Respiration of brooded coral larvae at high and ambient temperature and pCO2, Taiwan, March 2011 (Cumbo et al, JEMBE, 2013) (MCR LTER & Climate Coral Larvae projects)

Website: https://www.bco-dmo.org/dataset/535266
Data Type: experimental
Version: 1
Version Date: 2014-10-07

Project
» Moorea Coral Reef Long-Term Ecological Research site (MCR LTER)
» The ecophysiological basis of the response of coral larvae and early life history stages to global climate change (Climate_Coral_Larvae)

Program
» Long Term Ecological Research network (LTER)
ContributorsAffiliationRole
Edmunds, Peter J.California State University Northridge (CSUN)Principal Investigator
Cumbo, Vivian RCalifornia State University Northridge (CSUN)Co-Principal Investigator
Fan, Tung-YungNational Museum of Marine Biology and Aquarium (NMMBA)Co-Principal Investigator
Copley, NancyWoods Hole Oceanographic Institution (WHOI BCO-DMO)BCO-DMO Data Manager

Abstract
The physiological development of brooded larvae from the pocilloporid corals Pocillopora damicornis in southern Taiwan under elevated temperature and pCO2 was examined. These data include respiration rates of brooded coral larvae at high and ambient temperature and pCO2 conducted in March 2011. These data were published in Cumbo et al, JEMBE, 2013.


Coverage

Spatial Extent: Lat:21.93817 Lon:120.74602
Temporal Extent: 2011-03-02 - 2011-03-18

Methods & Sampling

Larval respiration was measured in a confined respirometer use a Ruthenium-based optrode (FOXY-R, 1.58 diameter, Ocean Optics) connected to a spectrophotometer (USB2000, Ocean Optics) and interfaced with a computer running the manufacturers software (OOISensor, version 1.00.08). The optrode was calibrated using a zero solution (0.01 M Na2B4O7·10H2O supersaturated with Na2SO3) and 100% air saturation usingwater-saturated air at the treatment temperature. Tomeasure respiration, 6 larvae were removed from the tubs in the treatment tanks and placed into 2-mL glass Wheaton vials filled with filtered seawater from the same treatment tank and sealed with Parafilm. A study conducted concurrently with the present analysis demonstrated that respiration of P. damicornis larvae in identical vials could be measured accurately with 5 larvae vial-1 (Edmunds et al., 2011).

Initial O2 concentration in the seawater was determined before the vials were sealed, and vials without larvae used as controls. Larvae in the sealed vials were incubated at their temperature treatments for 1.5-2 h in the dark using water baths set to the temperature of the respective treatments (±0.1 °C, Hipoint, models LC-06 and LC-10); vials were carefully moved throughout the incubations to ensure the seawater remained mixed. Incubation timeswere selected to ensure O2 concentrations remained >75% saturation. On completion of the incubations, vials were removed from the water baths, gently inverted to mix the seawater, and analyzed for O2 saturation. O2 saturation was converted to concentration using tabulated values of O2 solubility (N. Ramsing and J. Gundersen at http://www.unisense.com [based on Garcia and Gordon, 1992]) and the temperature and salinity of the seawater, and the change in O2 concentration used to calculate O2 flux after adjusting for controls. The respiration rates are expressed as nmol O2 mg protein-1 min-1.

The 'ambient' and 'high' pCO2 levels: 49.4 Pa versus 86.2 Pa
The 'ambient' and 'high' temperatures: 24.00 °C [ambient] versus 30.49 °C

Data also available from PANGAEA: doi:10.1594/PANGAEA.823582


Data Processing Description

BCO-DMO processing notes:

- added conventional header with dataset name, PI name, version date, reference information
- renamed parameters to BCO-DMO standard
- added lab, lat, lon columns
- reformated date from d-Mon-yy to yyyy-mm-dd
- replaced blank cells with N_A
- replaced blanks and within cells with underscores
- changed Ambient to ambient; High to high; P. dam to Pocillopora_damicornis


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Data Files

File
brood3_respraw.csv
(Comma Separated Values (.csv), 12.35 KB)
MD5:868a47f29d2182bdbd87428aad0b8a7f
Primary data file for dataset ID 535266

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Supplemental Files

File
Biological data for "brooded coral larvae expt. 3" datasets
filename: Cumbo_etal_2012_JEMBE_data1_BCODMO.xls
(Octet Stream, 154.50 KB)
MD5:e6c5e6012df9bfc581b9f769f7e52a98
Original biological data for Cumbo et al 2013 including respiration raw data, respiration by mg protein, symbiont densities, protein content, % mortality
Tank physical data
filename: Cumbo_etal_2012_JEMBE_Tank_Parameters_BCODMO.xlsx
(Octet Stream, 57.05 KB)
MD5:0170402805d7c1fe4784a51d2b26fb66
Tank physical data for "brooded coral larvae 3" experiment including seawater chemistry, light and temperature data.

