Protein content of brooded coral larvae at high and ambient temperature and pCO2, March 2011 (Cumbo et al, JEMBE, 2013) (MCR LTER & Climate Coral Larvae projects)
Project
Program
| Contributors | Affiliation | Role |
|---|---|---|
| Edmunds, Peter J. | California State University Northridge (CSUN) | Principal Investigator |
| Cumbo, Vivian R | California State University Northridge (CSUN) | Co-Principal Investigator |
| Fan, Tung-Yung | National Museum of Marine Biology and Aquarium (NMMBA) | Co-Principal Investigator |
| Copley, Nancy | Woods Hole Oceanographic Institution (WHOI BCO-DMO) | BCO-DMO Data Manager |
Abstract
Protein content was determined spectrophotometrically using the Bio-Rad Coomassie Blue assay in the microtiter plate protocol (BioRad Life Sciences Research, CA). At each sampling, 1 group of 8 larvae from each tub within each tank (i.e., 2 replicates tank-1) was frozen in liquid nitrogen and stored. Proteins were solubilized in 0.1 M NaOH, aided by sonication (10% amplitude for 15 s using a Branson Digital Sonifier S-250D, USA) and warming at 50 °C for 5 h. The extract was neutralized with 1 M HCl and processed in triplicate with the addition of the dye reagent. Following 30 min incubation, absorbances were measured at 595 nm using a plate reading spectrophotometer (Biotek Synergy H4 Hybrid Reader, USA), and converted to protein using a calibration prepared from bovine serum albumin. Protein content was expressed as microgram larva-1.
The 'ambient' and 'high' pCO2 levels: 49.4 Pa versus 86.2 Pa
The 'ambient' and 'high' temperatures: 24.00 °C [ambient] versus 30.49 °C
Data also available from PANGAEA: doi:10.1594/PANGAEA.823582
BCO-DMO processing notes:
- added conventional header with dataset name, PI name, version date, reference information
- renamed parameters to BCO-DMO standard
- added lab, lat, lon columns
- reduced number of significant digits
| File |
|---|
brood3_protein.csv (Comma Separated Values (.csv), 7.38 KB) MD5:5c5f4e22a8b4a02303025dac0beba966 Primary data file for dataset ID 535425 |
| File |
|---|
Biological data for "brooded coral larvae expt. 3" datasets filename: Cumbo_etal_2012_JEMBE_data1_BCODMO.xls (Octet Stream, 154.50 KB) MD5:e6c5e6012df9bfc581b9f769f7e52a98 Original biological data for Cumbo et al 2013 including respiration raw data, respiration by mg protein, symbiont densities, protein content, % mortality |
Tank physical data filename: Cumbo_etal_2012_JEMBE_Tank_Parameters_BCODMO.xlsx (Octet Stream, 57.05 KB) MD5:0170402805d7c1fe4784a51d2b26fb66 Tank physical data for "brooded coral larvae 3" experiment including seawater chemistry, light and temperature data. |
| Parameter | Description | Units |
| lab | laboratory | unitless |
| lat | latitude; north is positive | decimal degrees |
| lon | longitude; east is positive | decimal degrees |
| sample | sample identification | unitless |
| temp | target temperature | degrees Celsius |
| treatment_pCO2 | pCO2 treatment: ambient (47.5 - 49.3 Pa) or high (85.2 - 87.2 Pa) | unitless |
| days | days since start of experiment | unitless |
| conc | concentration of protein in ? | unknown |
| num_larv | number of larvae | integer |
| protein_mean | mean of ? | unknown |
| prot_stdev | standard deviation of ? | unknown |
| cv | ? | percent |
| conc_actual | actual concentration of protein in ? | unknown |
| vol | sample volume? | ml |
| total_mg_protein | total protein in 8 larvae | mg protein |
| num_larv_samp | number of larvae in sample | integer |
| mg_protein_larva | protein content per larva in milligrams | mg protein |
| umg_protein_larva | protein content per larva in micrograms | umg protein |
| Dataset-specific Instrument Name | LI-COR LI-192 light sensor |
| Generic Instrument Name | LI-COR LI-192 PAR Sensor |
| Dataset-specific Description | cosine-corrected quantum light meter (Li-Cor LI-192 attached to an LI-1400) |
| Generic Instrument Description | The LI-192 Underwater Quantum Sensor (UWQ) measures underwater or atmospheric Photon Flux Density (PPFD) (Photosynthetically Available Radiation from 360 degrees) using a Silicon Photodiode and glass filters encased in a waterproof housing. The LI-192 is cosine corrected and features corrosion resistant, rugged construction for use in freshwater or saltwater and pressures up to 800 psi (5500 kPa, 560 meters depth). Typical output is in um s-1 m-2. The LI-192 uses computer-tailored filter glass to achieve the desired quantum response. Calibration is traceable to NIST. The LI-192 serial numbers begin with UWQ-XXXXX. LI-COR has been producing Underwater Quantum Sensors since 1973.
