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            <gco:CharacterString>Cite this dataset as: Cowen, R. K., Kourafalou, V., Paris-Limouzy, C. B., Sponaugle, S. (2021) Multispecies larval otolith increment data from samples collected on R/V F.G. Walton Smith cruises WS0714, WS0720, WS0809 in the Straits of Florida from 2007-2008 (FK Population Connectivity project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2) Version Date 2016-10-04 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.529658.2 [access date]</gco:CharacterString>
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        <gco:CharacterString>Multispecies larval otolith increment data from the Straits of Florida, 2007-08. Dataset Description: &amp;lt;p&amp;gt;Otolith increment data from five species of coral reef fish collected in the Straits of Florida as 1) larvae in the plankton inside and outside of mesoscales eddies, 2) late-stage larvae over reefs, and 3) juveniles in reef and rubble habitats.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;em&amp;gt;Related publications and references:&amp;lt;/em&amp;gt;&amp;lt;br /&amp;gt;
Guigand CM, Cowen RK, Llopiz JK, Richardson DE (2005) A coupled asymmetrical multiple opening closing net with environmental sampling system. Mar. Tech. Soc. J 39, 22-24. doi:&amp;lt;a href=&amp;quot;http://dx.doi.org/10.4031/002533205787444042&amp;quot; target=&amp;quot;_blank&amp;quot;&amp;gt;10.4031/002533205787444042&amp;lt;/a&amp;gt;&amp;lt;br /&amp;gt;
&amp;lt;br /&amp;gt;
Shulzitski K, Sponaugle S, Hauff M, Walter K, D’Alessandro EK, Cowen RK (2015) Close encounters with eddies: oceanographic features increase growth of larval reef fishes during their journey to the reef. Biol Lett 20140746. doi:&amp;lt;a href=&amp;quot;http://dx.doi.org/10.1098/rsbl.2014.0746&amp;quot; target=&amp;quot;_blank&amp;quot;&amp;gt;10.1098/rsbl.2014.0746&amp;lt;/a&amp;gt;&amp;lt;br /&amp;gt;
&amp;lt;br /&amp;gt;
Shulzitski K, Sponaugle S, Hauff M, Walter K, Cowen RK (2016) Encounter with mesoscale eddies enhances survival to settlement in larval coral reef fishes. PNAS. doi:&amp;lt;a href=&amp;quot;http://dx.doi.org/10.1073/pnas.1601606113&amp;quot; target=&amp;quot;_blank&amp;quot;&amp;gt;10.1073/pnas.1601606113&amp;lt;/a&amp;gt;&amp;lt;/p&amp;gt; Methods and Sampling: &amp;lt;p&amp;gt;Ichthyoplankton samples were collected during three cruises aboard the R/V Walton Smith: WS-07-14 (May 29 - June 13, 2007), WS-07-20 (July 30 - August 13, 2007), and WS-08-09 (June 17 - July 1, 2008). During each cruise, samples were collected at seven stations along each of six cross-shelf transects spanning the western Straits of Florida. Ichthyoplankton was collected using a modified Multiple Opening Closing Net and Environmental Sensing System (MOCNESS, Guigand et al. 2005). The MOCNESS sampled discrete 20-m depth bins down to 80 m using paired nets (4 m2 and 1 m2) fitted with 1-mm and 150-μm mesh, respectively. All tows were conducted during daylight hours; samples were preserved immediately in 95% ethanol, and transferred to 70% ethanol upon returning to the laboratory. Specimens used in this study were all collected with the large-mesh nets (i.e., 1-mm). During and after the cruises (i.e., June – September 2007; July – September 2008), late-stage larvae and juveniles were sampled from American Shoal (AS) and Looe Key (LK) reefs in the lower Florida Keys. Four replicate light-traps were deployed at each reef 1 m below the surface and 50 m apart. Traps fished from sunset to sunrise during 15-d periods encompassing both the new and third-quarter lunar phases, when most coral reef fishes settle. One week into light-trap sampling, juvenile collections were initiated by SCUBA divers using quinaldine and hand nets.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;The investigators followed standard procedures for analyzing otolith microstructure of a subset of fish from five species (Xyrichtys novacula, Thalassoma bifasciatum, Cryptotomus roseus, Sphyraena barracuda, and Stegastes partitus) to obtain individual growth rates and ages. Standard length (SL) or notochord length (NL) was measured to the nearest 0.01 mm for each fish using a Leica MZ12 dissecting microscope, a Cool Snap-Pro monochrome digital camera, and Image-Pro Plus 4.5 image analysis software (Media Cybernetics). Sagittal (X. novacula, T. bifasciatum, and C. roseus) or lapillar (S. partitus) otoliths were dissected from each sample and stored in immersion oil ~7-14 days while lapillar otoliths of S. barracuda were dissected and sectioned to facilitate reading. All otoliths from a given species were analyzed by a single reader. Otoliths were read along the longest axis at 400X magnification (with the exception of S. barracuda lapilli which were read at 1000X magnification) through a Leica DMLB microscope and with the aid of the digital camera and Image-Pro Plus software. All otoliths were read at least twice, and if the reads differed by ≤ 5%, one read was randomly chosen for analysis. If reads differed by &amp;amp;gt; 5%, a third read was conducted. This third read was then compared to the first two reads. If either comparison differed by ≤ 5%, one read from that comparison was randomly chosen for analysis; otoliths where all reads differed by &amp;amp;gt; 5% were removed from any further analysis.&amp;lt;/p&amp;gt;</gco:CharacterString>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/521581.rdf" xlink:title="OCE-0550732" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0550732 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0550732</gmx:Anchor>