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Related Publications

Cumbo, V. R., Fan, T. Y., & Edmunds, P. J. (2013). Effects of exposure duration on the response of Pocillopora damicornis larvae to elevated temperature and high pCO2. Journal of Experimental Marine Biology and Ecology, 439, 100–107. doi:10.1016/j.jembe.2012.10.019
Results
Edmunds, P. J., Cumbo, V., & Fan, T.-Y. (2011). Effects of temperature on the respiration of brooded larvae from tropical reef corals. Journal of Experimental Biology, 214(16), 2783–2790. doi:10.1242/jeb.055343
Results
Garcia, H. E., & Gordon, L. I. (1992). Oxygen solubility in seawater: Better fitting equations. Limnology and Oceanography, 37(6), 1307–1312. doi:10.4319/lo.1992.37.6.1307
General

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Related Datasets

IsRelatedTo
Edmunds, P. J., Cumbo, V. R., Fan, T. (2014) Light data in tanks from experiment on brooded coral larval, Taiwan, March 2011 (Cumbo et al, JEMBE, 2013) (MCR LTER & Climate Coral Larvae projects). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2014-10-07 http://lod.bco-dmo.org/id/dataset/535219 [view at BCO-DMO]
Edmunds, P. J., Cumbo, V. R., Fan, T. (2014) Protein content of brooded coral larvae at high and ambient temperature and pCO2, March 2011 (Cumbo et al, JEMBE, 2013) (MCR LTER & Climate Coral Larvae projects). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2014-10-07 http://lod.bco-dmo.org/id/dataset/535425 [view at BCO-DMO]
Edmunds, P. J., Cumbo, V. R., Fan, T. (2014) Respiration and protein content of brooded coral larvae at high and ambient temperature and pCO2, Taiwan, March 2011 (Cumbo et al, JEMBE, 2013) (MCR LTER & Climate Coral Larvae projects). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2014-10-07 http://lod.bco-dmo.org/id/dataset/535328 [view at BCO-DMO]
Edmunds, P. J., Cumbo, V. R., Fan, T. (2014) Seawater carbonate chemistry from experiment on brooded coral larval, March 2011, Taiwan (Cumbo et al, JEMBE, 2013) (MCR LTER & Climate Coral Larvae projects). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2014-10-07 http://lod.bco-dmo.org/id/dataset/535163 [view at BCO-DMO]
Edmunds, P. J., Cumbo, V. R., Fan, T. (2014) Symbiont Symbiodinium density in brooded coral larvae at high and ambient temperature and pCO2, Taiwan, March 2011 (Cumbo et al, JEMBE, 2013) (MCR LTER & Climate Coral Larvae projects). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2014-10-07 http://lod.bco-dmo.org/id/dataset/535358 [view at BCO-DMO]
Edmunds, P. J., Cumbo, V. R., Fan, T. (2014) Temperature data from tanks from experiment on brooded coral larval, Taiwan, March 2011 (Cumbo et al, JEMBE, 2013) (MCR LTER & Climate Coral Larvae projects). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2014-10-07 http://lod.bco-dmo.org/id/dataset/535244 [view at BCO-DMO]
Edmunds, P. J., Cumbo, V. R., Fan, T. (2021) Settling and mortality measurements of brooded coral larvae at high and ambient temperature and pCO2, Taiwan, March 2011 (MCR LTER project, Climate_Coral_Larvae project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2014-10-07 doi:10.26008/1912/bco-dmo.535462.1 [view at BCO-DMO]

