http://lod.bco-dmo.org/id/dataset/544898
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2015-01-15
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Antibacterial assays of Montipora capitata mucus collected in Kaneohe Bay, Oahu, Hawaii during 2014
2014-08-28
publication
2014-08-28
revision
BCO-DMO Linked Data URI
2014-08-28
creation
http://lod.bco-dmo.org/id/dataset/544898
Deborah J. Gochfeld
University of Mississippi
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Gochfeld, D. J. (2014) Antibacterial assays of Montipora capitata mucus collected in Kaneohe Bay, Oahu, Hawaii during 2014. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2014-08-28) Version Date 2014-08-28 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/544898 [access date]
Antibacterial assays of Montipora capitata mucus Dataset Description: <p>Data access is restricted. Please contact the PI for further information.</p>
<p><strong>Related datasets:</strong></p>
<p><a href="http://www.bco-dmo.org/dataset/544870">Montipora antibacterial-aqueous</a><br />
<a href="http://www.bco-dmo.org/dataset/544862">Montipora antibacterial-organic</a><br />
<a href="http://www.bco-dmo.org/dataset/544904">Montipora chemical fingerprints</a><br />
<a href="http://www.bco-dmo.org/dataset/545991">MWS accession numbers</a><br />
<a href="http://www.bco-dmo.org/dataset/544910">MWS lesion progression</a></p>
<p><strong>Related references:</strong></p>
<p>Deborah J. Gochfeld, Greta S. Aeby. 2008.Antibacterial chemical defenses in Hawaiian corals provide possible protection from disease. Mar Ecol Prog Ser 362: 119-128. doi: 10.3354/meps07418</p> Methods and Sampling: <p><strong>Mucus sampling:</strong>&nbsp; Mucus was obtained from the surfaces of replicate orange and red colonies of Montipora capitata collected in Kaneohe Bay, Oahu, using a sterile pipettor and frozen immediately.</p>
<p><strong>Bacterial strains tested:</strong> &nbsp;The strains used for the antibacterial assays were selected as model systems to represent a range of potential bacterial pathogens from the marine environment, including known coral pathogens (Aurantimonas coralicida, Serratia marcescens, Vibrio coraliilyticus, Vibrio shiloi) and human enteric bacteria that have the potential to enter near-shore waters and can survive in the marine environment (Yersinia enterocolitica), and strains previously isolated from the surfaces of Hawaiian corals (Klebsiella pneumonia, Vibrio agarivorans) (Gochfeld &amp; Aeby 2008). &nbsp;In addition, bacterial strains isolated from surfaces of Montipora capitata (OCN001, OCN002, OCN003, OCN008, O1-1, O2-8, R1-13, R5-5, R5-29) by Dr. Sean Callahan’s lab at University of Hawaii were also used. &nbsp;Two of these, OCN002 and OCN008, are pathogens associated with Montipora White Syndrome (Ushijima et al. 2012, 2014).</p>
<p><strong>Growth inhibition assay</strong> (Gochfeld &amp; Aeby 2008):&nbsp; Assays were run in triplicate in 96 well plates. Plates contained 3 wells of bacteria + each mucus sample (n = 5 replicate corals of 2 color morphs), along with the following controls:&nbsp; bacteria only, media only, and mucus solution to control for the coloration of extracts.&nbsp; Twenty-four hour bacterial cultures in exponential growth were diluted to optical density (OD) 600, and 100 μl were added to each well. 10 μl of mucus were added to the experimental wells. Wells were mixed by carefully pipetting their contents, and an initial reading (time 0) of OD was made on a BioTek Synergy™ HT Multi-Detection Microplate Reader. Plates were then covered with foil and incubated on a shaker for 24 h and a final reading was made. Prior to each reading, wells were mixed again to resuspend any settled material.&nbsp;</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0961384 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0961384
completed
Deborah J. Gochfeld
University of Mississippi
662-915-6769
National Center for Natural Products Research P.O. Box 1848
University
MS
38677
USA
gochfeld@olemiss.edu
pointOfContact
asNeeded
Dataset Version: 2014-08-28
Unknown
assay
color_morph
coral
Aurantimonas_coralicida
Klebsiella_pneumoniae
Pseudomonas_nautica
Serratia_marcescens
Vibrio_agarivorans
Vibrio_corallyliticus
Vibrio_shiloi
Yersinia_enterocolitica
OC_N001
OC_N002
OC_N003
OC_N008
O1_1
O2_8
O2_12
R1_13
R5_5
R5_13
R5_29
plate reader
theme
None, User defined
No BCO-DMO term
sample identification
featureType
BCO-DMO Standard Parameters
plate reader
instrument
BCO-DMO Standard Instruments
