|Coffroth, Mary Alice||State University of New York at Buffalo (SUNY Buffalo)||Principal Investigator|
|Copley, Nancy||Woods Hole Oceanographic Institution (WHOI BCO-DMO)||BCO-DMO Data Manager|
Cell counts of symbionts by treatment of pre-bleached, bleached and recovering corals (Porites divaricata).
Coffroth MA, Poland DM, Petrou EL, Brazeau DA, Holmberg JC (2010) Environmental Symbiont Acquisition May Not Be the Solution to Warming Seas for Reef-Building Corals. PLoS ONE 5(10): e13258. doi:10.1371/journal.pone.0013258. http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0013258
Collections were made in the vicinity of Long Key, FL, oceanside (N24o 49.791’ W80o 45.743’) and experimentation at the Keys Marine Laboratory, Long Key, FL.
Symbiont density within coral tissues was determined three times over the course of the experiment; (1) before exposure to elevated temperature, (2) when the heat treatment was terminated and (3) 5 weeks into the recovery period. Coral tissue was scraped from the colony surface and placed in 1.0 ml of 5% formalin. The length and width of the scar were measured and these dimensions (length x width) were used to estimate the surface of tissue removed. Subsequently, each tissue sample was homogenized and 9 µL aliquots were counted using a hemacytometer. A total of four replicate counts were conducted per tissue sample and mean symbiont density per mm^2 was calculated.
Cell counts between times (within a treatment) were compared using (repeated measures ANOVA using Greenhouse-Geisser correction for violation of assumption of sphericity, F(1.088,88.144)=137.119, p<0.001; within-subject contrasts, F(1,81)=153.654, p<0.001).
- original file: Coffroth et al 2010_ Data Summary_cell counts.xlsx
- added conventional header with dataset name, PI name, version date, reference information
- changed parameter names to be BCO-DMO compatible
- replaced spaces with underscores - replaced Pur pflex -> Plexaura_flexuosa; BLcntrl -> bleached_control; UBLcntrl -> unbleached control
- combined data from all 3 dates and reformatted to flat file.
|lab||laboratory identification; for mapping puposes||unitless|
|lat||latitude of lab; north is positive||decimal degrees|
|lon||londitude of lab; east is positive||decimal degrees|
|description||timing of sample collection: pre- or post-bleaching||unitless|
|date||date of sample collection||yyyy-mm-dd|
|culture_inoculant||Cultures 311, 702, A001 and Plexaura flexuosa (Pur Pflex) were obtained from the BURR Culture Collection and used to inoculate bleached colonies of Porites divaricata (Pd).||unitless|
|symbiont_type||The symbiont type of the culture used to inoculate bleached colonies of Porites divaricata. Symbiont type based on sequence variation in the chloroplast 23S rDNA (cp-type). Notations based on symbiont clade (Letter) and fragment size (number in bp). For example, A188 is a symbiont within clade A where the 23S rDNA fragment is 188bp in length.||unitless|
|count_1||symbiont cells in first subsample||cells|
|count_2||symbiont cells in second subsample||cells|
|count3||symbiont cells in third subsample||cells|
|count_4||symbiont cells in fourth subsample||cells|
|mean_count||average number of cells in sub samples||cells|
|mean_x10000||average number of cells in sub samples x 10^4||cells x 10^4|
|length||length of scar from tissue removal||millimeters|
|width||width of scar from tissue removal||millimeters|
|type||symbiont type: H=?; S=?; LIGHT=?; scar_toss=discarded??||unitless|
|surf_area||surface area of coral tissue removed||mm^2|
|cells_mm2||symbiont cell density||cells/mm2|
|cells_x10000_mm2||symbiont cell density x 10^4||cells x 10^4/mm2|
|std_dev||standard deviation of cell density||cells x 10^4/mm2|
|Dataset-specific Instrument Name|| |
|Generic Instrument Name|| |
|Generic Instrument Description|| |
A hemocytometer is a small glass chamber, resembling a thick microscope slide, used for determining the number of cells per unit volume of a suspension. Originally used for performing blood cell counts, a hemocytometer can be used to count a variety of cell types in the laboratory. Also spelled as "haemocytometer". Description from: http://hlsweb.dmu.ac.uk/ahs/elearning/RITA/Haem1/Haem1.html.
