Symbiont Symbiodinium density in brooded coral larvae at high and ambient temperature and pCO2, Taiwan, March 2011 (Cumbo et al, JEMBE, 2012) (MCR LTER project, Climate_Coral_Larvae project)

Website: https://www.bco-dmo.org/dataset/535358
Version: 2014-10-07

Project
» Moorea Coral Reef Long-Term Ecological Research site (MCR LTER)
» The ecophysiological basis of the response of coral larvae and early life history stages to global climate change (Climate_Coral_Larvae)

Program
» Long Term Ecological Research network (LTER)
ContributorsAffiliationRole
Edmunds, Peter J.California State University Northridge (CSU-Northridge)Principal Investigator
Cumbo, Vivian RCalifornia State University Northridge (CSU-Northridge)Co-Principal Investigator
Fan, Tung-YungNational Museum of Marine Biology and Aquarium (NMMBA)Co-Principal Investigator
Copley, NancyWoods Hole Oceanographic Institution (WHOI BCO-DMO)BCO-DMO Data Manager

Table of Contents


Dataset Description

The physiological development of brooded larvae from the pocilloporid corals Pocillopora damicornis in southern Taiwan under elevated temperature and pCO2 was examined.

These data include symbiont Symbiodinium density in brooded coral larvae at high and ambient temperature and pCO2 conducted in March 2011.

Related datasets:

brooded coral larvae 3 - carbonate chemistry
brooded coral larvae 3 - light
brooded coral larvae 3 - mortality
brooded coral larvae 3 - protein
brooded coral larvae 3 - respiration and protein
brooded coral larvae 3 - respiration raw data
brooded coral larvae 3 - tank temperature

These data are published in Cumbo, VR, Fan TY, Edmunds PJ. Effects of exposure duration on the response of Pocillopora damicornis larvae to elevated temperature and high pCO2. J Exp Mar Biol Ecol 439: 100-107.

Download tank physical data (Excel file)
Download biological data for this publication (Excel file)
Data also available from PANGAEA: doi:10.1594/PANGAEA.823582


Acquisition Description

Symbiodinium densities were determined by preserving 2 groups of 6 larvae fromeach tank (one from each tub) in 100 uL of 10% formalin in FSW, and macerating them with a Teflon pestle. The Symbiodinium in the slurry were counted using a hemocytometer (4 replicate counts) and the algal density expressed as cells mg protein-1. Preliminary analyses demonstrated that the mean and SE of the Symbiodinium counts stabilized at 4 replicate counts, and therefore additional replicate counts were not considered necessary. Quality control was conducted by screening the data for cases where the SE of the counts was >20% of the mean, and in rare cases where this occurred, counts were repeated.


Processing Description

BCO-DMO processing notes:

- added conventional header with dataset name, PI name, version date, reference information
- renamed parameters to BCO-DMO standard
- added lab, lat, lon columns
- reformated date from d-Mon-yy to yyyy-mm-dd
- changed Ambient to ambient; High to high; P. dam to Pocillopora_damicornis
- reduced number of significant digits
- replaced blanks with underscores


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Parameters

ParameterDescriptionUnits
lablaboratory unitless
latlatitude; north is positive decimal degrees
lonlongitude; east is positive decimal degrees
datelocal date yyyy-mm-dd
samplesample identification unitless
tanktank id number tank
temptarget temperature degrees Celsius
treatment_pCO2pCO2 treatment: ambient (419-470 uatm) or high (604-742 uatm) unitless
daysdays since start of experiment unitless
num_larvaenumber of larvae examined integer
vol_chamberthe volume in which the cells were resuspended prior to counting ml
vol_gridvolume of grid ml
rep_1number of symbionts in replicate 1 of hemocytometer slide integer
rep_2number of symbionts in replicate 2 of hemocytometer slide integer
rep_3number of symbionts in replicate 3 of hemocytometer slide integer
rep_4number of symbionts in replicate 4 of hemocytometer slide integer
count_meanaverage number of symbionts in samples unitless
count_stdevstandard deviation: number of symbionts in samples unitless
count_sestandard error: number of symbionts in samples unitless
CV? percent?
cells_totaltotal number of symbionts from the 4 replicates unitless
cells_larvaaverage number of symbionts per larva cells/larva
protein_totaltotal protein from all 6 larvae in incubation chamber mg?
cells_mg_proteinnumber of symbionts per milligram protein cells/mg protein