These LI-192 sensors are typically listed as LI-192SA to designate the 2-pin connector on the base of the housing and require an Underwater Cable (LI-COR part number 2222UWB) to connect to the pins on the Sensor and connect to a data recording device.
The LI-192 differs from the LI-193 primarily in sensitivity and angular response.
193: Sensitivity: Typically 7 uA per 1000 umol s-1 m-2 in water. Azimuth: < ± 3% error over 360° at 90° from normal axis. Angular Response: < ± 4% error up to ± 90° from normal axis.
192: Sensitivity: Typically 4 uA per 1000 umol s-1 m-2 in water. Azimuth: < ± 1% error over 360° at 45° elevation. Cosine Correction: Optimized for underwater and atmospheric use.
(www.licor.com) |
| Dataset-specific Instrument Name | Water Temp Sensor |
| Generic Instrument Name | Water Temperature Sensor |
| Dataset-specific Description | certified digital thermometer (Model 15-077-8, Fisher Scientific,±0.05 °C) |
| Generic Instrument Description | General term for an instrument that measures the temperature of the water with which it is in contact (thermometer). |
| Dataset-specific Instrument Name | Hemocytometer |
| Generic Instrument Name | Hemocytometer |
| Generic Instrument Description | A hemocytometer is a small glass chamber, resembling a thick microscope slide, used for determining the number of cells per unit volume of a suspension. Originally used for performing blood cell counts, a hemocytometer can be used to count a variety of cell types in the laboratory. Also spelled as "haemocytometer". Description from:
http://hlsweb.dmu.ac.uk/ahs/elearning/RITA/Haem1/Haem1.html. |
| Dataset-specific Instrument Name | spectrophotometer |
| Generic Instrument Name | Spectrophotometer |
| Dataset-specific Description | - USB2000, Ocean Optics
- plate reading spectrophotometer (Biotek Synergy H4 Hybrid Reader, USA) |
| Generic Instrument Description | An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples. |
| Dataset-specific Instrument Name | Gas Analyzer |
| Generic Instrument Name | Gas Analyzer |
| Dataset-specific Description | Infra Red gas analyzer (S151, Qubit Systems) |
| Generic Instrument Description | Gas Analyzers - Instruments for determining the qualitative and quantitative composition of gas mixtures. |
| Dataset-specific Instrument Name | optrode |
| Generic Instrument Name | Optode |
| Dataset-specific Description | A Ruthenium-based optrode (FOXY-R, 1.58 diameter, Ocean Optics) connected to a spectrophotometer (USB2000, Ocean Optics) and interfaced with a computer running the manufacturers software (OOISensor, version 1.00.08). The optrode was calibrated using a zero solution (0.01 M Na2B4O7·10H2O supersaturated with Na2SO3) and 100% air saturation using water-saturated air at the treatment temperature. |
| Generic Instrument Description | An optode or optrode is an optical sensor device that optically measures a specific substance usually with the aid of a chemical transducer. |
| Dataset-specific Instrument Name | Immersion heater |
| Generic Instrument Name | Immersion heater |
| Dataset-specific Description | 300 Wheaters, Taikong Corporation |
| Generic Instrument Description | Submersible heating element for water tanks and aquaria. |
| Dataset-specific Instrument Name | Aquarium chiller |
| Generic Instrument Name | Aquarium chiller |
| Dataset-specific Description | Aquatech Ac11 or Shyeh Duwai Enterprise |
| Generic Instrument Description | Immersible or in-line liquid cooling device, usually with temperature control. |
| Dataset-specific Instrument Name | sonicator |
| Generic Instrument Name | ultrasonic cell disrupter (sonicator) |
| Dataset-specific Description | Branson Digital Sonifier |
| Generic Instrument Description | Instrument that applies sound energy to agitate particles in a sample. |
| Dataset-specific Instrument Name | plate reader |
| Generic Instrument Name | plate reader |
| Dataset-specific Description | BioRad Life Sciences Research, CA |
| Generic Instrument Description | Plate readers (also known as microplate readers) are laboratory instruments designed to detect biological, chemical or physical events of samples in microtiter plates. They are widely used in research, drug discovery, bioassay validation, quality control and manufacturing processes in the pharmaceutical and biotechnological industry and academic organizations. Sample reactions can be assayed in 6-1536 well format microtiter plates. The most common microplate format used in academic research laboratories or clinical diagnostic laboratories is 96-well (8 by 12 matrix) with a typical reaction volume between 100 and 200 uL per well. Higher density microplates (384- or 1536-well microplates) are typically used for screening applications, when throughput (number of samples per day processed) and assay cost per sample become critical parameters, with a typical assay volume between 5 and 50 µL per well. Common detection modes for microplate assays are absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, and fluorescence polarization. From: http://en.wikipedia.org/wiki/Plate_reader, 2014-09-0-23. |
