http://lod.bco-dmo.org/id/dataset/545991
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2015-01-20
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
GenBank accession numbers for pathogens identified as causing Montipora White Syndrome in corals sampled from Kaneohe bay, Oahu, Hawaii during 2014
2014-08-28
publication
2014-08-28
revision
BCO-DMO Linked Data URI
2014-08-28
creation
http://lod.bco-dmo.org/id/dataset/545991
Greta Aeby
University of Hawaiʻi at Mānoa
principalInvestigator
Sean Callahan
University of Hawai'i
principalInvestigator
Evelyn Cox
University of Hawai'i
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Aeby, G., Callahan, S., Cox, E. (2014) GenBank accession numbers for pathogens identified as causing Montipora White Syndrome in corals sampled from Kaneohe bay, Oahu, Hawaii during 2014. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2014-08-28) Version Date 2014-08-28 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/545991 [access date]
GenBank accession numbers for pathogens identified as causing MWS from Kaneohe bay, Oahu, Hawaii Dataset Description: <p><strong>Related datasets:</strong></p>
<p><a href="http://www.bco-dmo.org/dataset/544870">Montipora antibacterial-aqueous</a><br />
<a href="http://www.bco-dmo.org/dataset/544898">Montipora antibacterial-mucus</a><br />
<a href="http://www.bco-dmo.org/dataset/544862">Montipora antibacterial-organic</a><br />
<a href="http://www.bco-dmo.org/dataset/544904">Montipora chemical fingerprints</a><br />
<a href="http://www.bco-dmo.org/dataset/544910">MWS lesion progression</a></p>
<p><strong>Related references:</strong></p>
<p>Ushijima et al. 2012: <em>Vibrio owensii</em> Induces the Tissue Loss Disease <em>Montipora</em> White Syndrome in the Hawaiian Reef Coral <em>Montipora capitata. PLoS ONE</em> 7: e46717.</p>
<p>Ushijima et al. 2013: Draft Genome Sequence of <em>Vibrio coralliilyticus</em> Strain OCN008, Isolated from Kāne'ohe Bay, Hawai'i. <em>Genome Announcements</em>. 1:e00786-13-e00786-13.</p>
<p>Ushijima et al. 2014: <em>Vibrio coralliilyticus</em> Strain OCN008 Is an Etiological Agent of Acute <em>Montipora</em> White Syndrome. <em>Applied and Environmental Microbiology</em> 80:2102-2109.</p> Methods and Sampling: <p>See the publications above for detailed methodology.</p>
<p>For accession numbers JX127210 to JX127222 from Ushijima et al. 2012, <em>PLoS ONE</em><br />
For accession number AVOO00000000 from Ushijima et al. 2013, <em>Genome announcements</em>.<br />
For accession numbers KF042020 to KF042038 from Ushijima et al. 2014, <em>Applied and Environmental Microbiology</em>.</p>
<p><strong>Brief summary of methods:</strong></p>
<p>Samples were collected from Kaneohe Bay, Oahu, Hawaii in 2011, 2012, and 2013.</p>
<p>Bacteria were isolated using standard microbiology techniques. DNA was extracted using a phenol-chloroform technique. PCR amplification was performed using DNA oligos. Refer to publications mentioned above for detailed methodology, statistical analyses, and results.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0961814 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0961814
onGoing
Greta Aeby
University of Hawaiʻi at Mānoa
(808) 236-7437
Hawaii Institute of Marine Biology PO Box 1346
Kane‘ohe
HI
96744
USA
greta@hawaii.edu
pointOfContact
Sean Callahan
University of Hawai'i
808 956-8015
207 Snyder Hall 2538 McCarthy Mall
Honolulu
HI
96822
USA
scallaha@hawaii.edu
pointOfContact
Evelyn Cox
University of Hawai'i
91-1001 Farrington Highway
Kapolei
HI
96707
USA
fcox@hawaii.edu
pointOfContact
asNeeded
Dataset Version: 2014-08-28
Unknown
Genbank_accession_num
organism
description
accession_link
theme
None, User defined
accession number
taxon
No BCO-DMO term
featureType
BCO-DMO Standard Parameters
Aeby_2014
service
Deployment Activity
Kaneohe Bay, Oahu, Hawaii
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Host-environment-pathogen interactions in a model coral disease system
https://osprey.bco-dmo.org/project/525619
Host-environment-pathogen interactions in a model coral disease system
<p><em>Extracted from the NSF award abstract:</em></p>
<p>Diseases of marine organisms have emerged as a serious problem contributing to the decline of coral reef resources worldwide. Loss of coral reef habitats carry social and economic implications especially in island states, such as Hawaii, which depend on reefs for food, shoreline protection and tourism. Our ability to manage coral diseases is hampered by a lack of knowledge of which environmental variables affect disease, mechanisms of host defense, and the etiology of most of the numerous described coral diseases. The PIs of this project discovered a coral disease system that can be used as a model to explore many components of the host-environment-pathogen triangle of disease causation. Montipora white syndrome (MWS) is an infectious disease that results in progressive tissue loss on colonies of Montipora capitata, and has been found on reefs throughout the Hawaiian archipelago. It is particularly prevalent in Kaneohe Bay, Oahu, which has a long history of reduced water quality, and this suboptimal environment sets the stage where host-pathogen interactions occur. In Kaneohe Bay, M. capitata is a major reef-building species, and is found in two color morphs (red and orange) that harbor different clades of zooxanthellae. During preliminary surveys, the PIs discovered intraspecific variability in response to MWS between color morphs. Although the red morph was dominant within survey transects (80% of the colonies), the orange morph was disproportionately affected by MWS (70% of the affected colonies). Microbial studies found a shift in bacterial communities on MWS-affected and healthy M. capitata and allowed identification of potential pathogens. Numerous bacterial strains were cultured and screened for pathogenicity and three strains, which produced lesions, were identified as potential pathogens. Two of the putative pathogens (Vibrio spp.) produced diffuse tissue whereas the other bacterial strain (Pseudoalteromonas sp.) produced acute tissue loss.</p>
