http://lod.bco-dmo.org/id/dataset/544904
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2015-01-15
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Chemical fingerprints of Montipora capitata aqueous extracts collected in Kaneohe Bay, Oahu, Hawaii during 2014
2014-08-28
publication
2014-08-28
revision
BCO-DMO Linked Data URI
2014-08-28
creation
http://lod.bco-dmo.org/id/dataset/544904
Deborah J. Gochfeld
University of Mississippi
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Gochfeld, D. J. (2014) Chemical fingerprints of Montipora capitata aqueous extracts collected in Kaneohe Bay, Oahu, Hawaii during 2014. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2014-08-28) Version Date 2014-08-28 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/544904 [access date]
Chemical fingerprints of Montipora capitata aqueous extracts Dataset Description: <p>Data access is restricted. Please contact the PI for further information.</p>
<p><strong>Related datasets:</strong></p>
<p><a href="http://www.bco-dmo.org/dataset/544870">Montipora antibacterial-aqueous</a><br />
<a href="http://www.bco-dmo.org/dataset/544898">Montipora antibacterial-mucus</a><br />
<a href="http://www.bco-dmo.org/dataset/544862">Montipora antibacterial-organic</a><br />
<a href="http://www.bco-dmo.org/dataset/545991">MWS accession numbers</a><br />
<a href="http://www.bco-dmo.org/dataset/544910">MWS lesion progression</a></p>
<p><strong>Related references:</strong></p>
<p>Deborah J. Gochfeld &amp; Haidy N. Kamel &amp; Julie B. Olson &amp; Robert W. Thacker. 2012. Trade-offs in defensive metabolite production but not ecological function in healthy and diseased sponges. J Chem Ecol 38:451-462. DOI 10.1007/s10886-012-0099-5.</p> Methods and Sampling: <p><strong>Preparation of extracts</strong>:&nbsp; Coral samples of orange and red colonies of Montipora capitata were collected in Kaneohe Bay, Oahu.&nbsp; Frozen samples were extracted in Millipore® water, which was replaced daily for 3 d. Extracts were filtered, lyophilized, and weighed.&nbsp; Extracts were resuspended in Millipore® water to a concentration of 10 mg/ml.</p>
<p><strong>Chemical fingerprinting:</strong>&nbsp; To generate chemical profiles, extracts were injected onto a Waters Alliance® 2695 High Performance Liquid Chromatography (HPLC) system (Phenomenex Syneri Hydro RP 4 um column; 250 x 4.6 mm; linear gradient from 100% water+0.1% acetic acid to 100% methanol+0.1% acetic acid) interfaced with a Waters 2998 Photodiode Array Detector.&nbsp; Spectra at 254 nm were generated.&nbsp; Each peak observed at the same retention time in each extract represents a particular compound, relative concentrations of which can be compared between extracts.&nbsp;&nbsp;</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0961384 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0961384
completed
Deborah J. Gochfeld
University of Mississippi
662-915-6769
National Center for Natural Products Research P.O. Box 1848
University
MS
38677
USA
gochfeld@olemiss.edu
pointOfContact
asNeeded
Dataset Version: 2014-08-28
Unknown
color_morph
coral
peak_1
peak_2
peak_3
peak_4
peak_5
peak_6
peak_7
peak_8
peak_9
peak_10
peak_11
peak_12
HPLC
theme
None, User defined
No BCO-DMO term
sample identification
featureType
BCO-DMO Standard Parameters
High-Performance Liquid Chromatograph
instrument
BCO-DMO Standard Instruments
Aeby_2014
service
Deployment Activity
Kaneohe Bay, Oahu, Hawaii
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Host-environment-pathogen interactions in a model coral disease system
https://osprey.bco-dmo.org/project/525619
Host-environment-pathogen interactions in a model coral disease system
<p><em>Extracted from the NSF award abstract:</em></p>
<p>Diseases of marine organisms have emerged as a serious problem contributing to the decline of coral reef resources worldwide. Loss of coral reef habitats carry social and economic implications especially in island states, such as Hawaii, which depend on reefs for food, shoreline protection and tourism. Our ability to manage coral diseases is hampered by a lack of knowledge of which environmental variables affect disease, mechanisms of host defense, and the etiology of most of the numerous described coral diseases. The PIs of this project discovered a coral disease system that can be used as a model to explore many components of the host-environment-pathogen triangle of disease causation. Montipora white syndrome (MWS) is an infectious disease that results in progressive tissue loss on colonies of Montipora capitata, and has been found on reefs throughout the Hawaiian archipelago. It is particularly prevalent in Kaneohe Bay, Oahu, which has a long history of reduced water quality, and this suboptimal environment sets the