http://lod.bco-dmo.org/id/dataset/540954 eng; USA utf8 dataset Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2014-11-25 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Copper data with 5 uM SA lingand from Hood Canal, Puget Sound, Washington, USA from R/V Clifford A. Barnes CB960, CB974, CB980, CB985, 2011-2012 (Nitrification and Marine Planktonic Biodiversity project) 2014-11-17 publication 2014-11-17 revision BCO-DMO Linked Data URI 2014-11-17 creation http://lod.bco-dmo.org/id/dataset/540954 James W. Moffett University of Southern California principalInvestigator Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. publisher Cite this dataset as: Moffett, J. W. (2014) Copper data with 5 uM SA lingand from Hood Canal, Puget Sound, Washington, USA from R/V Clifford A. Barnes CB960, CB974, CB980, CB985, 2011-2012 (Nitrification and Marine Planktonic Biodiversity project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2014-11-17) Version Date 2014-11-17 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/540954 [access date] Copper data with 5 uM SA lingand from Hood Canal, Puget Sound, Washington, USA Dataset Description: &lt;p&gt;Water samples were collected at various depths from the Hood Canal and the Cu2 + concentrations, organic ligand (L) concentrations and their corresponding conditional stability constants (K) were characterized using a competitive ligand exchange adsorptive cathodic stripping voltammetry (CLE-ACSV) adapted from Jacquot et al. (2013) and Moffett and Dupont (2007) with salicylaldoxime (SA) as the competing ligand.&lt;/p&gt; Methods and Sampling: &lt;p&gt;All samples for total dissolved copper on this cruise were well above our detection limit of 100pM determined from the standard deviation of procedural blanks using our ICP-MS methods.&lt;/p&gt; &lt;p&gt;Cu speciation was determined using cathodic stripping voltammetry on samples titrated with added copper.&lt;/p&gt; Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1046098 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1046098 onGoing James W. Moffett University of Southern California 213-740-5626 Biological Sciences, Earth Sciences, and Civil and Environmental Engineering 3616 Trousdale Pkwy Los Angeles CA 90089 USA jmoffett@usc.edu pointOfContact asNeeded Dataset Version: 2014-11-17 Unknown BASI CGME CTD SBE 911plus GO-FLO ICP Mass Spec Voltammetry Analyzers theme None, User defined BASi Controlled Growth Mercury Electrode CTD Sea-Bird SBE 911plus GO-FLO Bottle Inductively Coupled Plasma Mass Spectrometer Voltammetry Analyzers instrument BCO-DMO Standard Instruments CB960 CB974 CB980 CB985 service Deployment Activity eastern N. Pacific place Locations otherRestrictions otherRestrictions Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use. Dimensions of Biodiversity http://www.nsf.gov/funding/pgm_summ.jsp?pims_id=503446 Dimensions of Biodiversity (adapted from the NSF Synopsis of Program) Dimensions of Biodiversity is a program solicitation from the NSF Directorate for Biological Sciences. FY 2010 was year one of the program.  [MORE from NSF] The NSF Dimensions of Biodiversity program seeks to characterize biodiversity on Earth by using integrative, innovative approaches to fill rapidly the most substantial gaps in our understanding. The program will take a broad view of biodiversity, and in its initial phase will focus on the integration of genetic, taxonomic, and functional dimensions of biodiversity. Project investigators are encouraged to integrate these three dimensions to understand the interactions and feedbacks among them. While this focus complements several core NSF programs, it differs by requiring that multiple dimensions of biodiversity be addressed simultaneously, to understand the roles of biodiversity in critical ecological and evolutionary processes. Dimensions of Biodiversity largerWorkCitation program Significance of nitrification in shaping planktonic biodiversity in the ocean https://osprey.bco-dmo.org/project/2228 Significance of nitrification in shaping planktonic biodiversity in the ocean <p>Microorganisms sustain the biogeochemical cycling of nitrogen, one of the most important nutrient cycles on earth. A key step in this cycle, the oxidation of ammonia to nitrite by autotrophic microorganisms, was for a century thought mediated by a few restricted bacterial genera. Significant ammonia oxidation, perhaps most, is now attributed to a previously enigmatic group of Archaea - the ammonia-oxidizing archaea (AOA) - of high abundance in both marine and terrestrial environments. The investigators prior physiological and environmental analyses, the foundation for this proposal, have shown that AOA are active within the marine photic zone and that their competitive fitness in the marine environment is at least in part attributable to an extremely high affinity for ammonia, growing at near maximum growth rates at concentrations of ammonia that would not sustain known bacterial ammonia oxidizers, and an unusual copper-based respiratory system that may render them more competitive in iron limited environments. The compelling inference from these prior analyses is that AOA alter and possibly control the forms of fixed nitrogen available to other microbial assemblages within the photic zone by converting ammonia, a nearly universally available form of nitrogen, into nitrite, a form only available to nitrite oxidizing bacteria and some phytoplankton. If correct, this has a significant impact on biodiversity.