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Parameters

ParameterDescriptionUnits
lab

laboratory

unitless
lat

latitude; north is positive

decimal degrees
lon

longitude; east is positive

decimal degrees
date

local date

yyyy-mm-dd
treatment

with or without larvae

unitless
sample

sample identification

unitless
tank

tank id number

tank
temp

target temperature

degrees Celsius
treatment_pCO2

pCO2 treatment: ambient (47.5 - 49.3 Pa) or high (85.2 - 87.2 Pa)

unitless
days

days since start of experiment

unitless
num_larvae

number of larvae in respiration chamber

integer
species

coral species name

unitless
vol_chamber

the volume in which the cells were resuspended prior to counting

ml
time_inc

length of incubation

minutes
O2_sat_pcent_start

oxygen saturation relative to air at start of incubation

percent
O2_sat_pcent_final

oxygen saturation relative to air at end of incubation

percent
resp_chamber

respiration rate (6 larvae/chamber)

umol O2/chamber/hr
resp_larv

respiration rate per larva

nmol O2/larva/min
resp_control

average respiration rate of the 4 control vials

micromol O2/chamber/hour
comment

comments

unitless


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Instruments

Dataset-specific Instrument Name
Aquarium chiller
Generic Instrument Name
Aquarium chiller
Dataset-specific Description
Aquatech Ac11 or Shyeh Duwai Enterprise
Generic Instrument Description
Immersible or in-line liquid cooling device, usually with temperature control.

Dataset-specific Instrument Name
Gas Analyzer
Generic Instrument Name
Gas Analyzer
Dataset-specific Description
Infra Red gas analyzer (S151, Qubit Systems)
Generic Instrument Description
Gas Analyzers - Instruments for determining the qualitative and quantitative composition of gas mixtures.

Dataset-specific Instrument Name
Immersion heater
Generic Instrument Name
Immersion heater
Dataset-specific Description
300 Wheaters, Taikong Corporation
Generic Instrument Description
Submersible heating element for water tanks and aquaria.

Dataset-specific Instrument Name
LI-COR LI-192 light sensor
Generic Instrument Name
LI-COR LI-192 PAR Sensor
Dataset-specific Description
cosine-corrected quantum light meter (Li-Cor LI-192 attached to an LI-1400)
Generic Instrument Description
The LI-192 Underwater Quantum Sensor (UWQ) measures underwater or atmospheric Photon Flux Density (PPFD) (Photosynthetically Available Radiation from 360 degrees) using a Silicon Photodiode and glass filters encased in a waterproof housing. The LI-192 is cosine corrected and features corrosion resistant, rugged construction for use in freshwater or saltwater and pressures up to 800 psi (5500 kPa, 560 meters depth). Typical output is in um s-1 m-2. The LI-192 uses computer-tailored filter glass to achieve the desired quantum response. Calibration is traceable to NIST. The LI-192 serial numbers begin with UWQ-XXXXX. LI-COR has been producing Underwater Quantum Sensors since 1973. These LI-192 sensors are typically listed as LI-192SA to designate the 2-pin connector on the base of the housing and require an Underwater Cable (LI-COR part number 2222UWB) to connect to the pins on the Sensor and connect to a data recording device. The LI-192 differs from the LI-193 primarily in sensitivity and angular response. 193: Sensitivity: Typically 7 uA per 1000 umol s-1 m-2 in water. Azimuth: < ± 3% error over 360° at 90° from normal axis. Angular Response: < ± 4% error up to ± 90° from normal axis. 192: Sensitivity: Typically 4 uA per 1000 umol s-1 m-2 in water. Azimuth: < ± 1% error over 360° at 45° elevation. Cosine Correction: Optimized for underwater and atmospheric use. (www.licor.com)

Dataset-specific Instrument Name
optrode
Generic Instrument Name
Optode
Dataset-specific Description
A Ruthenium-based optrode (FOXY-R, 1.58 diameter, Ocean Optics) connected to a spectrophotometer (USB2000, Ocean Optics) and interfaced with a computer running the manufacturers software (OOISensor, version 1.00.08). The optrode was calibrated using a zero solution (0.01 M Na2B4O7·10H2O supersaturated with Na2SO3) and 100% air saturation using water-saturated air at the treatment temperature.
Generic Instrument Description
An optode or optrode is an optical sensor device that optically measures a specific substance usually with the aid of a chemical transducer.

Dataset-specific Instrument Name
spectrophotometer
Generic Instrument Name
Spectrophotometer
Dataset-specific Description
USB2000, Ocean Optics
Generic Instrument Description
An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples.

Dataset-specific Instrument Name
Water Temp Sensor
Generic Instrument Name
Water Temperature Sensor
Dataset-specific Description
certified digital thermometer (Model 15-077-8, Fisher Scientific,±0.05 °C)
Generic Instrument Description
General term for an instrument that measures the temperature of the water with which it is in contact (thermometer).