stage where host-pathogen interactions occur. In Kaneohe Bay, M. capitata is a major reef-building species, and is found in two color morphs (red and orange) that harbor different clades of zooxanthellae. During preliminary surveys, the PIs discovered intraspecific variability in response to MWS between color morphs. Although the red morph was dominant within survey transects (80% of the colonies), the orange morph was disproportionately affected by MWS (70% of the affected colonies). Microbial studies found a shift in bacterial communities on MWS-affected and healthy M. capitata and allowed identification of potential pathogens. Numerous bacterial strains were cultured and screened for pathogenicity and three strains, which produced lesions, were identified as potential pathogens. Two of the putative pathogens (Vibrio spp.) produced diffuse tissue whereas the other bacterial strain (Pseudoalteromonas sp.) produced acute tissue loss.</p>
<p>In the field, the PIs also observed two patterns of tissue loss on M. capitata; a slow, chronic pattern of tissue loss, which they followed through time with tagged colonies (chronic MWS), but also a rapid onset of acute tissue loss (acute MWS). Thus they discovered an infectious coral disease that results in significant coral mortality that has the unique component of differences in disease susceptibility among color morphs. The PIs identified three potential bacterial pathogens that will be used to investigate underlying factors affecting the coral-environment-pathogen triad of disease causation. The Hawaii Institute of Marine Biology (HIMB) is located within Kaneohe Bay allowing year-round access to reefs for research on Montipora white syndrome. The goal of this project is to investigate the host- environment-pathogen triangle of disease causation for Montipora white syndrome. The objectives of this research will be to: 1) investigate mechanisms contributing to differential disease resistance in red (less susceptible) vs. orange (more susceptible) morphs of M. capitata. The PIs will compare antimicrobial activity in the holobiont, mucus and mucus-associated bacteria of the two color morphs of M. capitata, and will compare the natural coral-associated microbial flora between the two color morphs; 2) use manipulative aquarium studies to determine whether environmental stressors (elevated temperature, nutrient stress) differentially affect the progression or transmission efficiency of MWS in red vs. orange morphs of M. capitata; 3) use challenge experiments to confirm the role of bacterial pathogens as causative agents of MWS, and to determine the response of red vs. orange morphs of M. capitata to three putative pathogens. This project will involve a multidisciplinary team to provide a broader perspective of coral disease processes. This will be the first comprehensive study conducted on a coral disease in Hawaii.</p>
<p><em>Related Publications:</em></p>
<p>Ushijima, B, Videau, P, Burger, A, Shore-Maggio, A, Runyon, C, Sudek, M, Aeby, G and S. Callahan. 2014. Vibrio coralliilyticus strain OCN008 is an etiological agent of acute Montipora white syndrome. Applied & Environ Microbiology doi:10.1128/AEM.03463-13.</p>
<p>Ushijima B, Videau P, Aeby GS, Callahan SM. 2013. Draft Genome Sequence of Vibrio coralliilyticus Strain OCN008, Isolated from Kane'ohe Bay, Hawai'i. Genome Announc. 2013 Oct 3;1(5). doi:pii: e00786-13. 10.1128/genomeA.00786-13. PMID: 24092784</p>
<p>Ushijima B, Smith A, Aeby GS, Callahan SM (2012) Vibrio owensii Induces the Tissue Loss Disease Montipora White Syndrome in the Hawaiian Reef Coral Montipora capitata. PLoS ONE 7: e46717.</p>
coral-pathogen interaction
largerWorkCitation
project
eng; USA
biota
oceans
Kaneohe Bay, Oahu, Hawaii
2014-08-28
Kaneohe Bay, Oahu, Hawaii (21 26' N, 157 47' W)
0
BCO-DMO catalogue of parameters from Chemical fingerprints of Montipora capitata aqueous extracts collected in Kaneohe Bay, Oahu, Hawaii during 2014
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/545902.rdf
Name: color_morph
Units: unitless
Description: <p>color morph of coral</p>
http://lod.bco-dmo.org/id/dataset-parameter/545903.rdf
Name: coral
Units: unitless
Description: <p>coral fragment identification</p>
http://lod.bco-dmo.org/id/dataset-parameter/545904.rdf
Name: peak_1
Units: unitless
Description: <p>Relative concentration of coral extract compound from peak 1 from the 254 nm spectra</p>
http://lod.bco-dmo.org/id/dataset-parameter/545905.rdf
Name: peak_2
Units: unitless
Description: <p>Relative concentration of coral extract compound from peak 2 from the 254 nm spectra</p>
http://lod.bco-dmo.org/id/dataset-parameter/545906.rdf
Name: peak_3
Units: unitless
Description: <p>Relative concentration of coral extract compound from peak 3 from the 254 nm spectra</p>