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Instruments

Dataset-specific Instrument NameLI-COR LI-192 light sensor
Generic Instrument NameLI-COR LI-192 PAR Sensor
Dataset-specific Descriptioncosine-corrected quantum light meter (Li-Cor LI-192 attached to an LI-1400)
Generic Instrument DescriptionThe LI-192 Underwater Quantum Sensor (UWQ) measures underwater or atmospheric Photon Flux Density (PPFD) (Photosynthetically Available Radiation from 360 degrees) using a Silicon Photodiode and glass filters encased in a waterproof housing. The LI-192 is cosine corrected and features corrosion resistant, rugged construction for use in freshwater or saltwater and pressures up to 800 psi (5500 kPa, 560 meters depth). Typical output is in um s-1 m-2. The LI-192 uses computer-tailored filter glass to achieve the desired quantum response. Calibration is traceable to NIST. The LI-192 serial numbers begin with UWQ-XXXXX. LI-COR has been producing Underwater Quantum Sensors since 1973. These LI-192 sensors are typically listed as LI-192SA to designate the 2-pin connector on the base of the housing and require an Underwater Cable (LI-COR part number 2222UWB) to connect to the pins on the Sensor and connect to a data recording device. The LI-192 differs from the LI-193 primarily in sensitivity and angular response. 193: Sensitivity: Typically 7 uA per 1000 umol s-1 m-2 in water. Azimuth: < ± 3% error over 360° at 90° from normal axis. Angular Response: < ± 4% error up to ± 90° from normal axis 192: Sensitivity: Typically 4 uA per 1000 umol s-1 m-2 in water. Azimuth: < ± 1% error over 360° at 45° elevation. Cosine Correction: Optimized for underwater and atmospheric use. (www.licor.com)

Dataset-specific Instrument NameWater Temp Sensor
Generic Instrument NameWater Temperature Sensor
Dataset-specific Descriptioncertified digital thermometer (Model 15-077-8, Fisher Scientific,±0.05 °C)
Generic Instrument DescriptionGeneral term for an instrument that measures the temperature of the water with which it is in contact (thermometer).

Dataset-specific Instrument NameHemocytometer
Generic Instrument NameHemocytometer
Generic Instrument DescriptionA hemocytometer is a small glass chamber, resembling a thick microscope slide, used for determining the number of cells per unit volume of a suspension. Originally used for performing blood cell counts, a hemocytometer can be used to count a variety of cell types in the laboratory. Also spelled as "haemocytometer". Description from: http://hlsweb.dmu.ac.uk/ahs/elearning/RITA/Haem1/Haem1.html.

Dataset-specific Instrument NameGas Analyzer
Generic Instrument NameGas Analyzer
Dataset-specific DescriptionInfra Red gas analyzer (S151, Qubit Systems)
Generic Instrument DescriptionGas Analyzers - Instruments for determining the qualitative and quantitative composition of gas mixtures.

Dataset-specific Instrument Nameoptrode
Generic Instrument NameOptode
Dataset-specific DescriptionA Ruthenium-based optrode (FOXY-R, 1.58 diameter, Ocean Optics) connected to a spectrophotometer (USB2000, Ocean Optics) and interfaced with a computer running the manufacturers software (OOISensor, version 1.00.08). The optrode was calibrated using a zero solution (0.01 M Na2B4O7·10H2O supersaturated with Na2SO3) and 100% air saturation using water-saturated air at the treatment temperature.
Generic Instrument DescriptionAn optode or optrode is an optical sensor device that optically measures a specific substance usually with the aid of a chemical transducer.

Dataset-specific Instrument NameImmersion heater
Generic Instrument NameImmersion heater
Dataset-specific Description300 Wheaters, Taikong Corporation
Generic Instrument DescriptionSubmersible heating element for water tanks and aquaria.

Dataset-specific Instrument NameAquarium chiller
Generic Instrument NameAquarium chiller
Dataset-specific DescriptionAquatech Ac11 or Shyeh Duwai Enterprise
Generic Instrument DescriptionImmersible or in-line liquid cooling device, usually with temperature control.


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Deployments

lab_Edmunds_NMMBA

Websitehttps://www.bco-dmo.org/deployment/58892
PlatformNatl Museum Mar. Bio. and Aquar. Taiwan
Start Date2010-03-18
End Date2010-03-24
DescriptionExperiments related to the research project:  'RUI- The ecophysiological basis of the response of coral larvae and early life history stages to global climate change' were conducted at the laboratories of the National Museum of Marine Biology and Aquarium in Southern Taiwan.