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Deployments

lab_Edmunds_NMMBA

Website
Platform
Natl Museum Mar. Bio. and Aquar. Taiwan
Start Date
2010-03-18
End Date
2010-03-24
Description
Experiments related to the research project:  'RUI- The ecophysiological basis of the response of coral larvae and early life history stages to global climate change' were conducted at the laboratories of the National Museum of Marine Biology and Aquarium in Southern Taiwan.


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Project Information

Moorea Coral Reef Long-Term Ecological Research site (MCR LTER)


Coverage: Island of Moorea, French Polynesia


From http://www.lternet.edu/sites/mcr/ and http://mcr.lternet.edu/:
The Moorea Coral Reef LTER site encompasses the coral reef complex that surrounds the island of Moorea, French Polynesia (17°30'S, 149°50'W). Moorea is a small, triangular volcanic island 20 km west of Tahiti in the Society Islands of French Polynesia. An offshore barrier reef forms a system of shallow (mean depth ~ 5-7 m), narrow (~0.8-1.5 km wide) lagoons around the 60 km perimeter of Moorea. All major coral reef types (e.g., fringing reef, lagoon patch reefs, back reef, barrier reef and fore reef) are present and accessible by small boat.

The MCR LTER was established in 2004 by the US National Science Foundation (NSF) and is a partnership between the University of California Santa Barbara and California State University, Northridge. MCR researchers include marine scientists from the UC Santa Barbara, CSU Northridge, UC Davis, UC Santa Cruz, UC San Diego, CSU San Marcos, Duke University and the University of Hawaii. Field operations are conducted from the UC Berkeley Richard B. Gump South Pacific Research Station on the island of Moorea, French Polynesia.

MCR LTER Data: The Moorea Coral Reef (MCR) LTER data are managed by and available directly from the MCR project data site URL shown above.  The datasets listed below were collected at or near the MCR LTER sampling locations, and funded by NSF OCE as ancillary projects related to the MCR LTER core research themes.

This project is supported by continuing grants with slight name variations:

  • LTER: Long-Term Dynamics of a Coral Reef Ecosystem
  • LTER: MCR II - Long-Term Dynamics of a Coral Reef Ecosystem
  • LTER: MCR IIB: Long-Term Dynamics of a Coral Reef Ecosystem
  • LTER: MCR III: Long-Term Dynamics of a Coral Reef Ecosystem
  • LTER: MCR IV: Long-Term Dynamics of a Coral Reef Ecosystem

The ecophysiological basis of the response of coral larvae and early life history stages to global climate change (Climate_Coral_Larvae)

Coverage: Moorea, French Polynesia; Southern Taiwan; California State University Northridge


Tropical coral reefs face a suite of environmental assaults ranging from anchor damage to the effects of global climate change (GCC). The consequences are evident throughout the tropics, where many coral reefs have lost a substantial fraction of their coral cover in a few decades. Notwithstanding the importance of reducing the impacts of environmental stresses, the only means by which these ecosystems can recover (or simply persist) is through the recruitment of scleractinians, which is a function of successful larval development, delivery, settlement, metamorphosis, and post-settlement events. Despite wide recognition of the importance of these processes, there are few pertinent empirical data, and virtually none that address the mechanisms mediating the success of early coral life stages in a physical environmental varying at multiple spatio-temporal scales.

The objective of this research is to complete one of the first comprehensive ecophysiological analyses of the early life stages of corals through a description of: (1) their functionality under 'normal' conditions, and (2) their response to the main drivers of GCC. These analyses will be completed for 2 species representative of a brooding life history strategy, and the experiments will be completed in two locations, one (Taiwan) that provides unrivalled experience in coral reproductive biology, and superb microcosm facilities, and the other (Moorea), with access to a relatively pristine environment, a well described ecological and oceanographic context (through the MCR-LTER), and the capacity to bring a strong biogeographic contrast to the project. The results of the study will be integrated through modeling to explore the effects of GCC on coral community structure over the next century.

The following publications and data resulted from this project:

2013    Wall CB, Fan TY, Edmunds PJ.  Ocean acidification has no effect on thermal bleaching in the coral Seriatopora caliendrum.  Coral Reefs 33: 119-130.
Symbiodinium_Seriatopora photosynthesis
Symbiodinium_Seriatopora PI curve
Symbiodinium_Seriatopora temp-salinity-light
Symbiodinium_Seriatopora water chemistry
- Download complete data for this publication (Excel file)

2013    Wall CB, Edmunds PJ. In situ effects of low pH and elevated HCO3- on juvenile Porites spp. in Moorea, French Polynesia.  Biological Bulletin 225:92-101.
Data at MCR and PANGEA: doi.pangaea.de/10.1594/PANGAEA.833913
- Download complete data for this publication (Excel file)