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This project deals with the important and timely theme of marine population connectivity. The degree to which populations of benthic marine organisms are connected via the dispersal of larval propagules is a central unanswered ecological and oceanographic question. The complex oceanography of marine systems, and high mortality and diffuse concentrations of larvae make direct measurement of larval sources generally unfeasible, particularly for marine populations distributed along open coastlines. In addition, ecological population connectivity is not only a function of the physical transport of larvae, but also the interaction of factors influencing larval growth, survival, and condition at settlement. For example, oligotrophic open-ocean environments may lead to slower larval growth, longer pelagic larval durations, and lower survivorship of larvae compared to larvae from nutrient-rich nearshore waters. Data indicate that the relative condition of larvae influences their survival on the reef and the degree to which they contribute to the population. Ultimately, as ocean currents, spawning patterns, larval survivorship, settlement, and their interactions are highly variable, the only method for examining ecological population connectivity over multiple time and space scales in oceanographically complex environments will be data-validated three dimensional biophysical models capable of assessing dispersal outcomes over a wide range of temporal and spatial variation. &lt;/p&gt;
&lt;p&gt;The overall goal of this study is to quantify the relative contributions of upstream (far-field) versus local (near-field) sources of reef fish larvae to the Florida Keys. The proposed study will integrate a comprehensive, three dimensional hydrodynamic model with a Lagrangian particle tracking model to connect the pathways between observed ichthyoplankton distributions and larval settlement. &lt;/p&gt;</gco:CharacterString>
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            http://lod.bco-dmo.org/id/dataset-parameter/660492.rdf
	Name: cruise_id
	Units: unitless
	Description: &lt;p&gt;Official cruise identifier&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/660493.rdf
	Name: cruise
	Units: unitless
	Description: &lt;p&gt;Designates the cruise during which the larva was collected. The dates of the cruises are as follows: June 2007 (May 29 - June 13), August 2007 (July 30 - August 13), and June 2008 (June 17 - July 1). Hatch window was calculted for all larvae, late-stage larvae, and juveniles. Thus, the cruise designation for each late-stage larva and juvenile was based on the cruise designation of larvae included in their 30 day hatch window.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/660494.rdf
	Name: species
	Units: unitless
	Description: &lt;p&gt;Species name assigned to each individual based on morphological or genetic identification.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/660495.rdf
	Name: life_stage
	Units: unitless
	Description: &lt;p&gt;Life stage assigned to each individual. Each &quot;larva&quot; was collected from the plankton using a modified MOCNESS net system; each &quot;Late-stage larva&quot; was collected using light traps that intercept larva as they settle to the reef; each juvenile was collected from reef or rubble habitat using SCUBA, quinaldine, and hand nets.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/660496.rdf
	Name: eddy_group
	Units: unitless
	Description: &lt;p&gt;Refers to the group assigned to each larva determined by whether or not the station in which it was collected was located inside a mesoscale eddy (&quot;ED&quot;) or well outside of a mesoscale eddy (&quot;NE&quot;). Late-stage larvae and juveniles were all assigned to the survivor group (&quot;SUR&quot;).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/660497.rdf
	Name: individual
	Units: unitless
	Description: &lt;p&gt;Refers to the unique number assigned to each individual larva, late-stage larva, or juvenile.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/660498.rdf
	Name: sample
	Units: unitless
	Description: &lt;p&gt;Refers to the sample number assigned to the MOCNESS net in which the larva was collected, the light trap in which the late-stage larva was collected, or the reef and rubble juvenile collection.&lt;/p&gt;
&lt;p&gt;Sample formats:&lt;br /&gt;
e.g.: (WS-07-20)M-44-046(M4:20-40)&lt;br /&gt;
      WS-07-20 = Cruise ID&lt;br /&gt;
      M = Sampling Gear ID (M=MOCNESS)&lt;br /&gt;
      44 = Master Station&lt;br /&gt;
      46 = Sequential Station&lt;br /&gt;
      M4 = Net Type (M4=4 square meter MOCNESS frame)&lt;br /&gt;
      20 = Mininum target depth of tow&lt;br /&gt;
      40 = Maximum target depth of tow&lt;/p&gt;
&lt;p&gt;e.g.: AS070712-1&lt;br /&gt;
      AS = Site designation (AS=American Shoal reef; LK=Looe Key reef)&lt;br /&gt;
      070712 = Date (year, month, day)&lt;br /&gt;
      1 = Collection number&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/660499.rdf
	Name: std_len
	Units: millimeters (mm)
	Description: &lt;p&gt;Refers to standard length measured in mm.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/660500.rdf