Aeby_2014
service
Deployment Activity
Kaneohe Bay, Oahu, Hawaii
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Host-environment-pathogen interactions in a model coral disease system
https://osprey.bco-dmo.org/project/525619
Host-environment-pathogen interactions in a model coral disease system
<p><em>Extracted from the NSF award abstract:</em></p>
<p>Diseases of marine organisms have emerged as a serious problem contributing to the decline of coral reef resources worldwide. Loss of coral reef habitats carry social and economic implications especially in island states, such as Hawaii, which depend on reefs for food, shoreline protection and tourism. Our ability to manage coral diseases is hampered by a lack of knowledge of which environmental variables affect disease, mechanisms of host defense, and the etiology of most of the numerous described coral diseases. The PIs of this project discovered a coral disease system that can be used as a model to explore many components of the host-environment-pathogen triangle of disease causation. Montipora white syndrome (MWS) is an infectious disease that results in progressive tissue loss on colonies of Montipora capitata, and has been found on reefs throughout the Hawaiian archipelago. It is particularly prevalent in Kaneohe Bay, Oahu, which has a long history of reduced water quality, and this suboptimal environment sets the stage where host-pathogen interactions occur. In Kaneohe Bay, M. capitata is a major reef-building species, and is found in two color morphs (red and orange) that harbor different clades of zooxanthellae. During preliminary surveys, the PIs discovered intraspecific variability in response to MWS between color morphs. Although the red morph was dominant within survey transects (80% of the colonies), the orange morph was disproportionately affected by MWS (70% of the affected colonies). Microbial studies found a shift in bacterial communities on MWS-affected and healthy M. capitata and allowed identification of potential pathogens. Numerous bacterial strains were cultured and screened for pathogenicity and three strains, which produced lesions, were identified as potential pathogens. Two of the putative pathogens (Vibrio spp.) produced diffuse tissue whereas the other bacterial strain (Pseudoalteromonas sp.) produced acute tissue loss.</p>
<p>In the field, the PIs also observed two patterns of tissue loss on M. capitata; a slow, chronic pattern of tissue loss, which they followed through time with tagged colonies (chronic MWS), but also a rapid onset of acute tissue loss (acute MWS). Thus they discovered an infectious coral disease that results in significant coral mortality that has the unique component of differences in disease susceptibility among color morphs. The PIs identified three potential bacterial pathogens that will be used to investigate underlying factors affecting the coral-environment-pathogen triad of disease causation. The Hawaii Institute of Marine Biology (HIMB) is located within Kaneohe Bay allowing year-round access to reefs for research on Montipora white syndrome. The goal of this project is to investigate the host- environment-pathogen triangle of disease causation for Montipora white syndrome. The objectives of this research will be to: 1) investigate mechanisms contributing to differential disease resistance in red (less susceptible) vs. orange (more susceptible) morphs of M. capitata. The PIs will compare antimicrobial activity in the holobiont, mucus and mucus-associated bacteria of the two color morphs of M. capitata, and will compare the natural coral-associated microbial flora between the two color morphs; 2) use manipulative aquarium studies to determine whether environmental stressors (elevated temperature, nutrient stress) differentially affect the progression or transmission efficiency of MWS in red vs. orange morphs of M. capitata; 3) use challenge experiments to confirm the role of bacterial pathogens as causative agents of MWS, and to determine the response of red vs. orange morphs of M. capitata to three putative pathogens. This project will involve a multidisciplinary team to provide a broader perspective of coral disease processes. This will be the first comprehensive study conducted on a coral disease in Hawaii.</p>
<p><em>Related Publications:</em></p>
<p>Ushijima, B, Videau, P, Burger, A, Shore-Maggio, A, Runyon, C, Sudek, M, Aeby, G and S. Callahan. 2014. Vibrio coralliilyticus strain OCN008 is an etiological agent of acute Montipora white syndrome. Applied & Environ Microbiology doi:10.1128/AEM.03463-13.</p>
