http://lod.bco-dmo.org/id/dataset/546223
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2015-01-23
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Experimental results: coral calcification, chlorophyll-a content, algal density, energy reserves, and tissue biomass from samples of reef systems collected from northwest Fiji in 2011
2015-09-23
publication
2015-09-23
revision
BCO-DMO Linked Data URI
2015-09-23
creation
http://lod.bco-dmo.org/id/dataset/546223
Andréa G. Grottoli
Ohio State University
principalInvestigator
Wei-Jun Cai
University of Delaware
principalInvestigator
Mark E. Warner
University of Delaware
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Grottoli, A. G., Warner, M. E., Cai, W. (2015) Experimental results: coral calcification, chlorophyll-a content, algal density, energy reserves, and tissue biomass from samples of reef systems collected from northwest Fiji in 2011. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 23 Sept 2015) Version Date 2015-09-23 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/546223 [access date]
Coral calcification, chlorophyll a content, algal density, energy reserves, tissue biomass. Dataset Description: <p>Coral calcification, chlorophyll <em>a</em> content, algal density, energy reserves, and tissue biomass from experiments carried out on coral samples from the taxa: <em>Acropora millepora, Pocillopora damicornis, Montipora monasteriata</em>, and T<em>urbinaria reniformis</em>.</p> Methods and Sampling: <p>Full details of the experimental design are in:<br />
Schoepf V<strong>, </strong>Grottoli AG, Warner ME, Cai W-J, Melman TF, Pettay DT, Hoadley K, Matsui Y, Baumann JH, Wang Y, Xu H, Li Q, &amp; Hu X. 2013. Coral energy reserves and calcification in a high-CO<sub>2</sub> world at two temperatures. PLoS ONE<em>, </em>8(10): e75049. doi:<a href="http://dx.doi.org/10.1371/journal.pone.0075049" target="_blank">10.1371/journal.pone.0075049</a>.</p>
<p>A brief description of the analytical methods follows.</p>
<p>Coral collection (April 2011): northwest Fiji (17°29'19"S, 177°23'39"E)<br />
Experiment (July/August 2011): Reef Systems Coral Farm, New Albany, Ohio, USA (40°07'24"N, 82°46'55"W)</p>
<p><strong>Calcification. </strong>Net calcification was determined using the buoyant weight technique (Jokiel et al. 1978). Each coral fragment was buoyantly weighed at the beginning, middle (after 11 experimental days), and at the end of the experiment (after 23 experimental days). As such, it was possible to assess if calcification rates varied during the experiment. Daily calcification rates were calculated as the difference between initial, middle, and final weights, divided by the respective number of days elapsed, and standardized to surface area (see below).</p>
<p>For tissue analyses, corals were frozen at −80 degrees C and a total of three branch tips or growing edge pieces were saved from each fragment for lipid, protein/carbohydrate, and tissue biomass analyses, respectively. The remaining tissue was airbrushed for chlorophyll <em>a</em> and endosymbiont density measurements.</p>
<p><strong>Chlorophyll <em>a</em> and endosymbiont density. </strong>Coral tissue was stripped off the coral skeleton with a waterpik containing 40 ml of synthetic seawater (Instant Ocean). The endosymbionts were isolated from the host tissue via centrifugation and then resuspended in 10 ml of synthetic seawater. For chlorophyll <em>a</em> concentrations, 1 ml of this algal suspension was pelleted and the cells lysed in 1 ml of 4 degree C methanol using a bead-beater for 60 seconds. Samples were then immediately placed on ice and allowed to extract for one hour in the dark. Samples were centrifuged to remove cellular debris and measured spectrophotometrically (λ = 652, 665, &amp; 750) on a 96-well plate reader. The equations for chlorophyll <em>a</em> in methanol described by Porra et al. 1989, along with path length correction (Warren 2008), were used to calculate chlorophyll <em>a</em> concentrations (pg/cell), and were then standardized to surface area (see below). Another 1 ml subsample of the algal suspension was preserved with 10 ul of 1% glutaraldehyde solution for endosymbiont quantification, which was calculated using 6 independent replicate counts on a hemocytometer, using a Nikon microphot-FXA epifluorescent microscope at 100× magnification. Photographs were analyzed through Image J using the analyze particles function.</p>