http://lod.bco-dmo.org/id/dataset-parameter/545907.rdf
Name: peak_4
Units: unitless
Description: <p>Relative concentration of coral extract compound from peak 4 from the 254 nm spectra</p>
http://lod.bco-dmo.org/id/dataset-parameter/545908.rdf
Name: peak_5
Units: unitless
Description: <p>Relative concentration of coral extract compound from peak 5 from the 254 nm spectra</p>
http://lod.bco-dmo.org/id/dataset-parameter/545909.rdf
Name: peak_6
Units: unitless
Description: <p>Relative concentration of coral extract compound from peak 6 from the 254 nm spectra</p>
http://lod.bco-dmo.org/id/dataset-parameter/545910.rdf
Name: peak_7
Units: unitless
Description: <p>Relative concentration of coral extract compound from peak 7 from the 254 nm spectra</p>
http://lod.bco-dmo.org/id/dataset-parameter/545911.rdf
Name: peak_8
Units: unitless
Description: <p>Relative concentration of coral extract compound from peak 8 from the 254 nm spectra</p>
http://lod.bco-dmo.org/id/dataset-parameter/545912.rdf
Name: peak_9
Units: unitless
Description: <p>Relative concentration of coral extract compound from peak 9 from the 254 nm spectra</p>
http://lod.bco-dmo.org/id/dataset-parameter/545913.rdf
Name: peak_10
Units: unitless
Description: <p>Relative concentration of coral extract compound from peak 10 from the 254 nm spectra</p>
http://lod.bco-dmo.org/id/dataset-parameter/545914.rdf
Name: peak_11
Units: unitless
Description: <p>Relative concentration of coral extract compound from peak 11 from the 254 nm spectra</p>
http://lod.bco-dmo.org/id/dataset-parameter/545915.rdf
Name: peak_12
Units: unitless
Description: <p>Relative concentration of coral extract compound from peak 12 from the 254 nm spectra</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
1918
https://datadocs.bco-dmo.org/file/l880yJkhOxY7B5/fingerprints.csv
fingerprints.csv
Primary data file for dataset ID 544904
download
https://osprey.bco-dmo.org/dataset/544904/data/download
download
onLine
dataset
<p><strong>Preparation of extracts</strong>:&nbsp; Coral samples of orange and red colonies of Montipora capitata were collected in Kaneohe Bay, Oahu.&nbsp; Frozen samples were extracted in Millipore® water, which was replaced daily for 3 d. Extracts were filtered, lyophilized, and weighed.&nbsp; Extracts were resuspended in Millipore® water to a concentration of 10 mg/ml.</p>
<p><strong>Chemical fingerprinting:</strong>&nbsp; To generate chemical profiles, extracts were injected onto a Waters Alliance® 2695 High Performance Liquid Chromatography (HPLC) system (Phenomenex Syneri Hydro RP 4 um column; 250 x 4.6 mm; linear gradient from 100% water+0.1% acetic acid to 100% methanol+0.1% acetic acid) interfaced with a Waters 2998 Photodiode Array Detector.&nbsp; Spectra at 254 nm were generated.&nbsp; Each peak observed at the same retention time in each extract represents a particular compound, relative concentrations of which can be compared between extracts.&nbsp;&nbsp;</p>
Specified by the Principal Investigator(s)
<p>Peaks from the 254 nm spectra were integrated to generate areas under the curve (in absorbance units).&nbsp; These areas can then be compared among extracts.</p>
<p><strong>BCO-DMO Processing:</strong><br />
- added conventional header with dataset name, PI name, version date<br />
- renamed parameters to BCO-DMO standard or to match other datasets in project<br />
- replaced blanks with underscores<br />
- replaced color_morph code with color name: 1=orange; 2=red</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
HPLC
HPLC
PI Supplied Instrument Name: HPLC PI Supplied Instrument Description:Waters Alliance® 2695 High Performance Liquid Chromatography (HPLC) system (Phenomenex Syneri Hydro RP 4 um column; 250 x 4.6 mm; linear gradient from 100% water+0.1% acetic acid to 100% methanol+0.1% acetic acid) interfaced with a Waters 2998 Photodiode Array Detector. Instrument Name: High-Performance Liquid Chromatograph Instrument Short Name:HPLC Instrument Description: A High-performance liquid chromatograph (HPLC) is a type of liquid chromatography used to separate compounds that are dissolved in solution. HPLC instruments consist of a reservoir of the mobile phase, a pump, an injector, a separation column, and a detector. Compounds are separated by high pressure pumping of the sample mixture onto a column packed with microspheres coated with the stationary phase. The different components in the mixture pass through the column at different rates due to differences in their partitioning behavior between the mobile liquid phase and the stationary phase. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB11/
Deployment: Aeby_2014
Aeby_2014
Hawaii_reef
shoreside
Aeby_2014
Greta Aeby
University of Hawaiʻi at Mānoa
Hawaii_reef
shoreside