</p> <p>The PIs will use the most recent technological advances in protein and high throughput sequencing to evaluate the significance of nitrification in shaping biodiversity (genomic and metagenomics), activity (transcriptome, proteome and stable isotope probing), and in controlling availability of an important trace element (copper). In turn, by resolving the environmental and biotic variables that influence the diversity, distribution and activity of AOA, they will advance general understanding of their taxonomy. More directly, functional knowledge of the contribution of AOA to regenerated nitrate will improve estimates of new ocean production ("biological pump") based on nitrate assimilation, which in the past has mostly neglected the importance of nitrification as a major source of nitrate. Together these studies will transform understanding of the marine nitrogen cycle, estimates of new production, and will ultimately provide a better understanding of the impact of human activity on this critical nutrient cycle.</p> <p>The nitrogen cycle has been profoundly affected by anthropogenic inputs of reactive nitrogen into terrestrial, marine, and atmospheric systems having, or predicted to have, major impacts on marine biological production, increased N20 emissions, nitrogen pollution, and eutrophication. Likewise, there is a poor understanding of the relationship between nitrogen cycling and productivity in marine ecosystems. Marine systems are increasingly affected by ocean acidification and by atmospheric inputs of reactive nitrogen. Since both changes greatly alter nitrogen available to microorganisms, the characterization of the response of these environmentally relevant AOA is of tremendous relevance to understanding the affect of acidification and anthropogenic nitrogen inputs on major ocean processes.</p> <p>The proposed project encompasses and integrates the three dimensions (functional genetic, and taxonomic) of biodiversity. First, the project is framed by function: microbial control of one of the most important nutrient cycles on earth, the nitrogen-cycle. Second, it is motivated by recent genetic analyses that associate activities of a novel clade of Archaea (provisionally assigned to a new kingdom within the Archaea, the Thaumarchaeota) with control of ammonia oxidation in the ocean. Third, it is built upon a compelling synthesis of physiological and environmental data that lead to its central hypothesis that by altering and possibly controlling the form of nitrogen, the AOA also alter biodiversity and ecological function in one of the most productive environments on earth. It identifies a specific taxonomic imperative. The tremendous genetic diversity among the globally abundant AOA catalogued almost exclusively by gene sequencing surveys and therefore lacking formal description makes it essential to resolve membership into ecologically relevant groups or clades as a prelude to developing a formal taxonomy. The investigators have assembled a group of researchers with specific expertise in each of dimension and uniquely qualified to address the research objectives outlined in an integrative way.</p> Nitrification and Marine Planktonic Biodiversity largerWorkCitation project eng; USA oceans eastern N. Pacific 2014-11-17 0 BCO-DMO catalogue of parameters from Copper data with 5 uM SA lingand from Hood Canal, Puget Sound, Washington, USA from R/V Clifford A. Barnes CB960, CB974, CB980, CB985, 2011-2012 (Nitrification and Marine Planktonic Biodiversity project) Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact https://osprey.bco-dmo.org/dataset/540954/data/download download onLine dataset &lt;p&gt;All samples for total dissolved copper on this cruise were well above our detection limit of 100pM determined from the standard deviation of procedural blanks using our ICP-MS methods.&lt;/p&gt; &lt;p&gt;Cu speciation was determined using cathodic stripping voltammetry on samples titrated with added copper.&lt;/p&gt; Specified by the Principal Investigator(s) &lt;p&gt;Data points below the detection limit of the cathodic stripping voltammetry technique (~ 0.15 nM) were not used in the titration. However, all titrations had a significant number of points to fit a non-linear regression curve. In instances where the fit did not converged, or converged at a non-realistic value (e.g. negative numbers) the data were flagged.&lt;/p&gt; &lt;p&gt;The standard error for [Cu2+] is asymmetric because it is not determined the same way - hence the different values.&lt;/p&gt; &lt;p&gt;The Cu2+ error propagation was derived from the standard deviations of K and [L] when used in equation 11 from Moffett and Dupont 2007 to calculate [Cu2+].