
Northridge
CA
91330-8303
USA
peter.edmunds@csun.edu
pointOfContact
Dr Vivian R Cumbo
California State University Northridge
Dept. of Biology 18111 Nordhoff Street
Northridge,
CA
91330-8303
pointOfContact
asNeeded
Dataset Version: 2014-08-30
Unknown
lab
lat
lon
date
date_new_moon
days
days_after_NM
temp
tank
replicate
respiration
Fv_Fm
mortality
treatment_pCO2
in-situ incubator
spectrophotometer
optode
theme
None, User defined
laboratory
latitude
longitude
date
days
water temperature
tank
replicate
oxygen respiration rates from in situ incubation
max photosynthesis
No BCO-DMO term
Partial pressure of CO2
featureType
BCO-DMO Standard Parameters
In-situ incubator
Spectrophotometer
Optode
instrument
BCO-DMO Standard Instruments
lab_Edmunds_NMMBA
service
Deployment Activity
National Museum of Marine Biology and Aquarium, Taiwan
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Long Term Ecological Research network
http://www.lternet.edu/
Long Term Ecological Research network
adapted from http://www.lternet.edu/
The National Science Foundation established the LTER program in 1980 to support research on long-term ecological phenomena in the United States. The Long Term Ecological Research (LTER) Network is a collaborative effort involving more than 1800 scientists and students investigating ecological processes over long temporal and broad spatial scales. The LTER Network promotes synthesis and comparative research across sites and ecosystems and among other related national and international research programs. The LTER research sites represent diverse ecosystems with emphasis on different research themes, and cross-site communication, network publications, and research-planning activities are coordinated through the LTER Network Office.
2017 LTER research site map obtained from https://lternet.edu/site/lter-network/
LTER
largerWorkCitation
program
Moorea Coral Reef Long-Term Ecological Research site
http://mcr.lternet.edu/
Moorea Coral Reef Long-Term Ecological Research site
<p><strong>From <a href="http://www.lternet.edu/sites/mcr/">http://www.lternet.edu/sites/mcr/</a> and <a href="http://mcr.lternet.edu/">http://mcr.lternet.edu/</a></strong>:<br />
The Moorea Coral Reef LTER site encompasses the coral reef complex that surrounds the island of Moorea, French Polynesia (17°30'S, 149°50'W). Moorea is a small, triangular volcanic island 20 km west of Tahiti in the Society Islands of French Polynesia. An offshore barrier reef forms a system of shallow (mean depth ~ 5-7 m), narrow (~0.8-1.5 km wide) lagoons around the 60 km perimeter of Moorea. All major coral reef types (e.g., fringing reef, lagoon patch reefs, back reef, barrier reef and fore reef) are present and accessible by small boat.</p>
<p>The MCR LTER was established in 2004 by the US National Science Foundation (NSF) and is a partnership between the University of California Santa Barbara and California State University, Northridge. MCR researchers include marine scientists from the UC Santa Barbara, CSU Northridge, UC Davis, UC Santa Cruz, UC San Diego, CSU San Marcos, Duke University and the University of Hawaii. Field operations are conducted from the UC Berkeley Richard B. Gump South Pacific Research Station on the island of Moorea, French Polynesia.</p>
<p><strong>MCR LTER Data:</strong> The Moorea Coral Reef (MCR) LTER data are managed by and available directly from the MCR project data site URL shown above. The datasets listed below were collected at or near the MCR LTER sampling locations, and funded by NSF OCE as ancillary projects related to the MCR LTER core research themes.</p>
<p><strong>This project is supported by continuing grants with slight name variations:</strong><br />
LTER: Long-Term Dynamics of a Coral Reef Ecosystem<br />
LTER: MCR II - Long-Term Dynamics of a Coral Reef Ecosystem<br />
LTER: MCR IIB: Long-Term Dynamics of a Coral Reef Ecosystem<br />
LTER: MCR III: Long-Term Dynamics of a Coral Reef Ecosystem<br />