<p><strong>Energy reserves and tissue biomass</strong>. For all energy reserve and tissue biomass measurements, only branch tips or samples with a growing edge were used. While tissue composition may vary across the surface of a coral (Oku et al. 2002), this approach was used to allow for comparison with previously published studies (Grottoli et al. 2004, Rodrigues and Grottoli 2007, Levas et al. 2013). Soluble lipids (referred to hereafter simply as lipids) were extracted from a whole, ground coral sample (skeleton + animal tissue + algal endosymbiont) in a 2:1 chloroform:methanol solution for 1 hour (Grottoli et al. 2004, Rodrigues and Grottoli 2007), washed in 0.88% KCl followed by 100% chloroform and another wash with 0.88% KCl. The extract was dried to constant weight under a stream of pure nitrogen (UPH grade 5.0) and standardized to the ash-free dry weight.</p>
<p>Animal soluble protein and carbohydrate (referred to hereafter simply as protein and carbohydrate, respectively) were extracted from grinding a whole second branch tip of the same fragment (Rodrigues and Grottoli 2007). Briefly, Milli-Q water was added to the ground coral sample and the resulting slurry was sonicated (5 min) and then centrifuged twice (5000 rpm, 10 min) to separate the animal tissue from the skeleton and endosymbiotic algae. Protein and carbohydrate was extracted from the animal tissue only. One subsample of this animal tissue slurry was used for protein extraction using the bicinchoninic acid method (Smith et al. 1985) with bovine serum albumin as a standard (Pierce BCA Protein Assay Kit). A second subsample was used for carbohydrate quantification using the phenol-sulfuric acid method (Dubois et al. 1956) with glucose as a standard. Soluble animal protein and carbohydrate concentrations were standardized to the ash-free dry weight.</p>
<p>Tissue biomass was measured by drying a third branch tip of whole coral sample (skeleton + animal tissue + algal endosymbiont) to constant dry weight (24 hours, 60 degrees C) and burning it (6 hours, 450 degrees C). The difference between dry and burned weight was the ash free dry weight which was standardized to the surface area of this branch tip.</p>
<p><strong>Surface area. </strong>Surface area of plating <em>M. monasteriata</em> and <em>T. reniformis</em> fragments was determined using the aluminum foil technique (Marsh 1970), whereas surface area of branching <em>A. millepora</em> and <em>P. damicornis</em> fragments was determined using the single wax dipping technique (Stimson and Kinzie 1990, Veal et al. 2010) after the tissue had been removed. Natural wooden blocks of varying sizes and shapes were used as calibration standards (Veal et al. 2010). Wax dipping was conducted using household paraffin wax (Gulf Wax, Royal Oak Enterprises) heated to 65 degrees C. Dried coral skeletons and wooden calibration standards were maintained at room temperature prior to weighing.</p>
Funding provided by NSF Emerging Frontiers Division (NSF EF) Award Number: EF-1041124 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1041124
Funding provided by NSF Emerging Frontiers Division (NSF EF) Award Number: EF-1040940 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1040940
Funding provided by NSF Emerging Frontiers Division (NSF EF) Award Number: EF-1041070 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1041070
completed
Andréa G. Grottoli
Ohio State University
614-292-5782
School of Earth Sciences 125 South Oval Mall
Columbus
OH
43210
United States
grottoli.1@osu.edu
pointOfContact
Wei-Jun Cai
University of Delaware
302-831-2839
School of Marine Science and Policy Office: 014 Lammot DuPont Laboratory
Newark
DE
19716
USA
wcai@udel.edu
pointOfContact
Mark E. Warner
University of Delaware
302-645-4365
School of Marine Science and Policy 700 Pilottown Rd.