&lt;/p&gt; Specified by the Principal Investigator(s) asNeeded 7.x-1.1 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact BASI CGME BASI CGME PI Supplied Instrument Name: BASI CGME PI Supplied Instrument Description:BioAnalytical Systems (BASi) Controlled Growth Mercury Electrode set to the Static Mercury Drop setting (drop size: 14) and interfaced with a BASi Epsilon ε2 voltammetric analyzer. Instrument Name: BASi Controlled Growth Mercury Electrode Instrument Short Name:BASI CGME Instrument Description: Bioanalytical Systems (BASi) Mercury drop electrodes are generated by the BASi Controlled Growth Mercury Electrode (CGME) in three modes: DME (Dropping Mercury Electrode) - mercury is allowed to flow freely from the reservoir down the capillary and so the growth of the mercury drop and its lifetime is controlled by gravity. (The optional 100 um capillary is recommended for this mode.) SMDE (Static Mercury Drop Electrode) - the drop size is determined by the length of time for which the fast-response capillary valve is opened, and the drop is dislodged by a drop knocker. The dispense/knock timing is microprocessor-controlled and is typically coordinated with the potential pulse or square-wave waveform. This mode can also used to generate the Hanging Mercury Drop Electrode required for stripping experiments. CGME (Controlled Growth Mercury Electrode) - the mercury drop is grown by a series of pulses that open the capillary valve. The number of pulses, their duration, and their frequency can be varied by PC control, providing great flexibility in both the drop size and its rate of growth. This CGME mode can be used for both polarographic and stripping experiments. http://www.basinc.com/products/ec/cgme.php CTD SBE 911plus CTD SBE 911plus PI Supplied Instrument Name: CTD SBE 911plus Instrument Name: CTD Sea-Bird SBE 911plus Instrument Short Name:CTD SBE 911plus Instrument Description: The Sea-Bird SBE 911 plus is a type of CTD instrument package for continuous measurement of conductivity, temperature and pressure. The SBE 911 plus includes the SBE 9plus Underwater Unit and the SBE 11plus Deck Unit (for real-time readout using conductive wire) for deployment from a vessel. The combination of the SBE 9 plus and SBE 11 plus is called a SBE 911 plus. The SBE 9 plus uses Sea-Bird's standard modular temperature and conductivity sensors (SBE 3 plus and SBE 4). The SBE 9 plus CTD can be configured with up to eight auxiliary sensors to measure other parameters including dissolved oxygen, pH, turbidity, fluorescence, light (PAR), light transmission, etc.). more information from Sea-Bird Electronics Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0058/ GO-FLO GO-FLO PI Supplied Instrument Name: GO-FLO PI Supplied Instrument Description:10 L Teflon-coated Go-Flo bottles (General Oceanics) attached to Kevlar wire Instrument Name: GO-FLO Bottle Instrument Short Name:GO-FLO Instrument Description: GO-FLO bottle cast used to collect water samples for pigment, nutrient, plankton, etc. The GO-FLO sampling bottle is specially designed to avoid sample contamination at the surface, internal spring contamination, loss of sample on deck (internal seals), and exchange of water from different depths. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/30/ ICP Mass Spec ICP Mass Spec PI Supplied Instrument Name: ICP Mass Spec PI Supplied Instrument Description:Finnegan Element 2 (Thermo Scientific) Instrument Name: Inductively Coupled Plasma Mass Spectrometer Instrument Short Name:ICP Mass Spec Instrument Description: An ICP Mass Spec is an instrument that passes nebulized samples into an inductively-coupled gas plasma (8-10000 K) where they are atomized and ionized. Ions of specific mass-to-charge ratios are quantified in a quadrupole mass spectrometer. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB15/ Voltammetry Analyzers Voltammetry Analyzers PI Supplied Instrument Name: Voltammetry Analyzers PI Supplied Instrument Description:BASi Epsilon ε2 voltammetric analyzer Instrument Name: Voltammetry Analyzers Instrument Short Name:Voltammetry Analyzers Instrument Description: Instruments that obtain information about an analyte by applying a potential and measuring the current produced in the analyte. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB35/ Cruise: CB960 CB960 R/V Clifford A. Barnes Community Standard Description International Council for the Exploration of the Sea R/V Clifford A. Barnes vessel CB960 Allan Devol University of Washington Cruise: CB974 CB974 R/V Clifford A. Barnes Community Standard Description International Council for the Exploration of the Sea R/V Clifford A. Barnes vessel CB974 Anitra E. Ingalls University of Washington Cruise: CB980 CB980 R/V Clifford A. Barnes Community Standard Description International Council for the Exploration of the Sea R/V Clifford A. Barnes vessel CB980 Anitra E. Ingalls University of Washington Cruise: CB985 CB985 R/V Clifford A. Barnes Community Standard Description International Council for the Exploration of the Sea R/V Clifford A. Barnes vessel CB985 Anitra E. Ingalls University of Washington R/V Clifford A. Barnes Community Standard Description International Council for the Exploration of the Sea R/V Clifford A. Barnes vessel