LTER: MCR IV: Long-Term Dynamics of a Coral Reef Ecosystem</p>
MCR LTER
largerWorkCitation
project
The ecophysiological basis of the response of coral larvae and early life history stages to global climate change
https://www.bco-dmo.org/project/2235
The ecophysiological basis of the response of coral larvae and early life history stages to global climate change
<p><span style="font-size:13px">Tropical coral reefs face a suite of environmental assaults ranging from anchor damage to the effects of global climate change (GCC). The consequences are evident throughout the tropics, where many coral reefs have lost a substantial fraction of their coral cover in a few decades. Notwithstanding the importance of reducing the impacts of environmental stresses, the only means by which these ecosystems can recover (or simply persist) is through the recruitment of scleractinians, which is a function of successful larval development, delivery, settlement, metamorphosis, and post-settlement events. Despite wide recognition of the importance of these processes, there are few pertinent empirical data, and virtually none that address the mechanisms mediating the success of early coral life stages in a physical environmental varying at multiple spatio-temporal scales.</span></p>
<p>The objective of this research is to complete one of the first comprehensive ecophysiological analyses of the early life stages of corals through a description of: (1) their functionality under 'normal' conditions, and (2) their response to the main drivers of GCC. These analyses will be completed for 2 species representative of a brooding life history strategy, and the experiments will be completed in two locations, one (Taiwan) that provides unrivalled experience in coral reproductive biology, and superb microcosm facilities, and the other (Moorea), with access to a relatively pristine environment, a well described ecological and oceanographic context (through the MCR-LTER), and the capacity to bring a strong biogeographic contrast to the project. The results of the study will be integrated through modeling to explore the effects of GCC on coral community structure over the next century.</p>
<p><strong>The following publications and data resulted from this project:</strong></p>
<p>2013 Wall CB, Fan TY, Edmunds PJ. Ocean acidification has no effect on thermal bleaching in the coral <em>Seriatopora caliendrum</em>. Coral Reefs 33: 119-130.<br /><a href="http://www.bco-dmo.org/dataset/522591" target="_blank">Symbiodinium_Seriatopora photosynthesis</a><br /><a href="http://www.bco-dmo.org/dataset/522737" target="_blank">Symbiodinium_Seriatopora PI curve</a><br /><a href="http://www.bco-dmo.org/dataset/522697" target="_blank">Symbiodinium_Seriatopora temp-salinity-light</a><br /><a href="http://www.bco-dmo.org/dataset/522631" target="_blank">Symbiodinium_Seriatopora water chemistry</a><br /><a href="/objectserver/cc5210ddb2c9e3c3151c1c4e4d7c8b31/Wall_Fan_Edmunds_for_Taiwan_Bleaching_data2013_BCODMO.xlsx?url=http%3A%2F%2Fdmoserv3.whoi.edu%2Fdata_docs%2FClimate_Coral_Larvae%2FWall_Fan_Edmunds_for_Taiwan_Bleaching_data2013_BCODMO.xlsx&f=3765373630333966356136383763313937336535373161373037663635313430687474703a2f2f646d6f73657276332e77686f692e6564752f646174615f646f63732f436c696d6174655f436f72616c5f4c61727661652f57616c6c5f46616e5f45646d756e64735f666f725f54616977616e5f426c65616368696e675f64617461323031335f42434f444d4f2e786c7378" target="_blank">- Download complete data for this publication (Excel file)</a></p>
<p>2013 Wall CB, Edmunds PJ. <em>In situ </em>effects of low pH and elevated HCO3- on juvenile <em>Porites</em> spp. in Moorea, French Polynesia. Biological Bulletin 225:92-101.<br />