Lewes
DE
19958
USA
mwarner@udel.edu
pointOfContact
asNeeded
Dataset Version: 23 Sept 2015
Unknown
species
coral_ID
temp
pCO2
genotype
calcification_1st_half
calcification_2nd_half
chlorophyll_a
cell_density
lipid
protein
carbs
tissue_biomass
Nikon microphot-FXA epifluorescent microscope
Hemocytometer
theme
None, User defined
species scientific name (binomial)
sample identification
water temperature at measurement depth
Partial pressure of CO2
No BCO-DMO term
featureType
BCO-DMO Standard Parameters
Fluorescence Microscope
Hemocytometer
instrument
BCO-DMO Standard Instruments
Fiji_2011_Grottoli
RSCF_Grottoli_2011
service
Deployment Activity
northwest Fiji
New Albany, Ohio, USA
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA)
https://www.nsf.gov/funding/pgm_summ.jsp?pims_id=503477
Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA)
NSF Climate Research Investment (CRI) activities that were initiated in 2010 are now included under Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES). SEES is a portfolio of activities that highlights NSF's unique role in helping society address the challenge(s) of achieving sustainability. Detailed information about the SEES program is available from NSF (https://www.nsf.gov/funding/pgm_summ.jsp?pims_id=504707).
In recognition of the need for basic research concerning the nature, extent and impact of ocean acidification on oceanic environments in the past, present and future, the goal of the SEES: OA program is to understand (a) the chemistry and physical chemistry of ocean acidification; (b) how ocean acidification interacts with processes at the organismal level; and (c) how the earth system history informs our understanding of the effects of ocean acidification on the present day and future ocean.
Solicitations issued under this program:
NSF 10-530, FY 2010-FY2011
NSF 12-500, FY 2012
NSF 12-600, FY 2013
NSF 13-586, FY 2014
NSF 13-586 was the final solicitation that will be released for this program.
PI Meetings:
1st U.S. Ocean Acidification PI Meeting(March 22-24, 2011, Woods Hole, MA)
2nd U.S. Ocean Acidification PI Meeting(Sept. 18-20, 2013, Washington, DC)
3rd U.S. Ocean Acidification PI Meeting (June 9-11, 2015, Woods Hole, MA – Tentative)
NSF media releases for the Ocean Acidification Program:
Press Release 10-186 NSF Awards Grants to Study Effects of Ocean Acidification
Discovery Blue Mussels "Hang On" Along Rocky Shores: For How Long?
Discovery nsf.gov - National Science Foundation (NSF) Discoveries - Trouble in Paradise: Ocean Acidification This Way Comes - US National Science Foundation (NSF)
Press Release 12-179 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: Finding New Answers Through National Science Foundation Research Grants - US National Science Foundation (NSF)
Press Release 13-102 World Oceans Month Brings Mixed News for Oysters
Press Release 13-108 nsf.gov - National Science Foundation (NSF) News - Natural Underwater Springs Show How Coral Reefs Respond to Ocean Acidification - US National Science Foundation (NSF)
Press Release 13-148 Ocean acidification: Making new discoveries through National Science Foundation research grants
Press Release 13-148 - Video nsf.gov - News - Video - NSF Ocean Sciences Division Director David Conover answers questions about ocean acidification. - US National Science Foundation (NSF)
Press Release 14-010 nsf.gov - National Science Foundation (NSF) News - Palau's coral reefs surprisingly resistant to ocean acidification - US National Science Foundation (NSF)
Press Release 14-116 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: NSF awards $11.4 million in new grants to study effects on marine ecosystems - US National Science Foundation (NSF)
SEES-OA
largerWorkCitation
program
Interactive Effects of Temperature, Nutrients, and Ocean Acidification on Coral Physiology and Calcification
https://osprey.bco-dmo.org/project/528004
Interactive Effects of Temperature, Nutrients, and Ocean Acidification on Coral Physiology and Calcification
<p><em>Extracted from the NSF award abstract:</em> </p>