<p>Ushijima B, Videau P, Aeby GS, Callahan SM. 2013. Draft Genome Sequence of Vibrio coralliilyticus Strain OCN008, Isolated from Kane'ohe Bay, Hawai'i. Genome Announc. 2013 Oct 3;1(5). doi:pii: e00786-13. 10.1128/genomeA.00786-13. PMID: 24092784</p>
<p>Ushijima B, Smith A, Aeby GS, Callahan SM (2012) Vibrio owensii Induces the Tissue Loss Disease Montipora White Syndrome in the Hawaiian Reef Coral Montipora capitata. PLoS ONE 7: e46717.</p>
coral-pathogen interaction
largerWorkCitation
project
eng; USA
biota
oceans
Kaneohe Bay, Oahu, Hawaii
2014-08-28
Kaneohe Bay, Oahu, Hawaii (21 26' N, 157 47' W)
0
BCO-DMO catalogue of parameters from Antibacterial assays of Montipora capitata mucus collected in Kaneohe Bay, Oahu, Hawaii during 2014
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/545945.rdf
Name: assay
Units: unitless
Description: <p>coral extract material examined</p>
http://lod.bco-dmo.org/id/dataset-parameter/545946.rdf
Name: color_morph
Units: unitless
Description: <p>color morph of coral</p>
http://lod.bco-dmo.org/id/dataset-parameter/545947.rdf
Name: coral
Units: unitless
Description: <p>coral fragment identification</p>
http://lod.bco-dmo.org/id/dataset-parameter/545948.rdf
Name: Aurantimonas_coralicida
Units: millimeters
Description: <p>mean zone of inhibition against Aurantimonas coralicida</p>
http://lod.bco-dmo.org/id/dataset-parameter/545949.rdf
Name: Klebsiella_pneumoniae
Units: millimeters
Description: <p>mean zone of inhibition against Klebsiella pneumoniae</p>
http://lod.bco-dmo.org/id/dataset-parameter/545950.rdf
Name: Pseudomonas_nautica
Units: millimeters
Description: <p>mean zone of inhibition against Pseudomonas nautica</p>
http://lod.bco-dmo.org/id/dataset-parameter/545951.rdf
Name: Serratia_marcescens
Units: millimeters
Description: <p>mean zone of inhibition against Serratia marcescens</p>
http://lod.bco-dmo.org/id/dataset-parameter/545952.rdf
Name: Vibrio_agarivorans
Units: millimeters
Description: <p>mean zone of inhibition against Vibrio agarivorans</p>
http://lod.bco-dmo.org/id/dataset-parameter/545953.rdf
Name: Vibrio_corallyliticus
Units: millimeters
Description: <p>mean zone of inhibition against Vibrio corallyliticus</p>
http://lod.bco-dmo.org/id/dataset-parameter/545954.rdf
Name: Vibrio_shiloi
Units: millimeters
Description: <p>mean zone of inhibition against Vibrio shiloi</p>
http://lod.bco-dmo.org/id/dataset-parameter/545955.rdf
Name: Yersinia_enterocolitica
Units: millimeters
Description: <p>mean zone of inhibition against Yersinia enterocolitica</p>
http://lod.bco-dmo.org/id/dataset-parameter/545956.rdf
Name: OC_N001
Units: millimeters
Description: <p>mean zone of inhibition against OC-N001</p>
http://lod.bco-dmo.org/id/dataset-parameter/545957.rdf
Name: OC_N002
Units: millimeters
Description: <p>mean zone of inhibition against OC-N002</p>
http://lod.bco-dmo.org/id/dataset-parameter/545958.rdf
Name: OC_N003
Units: millimeters
Description: <p>mean zone of inhibition against OC-N003</p>
http://lod.bco-dmo.org/id/dataset-parameter/545959.rdf
Name: OC_N008
Units: millimeters
Description: <p>mean zone of inhibition against OC-N008</p>
http://lod.bco-dmo.org/id/dataset-parameter/545960.rdf
Name: O1_1
Units: millimeters
Description: <p>mean zone of inhibition against O1-1</p>
http://lod.bco-dmo.org/id/dataset-parameter/545961.rdf
Name: O2_8
Units: millimeters
Description: <p>mean zone of inhibition against O2-8</p>
http://lod.bco-dmo.org/id/dataset-parameter/545962.rdf
Name: O2_12
Units: millimeters
Description: <p>mean zone of inhibition against O2-12</p>
http://lod.bco-dmo.org/id/dataset-parameter/545963.rdf
Name: R1_13
Units: millimeters
Description: <p>mean zone of inhibition against R1-13</p>
http://lod.bco-dmo.org/id/dataset-parameter/545964.rdf
Name: R5_5
Units: millimeters
Description: <p>mean zone of inhibition against R5-5</p>
http://lod.bco-dmo.org/id/dataset-parameter/545965.rdf
Name: R5_13
Units: millimeters
Description: <p>mean zone of inhibition against R5-13</p>
http://lod.bco-dmo.org/id/dataset-parameter/545966.rdf
Name: R5_29
Units: millimeters
Description: <p>mean zone of inhibition against R5-29</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
1591
https://datadocs.bco-dmo.org/file/l880yJkh1ornzq/assays.csv
assays.csv
Primary data file for dataset ID 544898
download
1591
https://datadocs.bco-dmo.org/file/4W35pNVclvgEYG/assays_Aeby_2014.csv
assays_Aeby_2014.csv