Data at <a href="http://mcr.lternet.edu/" target="_blank">MCR</a> and PANGEA: <a href="/objectserver/e848033c8cdb7a63afd532967815e140/PANGAEA.833913?url=http%3A%2F%2Fdoi.pangaea.de%2F10.1594%2FPANGAEA.833913&f=3839323035303635626632653138353732323030656139643739616437313235687474703a2f2f646f692e70616e676165612e64652f31302e313539342f50414e474145412e383333393133" target="_blank">doi.pangaea.de/10.1594/PANGAEA.833913</a><br /><a href="/objectserver/01009bc0d4a486f43397005b913157fe/Wall_Edmunds_2013_BioBull_BCODMO.xlsx?url=http%3A%2F%2Fdmoserv3.whoi.edu%2Fdata_docs%2FClimate_Coral_Larvae%2FWall_Edmunds_2013_BioBull_BCODMO.xlsx&f=6536613461626239633131643038653266306264306162343261363338633430687474703a2f2f646d6f73657276332e77686f692e6564752f646174615f646f63732f436c696d6174655f436f72616c5f4c61727661652f57616c6c5f45646d756e64735f323031335f42696f42756c6c5f42434f444d4f2e786c7378" target="_blank">- Download complete data for this publication (Excel file)</a></p>
<p>2013 Vivian R Cumbo, Peter J Edmunds, Christopher B Wall, Tung-Yung Fan. Brooded coral larvae differ in their response to high temperature and elevated pCO2 depending on the day of release. Marine Biology DOI 10.1007/s00227-013-2280-y.<br />
Data also at PANGEA: <a href="/objectserver/2c4577ae7cd80c435340335739d6b2ef/PANGAEA.831612?url=http%3A%2F%2Fdoi.pangaea.de%2F10.1594%2FPANGAEA.831612&f=3761313236316266386566613631336430336236313938646361363034353865687474703a2f2f646f692e70616e676165612e64652f31302e313539342f50414e474145412e383331363132" target="_blank">doi.pangaea.de/10.1594/PANGAEA.831612</a><br /><a href="http://www.bco-dmo.org/dataset/528806">brooded coral larvae 2 - carbonate chemistry</a><br /><a href="http://www.bco-dmo.org/dataset/528880">brooded coral larvae 2 - larval release March 2003-2008</a><br /><a href="http://www.bco-dmo.org/dataset/528834">brooded coral larvae 2 - respiration_photosyth_mortality</a><br /><a href="/objectserver/cbd4d6d71585ed335099a828622c2080/Cumbo_etal_2013_MarBio_BCODMO.xlsx?url=http%3A%2F%2Fdmoserv3.whoi.edu%2Fdata_docs%2FClimate_Coral_Larvae%2FCumbo_etal_2013_MarBio_BCODMO.xlsx&f=3335623365346632653135326437363031663238626365616565323635376633687474703a2f2f646d6f73657276332e77686f692e6564752f646174615f646f63732f436c696d6174655f436f72616c5f4c61727661652f43756d626f5f6574616c5f323031335f4d617242696f5f42434f444d4f2e786c7378" target="_blank">- Download complete data for this publication (Excel file)</a></p>
<p>2013 Edmunds PJ, Cumbo VR, Fan TY. Metabolic costs of larval settlement and metamorphosis in the coral <em>Seriatopora caliendrum</em> under ambient and elevated pCO2. Journal Experimental Marine Biology and Ecology 443: 33-38 Data also at PANGEA: <a href="/objectserver/70427056c68798f8ce59d6859860bb63/PANGAEA.821644?url=http%3A%2F%2Fdoi.pangaea.de%2F10.1594%2FPANGAEA.821644&f=3038326366653330396137323161646332356565373965663438383433386432687474703a2f2f646f692e70616e676165612e64652f31302e313539342f50414e474145412e383231363434" target="_blank">doi:10.1594/PANGAEA.821644</a><br /><a href="http://www.bco-dmo.org/dataset/526674" target="_blank">Coral post-settlement physiology</a><br /><a href="/objectserver/11c2229d7e7b63f1dd331f36c8f9dee7/Edmunds_etal_2013_JEMBE443_BCODMO.xls?url=http%3A%2F%2Fdmoserv3.whoi.edu%2Fdata_docs%2FClimate_Coral_Larvae%2FEdmunds_etal_2013_JEMBE443_BCODMO.xls&f=6664643335383633626139393237643435366565636536643566323661336663687474703a2f2f646d6f73657276332e77686f692e6564752f646174615f646f63732f436c696d6174655f436f72616c5f4c61727661652f45646d756e64735f6574616c5f323031335f4a454d42453434335f42434f444d4f2e786c73" target="_blank">- Download complete data for this publication (Excel file)</a></p>
<p>2013 Aaron M Dufault, Aaron Ninokawa, Lorenzo Bramanti, Vivian R Cumbo, Tung-Yung Fan, Peter J Edmunds. The role of light in mediating the effects of ocean acidification on coral calcification. Journal of Experimental Biology 216: 1570-1577.<br /><a href="http://www.bco-dmo.org/dataset/527500">coral-light expt.- PAR</a><br /><a href="http://www.bco-dmo.org/dataset/527752">coral-light expt.- carbonate chemistry</a><br /><a href="http://www.bco-dmo.org/dataset/527729">coral-light expt.- temp_salinity</a><br /><a href="http://www.bco-dmo.org/dataset/527816">coral-light expt.- growth</a><br /><a href="http://www.bco-dmo.org/dataset/527782">coral-light expt.