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Project Information

Moorea Coral Reef Long-Term Ecological Research site (MCR LTER)

Website: http://mcr.lternet.edu/
Coverage: Island of Moorea, French Polynesia

From http://www.lternet.edu/sites/mcr/ and http://mcr.lternet.edu/: The Moorea Coral Reef LTER site encompasses the coral reef complex that surrounds the island of Moorea, French Polynesia (17°30'S, 149°50'W). Moorea is a small, triangular volcanic island 20 km west of Tahiti in the Society Islands of French Polynesia. An offshore barrier reef forms a system of shallow (mean depth ~ 5-7 m), narrow (~0.8-1.5 km wide) lagoons around the 60 km perimeter of Moorea. All major coral reef types (e.g., fringing reef, lagoon patch reefs, back reef, barrier reef and fore reef) are present and accessible by small boat. The MCR LTER was established in 2004 by the US National Science Foundation (NSF) and is a partnership between the University of California Santa Barbara and California State University, Northridge. MCR researchers include marine scientists from the UC Santa Barbara, CSU Northridge, UC Davis, UC Santa Cruz, UC San Diego, CSU San Marcos, Duke University and the University of Hawaii. Field operations are conducted from the UC Berkeley Richard B. Gump South Pacific Research Station on the island of Moorea, French Polynesia. MCR LTER Data: The Moorea Coral Reef (MCR) LTER data are managed by and available directly from the MCR project data site URL shown above.  The datasets listed below were collected at or near the MCR LTER sampling locations, and funded by NSF OCE as ancillary projects related to the MCR LTER core research themes. The following publications and data resulted from this project: 2012 Edmunds PJ. Effect of pCO2 on the growth, respiration, and photophysiology of massive Porites spp. in Moorea, French Polynesia. Marine Biology 159: 2149-2160. doi:10.1594/PANGAEA.820375Porites growth_respiration_photophysDownload complete data for this publication (Excel file)

The ecophysiological basis of the response of coral larvae and early life history stages to global climate change (Climate_Coral_Larvae)

Coverage: Moorea, French Polynesia; Southern Taiwan; California State University Northridge