<p>Atmospheric and sea surface CO2 concentrations are expected to continue to increase substantially over the coming decades resulting in warmer and more acidic oceans, which will greatly stress the health of coral reefs. In addition, ocean margins where most corals live will also see continued increases in human-produced nutrient inputs. While there has recently been a considerable focus on how ocean acidification (due to higher CO2 alone) could negatively impact the growth of reef-building corals due to the projected loss in calcification, the combined impacts of CO2, temperature, and nutrients on coral physiology and calcification are poorly understood. This project will investigate the possible synergistic and antagonistic effects of elevated temperature, CO2, and nutrients on the physiology and internal calcifying chemistry of several species of corals in a laboratory setting. Research tools will include the assessment of coral energy reserves and metabolic demand, symbiotic algal physiology and molecular diversity, coral calcification, and direct measurement of the internal coral pH and carbonate concentration via microprobes. The results from this project have the potential to supply broad scientific impacts regarding how (or if) reef-building corals will survive future climate change scenarios, and will help establish several parameter ranges that could be used to strengthen ocean acidification and coral reef growth models.</p>
OA_coral_physiology
largerWorkCitation
project
eng; USA
oceans
northwest Fiji; New Albany, Ohio, USA
2015-09-23
Reef Systems Coral Farm, New Albany, Ohio, USA
0
BCO-DMO catalogue of parameters from Experimental results: coral calcification, chlorophyll-a content, algal density, energy reserves, and tissue biomass from samples of reef systems collected from northwest Fiji in 2011
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/546234.rdf
Name: species
Units: unitless
Description: <p>Taxonomic name of the coral species.</p>
http://lod.bco-dmo.org/id/dataset-parameter/546235.rdf
Name: coral_ID
Units: unitless
Description: <p>ID number of the coral sample.</p>
http://lod.bco-dmo.org/id/dataset-parameter/546236.rdf
Name: temp
Units: degrees Celsius ( C)
Description: <p>Water temperature.</p>
http://lod.bco-dmo.org/id/dataset-parameter/546237.rdf
Name: pCO2
Units: microatmospheres (uatm)
Description: <p>Partial pressure of CO2.</p>
http://lod.bco-dmo.org/id/dataset-parameter/546238.rdf
Name: genotype
Units: unitless
Description: <p>Genotype (parent colony) identifier.</p>
http://lod.bco-dmo.org/id/dataset-parameter/546239.rdf
Name: calcification_1st_half
Units: milligrams per day per square centimeter (mg/day/cm2)
Description: <p>Calcification 1st half: This is the daily calcification rate measured on the 11th day of the experiment using the buoyant weight method and standardized to the surface area of the coral fragment.</p>
http://lod.bco-dmo.org/id/dataset-parameter/546240.rdf
Name: calcification_2nd_half
Units: milligrams per day per square centimeter (mg/day/cm2)
Description: <p>Calcification 2nd half: This is the daily calcification rate measured on the 23rd day of the experiment using the buoyant weight method and standardized to the surface area of the coral fragment.</p>
http://lod.bco-dmo.org/id/dataset-parameter/546241.rdf
Name: chlorophyll_a
Units: micrograms per square centimeter (ug/cm2)
Description: <p>The chlorophyll a content of the endosymbiont fraction standardized to surface area of the fragment.</p>
http://lod.bco-dmo.org/id/dataset-parameter/546242.rdf
Name: cell_density
Units: x10^6 cells per square centimeter (cells/cm2)
Description: <p>The number of endosymbiont (Symbiodinium spp.) cells x10^6 per surface area of the coral fragment.</p>
http://lod.bco-dmo.org/id/dataset-parameter/546243.rdf
Name: lipid
Units: grams per grams dry weight (g/gdw)
Description: <p>The soluble lipid content of the combined animal host and endosymbiont fraction standardized to ash-free dry weight (i.e. gdw).</p>
http://lod.bco-dmo.org/id/dataset-parameter/546244.rdf
Name: protein
Units: grams per grams dry weight (g/gdw)
Description: <p>The soluble protein content of the animal host fraction standardized to ash-free dry weight (i.e. gdw).</p>
http://lod.bco-dmo.org/id/dataset-parameter/546245.rdf
Name: carbs
Units: grams per grams dry weight (g/gdw)
Description: <p>The soluble carbohydrate content of the animal host fraction standardized to ash-free dry weight (i.e. gdw).</p>
http://lod.bco-dmo.org/id/dataset-parameter/546246.rdf
Name: tissue_biomass
Units: milligrams per square centimeter (mg/cm2)