- protein</a><br /><a href="http://www.bco-dmo.org/dataset/527851">coral-light expt.- survival</a><br /><a href="/objectserver/5c8eae1f808d809c0e8379e1c517dd98/Dufault_etal_2013_JEB_BCODMO.xlsx?url=http%3A%2F%2Fdmoserv3.whoi.edu%2Fdata_docs%2FClimate_Coral_Larvae%2FDufault_etal_2013_JEB_BCODMO.xlsx&f=3430343338396334383239656262323030383062643761363564643038386536687474703a2f2f646d6f73657276332e77686f692e6564752f646174615f646f63732f436c696d6174655f436f72616c5f4c61727661652f44756661756c745f6574616c5f323031335f4a45425f42434f444d4f2e786c7378" target="_blank">- Download complete data for this publication (Excel file)</a></p>
<p>2012 Cumbo, VR, Fan TY, Edmunds PJ. Effects of exposure duration on the response of <em>Pocillopora damicornis</em> larvae to elevated temperature and high pCO2. J Exp Mar Biol Ecol 439: 100-107.<br />
Data is also at PANGEA: <a href="/objectserver/9e9e97cedbe2c2952a202b25bedc36e2/PANGAEA.823582?url=http%3A%2F%2Fdoi.pangaea.de%2F10.1594%2FPANGAEA.823582&f=3230643038386163326631326163303637326335363837353761623762653365687474703a2f2f646f692e70616e676165612e64652f31302e313539342f50414e474145412e383233353832" target="_blank">doi:10.1594/PANGAEA.823582</a><br /><a href="http://www.bco-dmo.org/dataset/535163">brooded coral larvae 3 - carbonate chemistry</a><br /><a href="http://www.bco-dmo.org/dataset/535219">brooded coral larvae 3 - light</a><br /><a href="http://www.bco-dmo.org/dataset/535462">brooded coral larvae 3 - mortality</a><br /><a href="http://www.bco-dmo.org/dataset/535425">brooded coral larvae 3 - protein</a><br /><a href="http://www.bco-dmo.org/dataset/535328">brooded coral larvae 3 - respiration and protein</a><br /><a href="http://www.bco-dmo.org/dataset/535266">brooded coral larvae 3 - respiration raw data</a><br /><a href="http://www.bco-dmo.org/dataset/535358">brooded coral larvae 3 - symbiont density</a><br /><a href="http://www.bco-dmo.org/dataset/535244">brooded coral larvae 3 - tank temperature</a><br /><a href="/objectserver/fca3d0d875d0dde316adaf99fd343fb3/Cumbo_etal_2012_JEMBE_data1_BCODMO.xls?url=http%3A%2F%2Fdmoserv3.whoi.edu%2Fdata_docs%2FClimate_Coral_Larvae%2FCumbo_etal_2012_JEMBE_data1_BCODMO.xls&f=3531353531383633643631653932316130323336643964383734346331363139687474703a2f2f646d6f73657276332e77686f692e6564752f646174615f646f63732f436c696d6174655f436f72616c5f4c61727661652f43756d626f5f6574616c5f323031325f4a454d42455f64617461315f42434f444d4f2e786c73" target="_blank">- Download part 1 of data for this publication (Excel file)</a><br /><a href="/objectserver/c92fd25dc854468e957a70344e2af995/Cumbo_etal_2012_JEMBE_Tank Parameters_BCODMO.xlsx?url=http%3A%2F%2Fdmoserv3.whoi.edu%2Fdata_docs%2FClimate_Coral_Larvae%2FCumbo_etal_2012_JEMBE_Tank+Parameters_BCODMO.xlsx&f=3063333034366338396136616635356136636164303432323335376336333131687474703a2f2f646d6f73657276332e77686f692e6564752f646174615f646f63732f436c696d6174655f436f72616c5f4c61727661652f43756d626f5f6574616c5f323031325f4a454d42455f54616e6b20506172616d65746572735f42434f444d4f2e786c7378" target="_blank">- Download tank parameters data for this publication (Excel file)</a></p>
<p>2012 Cumbo, VR, Fan TY, Edmunds PJ. Physiological development of brooded larvae from two pocilloporid corals in Taiwan. Marine Biology 159: 2853-2866.<br /><a href="http://www.bco-dmo.org/dataset/528781">brooded coral - carbonate chemistry</a><br /><a href="http://www.bco-dmo.org/dataset/528641">brooded coral - release</a><br /><a href="http://www.bco-dmo.org/dataset/528695">brooded coral - respiration</a><br /><a href="http://www.bco-dmo.org/dataset/528728">brooded coral - settlement competency</a><br /><a href="http://www.bco-dmo.org/dataset/528761">brooded coral - size_July</a><br /><a href="http://www.bco-dmo.org/dataset/528661">brooded coral - size_protein_symbionts_photosynth</a><br /><a href="/objectserver/84e00d3bf27f9f075afa32e4c9fc6acd/Cumbo_etal_2012_MarBio_BCODMO .xlsx?url=http%3A%2F%2Fdmoserv3.whoi.edu%2Fdata_docs%2FClimate_Coral_Larvae%2FCumbo_etal_2012_MarBio_BCODMO+.xlsx&f=3961306239626463663964656432383336363136323134346663623032623834687474703a2f2f646d6f73657276332e77686f692e6564752f646174615f646f63732f436c696d6174655f436f72616c5f4c61727661652f43756d626f5f6574616c5f323031325f4d617242696f5f42434f444d4f202e786c7378" target="_blank">- Download complete data for this publication (Excel file)</a></p>