Antibacterial assays of Montipora capitata extracts
D. Gochfeld (UMiss)
version: 2014-08-28
download
https://osprey.bco-dmo.org/dataset/544898/data/download
download
onLine
dataset
<p><strong>Mucus sampling:</strong>&nbsp; Mucus was obtained from the surfaces of replicate orange and red colonies of Montipora capitata collected in Kaneohe Bay, Oahu, using a sterile pipettor and frozen immediately.</p>
<p><strong>Bacterial strains tested:</strong> &nbsp;The strains used for the antibacterial assays were selected as model systems to represent a range of potential bacterial pathogens from the marine environment, including known coral pathogens (Aurantimonas coralicida, Serratia marcescens, Vibrio coraliilyticus, Vibrio shiloi) and human enteric bacteria that have the potential to enter near-shore waters and can survive in the marine environment (Yersinia enterocolitica), and strains previously isolated from the surfaces of Hawaiian corals (Klebsiella pneumonia, Vibrio agarivorans) (Gochfeld &amp; Aeby 2008). &nbsp;In addition, bacterial strains isolated from surfaces of Montipora capitata (OCN001, OCN002, OCN003, OCN008, O1-1, O2-8, R1-13, R5-5, R5-29) by Dr. Sean Callahan’s lab at University of Hawaii were also used. &nbsp;Two of these, OCN002 and OCN008, are pathogens associated with Montipora White Syndrome (Ushijima et al. 2012, 2014).</p>
<p><strong>Growth inhibition assay</strong> (Gochfeld &amp; Aeby 2008):&nbsp; Assays were run in triplicate in 96 well plates. Plates contained 3 wells of bacteria + each mucus sample (n = 5 replicate corals of 2 color morphs), along with the following controls:&nbsp; bacteria only, media only, and mucus solution to control for the coloration of extracts.&nbsp; Twenty-four hour bacterial cultures in exponential growth were diluted to optical density (OD) 600, and 100 μl were added to each well. 10 μl of mucus were added to the experimental wells. Wells were mixed by carefully pipetting their contents, and an initial reading (time 0) of OD was made on a BioTek Synergy™ HT Multi-Detection Microplate Reader. Plates were then covered with foil and incubated on a shaker for 24 h and a final reading was made. Prior to each reading, wells were mixed again to resuspend any settled material.&nbsp;</p>
Specified by the Principal Investigator(s)
<p>To compare the growth curves from assays performed on different plates and at different times, we controlled for natural bacterial growth in each assay by subtracting the mean optical density of the 3 control wells (media + bacteria) from the optical density of each well containing a mucus sample in that assay.&nbsp; In addition, the mean optical density of the 2 wells containing each mucus solution was also subtracted from those wells to control for any color of the mucus.</p>
<p><strong>BCO-DMO Processing:</strong><br />
added conventional header with dataset name, PI name, version date, reference information<br />
renamed parameters to BCO-DMO standard<br />
replaced blanks and / with underscores</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
plate reader
plate reader
PI Supplied Instrument Name: plate reader PI Supplied Instrument Description:BioTek Synergy™ HT Multi-Detection Microplate Reader Instrument Name: plate reader Instrument Short Name: Instrument Description: Plate readers (also known as microplate readers) are laboratory instruments designed to detect biological, chemical or physical events of samples in microtiter plates. They are widely used in research, drug discovery, bioassay validation, quality control and manufacturing processes in the pharmaceutical and biotechnological industry and academic organizations. Sample reactions can be assayed in 6-1536 well format microtiter plates. The most common microplate format used in academic research laboratories or clinical diagnostic laboratories is 96-well (8 by 12 matrix) with a typical reaction volume between 100 and 200 uL per well. Higher density microplates (384- or 1536-well microplates) are typically used for screening applications, when throughput (number of samples per day processed) and assay cost per sample become critical parameters, with a typical assay volume between 5 and 50 µL per well. Common detection modes for microplate assays are absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, and fluorescence polarization. From: http://en.wikipedia.org/wiki/Plate_reader, 2014-09-0-23.
Deployment: Aeby_2014
Aeby_2014
Hawaii_reef
shoreside
Aeby_2014
Greta Aeby
University of Hawaiʻi at Mānoa
Hawaii_reef
shoreside