<div><p>Collections were made in the vicinity of Long Key, FL, oceanside (N24o 49.791’ W80o 45.743’) and experimentation at the Keys Marine Laboratory, Long Key, FL.</p>
<p>Symbiont density within coral tissues was determined three times over the course of the experiment; (1) before exposure to elevated temperature, (2) when the heat treatment was terminated and (3) 5 weeks into the recovery period. Coral tissue was scraped from the colony surface and placed in 1.0 ml of 5% formalin. The length and width of the scar were measured and these dimensions (length x width) were used to estimate the surface of tissue removed. Subsequently, each tissue sample was homogenized and 9 µL aliquots were counted using a hemacytometer. A total of four replicate counts were conducted per tissue sample and mean symbiont density per mm^2 was calculated.</p></div>
pre- and post-bleach symbiont density in Porites divaricata
<div><p>Cell counts of symbionts by treatment of pre-bleached, bleached and recovering corals (<em>Porites divaricata</em>).</p>
<p><strong>Related Reference:</strong></p>
<p>Coffroth MA, Poland DM, Petrou EL, Brazeau DA, Holmberg JC (2010) Environmental Symbiont Acquisition May Not Be the Solution to Warming Seas for Reef-Building Corals. PLoS ONE 5(10): e13258. doi:10.1371/journal.pone.0013258.<a href="http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0013258" target="_blank"> http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0013258</a></p></div>
Symbiont density
<div><p>Cell counts between times (within a treatment) were compared using (repeated measures ANOVA using Greenhouse-Geisser correction for violation of assumption of sphericity, F(1.088,88.144)=137.119, p<0.001; within-subject contrasts, F(1,81)=153.654, p<0.001).</p>
<p><strong>BCO-DMO Processing:</strong></p>
<p>- original file: Coffroth et al 2010_ Data Summary_cell counts.xlsx<br />
- added conventional header with dataset name, PI name, version date, reference information<br />
- changed parameter names to be BCO-DMO compatible<br />
- replaced spaces with underscores - replaced Pur pflex -> Plexaura_flexuosa; BLcntrl -> bleached_control; UBLcntrl -> unbleached control<br />
- combined data from all 3 dates and reformatted to flat file.</p></div>
546131
Symbiont density
2015-01-21T14:34:32-05:00
2015-01-21T14:34:32-05:00
2023-07-07T16:10:26-04:00
urn:bcodmo:dataset:546131
Laboratory results on pre and post bleach symbiont density in Porites divaricata collected from the Florida Keys, Bahamas, Panama, and Mexico during 2010 (SymBioSys project)
false
Coffroth, M. A. (2015) Laboratory results on pre and post bleach symbiont density in Porites divaricata collected from the Florida Keys, Bahamas, Panama, and Mexico during 2010 (SymBioSys project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2015-01-29) Version Date 2015-01-29 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/546131 [access date]
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2015-01-29
false
2015-01-29
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