<p>In the field, the PIs also observed two patterns of tissue loss on M. capitata; a slow, chronic pattern of tissue loss, which they followed through time with tagged colonies (chronic MWS), but also a rapid onset of acute tissue loss (acute MWS). Thus they discovered an infectious coral disease that results in significant coral mortality that has the unique component of differences in disease susceptibility among color morphs. The PIs identified three potential bacterial pathogens that will be used to investigate underlying factors affecting the coral-environment-pathogen triad of disease causation. The Hawaii Institute of Marine Biology (HIMB) is located within Kaneohe Bay allowing year-round access to reefs for research on Montipora white syndrome. The goal of this project is to investigate the host- environment-pathogen triangle of disease causation for Montipora white syndrome. The objectives of this research will be to: 1) investigate mechanisms contributing to differential disease resistance in red (less susceptible) vs. orange (more susceptible) morphs of M. capitata. The PIs will compare antimicrobial activity in the holobiont, mucus and mucus-associated bacteria of the two color morphs of M. capitata, and will compare the natural coral-associated microbial flora between the two color morphs; 2) use manipulative aquarium studies to determine whether environmental stressors (elevated temperature, nutrient stress) differentially affect the progression or transmission efficiency of MWS in red vs. orange morphs of M. capitata; 3) use challenge experiments to confirm the role of bacterial pathogens as causative agents of MWS, and to determine the response of red vs. orange morphs of M. capitata to three putative pathogens. This project will involve a multidisciplinary team to provide a broader perspective of coral disease processes. This will be the first comprehensive study conducted on a coral disease in Hawaii.</p>
<p><em>Related Publications:</em></p>
<p>Ushijima, B, Videau, P, Burger, A, Shore-Maggio, A, Runyon, C, Sudek, M, Aeby, G and S. Callahan. 2014. Vibrio coralliilyticus strain OCN008 is an etiological agent of acute Montipora white syndrome. Applied & Environ Microbiology doi:10.1128/AEM.03463-13.</p>
<p>Ushijima B, Videau P, Aeby GS, Callahan SM. 2013. Draft Genome Sequence of Vibrio coralliilyticus Strain OCN008, Isolated from Kane'ohe Bay, Hawai'i. Genome Announc. 2013 Oct 3;1(5). doi:pii: e00786-13. 10.1128/genomeA.00786-13. PMID: 24092784</p>
<p>Ushijima B, Smith A, Aeby GS, Callahan SM (2012) Vibrio owensii Induces the Tissue Loss Disease Montipora White Syndrome in the Hawaiian Reef Coral Montipora capitata. PLoS ONE 7: e46717.</p>
coral-pathogen interaction
largerWorkCitation
project
eng; USA
biota
oceans
Kaneohe Bay, Oahu, Hawaii
2014-08-28
Kaneohe Bay, Oahu, Hawaii (21 26' N, 157 47' W)
0
BCO-DMO catalogue of parameters from GenBank accession numbers for pathogens identified as causing Montipora White Syndrome in corals sampled from Kaneohe bay, Oahu, Hawaii during 2014
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/545999.rdf
Name: Genbank_accession_num
Units: unitless
Description: <p>GenBank accession number</p>
http://lod.bco-dmo.org/id/dataset-parameter/546000.rdf
Name: organism
Units: unitless
Description: <p>organism taxonomic name and strain</p>
http://lod.bco-dmo.org/id/dataset-parameter/546001.rdf
Name: description
Units: unitless
Description: <p>description of sequenced genetic material</p>
http://lod.bco-dmo.org/id/dataset-parameter/546002.rdf
Name: accession_link
Units: unitless
Description: <p>link to GenBank accession number page</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
5378
https://datadocs.bco-dmo.org/file/6YYMwnMhZAQzW9/accession_nums.csv
accession_nums.csv
Primary data file for dataset ID 545991
download
https://osprey.bco-dmo.org/dataset/545991/data/download
download
onLine
dataset
<p>See the publications above for detailed methodology.</p>
<p>For accession numbers JX127210 to JX127222 from Ushijima et al. 2012, <em>PLoS ONE</em><br />
For accession number AVOO00000000 from Ushijima et al. 2013, <em>Genome announcements</em>.<br />
For accession numbers KF042020 to KF042038 from Ushijima et al. 2014, <em>Applied and Environmental Microbiology</em>.</p>
<p><strong>Brief summary of methods:</strong></p>
<p>Samples were collected from Kaneohe Bay, Oahu, Hawaii in 2011, 2012, and 2013.</p>
<p>Bacteria were isolated using standard microbiology techniques. DNA was extracted using a phenol-chloroform technique. PCR amplification was performed using DNA oligos. Refer to publications mentioned above for detailed methodology, statistical analyses, and results.</p>
Specified by the Principal Investigator(s)
<p>DNA sequences were trimmed using Gene Construction Kit software.</p>
<p><strong>BCO-DMO Processing:</strong><br />
- added conventional header with dataset name, PI name, version date<br />
- renamed parameters to BCO-DMO standard or to match other datasets in project<br />
- replaced blanks with underscores</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Deployment: Aeby_2014
Aeby_2014
Hawaii_reef
shoreside
Aeby_2014
Greta Aeby
University of Hawaiʻi at Mānoa
Hawaii_reef
shoreside