Description: <p>The ash-free dry weight of the whole tissue (animal host and endosymbiont) standardized to surface area.</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
14092
https://datadocs.bco-dmo.org/file/7DDMxo7fWvyYGD/coral_calc_and_energy.csv
coral_calc_and_energy.csv
Primary data file for dataset ID 546223
download
https://osprey.bco-dmo.org/dataset/546223/data/download
download
onLine
dataset
<p>Full details of the experimental design are in:<br />
Schoepf V<strong>, </strong>Grottoli AG, Warner ME, Cai W-J, Melman TF, Pettay DT, Hoadley K, Matsui Y, Baumann JH, Wang Y, Xu H, Li Q, &amp; Hu X. 2013. Coral energy reserves and calcification in a high-CO<sub>2</sub> world at two temperatures. PLoS ONE<em>, </em>8(10): e75049. doi:<a href="http://dx.doi.org/10.1371/journal.pone.0075049" target="_blank">10.1371/journal.pone.0075049</a>.</p>
<p>A brief description of the analytical methods follows.</p>
<p>Coral collection (April 2011): northwest Fiji (17°29'19"S, 177°23'39"E)<br />
Experiment (July/August 2011): Reef Systems Coral Farm, New Albany, Ohio, USA (40°07'24"N, 82°46'55"W)</p>
<p><strong>Calcification. </strong>Net calcification was determined using the buoyant weight technique (Jokiel et al. 1978). Each coral fragment was buoyantly weighed at the beginning, middle (after 11 experimental days), and at the end of the experiment (after 23 experimental days). As such, it was possible to assess if calcification rates varied during the experiment. Daily calcification rates were calculated as the difference between initial, middle, and final weights, divided by the respective number of days elapsed, and standardized to surface area (see below).</p>
<p>For tissue analyses, corals were frozen at −80 degrees C and a total of three branch tips or growing edge pieces were saved from each fragment for lipid, protein/carbohydrate, and tissue biomass analyses, respectively. The remaining tissue was airbrushed for chlorophyll <em>a</em> and endosymbiont density measurements.</p>
<p><strong>Chlorophyll <em>a</em> and endosymbiont density. </strong>Coral tissue was stripped off the coral skeleton with a waterpik containing 40 ml of synthetic seawater (Instant Ocean). The endosymbionts were isolated from the host tissue via centrifugation and then resuspended in 10 ml of synthetic seawater. For chlorophyll <em>a</em> concentrations, 1 ml of this algal suspension was pelleted and the cells lysed in 1 ml of 4 degree C methanol using a bead-beater for 60 seconds. Samples were then immediately placed on ice and allowed to extract for one hour in the dark. Samples were centrifuged to remove cellular debris and measured spectrophotometrically (λ = 652, 665, &amp; 750) on a 96-well plate reader. The equations for chlorophyll <em>a</em> in methanol described by Porra et al. 1989, along with path length correction (Warren 2008), were used to calculate chlorophyll <em>a</em> concentrations (pg/cell), and were then standardized to surface area (see below). Another 1 ml subsample of the algal suspension was preserved with 10 ul of 1% glutaraldehyde solution for endosymbiont quantification, which was calculated using 6 independent replicate counts on a hemocytometer, using a Nikon microphot-FXA epifluorescent microscope at 100× magnification. Photographs were analyzed through Image J using the analyze particles function.</p>
<p><strong>Energy reserves and tissue biomass</strong>. For all energy reserve and tissue biomass measurements, only branch tips or samples with a growing edge were used. While tissue composition may vary across the surface of a coral (Oku et al. 2002), this approach was used to allow for comparison with previously published studies (Grottoli et al. 2004, Rodrigues and Grottoli 2007, Levas et al. 2013). Soluble lipids (referred to hereafter simply as lipids) were extracted from a whole, ground coral sample (skeleton + animal tissue + algal endosymbiont) in a 2:1 chloroform:methanol solution for 1 hour (Grottoli et al. 2004, Rodrigues and Grottoli 2007), washed in 0.88% KCl followed by 100% chloroform and another wash with 0.88% KCl. The extract was dried to constant weight under a stream of pure nitrogen (UPH grade 5.0) and standardized to the ash-free dry weight.</p>