2013    Vivian R Cumbo, Peter J Edmunds, Christopher B Wall, Tung-Yung Fan. Brooded coral larvae differ in their response to high temperature and elevated pCO2 depending on the day of release.  Marine Biology DOI 10.1007/s00227-013-2280-y.
Data also at PANGEA: doi.pangaea.de/10.1594/PANGAEA.831612
brooded coral larvae 2 - carbonate chemistry
brooded coral larvae 2 - larval release March 2003-2008
brooded coral larvae 2 - respiration_photosyth_mortality
- Download complete data for this publication (Excel file)

2013    Edmunds PJ, Cumbo VR, Fan TY. Metabolic costs of larval settlement and metamorphosis in the coral Seriatopora caliendrum under ambient and elevated pCO2.  Journal Experimental Marine Biology and Ecology 443: 33-38 Data also at PANGEA: doi:10.1594/PANGAEA.821644
Coral post-settlement physiology
- Download complete data for this publication (Excel file)

2013    Aaron M Dufault, Aaron Ninokawa, Lorenzo Bramanti, Vivian R Cumbo, Tung-Yung Fan, Peter J Edmunds.  The role of light in mediating the effects of ocean acidification on coral calcification.  Journal of Experimental Biology 216: 1570-1577.
coral-light expt.- PAR
coral-light expt.- carbonate chemistry
coral-light expt.- temp_salinity
coral-light expt.- growth
coral-light expt.- protein
coral-light expt.- survival
- Download complete data for this publication (Excel file)

2012    Cumbo, VR, Fan TY, Edmunds PJ. Effects of exposure duration on the response of Pocillopora damicornis larvae to elevated temperature and high pCO2.  J Exp Mar Biol Ecol 439: 100-107.
Data is also at PANGEA: doi:10.1594/PANGAEA.823582
brooded coral larvae 3 - carbonate chemistry
brooded coral larvae 3 - light
brooded coral larvae 3 - mortality
brooded coral larvae 3 - protein
brooded coral larvae 3 - respiration and protein
brooded coral larvae 3 - respiration raw data
brooded coral larvae 3 - symbiont density
brooded coral larvae 3 - tank temperature
- Download part 1 of data for this publication (Excel file)
- Download tank parameters data for this publication (Excel file)

2012    Cumbo, VR, Fan TY, Edmunds PJ.  Physiological development of brooded larvae from two pocilloporid corals in Taiwan.  Marine Biology 159: 2853-2866.
brooded coral - carbonate chemistry
brooded coral - release
brooded coral - respiration
brooded coral - settlement competency
brooded coral - size_July
brooded coral - size_protein_symbionts_photosynth
- Download complete data for this publication (Excel file)

2012    Dufault, Aaron M; Vivian R Cumbo; Tung-Yung Fan; Peter J Edmunds.  Effects of diurnally oscillating pCO2 on the calcification and survival of coral recruits.  Royal Society of London (B) 279: 2951-2958.  doi:10.1098/rspb.2011.2545
Data is also at PANGEA: doi:10.1594/PANGAEA.830185
recruit_growth_area
recruit_growth_weight
recruit_seawater_chemistry
recruit_survival
- Download complete data for this publication (Excel file)

2011    Edmunds PJ, Cumbo V, Fan TY.  Effects of temperature on the respiration of brooded larvae from tropical reef corals.  Journal of Experimental Biology 214: 2783-2790. 
CoralLarvae_comparison_respir
CoralLarvae_release
CoralLarvae_respir
CoralLarvae_size
- Download complete data for this publication (Excel file)



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Program Information

Long Term Ecological Research network (LTER)


Coverage: United States


adapted from http://www.lternet.edu/

The National Science Foundation established the LTER program in 1980 to support research on long-term ecological phenomena in the United States. The Long Term Ecological Research (LTER) Network is a collaborative effort involving more than 1800 scientists and students investigating ecological processes over long temporal and broad spatial scales. The LTER Network promotes synthesis and comparative research across sites and ecosystems and among other related national and international research programs. The LTER research sites represent diverse ecosystems with emphasis on different research themes, and cross-site communication, network publications, and research-planning activities are coordinated through the LTER Network Office.

LTER site location map

2017 LTER research site map obtained from https://lternet.edu/site/lter-network/



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Funding

Funding SourceAward
NSF Division of Ocean Sciences (NSF OCE)

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