<p>2012 Dufault, Aaron M; Vivian R Cumbo; Tung-Yung Fan; Peter J Edmunds. Effects of diurnally oscillating pCO2 on the calcification and survival of coral recruits. Royal Society of London (B) 279: 2951-2958. doi:10.1098/rspb.2011.2545<br />
Data is also at PANGEA: <a href="/objectserver/6626b84bc62b12efe32e464b1fa52630/PANGAEA.830185?url=http%3A%2F%2Fdoi.pangaea.de%2F10.1594%2FPANGAEA.830185&f=6134363433663936306362626232643336643731346338316163333831333164687474703a2f2f646f692e70616e676165612e64652f31302e313539342f50414e474145412e383330313835" target="_blank">doi:10.1594/PANGAEA.830185</a><br /><a href="http://www.bco-dmo.org/dataset/514198" target="_blank">recruit_growth_area</a><br /><a href="http://www.bco-dmo.org/dataset/506245" target="_blank">recruit_growth_weight</a><br /><a href="http://www.bco-dmo.org/dataset/516783" target="_blank">recruit_seawater_chemistry</a><br /><a href="http://www.bco-dmo.org/dataset/516529" target="_blank">recruit_survival</a><br /><a href="/objectserver/e2119d3219abb109ae7639886772639b/Dufault_etal_2012_RoyProcB_BCODMO.xlsx?url=http%3A%2F%2Fdmoserv3.whoi.edu%2Fdata_docs%2FClimate_Coral_Larvae%2FDufault_etal_2012_RoyProcB_BCODMO.xlsx&f=6434306561646535363133616535316438613535316466376532313437336261687474703a2f2f646d6f73657276332e77686f692e6564752f646174615f646f63732f436c696d6174655f436f72616c5f4c61727661652f44756661756c745f6574616c5f323031325f526f7950726f63425f42434f444d4f2e786c7378" target="_blank">- Download complete data for this publication (Excel file)</a></p>
<p>2011 Edmunds PJ, Cumbo V, Fan TY. Effects of temperature on the respiration of brooded larvae from tropical reef corals. Journal of Experimental Biology 214: 2783-2790. <br /><a href="http://www.bco-dmo.org/dataset/3790" target="_blank">CoralLarvae_comparison_respir</a><br /><a href="http://www.bco-dmo.org/dataset/3809" target="_blank">CoralLarvae_release</a><br /><a href="http://www.bco-dmo.org/dataset/3802" target="_blank">CoralLarvae_respir</a><br /><a href="http://www.bco-dmo.org/dataset/3795" target="_blank">CoralLarvae_size</a><br /><a href="/objectserver/bc178b3fbf1b2188f55a6d860bba9140/Edmunds_etal_2011_JEB_BCODMO.xls?url=http%3A%2F%2Fdmoserv3.whoi.edu%2Fdata_docs%2FClimate_Coral_Larvae%2FEdmunds_etal_2011_JEB_BCODMO.xls&f=3036373638373833366661643130303966343365363830323562366231666632687474703a2f2f646d6f73657276332e77686f692e6564752f646174615f646f63732f436c696d6174655f436f72616c5f4c61727661652f45646d756e64735f6574616c5f323031315f4a45425f42434f444d4f2e786c73" target="_blank">- Download complete data for this publication (Excel file)</a></p>
Climate_Coral_Larvae
largerWorkCitation
project
eng; USA
oceans
National Museum of Marine Biology and Aquarium, Taiwan
2014-08-30
From projects that focused on the following 2 locations: 1. Island of Moorea, French Polynesia 2. Moorea, French Polynesia; Southern Taiwan; California State University Northridge
0
BCO-DMO catalogue of parameters from Respiration rates, photosynthetic efficiency, and mortality of brooded coral larval experiments, March 2011 and 2012, Taiwan (Cumbo, 2013) (MCR LTER project, Climate_Coral_Larvae project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/528865.rdf
Name: lab
Units: unitless
Description: laboratory
http://lod.bco-dmo.org/id/dataset-parameter/528866.rdf
Name: lat
Units: decimal degrees
Description: latitude; north is positive
http://lod.bco-dmo.org/id/dataset-parameter/528867.rdf
Name: lon
Units: decimal degrees
Description: longitude; east is positive
http://lod.bco-dmo.org/id/dataset-parameter/528868.rdf
Name: date
Units: yyyy-mm-dd
Description: local date