|Start Date|| |
|End Date|| |
laboratory-based research on coral symbionts
The symbiosis between corals (Cnidaria:Hexacorallia:Scleractinia) and photosynthetic dinoflagellate symbionts (Alveolata: Dinophycea: Symbiodinium) provides the foundation and structure of the coral reef ecosystem, as well as significant contributions to global carbon and biogeochemical cycles. Given the importance of this symbiosis to the coral-algal holobiont and the reef ecosystem, understanding the mechanisms governing the establishment and long term maintenance of this symbiosis is essential. The overall aim of this project is to identify the mechanisms and selective processes that lead to the final assemblage of symbionts harbored by adult hosts. This question will be approached from two perspectives, ecologic and genomic, with the specific aims of determining (1) if different Symbiodinium strains differentially affect fitness of corals as the adult settles into a mature symbiosis (2) if competition among symbionts or environmental conditions contribute to the final host-symbiont pairing and (3) how host/symbiont transcriptomes varying as the symbiont community within a host is winnowed to the final assemblage found in the adult host. Traits that directly affect coral fitness (i.e. growth, survivorship, energy production) will be measured under different environmental conditions over the ontogeny of coral recruits that are experimentally infected with different types of Symbiodinium. Concurrently, high throughput gene expression profiling will be used to follow changes in gene expression between host and symbiont. Together, these data will be used to validate or falsify the hypotheses that the final symbiont assemblage found in the adult host is determined by (a) host selection (b) competition among symbionts and/or (c) environmental condition.
This study pools the expertise of two labs that have focused on these aspects of the symbiosis. The Coffroth lab pioneered the studies on early ontogeny of the symbiosis and symbiont diversity and will continue to take the lead in the ecological studies. The Medina lab is at the forefront in the development and utilization of genomic technology to study transcriptomic changes during the establishment and breakdown of the symbiosis. Furthermore, the Medina lab has the coral microarrays to be used in this study and in 2009 will also have oligo arrays for two Symbiodinium species based on 454 EST data. Although several groups have initial studies of the host transcriptome, none have combined an approach that examines the host and the symbiont in a single experiment. This will be a powerful approach as it will allow the investigators to track complementary changes in gene expression between host and symbiont and relate those to turnover in the symbiont community as the final symbiont complement is established.
The data resulting form the study will bridge an important gap in our understanding of the establishment and maintenance of coral-Symbiodinium symbiosis. Understanding the mechanism(s) regulating the establishment of the symbiosis will broaden our knowledge and help to predict the response of this symbiosis to future climate conditions. As in the past, the genomic tools (arrays, ESTs) will be made readily available to researchers via array distribution at cost, microarray analysis training, or sequence data, providing valuable resources to continue exploring these systems.
In conjunction the Aquarium of Niagara, Coffroth will develop educational and outreach programs to train and disseminate information on coral reefs to local area teachers and the general public. The Medina lab will continue to produce science and environment podcasts in multiple languages (English, Spanish and Hmong) with undergraduate students at UC Merced and will continue to collaborate with the California Academy of Sciences (CAS) in their coral reef outreach efforts. Additionally, this work will result in the training and mentoring of a postdoctoral fellow, at least one graduate student and at least 2 undergraduates. Through this project these students will have the opportunity to participate in research in both a lab and field setting, learning a range of ecological, molecular and algal culturing techniques. The extensive culture collection housed at the University at Buffalo is an important resource that is available to researchers worldwide which the proposed funding will help to maintain. Our EST annotations are publicly available through our EST database (http://montastraea.psu.edu/SymBioSys/).
|NSF Division of Ocean Sciences (NSF OCE)|
|NSF Division of Ocean Sciences (NSF OCE)|