	Name: larval_age
	Units: days
	Description: &lt;p&gt;Refers to the larval age in days. This is calculated as the total number of increments minus one, since the first increment number designates the core of the otolith.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/660501.rdf
	Name: avg_recent_growth
	Units: micrometers (um)
	Description: &lt;p&gt;Refers to the average of the last three full daily otolith incrememts measured in um.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/660502.rdf
	Name: date_gmt
	Units: unitless
	Description: &lt;p&gt;The GMT date of collection in mm/dd/yyyy format.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/660503.rdf
	Name: lat
	Units: decimal degrees
	Description: &lt;p&gt;The latitude in decimal degrees of the sampling station.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/660504.rdf
	Name: lon
	Units: decimal degrees
	Description: &lt;p&gt;The longitude in decimal degrees of the sampling station.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/660505.rdf
	Name: hatch_date
	Units: unitless
	Description: &lt;p&gt;Date of hatch calcluated by subtracting the total age from the collection date; in mm/dd/yyyy format.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/660506.rdf
	Name: juv_age
	Units: days
	Description: &lt;p&gt;Refers to the number of juvenile increments enumerated on the otolith.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/660507.rdf
	Name: total_age
	Units: days
	Description: &lt;p&gt;Refers to the total age. For larvae and late-stage larvae this is the same as the larval age. For juveniles this is calculated by adding the larval and juvenile ages together.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/660508.rdf
	Name: last_otolith_radius
	Units: micrometers (um)
	Description: &lt;p&gt;Refers to outermost otolith radius (distance from the core to the edge of the otolith) measured in um.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/660509.rdf
	Name: increment_num
	Units: unitless
	Description: &lt;p&gt;Refers to the number of larval increments marked on the otolith (for larvae and late-stage larvae all increments are larval increments). The first increment is marked at the core of the otolith and the final increment is marked at the outermost edge of the otolith (for larvae and late-stage larvae) or at the inside of the metamorphic band (for juveniles of Thalassoma bifasciatum).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/660510.rdf
	Name: larval_otolith_radius
	Units: micrometers (um)
	Description: &lt;p&gt;Refers to larval otolith radius (distance from the core to outside edge of the larval increment) measured in um.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/660511.rdf
	Name: increment_width
	Units: micrometers (um)
	Description: &lt;p&gt;Refers to width of each larval increment (um). The outermost larval increment for larvae and late-stage larvae may not be fully formed so was removed from the dataset.&lt;/p&gt; 
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&amp;lt;p&amp;gt;The investigators followed standard procedures for analyzing otolith microstructure of a subset of fish from five species (Xyrichtys novacula, Thalassoma bifasciatum, Cryptotomus roseus, Sphyraena barracuda, and Stegastes partitus) to obtain individual growth rates and ages. Standard length (SL) or notochord length (NL) was measured to the nearest 0.01 mm for each fish using a Leica MZ12 dissecting microscope, a Cool Snap-Pro monochrome digital camera, and Image-Pro Plus 4.5 image analysis software (Media Cybernetics). Sagittal (X. novacula, T. bifasciatum, and C. roseus) or lapillar (S. partitus) otoliths were dissected from each sample and stored in immersion oil ~7-14 days while lapillar otoliths of S. barracuda were dissected and sectioned to facilitate reading. All otoliths from a given species were analyzed by a single reader. Otoliths were read along the longest axis at 400X magnification (with the exception of S. barracuda lapilli which were read at 1000X magnification) through a Leica DMLB microscope and with the aid of the digital camera and Image-Pro Plus software. All otoliths were read at least twice, and if the reads differed by ≤ 5%, one read was randomly chosen for analysis. If reads differed by &amp;amp;gt; 5%, a third read was conducted. This third read was then compared to the first two reads. If either comparison differed by ≤ 5%, one read from that comparison was randomly chosen for analysis; otoliths where all reads differed by &amp;amp;gt; 5% were removed from any further analysis.&amp;lt;/p&amp;gt;</gco:CharacterString>
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&amp;lt;p&amp;gt;BCO-DMO Processing Notes:&amp;lt;br /&amp;gt;
- Modified parameter names to conform with BCO-DMO naming conventions;&amp;lt;br /&amp;gt;
- Added the official cruise identifier in the cruise_id column;&amp;lt;br /&amp;gt;
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Guigand, C.M., Cowen, R.K., Llopiz, J.K., and Richardson, D.E. 2005. A coupled asymmetrical multiple opening closing net with environmental sampling systems. Mar. Technol. Soc. J. 39(2): 22–24. doi:10.4031/002533205787444042. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/NETT0097/</gco:CharacterString>
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