http://lod.bco-dmo.org/id/dataset-parameter/528869.rdf
Name: date_new_moon
Units: yyyy-mm-dd
Description: date of the new moon
http://lod.bco-dmo.org/id/dataset-parameter/528870.rdf
Name: days
Units: unitless
Description: days since start of experiment
http://lod.bco-dmo.org/id/dataset-parameter/528871.rdf
Name: days_after_NM
Units: unitless
Description: days since the new moon
http://lod.bco-dmo.org/id/dataset-parameter/528872.rdf
Name: temp
Units: degrees Celsius
Description: target temperature
http://lod.bco-dmo.org/id/dataset-parameter/528873.rdf
Name: tank
Units: tank
Description: tank id number
http://lod.bco-dmo.org/id/dataset-parameter/528874.rdf
Name: replicate
Units: unitless
Description: replicate number
http://lod.bco-dmo.org/id/dataset-parameter/528875.rdf
Name: respiration
Units: nmol/Larvae/min
Description: respiration rate
http://lod.bco-dmo.org/id/dataset-parameter/528876.rdf
Name: Fv_Fm
Units: unitless
Description: maximum photochemical quantum yield of photosystem II
http://lod.bco-dmo.org/id/dataset-parameter/528877.rdf
Name: mortality
Units: percent
Description: percent of presumed dead larvae
http://lod.bco-dmo.org/id/dataset-parameter/528878.rdf
Name: treatment_pCO2
Units: unitless
Description: relative pCO2: ambient=419-470 uatm; high=604-742 uatm
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
11981
https://datadocs.bco-dmo.org/file/1VVMo0PIp4OGBW/brood2_respir_photosyn_mort.csv
brood2_respir_photosyn_mort.csv
Primary data file for dataset ID 528834
download
https://www.bco-dmo.org/dataset/528834/data/download
download
onLine
dataset
<p>March 2010:<br />
A Ruthenium-based optode (FOXY-R, 1.58 diameter, Ocean Optics) connected to a spectrophotometer (USB2000, Ocean Optics) and interfaced with a computer running Ocean Optics software (OOISensor, version 1.00.08) was used to measure the respiration of the larvae. The optode was 2-point calibrated using a zero solution (0.01 M Na2B4O7*10H2O saturated with Na2SO3) and 100% air saturation using water-saturated air at the treatment temperature. To measure respiration, 6 larvae were removed from the treatment containers and placed into 2-mL glass Wheaton vials filled with filtered seawater from the same treatment tank and sealed with Parafilm. A study conducted concurrently with the present analysis demonstrated that respiration of P. damicornis larvae in identical vials could be measured accurately with 5 larvae in each vial (Edmunds et al. 2011). Respiration measurements were completed after the 24-h incubation period. Larvae were dark-adapted prior to measurements so that respiration would not be stimulated by light (Edmunds and Davies 1988). Initial O2 concentration in the seawater filling the vials was determined before the vials were sealed, and vials without larvae were used as controls. Larvae in the sealed vials were incubated at their temperature treatments for 1.5-2 h in the dark using water baths (±0.1C, Hipoint, models LC-06 and LC-10). Incubation times were selected to ensure that O2 concentrations remained[75%. On completion of the incubations, vials were removed from the chillers, gently inverted to mix the seawater, and analyzed for O2 saturation. O2 saturation was converted to concentration using gas tables [N. Ramsing and J. Gundersen at http://www.unisense.com (based on Garcia and Gordon 1992)] and the temperature and salinity of the seawater, and the change in O2 concentration converted to nmol O2 min-1 larvae-1, after adjusting for control O2 fluxes.</p>