lab_Edmunds_NMMBA
| Website | |
| Platform | Natl Museum Mar. Bio. and Aquar. Taiwan |
| Start Date | 2010-03-18 |
| End Date | 2010-03-24 |
| Description | Experiments related to the research project: 'RUI- The ecophysiological basis of the response of coral larvae and early life history stages to global climate change' were conducted at the laboratories of the National Museum of Marine Biology and Aquarium in Southern Taiwan. |
Moorea Coral Reef Long-Term Ecological Research site (MCR LTER)
From http://www.lternet.edu/sites/mcr/ and http://mcr.lternet.edu/:
The Moorea Coral Reef LTER site encompasses the coral reef complex that surrounds the island of Moorea, French Polynesia (17°30'S, 149°50'W). Moorea is a small, triangular volcanic island 20 km west of Tahiti in the Society Islands of French Polynesia. An offshore barrier reef forms a system of shallow (mean depth ~ 5-7 m), narrow (~0.8-1.5 km wide) lagoons around the 60 km perimeter of Moorea. All major coral reef types (e.g., fringing reef, lagoon patch reefs, back reef, barrier reef and fore reef) are present and accessible by small boat.
The MCR LTER was established in 2004 by the US National Science Foundation (NSF) and is a partnership between the University of California Santa Barbara and California State University, Northridge. MCR researchers include marine scientists from the UC Santa Barbara, CSU Northridge, UC Davis, UC Santa Cruz, UC San Diego, CSU San Marcos, Duke University and the University of Hawaii. Field operations are conducted from the UC Berkeley Richard B. Gump South Pacific Research Station on the island of Moorea, French Polynesia.
MCR LTER Data: The Moorea Coral Reef (MCR) LTER data are managed by and available directly from the MCR project data site URL shown above. The datasets listed below were collected at or near the MCR LTER sampling locations, and funded by NSF OCE as ancillary projects related to the MCR LTER core research themes.
This project is supported by continuing grants with slight name variations:
LTER: Long-Term Dynamics of a Coral Reef Ecosystem
LTER: MCR II - Long-Term Dynamics of a Coral Reef Ecosystem
LTER: MCR IIB: Long-Term Dynamics of a Coral Reef Ecosystem
LTER: MCR III: Long-Term Dynamics of a Coral Reef Ecosystem
LTER: MCR IV: Long-Term Dynamics of a Coral Reef Ecosystem
The ecophysiological basis of the response of coral larvae and early life history stages to global climate change (Climate_Coral_Larvae)
Tropical coral reefs face a suite of environmental assaults ranging from anchor damage to the effects of global climate change (GCC). The consequences are evident throughout the tropics, where many coral reefs have lost a substantial fraction of their coral cover in a few decades. Notwithstanding the importance of reducing the impacts of environmental stresses, the only means by which these ecosystems can recover (or simply persist) is through the recruitment of scleractinians, which is a function of successful larval development, delivery, settlement, metamorphosis, and post-settlement events. Despite wide recognition of the importance of these processes, there are few pertinent empirical data, and virtually none that address the mechanisms mediating the success of early coral life stages in a physical environmental varying at multiple spatio-temporal scales.
The objective of this research is to complete one of the first comprehensive ecophysiological analyses of the early life stages of corals through a description of: (1) their functionality under 'normal' conditions, and (2) their response to the main drivers of GCC. These analyses will be completed for 2 species representative of a brooding life history strategy, and the experiments will be completed in two locations, one (Taiwan) that provides unrivalled experience in coral reproductive biology, and superb microcosm facilities, and the other (Moorea), with access to a relatively pristine environment, a well described ecological and oceanographic context (through the MCR-LTER), and the capacity to bring a strong biogeographic contrast to the project. The results of the study will be integrated through modeling to explore the effects of GCC on coral community structure over the next century.
The following publications and data resulted from this project:
2013 Wall CB, Fan TY, Edmunds PJ. Ocean acidification has no effect on thermal bleaching in the coral Seriatopora caliendrum. Coral Reefs 33: 119-130.