<p>Animal soluble protein and carbohydrate (referred to hereafter simply as protein and carbohydrate, respectively) were extracted from grinding a whole second branch tip of the same fragment (Rodrigues and Grottoli 2007). Briefly, Milli-Q water was added to the ground coral sample and the resulting slurry was sonicated (5 min) and then centrifuged twice (5000 rpm, 10 min) to separate the animal tissue from the skeleton and endosymbiotic algae. Protein and carbohydrate was extracted from the animal tissue only. One subsample of this animal tissue slurry was used for protein extraction using the bicinchoninic acid method (Smith et al. 1985) with bovine serum albumin as a standard (Pierce BCA Protein Assay Kit). A second subsample was used for carbohydrate quantification using the phenol-sulfuric acid method (Dubois et al. 1956) with glucose as a standard. Soluble animal protein and carbohydrate concentrations were standardized to the ash-free dry weight.</p>
<p>Tissue biomass was measured by drying a third branch tip of whole coral sample (skeleton + animal tissue + algal endosymbiont) to constant dry weight (24 hours, 60 degrees C) and burning it (6 hours, 450 degrees C). The difference between dry and burned weight was the ash free dry weight which was standardized to the surface area of this branch tip.</p>
<p><strong>Surface area. </strong>Surface area of plating <em>M. monasteriata</em> and <em>T. reniformis</em> fragments was determined using the aluminum foil technique (Marsh 1970), whereas surface area of branching <em>A. millepora</em> and <em>P. damicornis</em> fragments was determined using the single wax dipping technique (Stimson and Kinzie 1990, Veal et al. 2010) after the tissue had been removed. Natural wooden blocks of varying sizes and shapes were used as calibration standards (Veal et al. 2010). Wax dipping was conducted using household paraffin wax (Gulf Wax, Royal Oak Enterprises) heated to 65 degrees C. Dried coral skeletons and wooden calibration standards were maintained at room temperature prior to weighing.</p>
Specified by the Principal Investigator(s)
<p>Details of the statistical analysis methods are in Schoepf et al. 2013.</p>
<p>BCO-DMO Processing Notes:<br />
- Missing values indicated by 'nd' (no data)<br />
- Parameter names were modified to conform with BCO-DMO naming conventions<br />
- 31 July 2015: dataset re-served with values displaying 4 decimal places<br />
- 24 Sept 2015: new (corrected) version of dataset served; some protein and carbohydrate values were previously incorrect.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Nikon microphot-FXA epifluorescent microscope
Nikon microphot-FXA epifluorescent microscope
PI Supplied Instrument Name: Nikon microphot-FXA epifluorescent microscope PI Supplied Instrument Description:Endosymbiont quantification was calculated using 6 independent replicate counts on a hemocytometer, using a Nikon microphot-FXA epifluorescent microscope at 100X magnification. Instrument Name: Fluorescence Microscope Instrument Short Name: Instrument Description: Instruments that generate enlarged images of samples using the phenomena of fluorescence and phosphorescence instead of, or in addition to, reflection and absorption of visible light. Includes conventional and inverted instruments. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB06/
Hemocytometer
Hemocytometer
PI Supplied Instrument Name: Hemocytometer PI Supplied Instrument Description:Endosymbiont quantification was calculated using 6 independent replicate counts on a hemocytometer, using a Nikon microphot-FXA epifluorescent microscope at 100X magnification. Instrument Name: Hemocytometer Instrument Short Name:Hemocytometer Instrument Description: A hemocytometer is a small glass chamber, resembling a thick microscope slide, used for determining the number of cells per unit volume of a suspension. Originally used for performing blood cell counts, a hemocytometer can be used to count a variety of cell types in the laboratory. Also spelled as "haemocytometer". Description from:
http://hlsweb.dmu.ac.uk/ahs/elearning/RITA/Haem1/Haem1.html.
Deployment: Fiji_2011_Grottoli
Fiji_2011_Grottoli
Fiji
island
Fiji_2011_Grottoli
Andréa G. Grottoli
Ohio State University
Deployment: RSCF_Grottoli_2011
RSCF_Grottoli_2011
Reef Systems Coral Farm
laboratory
RSCF_Grottoli_2011
Andréa G. Grottoli
Ohio State University
Fiji
island