<p>Larval photophysiology was assessed using pulse amplitude modulated (PAM) fluorometry to measure the maximum photochemical efficiency of open reaction centers of photosystem II (RCIIs) following a period of dark adaptation (i.e., Fv/Fm) of their Symbiodinium. Changes in Fv/Fm can detect damage to the photosynthetic apparatus, with declines under elevated temperature indicating damage to PSII (Jones et al. 1998; Bhagooli and Hidaka 2003). These measurements were conducted after the 24-h incubation period, with larvae being dark-adapted during the final 2 h of the incubation. After this period of darkness, Fv/Fm was measured using a diving PAM (Walz, GmbH) fitted with an 8-mm diameter probe and standardized for measuring intensity (setting: 10) and gain (setting: 10). Fluorescence was measured by loading 8 larvae into a drop of seawater on the tip of the probe, with these manipulations completed under weak red illumination. Two groups of 8 larvae were measured for Fv/Fm from each tank, and the average value in each tank was used for statistical analysis.</p>
<p>To assess the number of larvae dying in the treatments, at the conclusion of the incubations, tubs were removed from the treatments and the number of swimming larvae and settled recruits (with tissue) recorded. Due to the rapid breakdown of dead larvae (Yakovleva et al. 2009), larvae that could not be accounted for were assumed dead. Mortality was expressed as a percentage of the number of larvae added at the start of the experiment.</p>
<p>March 2012:<br />
The second experiment was designed to be virtually identical to the first (n = 8 tanks, 2 tanks treatment-1), although the volume of the incubation tanks was increased to 120 L, and the sample size (number of replicate tubs containing larvae in each tank) was doubled with the objective of increasing statistical power and testing for tank effects for the dependent biological variables. Experimental difficulties affecting a single tank made it problematic to include the tank in the statistical analyses, and therefore, replicates were pooled between tanks in each treatment combination. Larvae were sampled from each replicate tub, and their response to the treatment conditions was assessed using dark respiration, photophysiology (Fv/Fm), and mortality, and each dependent variable was measured as described above.</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO processing notes:</strong></p>
<p>- added conventional header with dataset name, PI name, version date, reference information<br />
- added lab, lat, lon columns<br />
- renamed parameters to BCO-DMO standard<br />
- combined data from Figures 1 and 2 (respiration Fv/Fm, and mortality)<br />
- reformated dates from m/d/yyyy to yyyy-mm-dd<br />
- replaced 'A'with 'ambient', 'H' with 'high'<br />
- converted mortality to percent</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
in-situ incubator
in-situ incubator
PI Supplied Instrument Name: in-situ incubator PI Supplied Instrument Description:A temperature-regulated bath (±0.1C, Hipoint, models LC-06) Instrument Name: In-situ incubator Instrument Short Name:in-situ incubator Instrument Description: A device on a ship or in the laboratory that holds water samples under controlled conditions of temperature and possibly illumination. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/82/
spectrophotometer
spectrophotometer
PI Supplied Instrument Name: spectrophotometer PI Supplied Instrument Description:USB2000, Ocean Optics Instrument Name: Spectrophotometer Instrument Short Name:Spectrophotometer Instrument Description: An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/
optode
optode
PI Supplied Instrument Name: optode PI Supplied Instrument Description:A ruthenium-based optrode (FOXY-R, 1.58 diameter, Ocean Optics) connected to a spectrophotometer (USB2000, Ocean Optics) and interfaced with a computer running Ocean Optics software (OOISensor, version 1.00.08). Instrument Name: Optode Instrument Short Name: Instrument Description: An optode or optrode is an optical sensor device that optically measures a specific substance usually with the aid of a chemical transducer.
Deployment: lab_Edmunds_NMMBA
lab_Edmunds_NMMBA
Natl Museum Mar. Bio. and Aquar. Taiwan
Natl Museum Mar. Bio. and Aquar. Taiwan
laboratory
lab_Edmunds_NMMBA
Peter J. Edmunds
California State University Northridge
Natl Museum Mar. Bio. and Aquar. Taiwan
Natl Museum Mar. Bio. and Aquar. Taiwan
laboratory