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&lt;p&gt;1. What phylogeographic breaks occur in the system? It is important to distinguish between phylogeographic history and connectivity. A phylogeographic break with no shared alleles between populations implies a long history of isolation or possibly cryptic speciation.&lt;/p&gt;
&lt;p&gt;2. Are populations connected by ongoing migration? This is the fundamental question about connectivity and the scale of genetic variation in marine species with planktonic larvae.&lt;/p&gt;
&lt;p&gt;3. What biophysical processes underlie observed connectivities? Biological processes (e.g., larval distributions in the water column, timing of reproduction, and planktonic larval duration) and physical processes of transport and dispersion interact to determine connectivity.&lt;/p&gt;
&lt;p&gt;The oceanographic model for the IAS will be improved and coupled to a Lagrangian larval transport model. The field program includes time-series sampling of larvae at seeps with records of current velocities, water column sampling to determine larval distribution potential, shipboard studies of larval biology and behavior, and sampling of benthic target species. Phylogenetic and population genetic tools will be used to explore historical and contemporary gene flow. Iterative interactions among the science teams will advance our understanding of connectivity in the deep sea and to develop effective and best methods for hypothesis testing under the constraints of working in a relatively inaccessible environment. Since their discovery, deep-sea chemosynthetic ecosystems have been novel systems within which to test the generality of paradigms developed for shallow-water species. This study will explore scale-dependent biodiversity and recruitment dynamics in deep-sea seep communities, and will identify key factors underlying population persistence and maintenance of biodiversity in these patchy systems.&lt;/p&gt;
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