Symbiodinium_Seriatopora photosynthesis
Symbiodinium_Seriatopora PI curve
Symbiodinium_Seriatopora temp-salinity-light
Symbiodinium_Seriatopora water chemistry
- Download complete data for this publication (Excel file)
2013 Wall CB, Edmunds PJ. In situ effects of low pH and elevated HCO3- on juvenile Porites spp. in Moorea, French Polynesia. Biological Bulletin 225:92-101.
Data at MCR and PANGEA: doi.pangaea.de/10.1594/PANGAEA.833913
- Download complete data for this publication (Excel file)
2013 Vivian R Cumbo, Peter J Edmunds, Christopher B Wall, Tung-Yung Fan. Brooded coral larvae differ in their response to high temperature and elevated pCO2 depending on the day of release. Marine Biology DOI 10.1007/s00227-013-2280-y.
Data also at PANGEA: doi.pangaea.de/10.1594/PANGAEA.831612
brooded coral larvae 2 - carbonate chemistry
brooded coral larvae 2 - larval release March 2003-2008
brooded coral larvae 2 - respiration_photosyth_mortality
- Download complete data for this publication (Excel file)
2013 Edmunds PJ, Cumbo VR, Fan TY. Metabolic costs of larval settlement and metamorphosis in the coral Seriatopora caliendrum under ambient and elevated pCO2. Journal Experimental Marine Biology and Ecology 443: 33-38 Data also at PANGEA: doi:10.1594/PANGAEA.821644
Coral post-settlement physiology
- Download complete data for this publication (Excel file)
2013 Aaron M Dufault, Aaron Ninokawa, Lorenzo Bramanti, Vivian R Cumbo, Tung-Yung Fan, Peter J Edmunds. The role of light in mediating the effects of ocean acidification on coral calcification. Journal of Experimental Biology 216: 1570-1577.
coral-light expt.- PAR
coral-light expt.- carbonate chemistry
coral-light expt.- temp_salinity
coral-light expt.- growth
coral-light expt.- protein
coral-light expt.- survival
- Download complete data for this publication (Excel file)
2012 Cumbo, VR, Fan TY, Edmunds PJ. Effects of exposure duration on the response of Pocillopora damicornis larvae to elevated temperature and high pCO2. J Exp Mar Biol Ecol 439: 100-107.
Data is also at PANGEA: doi:10.1594/PANGAEA.823582
brooded coral larvae 3 - carbonate chemistry
brooded coral larvae 3 - light
brooded coral larvae 3 - mortality
brooded coral larvae 3 - protein
brooded coral larvae 3 - respiration and protein
brooded coral larvae 3 - respiration raw data
brooded coral larvae 3 - symbiont density
brooded coral larvae 3 - tank temperature
- Download part 1 of data for this publication (Excel file)
- Download tank parameters data for this publication (Excel file)
2012 Cumbo, VR, Fan TY, Edmunds PJ. Physiological development of brooded larvae from two pocilloporid corals in Taiwan. Marine Biology 159: 2853-2866.
brooded coral - carbonate chemistry
brooded coral - release
brooded coral - respiration
brooded coral - settlement competency
brooded coral - size_July
brooded coral - size_protein_symbionts_photosynth
- Download complete data for this publication (Excel file)
2012 Dufault, Aaron M; Vivian R Cumbo; Tung-Yung Fan; Peter J Edmunds. Effects of diurnally oscillating pCO2 on the calcification and survival of coral recruits. Royal Society of London (B) 279: 2951-2958. doi:10.1098/rspb.2011.2545
Data is also at PANGEA: doi:10.1594/PANGAEA.830185
recruit_growth_area
recruit_growth_weight
recruit_seawater_chemistry
recruit_survival
- Download complete data for this publication (Excel file)
2011 Edmunds PJ, Cumbo V, Fan TY. Effects of temperature on the respiration of brooded larvae from tropical reef corals. Journal of Experimental Biology 214: 2783-2790.
CoralLarvae_comparison_respir
CoralLarvae_release
CoralLarvae_respir
CoralLarvae_size
- Download complete data for this publication (Excel file)
Long Term Ecological Research network (LTER)
adapted from http://www.lternet.edu/
The National Science Foundation established the LTER program in 1980 to support research on long-term ecological phenomena in the United States. The Long Term Ecological Research (LTER) Network is a collaborative effort involving more than 1800 scientists and students investigating ecological processes over long temporal and broad spatial scales. The LTER Network promotes synthesis and comparative research across sites and ecosystems and among other related national and international research programs. The LTER research sites represent diverse ecosystems with emphasis on different research themes, and cross-site communication, network publications, and research-planning activities are coordinated through the LTER Network Office.
2017 LTER research site map obtained from https://lternet.edu/site/lter-network/
| Funding Source | Award |
|---|---|
| NSF Division of Ocean Sciences (NSF OCE) |
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