Tropical coral reefs face a suite of environmental assaults ranging from anchor damage to the effects of global climate change (GCC). The consequences are evident throughout the tropics, where many coral reefs have lost a substantial fraction of their coral cover in a few decades. Notwithstanding the importance of reducing the impacts of environmental stresses, the only means by which these ecosystems can recover (or simply persist) is through the recruitment of scleractinians, which is a function of successful larval development, delivery, settlement, metamorphosis, and post-settlement events. Despite wide recognition of the importance of these processes, there are few pertinent empirical data, and virtually none that address the mechanisms mediating the success of early coral life stages in a physical environmental varying at multiple spatio-temporal scales. The objective of this research is to complete one of the first comprehensive ecophysiological analyses of the early life stages of corals through a description of: (1) their functionality under 'normal' conditions, and (2) their response to the main drivers of GCC. These analyses will be completed for 2 species representative of a brooding life history strategy, and the experiments will be completed in two locations, one (Taiwan) that provides unrivalled experience in coral reproductive biology, and superb microcosm facilities, and the other (Moorea), with access to a relatively pristine environment, a well described ecological and oceanographic context (through the MCR-LTER), and the capacity to bring a strong biogeographic contrast to the project. The results of the study will be integrated through modeling to explore the effects of GCC on coral community structure over the next century. The following publications and data resulted from this project: 2013    Wall CB, Fan TY, Edmunds PJ.  Ocean acidification has no effect on thermal bleaching in the coral Seriatopora caliendrum.  Coral Reefs 33: 119-130.Symbiodinium_Seriatopora photosynthesisSymbiodinium_Seriatopora PI curveSymbiodinium_Seriatopora temp-salinity-lightSymbiodinium_Seriatopora water chemistry- Download complete data for this publication (Excel file) 2013    Wall CB, Edmunds PJ. In situ effects of low pH and elevated HCO3- on juvenile Porites spp. in Moorea, French Polynesia.  Biological Bulletin 225:92-101. Data at MCR and PANGEA: doi.pangaea.de/10.1594/PANGAEA.833913- Download complete data for this publication (Excel file) 2013    Vivian R Cumbo, Peter J Edmunds, Christopher B Wall, Tung-Yung Fan. Brooded coral larvae differ in their response to high temperature and elevated pCO2 depending on the day of release.  Marine Biology DOI 10.1007/s00227-013-2280-y. Data also at PANGEA: doi.pangaea.de/10.1594/PANGAEA.831612brooded coral larvae 2 - carbonate chemistrybrooded coral larvae 2 - larval release March 2003-2008brooded coral larvae 2 - respiration_photosyth_mortality- Download complete data for this publication (Excel file) 2013    Edmunds PJ, Cumbo VR, Fan TY. Metabolic costs of larval settlement and metamorphosis in the coral Seriatopora caliendrum under ambient and elevated pCO2.  Journal Experimental Marine Biology and Ecology 443: 33-38 Data also at PANGEA: doi:10.1594/PANGAEA.821644Coral post-settlement physiology- Download complete data for this publication (Excel file) 2013    Aaron M Dufault, Aaron Ninokawa, Lorenzo Bramanti, Vivian R Cumbo, Tung-Yung Fan, Peter J Edmunds.  The role of light in mediating the effects of ocean acidification on coral calcification.  Journal of Experimental Biology 216: 1570-1577.coral-light expt.- PARcoral-light expt.- carbonate chemistrycoral-light expt.- temp_salinitycoral-light expt.- growthcoral-light expt.- proteincoral-light expt.- survival- Download complete data for this publication (Excel file) 2012    Cumbo, VR, Fan TY, Edmunds PJ. Effects of exposure duration on the response of Pocillopora damicornis larvae to elevated temperature and high pCO2.  J Exp Mar Biol Ecol 439: 100-107. Data is also at PANGEA: doi:10.1594/PANGAEA.823582brooded coral larvae 3 - carbonate chemistrybrooded coral larvae 3 - lightbrooded coral larvae 3 - mortalitybrooded coral larvae 3 - proteinbrooded coral larvae 3 - respiration and proteinbrooded coral larvae 3 - respiration raw databrooded coral larvae 3 - symbiont densitybrooded coral larvae 3 - tank temperature- Download part 1 of data for this publication (Excel file)- Download tank parameters data for this publication (Excel file) 2012    Cumbo, VR, Fan TY, Edmunds PJ.  Physiological development of brooded larvae from two pocilloporid corals in Taiwan.  Marine Biology 159: 2853-2866.brooded coral - carbonate chemistrybrooded coral - releasebrooded coral - respirationbrooded coral - settlement competencybrooded coral - size_Julybrooded coral - size_protein_symbionts_photosynth- Download complete data for this publication (Excel file) 2012    Dufault, Aaron M; Vivian R Cumbo; Tung-Yung Fan; Peter J Edmunds.  Effects of diurnally oscillating pCO2 on the calcification and survival of coral recruits.  Royal Society of London (B) 279: 2951-2958.  doi:10.1098/rspb.2011.2545 Data is also at PANGEA: doi:10.1594/PANGAEA.830185recruit_growth_arearecruit_growth_weightrecruit_seawater_chemistryrecruit_survival- Download complete data for this publication (Excel file) 2011    Edmunds PJ, Cumbo V, Fan TY.  Effects of temperature on the respiration of brooded larvae from tropical reef corals.  Journal of Experimental Biology 214: 2783-2790. CoralLarvae_comparison_respirCoralLarvae_releaseCoralLarvae_respirCoralLarvae_size- Download complete data for this publication (Excel file)

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Program Information

Long Term Ecological Research network (LTER)

Website: http://www.lternet.edu/
Coverage: United States

adapted from http://www.lternet.edu/ The National Science Foundation established the LTER program in 1980 to support research on long-term ecological phenomena in the United States. The Long Term Ecological Research (LTER) Network is a collaborative effort involving more than 1800 scientists and students investigating ecological processes over long temporal and broad spatial scales. The LTER Network promotes synthesis and comparative research across sites and ecosystems and among other related national and international research programs. The LTER research sites represent diverse ecosystems with emphasis on different research themes, and cross-site communication, network publications, and research-planning activities are coordinated through the LTER Network Office. The coastal LTER research sites report data of interest to the research community supported by BCO-DMO (figure 1). Figure 1. This 2015 LTER research site map has been modified to highlight the coastal marine ecosystem study sites: California Current Ecosystem (CCE); Florida Coastal Everglades (FCE); Georgia Coastal Ecosystems (GCE); Plum Island Ecosystems (PIE); Santa Barbara Coastal (SBC) and Virginia Coast Reserve (VCR). Additionally, Palmer LTER studies a polar marine biome with research focused on the Antarctic pelagic marine ecosystem. Click on the map to view a larger version of the site location map. [Source: January 2015 modification of the site location map published at http://www.lternet.edu/lter-sites]

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Funding

Funding SourceAward
NSF Division of Ocean Sciences (NSF OCE) OCE-0844785

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